Mumps orthorubulavirus
Average proteome isoelectric point is 7.16
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 8 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|Q9PX96|Q9PX96_9MONO Small hydrophobic protein OS=Mumps orthorubulavirus OX=2560602 GN=SH PE=2 SV=1
MM1 pKa = 7.49 SSVLKK6 pKa = 10.65 AFEE9 pKa = 4.31 RR10 pKa = 11.84 FTIEE14 pKa = 5.13 QEE16 pKa = 3.95 LQDD19 pKa = 3.9 RR20 pKa = 11.84 GEE22 pKa = 4.45 EE23 pKa = 4.26 GSIPPEE29 pKa = 4.03 TLKK32 pKa = 11.08 SAVKK36 pKa = 10.23 VFVINTPNPTTRR48 pKa = 11.84 YY49 pKa = 10.02 QMLNFCLRR57 pKa = 11.84 IICSQNARR65 pKa = 11.84 ASHH68 pKa = 6.07 RR69 pKa = 11.84 VGALITLFSLPSAGMQNHH87 pKa = 6.05 IRR89 pKa = 11.84 LADD92 pKa = 4.27 RR93 pKa = 11.84 SPEE96 pKa = 3.9 AQIEE100 pKa = 4.18 RR101 pKa = 11.84 CEE103 pKa = 4.02 IDD105 pKa = 3.34 GFEE108 pKa = 4.47 PGTYY112 pKa = 10.03 RR113 pKa = 11.84 LIPNARR119 pKa = 11.84 ANLTANEE126 pKa = 3.72 IAAYY130 pKa = 10.25 ALLADD135 pKa = 5.13 DD136 pKa = 6.14 LPPTINNGTPYY147 pKa = 10.34 VHH149 pKa = 7.43 ADD151 pKa = 3.72 VEE153 pKa = 4.47 GQPCDD158 pKa = 3.97 EE159 pKa = 4.8 IEE161 pKa = 4.09 QFLDD165 pKa = 2.71 RR166 pKa = 11.84 CYY168 pKa = 11.21 SVLIQAWVMVCKK180 pKa = 10.67 CMTAYY185 pKa = 9.79 DD186 pKa = 4.06 QPAGSADD193 pKa = 3.45 RR194 pKa = 11.84 RR195 pKa = 11.84 FAKK198 pKa = 10.22 YY199 pKa = 8.47 QQQGRR204 pKa = 11.84 LEE206 pKa = 4.21 ARR208 pKa = 11.84 YY209 pKa = 8.28 MLQPEE214 pKa = 4.46 AQRR217 pKa = 11.84 LIQTAIRR224 pKa = 11.84 KK225 pKa = 9.17 SLVVRR230 pKa = 11.84 QYY232 pKa = 10.98 LTFEE236 pKa = 4.12 LQLARR241 pKa = 11.84 RR242 pKa = 11.84 QGLLSNRR249 pKa = 11.84 YY250 pKa = 8.0 YY251 pKa = 11.82 AMVGDD256 pKa = 3.41 IGKK259 pKa = 10.26 YY260 pKa = 9.45 IEE262 pKa = 4.47 NSGLTAFFLTLKK274 pKa = 10.27 YY275 pKa = 10.86 ALGTKK280 pKa = 9.03 WSPLSLAAFTGEE292 pKa = 4.05 LTKK295 pKa = 10.79 LRR297 pKa = 11.84 SLMMLYY303 pKa = 10.24 RR304 pKa = 11.84 DD305 pKa = 3.69 LGEE308 pKa = 3.91 QARR311 pKa = 11.84 YY312 pKa = 8.75 LALLEE317 pKa = 4.89 APQIMDD323 pKa = 4.55 FAPGGYY329 pKa = 9.29 PLIFSYY335 pKa = 11.38 AMGVGTVLDD344 pKa = 3.74 VQMRR348 pKa = 11.84 NYY350 pKa = 9.87 TYY352 pKa = 11.25 ARR354 pKa = 11.84 PFLNGYY360 pKa = 7.57 YY361 pKa = 9.8 FQIGVEE367 pKa = 4.14 TARR370 pKa = 11.84 RR371 pKa = 11.84 QQGTVDD377 pKa = 3.5 NRR379 pKa = 11.84 VADD382 pKa = 4.32 DD383 pKa = 4.52 LGLTPEE389 pKa = 3.96 QRR391 pKa = 11.84 TEE393 pKa = 3.77 VTQLVDD399 pKa = 3.29 RR400 pKa = 11.84 LARR403 pKa = 11.84 GRR405 pKa = 11.84 GAGIPGGPVNPFVPPVQQQQPAAVYY430 pKa = 9.9 EE431 pKa = 4.72 DD432 pKa = 3.6 IPALEE437 pKa = 4.64 EE438 pKa = 4.19 SDD440 pKa = 5.43 DD441 pKa = 5.79 DD442 pKa = 4.71 GDD444 pKa = 3.87 EE445 pKa = 4.51 DD446 pKa = 5.2 GGAGFQNGAQAPAVRR461 pKa = 11.84 QGGQNDD467 pKa = 3.68 FRR469 pKa = 11.84 AQPLQDD475 pKa = 5.03 PIQAQLFMPLYY486 pKa = 8.72 PQVSNIPNHH495 pKa = 5.8 QNHH498 pKa = 6.27 QINRR502 pKa = 11.84 IGGMEE507 pKa = 4.03 HH508 pKa = 7.16 QDD510 pKa = 3.26 LLRR513 pKa = 11.84 YY514 pKa = 9.57 NEE516 pKa = 4.83 NGDD519 pKa = 3.68 SQQDD523 pKa = 3.16 ARR525 pKa = 11.84 GEE527 pKa = 4.14 HH528 pKa = 6.79 GNTFPNNPNQNAQSQVGDD546 pKa = 3.37 WDD548 pKa = 3.74 EE549 pKa = 3.83
Molecular weight: 61.37 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.974
IPC2_protein 5.067
IPC_protein 5.016
Toseland 4.927
ProMoST 5.092
Dawson 5.003
Bjellqvist 5.143
Wikipedia 4.863
Rodwell 4.902
Grimsley 4.851
Solomon 5.003
Lehninger 4.952
Nozaki 5.118
DTASelect 5.27
Thurlkill 4.927
EMBOSS 4.902
Sillero 5.181
Patrickios 3.821
IPC_peptide 5.003
IPC2_peptide 5.181
IPC2.peptide.svr19 5.111
Protein with the highest isoelectric point:
>tr|Q9DQA5|Q9DQA5_9MONO Nucleocapsid OS=Mumps orthorubulavirus OX=2560602 GN=NP PE=3 SV=1
MM1 pKa = 7.74 AGSQIKK7 pKa = 10.15 IPLPKK12 pKa = 10.27 PPDD15 pKa = 3.65 SDD17 pKa = 3.86 SQRR20 pKa = 11.84 LNAFPVIMAQEE31 pKa = 4.31 GKK33 pKa = 10.75 GRR35 pKa = 11.84 LLRR38 pKa = 11.84 QIRR41 pKa = 11.84 LRR43 pKa = 11.84 KK44 pKa = 9.07 ILSGDD49 pKa = 3.63 PSDD52 pKa = 3.94 QQITFVNTYY61 pKa = 9.45 GFIRR65 pKa = 11.84 ATPEE69 pKa = 3.38 TSEE72 pKa = 4.89 FISEE76 pKa = 4.1 SSQQKK81 pKa = 6.61 VTPVVTACMLSFGAGPVLEE100 pKa = 5.58 DD101 pKa = 3.75 PQHH104 pKa = 5.53 MLKK107 pKa = 10.72 ALDD110 pKa = 3.45 QTDD113 pKa = 2.67 IRR115 pKa = 11.84 VRR117 pKa = 11.84 KK118 pKa = 6.67 TASDD122 pKa = 3.51 KK123 pKa = 10.78 EE124 pKa = 4.24 QILFEE129 pKa = 4.59 INRR132 pKa = 11.84 IPNLFRR138 pKa = 11.84 HH139 pKa = 