Torque teno midi virus 6
Average proteome isoelectric point is 7.07
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 4 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A7VLW0|A7VLW0_9VIRU Capsid protein OS=Torque teno midi virus 6 OX=2065047 PE=3 SV=1
MM1 pKa = 7.13 QNISAEE7 pKa = 4.14 DD8 pKa = 4.34 FYY10 pKa = 11.74 KK11 pKa = 9.89 EE12 pKa = 3.77 THH14 pKa = 5.91 FNQVTKK20 pKa = 10.09 HH21 pKa = 4.95 QLWMSIVTDD30 pKa = 3.48 AHH32 pKa = 7.37 DD33 pKa = 3.92 SVCNCWHH40 pKa = 7.21 PFAHH44 pKa = 6.13 MLANIFPPGHH54 pKa = 7.2 KK55 pKa = 10.11 DD56 pKa = 3.09 RR57 pKa = 11.84 NLTINQILEE66 pKa = 4.12 RR67 pKa = 11.84 DD68 pKa = 3.96 LKK70 pKa = 9.31 EE71 pKa = 3.99 TWPSGGDD78 pKa = 3.01 ADD80 pKa = 4.49 ASHH83 pKa = 6.68 GLVAGEE89 pKa = 4.25 DD90 pKa = 3.38 TGGEE94 pKa = 3.91 QIINPEE100 pKa = 4.16 EE101 pKa = 4.38 KK102 pKa = 9.96 EE103 pKa = 3.83 EE104 pKa = 4.08 EE105 pKa = 4.31 YY106 pKa = 10.57 IEE108 pKa = 5.11 DD109 pKa = 3.84 EE110 pKa = 5.11 EE111 pKa = 4.32 ITEE114 pKa = 4.26 LLKK117 pKa = 11.03 AADD120 pKa = 3.72 VAATRR125 pKa = 4.11
Molecular weight: 14.15 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.516
IPC2_protein 4.52
IPC_protein 4.444
Toseland 4.304
ProMoST 4.558
Dawson 4.393
Bjellqvist 4.546
Wikipedia 4.266
Rodwell 4.291
Grimsley 4.215
Solomon 4.393
Lehninger 4.342
Nozaki 4.507
DTASelect 4.647
Thurlkill 4.304
EMBOSS 4.279
Sillero 4.571
Patrickios 3.681
IPC_peptide 4.393
IPC2_peptide 4.558
IPC2.peptide.svr19 4.486
Protein with the highest isoelectric point:
>tr|A7VLW0|A7VLW0_9VIRU Capsid protein OS=Torque teno midi virus 6 OX=2065047 PE=3 SV=1
MM1 pKa = 7.28 AFWWRR6 pKa = 11.84 RR7 pKa = 11.84 RR8 pKa = 11.84 RR9 pKa = 11.84 KK10 pKa = 8.65 PWFGRR15 pKa = 11.84 WRR17 pKa = 11.84 RR18 pKa = 11.84 YY19 pKa = 8.61 RR20 pKa = 11.84 RR21 pKa = 11.84 RR22 pKa = 11.84 TNYY25 pKa = 9.37 KK26 pKa = 8.32 PRR28 pKa = 11.84 RR29 pKa = 11.84 KK30 pKa = 9.42 RR31 pKa = 11.84 RR32 pKa = 11.84 RR33 pKa = 11.84 IYY35 pKa = 10.01 RR36 pKa = 11.84 RR37 pKa = 11.84 RR38 pKa = 11.84 RR39 pKa = 11.84 NYY41 pKa = 8.44 RR42 pKa = 11.84 TTKK45 pKa = 8.14 GRR47 pKa = 11.84 RR48 pKa = 11.84 RR49 pKa = 11.84 RR50 pKa = 11.84 RR51 pKa = 11.84 YY52 pKa = 8.58 KK53 pKa = 10.44 VRR55 pKa = 11.84 RR56 pKa = 11.84 KK57 pKa = 6.7 QQKK60 pKa = 9.41 ILVKK64 pKa = 9.89 QWQPEE69 pKa = 4.43 CIRR72 pKa = 11.84 KK73 pKa = 9.54 CKK75 pKa = 10.19 IKK77 pKa = 10.83 GLGTIVLGAEE87 pKa = 4.3 GTQYY91 pKa = 10.98 RR92 pKa = 11.84 CYY94 pKa = 10.45 TDD96 pKa = 5.86 YY97 pKa = 11.43 LDD99 pKa = 3.85 EE100 pKa = 4.2 WTNPKK105 pKa = 10.05 VPGGGGFGCEE115 pKa = 4.06 LFTLRR120 pKa = 11.84 YY121 pKa = 8.47 LYY123 pKa = 11.14 KK124 pKa = 10.04 MYY126 pKa = 10.65 LARR129 pKa = 11.84 KK130 pKa = 8.94 NIWTFSNKK138 pKa = 9.81 YY139 pKa = 9.31 KK140 pKa = 10.9 DD141 pKa = 3.52 LVRR144 pKa = 11.84 YY145 pKa = 7.41 TGTRR149 pKa = 11.84 FTFFRR154 pKa = 11.84 HH155 pKa = 6.31 PEE157 pKa = 3.7 TDD159 pKa = 4.1 FILAYY164 pKa = 10.21 DD165 pKa = 4.06 IQPPFHH171 pKa = 5.84 ITKK174 pKa = 6.88 WTYY177 pKa = 10.62 MSIHH181 pKa = 6.44 PQMLLLRR188 pKa = 11.84 KK189 pKa = 9.18 HH190 pKa = 6.28 KK191 pKa = 10.52 KK192 pKa = 10.08 LLLSTATKK200 pKa = 10.5 PNGKK204 pKa = 8.62 LTTRR208 pKa = 11.84 FKK210 pKa = 10.59 IRR212 pKa = 11.84 PPKK215 pKa = 10.19 QLVTKK220 pKa = 9.8 WFFQKK225 pKa = 10.77 DD226 pKa = 3.34 FAQYY230 pKa = 11.02 GLVAIAAAACNLRR243 pKa = 11.84 YY244 pKa = 8.88 PWLACCNEE252 pKa = 3.76 NLIITLRR259 pKa = 11.84 YY260 pKa = 8.61 LQPQFYY266 pKa = 10.15 HH267 pKa = 6.29 NSAWAQTTKK276 pKa = 10.51 EE277 pKa = 4.02 YY278 pKa = 11.15 NPVYY282 pKa = 9.47 GTQPMKK288 pKa = 10.16 KK289 pKa = 7.61 TLTFNYY295 pKa = 9.85 IIGNQRR301 pKa = 11.84 GTYY304 pKa = 8.84 TMSNSDD310 pKa = 2.83 ISDD313 pKa = 3.55 YY314 pKa = 11.21 DD315 pKa = 3.84 SSVSISKK322 pKa = 9.91 GWFQPKK328 pKa = 9.4 ILNAYY333 pKa = 6.6 EE334 pKa = 4.4 VKK336 pKa = 10.85 DD337 pKa = 3.7 NTVTQALTPCGVLRR351 pKa = 11.84 YY352 pKa = 10.05 NPADD356 pKa = 5.09 DD357 pKa = 5.84 DD358 pKa = 4.4 GQGNKK363 pKa = 9.21 LWVVNTLTYY372 pKa = 9.76 NWKK375 pKa = 10.17 IPTDD379 pKa = 3.83 DD380 pKa = 4.34 DD381 pKa = 5.51 LILEE385 pKa = 5.53 GYY387 pKa = 8.49 PLWLMLYY394 pKa = 9.85 GYY396 pKa = 9.17 TSFLYY401 pKa = 9.84 QVKK404 pKa = 10.29 ADD406 pKa = 4.07 KK407 pKa = 11.4 NPFSSSLIVVKK418 pKa = 10.6 SSALQRR424 pKa = 11.84 VFGTDD429 pKa = 2.9 TTGWYY434 pKa = 9.53 PLIDD438 pKa = 3.66 KK439 pKa = 10.98 SFMDD443 pKa = 4.21 GKK445 pKa = 11.22 GPGNTDD451 pKa = 4.17 PILLHH456 pKa = 6.26 RR457 pKa = 11.84 KK458 pKa = 7.66 YY459 pKa = 9.57 WYY461 pKa = 9.27 PSLYY465 