Alcube virus

Taxonomy: Viruses; Riboviria; Orthornavirae; Negarnaviricota; Polyploviricotina; Ellioviricetes; Bunyavirales; Phenuiviridae; Phlebovirus; Alcube phlebovirus

Average proteome isoelectric point is 6.49

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0N9MPA8|A0A0N9MPA8_9VIRU Nonstructural protein OS=Alcube virus OX=1725367 PE=4 SV=1
MM1 pKa = 7.39SLHH4 pKa = 5.84YY5 pKa = 10.46LYY7 pKa = 11.13DD8 pKa = 3.91RR9 pKa = 11.84VTPSYY14 pKa = 10.5DD15 pKa = 3.26YY16 pKa = 11.04PNIGEE21 pKa = 4.21VVANFVAVNSNVGLPVCVYY40 pKa = 11.23DD41 pKa = 5.02DD42 pKa = 5.83LEE44 pKa = 5.37FEE46 pKa = 4.6LAGHH50 pKa = 6.12RR51 pKa = 11.84QSLTYY56 pKa = 10.39RR57 pKa = 11.84EE58 pKa = 4.11TLFDD62 pKa = 5.75FIDD65 pKa = 3.93NDD67 pKa = 3.67EE68 pKa = 4.46MPWRR72 pKa = 11.84WGPRR76 pKa = 11.84LWGSRR81 pKa = 11.84VTLDD85 pKa = 3.96EE86 pKa = 4.55VPLLDD91 pKa = 4.45PLMKK95 pKa = 9.89TFQGLPVTDD104 pKa = 4.51LMDD107 pKa = 4.55PRR109 pKa = 11.84LHH111 pKa = 5.96FTRR114 pKa = 11.84NALFWPLDD122 pKa = 3.72HH123 pKa = 7.28VSLKK127 pKa = 10.62AFRR130 pKa = 11.84ILYY133 pKa = 7.18PCRR136 pKa = 11.84FVSFNSSRR144 pKa = 11.84RR145 pKa = 11.84EE146 pKa = 3.43LAEE149 pKa = 4.08YY150 pKa = 10.22IFEE153 pKa = 4.18ATRR156 pKa = 11.84APNLEE161 pKa = 4.17SGLVQLAKK169 pKa = 10.31KK170 pKa = 9.47VRR172 pKa = 11.84DD173 pKa = 3.55MALSLGLTEE182 pKa = 5.57RR183 pKa = 11.84LVPCKK188 pKa = 10.5DD189 pKa = 3.34IIKK192 pKa = 9.9SICILQFIRR201 pKa = 11.84MLKK204 pKa = 10.16GFQLDD209 pKa = 3.65RR210 pKa = 11.84EE211 pKa = 4.1RR212 pKa = 11.84SILQGGTLIPFMIGCTEE229 pKa = 3.71AVVRR233 pKa = 11.84DD234 pKa = 4.27FPGLISTWEE243 pKa = 3.75PRR245 pKa = 11.84LNVVDD250 pKa = 4.86FLHH253 pKa = 5.23VTPYY257 pKa = 11.13DD258 pKa = 3.67SGVSTDD264 pKa = 4.18SEE266 pKa = 4.22FDD268 pKa = 3.16SDD270 pKa = 4.69IEE272 pKa = 4.3VV273 pKa = 3.14

Molecular weight: 31.38 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0N9MPA8|A0A0N9MPA8_9VIRU Nonstructural protein OS=Alcube virus OX=1725367 PE=4 SV=1
MM1 pKa = 7.57SGQEE5 pKa = 4.1EE6 pKa = 4.18FAKK9 pKa = 10.44IAVEE13 pKa = 4.36FASEE17 pKa = 4.11AVDD20 pKa = 3.25TTLILEE26 pKa = 4.27IVKK29 pKa = 9.85EE30 pKa = 4.16FAYY33 pKa = 10.15QGYY36 pKa = 9.31DD37 pKa = 2.91AARR40 pKa = 11.84VIEE43 pKa = 3.95LVRR46 pKa = 11.84EE47 pKa = 4.2KK48 pKa = 11.12GGEE51 pKa = 3.74TWRR54 pKa = 11.84NDD56 pKa = 3.36VKK58 pKa = 10.65TMIIIALTRR67 pKa = 11.84GNKK70 pKa = 7.66PAKK73 pKa = 9.52ILEE76 pKa = 4.29KK77 pKa = 9.96MSVSGKK83 pKa = 7.83TKK85 pKa = 10.11FRR87 pKa = 11.84ALVLKK92 pKa = 10.89YY93 pKa = 10.15GLKK96 pKa = 10.29SGNPGRR102 pKa = 11.84DD103 pKa = 3.46DD104 pKa = 3.39LTLARR109 pKa = 11.84IASAFAAWTIQAIKK123 pKa = 10.41VVEE126 pKa = 4.66AYY128 pKa = 10.89LPVTGAAMDD137 pKa = 3.96EE138 pKa = 4.33LSPGFPRR145 pKa = 11.84PMMHH149 pKa = 6.95PCFGGLIDD157 pKa = 3.73NTLPEE162 pKa = 4.27EE163 pKa = 4.48TVASLVKK170 pKa = 10.49AHH172 pKa = 6.7SLFLDD177 pKa = 3.94AFSRR181 pKa = 11.84TINPSMRR188 pKa = 11.84TKK190 pKa = 10.44TKK192 pKa = 11.08AEE194 pKa = 3.9VAKK197 pKa = 10.96SFEE200 pKa = 4.22QPLNAAINSRR210 pKa = 11.84FLTSDD215 pKa = 3.59AKK217 pKa = 10.78RR218 pKa = 11.84KK219 pKa = 8.56ILKK222 pKa = 9.71SAGVIDD228 pKa = 4.63TNLKK232 pKa = 8.82PAPAVEE238 pKa = 4.09MAAKK242 pKa = 10.05KK243 pKa = 9.94FSEE246 pKa = 4.45MAA248 pKa = 3.73

Molecular weight: 27.06 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4

0

4

3948

248

2096

987.0

110.71

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.003 ± 1.234

2.66 ± 0.918

5.699 ± 0.394

6.307 ± 0.532

4.357 ± 0.448

5.927 ± 0.358

2.482 ± 0.375

6.104 ± 0.119

5.8 ± 0.529

9.271 ± 0.467

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

3.267 ± 0.527

3.647 ± 0.306

4.179 ± 0.391

2.913 ± 0.316

5.496 ± 0.59

9.093 ± 0.766

5.674 ± 0.247

7.042 ± 0.525

1.292 ± 0.129

2.786 ± 0.196
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski