Aspergillus bombycis 
Average proteome isoelectric point is 6.32 
Get precalculated fractions of proteins 
 
  
    Acidic  
 
  
    pI < 6.8  
 
  
    6.8-7.4  
 
  
    pI > 7.4  
 
  
    Basic  
    
 
  
    All  
 
Note: above files contain also dissociation constants (pKa) 
Virtual 2D-PAGE plot for 12265 proteins (isoelectric point calculated using IPC2_protein) 
 
Get csv file with sequences according to given criteria: 
* You can choose from 21 different methods for calculating isoelectric point 
 Summary statistics related to proteome-wise predictions 
Protein with the lowest isoelectric point: 
>tr|A0A1F8AE64|A0A1F8AE64_9EURO Uncharacterized protein OS=Aspergillus bombycis OX=109264 GN=ABOM_001464 PE=4 SV=1MM1 pKa = 7.55  RR2 pKa = 11.84  LVYY5 pKa = 10.01  STVALTATALAVPVTVPSTYY25 pKa = 10.25  HH26 pKa = 5.5  RR27 pKa = 11.84  TSTVPGKK34 pKa = 10.4  VLEE37 pKa = 4.48  ASNPSDD43 pKa = 4.22  AGQLTARR50 pKa = 11.84  TFGPLINWLGQLKK63 pKa = 10.45  GGSSCEE69 pKa = 3.72  EE70 pKa = 4.3  CATGEE75 pKa = 4.22  RR76 pKa = 11.84  TGSDD80 pKa = 3.07  SMGVSIDD87 pKa = 4.94  SSAEE91 pKa = 3.8  DD92 pKa = 4.33  CNDD95 pKa = 3.26  EE96 pKa = 4.44  SSSNGNSNSNSNSNSNSNSNSNSNSNSNEE125 pKa = 3.47  NSNTNTSGGSTDD137 pKa = 3.71  SGSMPPGGMPPSGGTPPSGGTPPSGGMPPSGGTPPTGGTPPSGSTPPTSGTPPTGDD193 pKa = 3.22  VPTGSTPSGTPPTSGTEE210 pKa = 3.96  TGEE213 pKa = 4.11  EE214 pKa = 4.12  TGEE217 pKa = 4.18  EE218 pKa = 4.34  TGGSPPTSGTPPSGSTPPTSGTPPTGDD245 pKa = 3.17  VPTSGGTPPSSGTEE259 pKa = 3.8  TGITTGEE266 pKa = 4.01  EE267 pKa = 4.28  TGTSTGGSPPTGGKK281 pKa = 8.88  PPSGSTPPSSGTEE294 pKa = 3.58  NCEE297 pKa = 3.78  ACEE300 pKa = 4.06  KK301 pKa = 10.99  GNNSGNNSGSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSGGNHH354 pKa = 6.44  ASANASGPNDD364 pKa = 3.61  CAEE367 pKa = 4.64  CEE369 pKa = 4.36  VSVSVSPEE377 pKa = 3.64  DD378 pKa = 4.81  CEE380 pKa = 5.48  DD381 pKa = 3.82  FEE383 pKa = 4.94  
 36.37 kDa
Isoelectric point according different methods: 
IPC2.protein.svr19  3.75 
IPC2_protein 3.897 
IPC_protein 3.834 
Toseland    3.656 
ProMoST     3.935 
Dawson      3.783 
Bjellqvist  3.973 
Wikipedia   3.656 
Rodwell     3.668 
Grimsley    3.567 
Solomon     3.783 
Lehninger   3.732 
Nozaki      3.897 
DTASelect   4.024 
Thurlkill   3.681 
EMBOSS      3.681 
Sillero     3.948 
Patrickios  1.036 
IPC_peptide 3.783 
IPC2_peptide  3.935 
IPC2.peptide.svr19  3.856 
 Protein with the highest isoelectric point: 
>tr|A0A1F8A7Z5|A0A1F8A7Z5_9EURO ABM domain-containing protein OS=Aspergillus bombycis OX=109264 GN=ABOM_002712 PE=4 SV=1MM1 pKa = 7.61  PSALRR6 pKa = 11.84  TYY8 pKa = 10.02  SSPMSMSRR16 pKa = 11.84  YY17 pKa = 9.28  LSPKK21 pKa = 7.62  TTTTTPFSTLSSPLRR36 pKa = 11.84  PMTNFTTAIRR46 pKa = 11.84  PQLQTLSNTQLPSAATPSAQQSRR69 pKa = 11.84  SFSASASLAGKK80 pKa = 9.59  RR81 pKa = 11.84  ATYY84 pKa = 10.03  NPSRR88 pKa = 11.84  RR89 pKa = 11.84  VQKK92 pKa = 10.2  RR93 pKa = 11.84  RR94 pKa = 11.84  HH95 pKa = 5.18  GFLARR100 pKa = 11.84  VRR102 pKa = 11.84  SRR104 pKa = 11.84  GGRR107 pKa = 11.84  MIILRR112 pKa = 11.84  RR113 pKa = 11.84  RR114 pKa = 11.84  AKK116 pKa = 10.07  GRR118 pKa = 11.84  KK119 pKa = 8.12  SLSWW123 pKa = 3.4  
 13.7 kDa
Isoelectric point according different methods: 
IPC2.protein.svr19  9.379 
IPC2_protein 11.155 
IPC_protein 12.398 
Toseland    12.544 
ProMoST     13.042 
Dawson      12.544 
Bjellqvist  12.544 
Wikipedia   13.027 
Rodwell     12.179 
Grimsley    12.588 
Solomon     13.042 
Lehninger   12.954 
Nozaki      12.544 
DTASelect   12.544 
Thurlkill   12.544 
EMBOSS      13.042 
Sillero     12.544 
Patrickios  11.901 
IPC_peptide 13.056 
IPC2_peptide  12.032 
IPC2.peptide.svr19  9.064 
 Peptides (in silico  digests for buttom-up proteomics) 
Below you can find 
in silico  digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
 
  
    Try  
 
  
    ChTry  
 
  
    ArgC  
 
  
    LysN  
 
  
    TryLysC  
 
  
    Try  
 
  
    ChTry  
 
  
    ArgC  
 
  
    LysN  
 
  
    TryLysC  
 
  
    Try  
 
  
    ChTry  
 
  
    ArgC  
 
  
    LysN  
 
  
    TryLysC  
 
  
    Try  
 
  
    ChTry  
 
  
    ArgC  
 
  
    LysN  
 
  
    TryLysC  
 
  
    Try  
 
  
    ChTry  
 
  
    ArgC  
 
  
    LysN  
 
  
    TryLysC  
   
  
General Statistics 
    
      
        Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
 
     
    
      
        12265 
0
12265 
6082457
21
7757
495.9
54.97
          
     
  
 
    Amino acid frequency 
  
  
    
      
        Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
 
     
    
                 
        8.367 ± 0.019
1.314 ± 0.008
5.597 ± 0.013
6.043 ± 0.023
3.824 ± 0.013
6.828 ± 0.018
2.453 ± 0.008
5.117 ± 0.013
4.515 ± 0.018
9.252 ± 0.023
          
     
  
  
  
      
          
        Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
 
     
    
                 
        2.166 ± 0.006
3.673 ± 0.009
5.865 ± 0.023
4.043 ± 0.014
5.982 ± 0.019
8.229 ± 0.023
5.953 ± 0.014
6.321 ± 0.015
1.525 ± 0.009
2.933 ± 0.011
          
     
  
  
  
Note:  For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein levelMost of the basic statistics you can see at this page can be downloaded from this CSV file  
For dipeptide frequency statistics click here