pKa = 9.75 GQRR468 pKa = 11.84 DD469 pKa = 4.12 SISQLVNCGPYY480 pKa = 8.62 VPKK483 pKa = 11.01 YY484 pKa = 10.85 NEE486 pKa = 4.45 TKK488 pKa = 10.85 NSTWQCNYY496 pKa = 10.43 KK497 pKa = 9.02 YY498 pKa = 9.33 TFYY501 pKa = 10.82 FKK503 pKa = 10.18 WGGHH507 pKa = 5.01 YY508 pKa = 10.29 PPEE511 pKa = 4.12 QEE513 pKa = 5.03 AEE515 pKa = 4.05 NPEE518 pKa = 4.15 KK519 pKa = 10.98 KK520 pKa = 10.27 NIYY523 pKa = 8.91 PVPDD527 pKa = 3.6 KK528 pKa = 10.81 QQEE531 pKa = 4.51 TISIADD537 pKa = 4.7 PISEE541 pKa = 4.5 KK542 pKa = 10.52 YY543 pKa = 9.42 DD544 pKa = 3.41 TVFRR548 pKa = 11.84 SWDD551 pKa = 3.37 YY552 pKa = 11.36 RR553 pKa = 11.84 RR554 pKa = 11.84 GSLTRR559 pKa = 11.84 TAIKK563 pKa = 10.34 RR564 pKa = 11.84 MQEE567 pKa = 3.77 NLEE570 pKa = 4.11 SDD572 pKa = 3.73 EE573 pKa = 4.51 SLSTDD578 pKa = 3.66 SAGCSSKK585 pKa = 10.67 KK586 pKa = 9.21 KK587 pKa = 10.34 KK588 pKa = 10.22 KK589 pKa = 10.2 YY590 pKa = 10.65 LPTLQDD596 pKa = 3.22 PKK598 pKa = 10.97 KK599 pKa = 9.97 EE600 pKa = 3.88 NKK602 pKa = 9.64 EE603 pKa = 3.77 IQKK606 pKa = 10.43 CLLSLCEE613 pKa = 3.99 EE614 pKa = 4.51 NTWQEE619 pKa = 4.02 PPQDD623 pKa = 4.09 SNILQLLQQQHH634 pKa = 5.14 QQQQSIKK641 pKa = 10.19 QNLLTLIADD650 pKa = 4.66 LKK652 pKa = 10.7 AKK654 pKa = 9.63 QRR656 pKa = 11.84 NLLHH660 pKa = 4.72 QTGYY664 pKa = 10.74 LAA666 pKa = 5.21
Molecular weight: 78.95 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 8.885
IPC2_protein 9.399
IPC_protein 9.355
Toseland 9.853
ProMoST 9.633
Dawson 10.116
Bjellqvist 9.838
Wikipedia 10.306
Rodwell 10.467
Grimsley 10.204
Solomon 10.131
Lehninger 10.087
Nozaki 9.897
DTASelect 9.809
Thurlkill 9.955
EMBOSS 10.277
Sillero 10.043
Patrickios 5.448
IPC_peptide 10.131
IPC2_peptide 8.58
IPC2.peptide.svr19 8.367
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
4
0
4
1322
125
666
330.5
38.4
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
5.976 ± 1.478
1.664 ± 0.212
4.841 ± 0.463
6.43 ± 1.872
3.555 ± 0.649
4.614 ± 0.585
3.026 ± 1.131
5.144 ± 0.389
8.396 ± 0.768
8.018 ± 0.77
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
1.74 ± 0.257
5.144 ± 0.098
5.976 ± 1.087
5.673 ± 0.774
6.278 ± 1.07
6.051 ± 1.657
7.564 ± 0.376
3.177 ± 0.208
2.345 ± 0.609
4.387 ± 1.687
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here