Rhodocytophaga rosea
Average proteome isoelectric point is 6.82
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 6909 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A6C0GHA4|A0A6C0GHA4_9BACT FtsX-like permease family protein OS=Rhodocytophaga rosea OX=2704465 GN=GXP67_12395 PE=4 SV=1
MM1 pKa = 6.15 QTFYY5 pKa = 11.4 LKK7 pKa = 10.75 NEE9 pKa = 4.22 YY10 pKa = 10.44 EE11 pKa = 4.52 DD12 pKa = 3.45 GTVFFKK18 pKa = 10.55 IEE20 pKa = 4.31 KK21 pKa = 8.81 IQNPEE26 pKa = 3.62 DD27 pKa = 3.67 EE28 pKa = 4.78 YY29 pKa = 10.72 IYY31 pKa = 10.88 DD32 pKa = 3.53 GTEE35 pKa = 3.4 IMIEE39 pKa = 3.96 EE40 pKa = 4.1 DD41 pKa = 3.72 TISKK45 pKa = 10.68 DD46 pKa = 3.31 EE47 pKa = 4.27 YY48 pKa = 10.91 EE49 pKa = 4.28 LTEE52 pKa = 4.19 EE53 pKa = 4.99 DD54 pKa = 4.02 LQEE57 pKa = 4.26 MYY59 pKa = 11.19 DD60 pKa = 3.8 DD61 pKa = 4.28 GFEE64 pKa = 4.19 VVPAAEE70 pKa = 4.14 YY71 pKa = 10.37 EE72 pKa = 4.28 EE73 pKa = 4.45 ADD75 pKa = 4.36 RR76 pKa = 11.84 RR77 pKa = 11.84 HH78 pKa = 5.69 QSLDD82 pKa = 3.27 LL83 pKa = 4.37
Molecular weight: 9.99 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.773
IPC2_protein 3.795
IPC_protein 3.732
Toseland 3.554
ProMoST 3.872
Dawson 3.694
Bjellqvist 3.859
Wikipedia 3.592
Rodwell 3.567
Grimsley 3.465
Solomon 3.681
Lehninger 3.63
Nozaki 3.821
DTASelect 3.948
Thurlkill 3.592
EMBOSS 3.605
Sillero 3.846
Patrickios 1.825
IPC_peptide 3.681
IPC2_peptide 3.821
IPC2.peptide.svr19 3.763
Protein with the highest isoelectric point:
>tr|A0A6C0GL21|A0A6C0GL21_9BACT Ribosomal RNA small subunit methyltransferase G OS=Rhodocytophaga rosea OX=2704465 GN=rsmG PE=3 SV=1
MM1 pKa = 7.45 KK2 pKa = 9.54 RR3 pKa = 11.84 TFQPSQRR10 pKa = 11.84 KK11 pKa = 8.72 RR12 pKa = 11.84 RR13 pKa = 11.84 NKK15 pKa = 9.3 HH16 pKa = 3.94 GFRR19 pKa = 11.84 EE20 pKa = 4.19 RR21 pKa = 11.84 MATANGRR28 pKa = 11.84 RR29 pKa = 11.84 VLASRR34 pKa = 11.84 RR35 pKa = 11.84 AKK37 pKa = 9.99 GRR39 pKa = 11.84 KK40 pKa = 7.37 NN41 pKa = 3.11
Molecular weight: 4.94 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.423
IPC2_protein 10.906
IPC_protein 12.501
Toseland 12.661
ProMoST 13.159
Dawson 12.661
Bjellqvist 12.661
Wikipedia 13.13
Rodwell 12.34
Grimsley 12.705
Solomon 13.159
Lehninger 13.056
Nozaki 12.661
DTASelect 12.661
Thurlkill 12.661
EMBOSS 13.159
Sillero 12.661
Patrickios 12.076
IPC_peptide 13.159
IPC2_peptide 12.149
IPC2.peptide.svr19 9.092
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
6909
0
6909
2421566
25
5275
350.5
39.38
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
7.29 ± 0.026
0.79 ± 0.01
4.865 ± 0.022
6.0 ± 0.032
4.822 ± 0.02
6.704 ± 0.031
1.986 ± 0.016
7.211 ± 0.029
6.593 ± 0.034
9.736 ± 0.037
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.219 ± 0.014
5.164 ± 0.03
3.993 ± 0.018
4.411 ± 0.022
4.077 ± 0.02
6.558 ± 0.03
5.987 ± 0.044
6.229 ± 0.025
1.264 ± 0.013
4.1 ± 0.023
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here