Paulinella micropora
Average proteome isoelectric point is 6.77
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 897 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A1L5YCZ3|A0A1L5YCZ3_9EUKA Glutaredoxin OS=Paulinella micropora OX=1928728 GN=MYN1_Chr_733 PE=3 SV=1
MM1 pKa = 7.31 LHH3 pKa = 7.07 LSNLLLAAVMWVQVPQWSADD23 pKa = 3.13 WSKK26 pKa = 11.17 CAVDD30 pKa = 5.23 LPDD33 pKa = 5.15 VNCHH37 pKa = 5.69 WYY39 pKa = 10.4 VVAPDD44 pKa = 3.48 NTFGEE49 pKa = 4.69 GFDD52 pKa = 3.64 WATSPWFDD60 pKa = 4.62 ANGLQDD66 pKa = 3.6 ISALHH71 pKa = 5.5 EE72 pKa = 4.34 TLTNLQNSS80 pKa = 3.94
Molecular weight: 9.0 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.797
IPC2_protein 3.999
IPC_protein 3.884
Toseland 3.681
ProMoST 4.101
Dawson 3.91
Bjellqvist 4.075
Wikipedia 3.91
Rodwell 3.732
Grimsley 3.605
Solomon 3.884
Lehninger 3.846
Nozaki 4.05
DTASelect 4.317
Thurlkill 3.783
EMBOSS 3.91
Sillero 4.024
Patrickios 0.693
IPC_peptide 3.884
IPC2_peptide 3.999
IPC2.peptide.svr19 3.894
Protein with the highest isoelectric point:
>tr|A0A1L5YCQ6|A0A1L5YCQ6_9EUKA 30S ribosomal protein S4 OS=Paulinella micropora OX=1928728 GN=rps4 PE=3 SV=1
MM1 pKa = 7.55 TKK3 pKa = 9.01 RR4 pKa = 11.84 TLGGTSRR11 pKa = 11.84 KK12 pKa = 9.07 RR13 pKa = 11.84 KK14 pKa = 8.17 RR15 pKa = 11.84 VSGFRR20 pKa = 11.84 VRR22 pKa = 11.84 MRR24 pKa = 11.84 SITGRR29 pKa = 11.84 RR30 pKa = 11.84 VIRR33 pKa = 11.84 TRR35 pKa = 11.84 RR36 pKa = 11.84 KK37 pKa = 8.74 RR38 pKa = 11.84 GRR40 pKa = 11.84 ARR42 pKa = 11.84 LSVV45 pKa = 3.2
Molecular weight: 5.34 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.523
IPC2_protein 11.389
IPC_protein 12.983
Toseland 13.144
ProMoST 13.642
Dawson 13.144
Bjellqvist 13.144
Wikipedia 13.627
Rodwell 12.749
Grimsley 13.188
Solomon 13.642
Lehninger 13.554
Nozaki 13.144
DTASelect 13.144
Thurlkill 13.144
EMBOSS 13.642
Sillero 13.144
Patrickios 12.486
IPC_peptide 13.656
IPC2_peptide 12.632
IPC2.peptide.svr19 9.284
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
897
0
897
272856
26
1535
304.2
33.88
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
7.371 ± 0.075
1.3 ± 0.026
4.719 ± 0.055
5.79 ± 0.071
3.967 ± 0.109
7.114 ± 0.07
2.086 ± 0.042
7.561 ± 0.068
4.556 ± 0.067
11.853 ± 0.1
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.06 ± 0.032
4.304 ± 0.047
4.461 ± 0.047
4.14 ± 0.048
5.885 ± 0.054
7.084 ± 0.065
5.315 ± 0.051
6.336 ± 0.061
1.418 ± 0.037
2.682 ± 0.04
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here