5.71 HH140 pKa = 6.5 QISADD145 pKa = 3.61 HH146 pKa = 7.21 LIQASSDD153 pKa = 3.64 KK154 pKa = 10.67 YY155 pKa = 10.17 VKK157 pKa = 10.71 SPAKK161 pKa = 10.41 LIAGVNYY168 pKa = 9.52 IYY170 pKa = 10.66 CVTFLSVTVCSASLKK185 pKa = 10.21 FRR187 pKa = 11.84 VARR190 pKa = 11.84 PLLAARR196 pKa = 11.84 SRR198 pKa = 11.84 LVRR201 pKa = 11.84 AVQMEE206 pKa = 3.98 VLLRR210 pKa = 11.84 VTCKK214 pKa = 10.17 KK215 pKa = 10.32 DD216 pKa = 3.28 SQMAKK221 pKa = 10.74 SMLNDD226 pKa = 4.02 PDD228 pKa = 4.43 GEE230 pKa = 4.48 GCIASVWFHH239 pKa = 6.66 LCNLCKK245 pKa = 10.4 GRR247 pKa = 11.84 NKK249 pKa = 10.08 LRR251 pKa = 11.84 SYY253 pKa = 10.72 DD254 pKa = 3.68 EE255 pKa = 4.94 NYY257 pKa = 10.07 FASKK261 pKa = 9.05 CRR263 pKa = 11.84 KK264 pKa = 8.57 MNLTVSIGDD273 pKa = 3.24 MWGPTILVHH282 pKa = 6.93 AGGHH286 pKa = 5.84 IPTTAKK292 pKa = 10.4 PFFNSRR298 pKa = 11.84 GWVCHH303 pKa = 6.67 PIHH306 pKa = 6.64 QSSPSLAKK314 pKa = 9.57 TLWSSGCEE322 pKa = 3.5 IKK324 pKa = 10.69 AASAILQGSDD334 pKa = 3.31 YY335 pKa = 11.56 ASLAKK340 pKa = 9.8 TDD342 pKa = 4.63 DD343 pKa = 3.84 IIYY346 pKa = 10.8 SKK348 pKa = 10.85 IKK350 pKa = 9.84 VDD352 pKa = 4.64 KK353 pKa = 10.99 DD354 pKa = 2.88 AANYY358 pKa = 10.05 KK359 pKa = 9.3 GVSWSPFRR367 pKa = 11.84 KK368 pKa = 9.21 SASMSNLL375 pKa = 3.11
Molecular weight: 41.58 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 8.96
IPC2_protein 8.799
IPC_protein 8.682
Toseland 9.531
ProMoST 9.253
Dawson 9.765
Bjellqvist 9.516
Wikipedia 9.897
Rodwell 10.16
Grimsley 9.809
Solomon 9.794
Lehninger 9.765
Nozaki 9.75
DTASelect 9.443
Thurlkill 9.648
EMBOSS 9.955
Sillero 9.75
Patrickios 5.69
IPC_peptide 9.794
IPC2_peptide 8.273
IPC2.peptide.svr19 7.941
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
7
1
8
4977
57
2261
622.1
69.39
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
6.631 ± 0.801
1.909 ± 0.313
4.521 ± 0.551
4.842 ± 0.551
3.456 ± 0.206
5.525 ± 0.876
1.828 ± 0.206
7.876 ± 0.776
4.28 ± 0.524
10.589 ± 1.188
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.311 ± 0.166
5.244 ± 0.515
5.385 ± 0.544
5.144 ± 0.782
5.023 ± 0.503
8.499 ± 0.761
6.57 ± 0.507
5.706 ± 0.297
1.125 ± 0.249
3.536 ± 0.359
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here