Aspergillus bombycis
Average proteome isoelectric point is 6.32
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 12265 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A1F8AE64|A0A1F8AE64_9EURO Uncharacterized protein OS=Aspergillus bombycis OX=109264 GN=ABOM_001464 PE=4 SV=1
MM1 pKa = 7.55 RR2 pKa = 11.84 LVYY5 pKa = 10.01 STVALTATALAVPVTVPSTYY25 pKa = 10.25 HH26 pKa = 5.5 RR27 pKa = 11.84 TSTVPGKK34 pKa = 10.4 VLEE37 pKa = 4.48 ASNPSDD43 pKa = 4.22 AGQLTARR50 pKa = 11.84 TFGPLINWLGQLKK63 pKa = 10.45 GGSSCEE69 pKa = 3.72 EE70 pKa = 4.3 CATGEE75 pKa = 4.22 RR76 pKa = 11.84 TGSDD80 pKa = 3.07 SMGVSIDD87 pKa = 4.94 SSAEE91 pKa = 3.8 DD92 pKa = 4.33 CNDD95 pKa = 3.26 EE96 pKa = 4.44 SSSNGNSNSNSNSNSNSNSNSNSNSNSNEE125 pKa = 3.47 NSNTNTSGGSTDD137 pKa = 3.71 SGSMPPGGMPPSGGTPPSGGTPPSGGMPPSGGTPPTGGTPPSGSTPPTSGTPPTGDD193 pKa = 3.22 VPTGSTPSGTPPTSGTEE210 pKa = 3.96 TGEE213 pKa = 4.11 EE214 pKa = 4.12 TGEE217 pKa = 4.18 EE218 pKa = 4.34 TGGSPPTSGTPPSGSTPPTSGTPPTGDD245 pKa = 3.17 VPTSGGTPPSSGTEE259 pKa = 3.8 TGITTGEE266 pKa = 4.01 EE267 pKa = 4.28 TGTSTGGSPPTGGKK281 pKa = 8.88 PPSGSTPPSSGTEE294 pKa = 3.58 NCEE297 pKa = 3.78 ACEE300 pKa = 4.06 KK301 pKa = 10.99 GNNSGNNSGSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSGGNHH354 pKa = 6.44 ASANASGPNDD364 pKa = 3.61 CAEE367 pKa = 4.64 CEE369 pKa = 4.36 VSVSVSPEE377 pKa = 3.64 DD378 pKa = 4.81 CEE380 pKa = 5.48 DD381 pKa = 3.82 FEE383 pKa = 4.94
Molecular weight: 36.37 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.75
IPC2_protein 3.897
IPC_protein 3.834
Toseland 3.656
ProMoST 3.935
Dawson 3.783
Bjellqvist 3.973
Wikipedia 3.656
Rodwell 3.668
Grimsley 3.567
Solomon 3.783
Lehninger 3.732
Nozaki 3.897
DTASelect 4.024
Thurlkill 3.681
EMBOSS 3.681
Sillero 3.948
Patrickios 1.036
IPC_peptide 3.783
IPC2_peptide 3.935
IPC2.peptide.svr19 3.856
Protein with the highest isoelectric point:
>tr|A0A1F8A7Z5|A0A1F8A7Z5_9EURO ABM domain-containing protein OS=Aspergillus bombycis OX=109264 GN=ABOM_002712 PE=4 SV=1
MM1 pKa = 7.61 PSALRR6 pKa = 11.84 TYY8 pKa = 10.02 SSPMSMSRR16 pKa = 11.84 YY17 pKa = 9.28 LSPKK21 pKa = 7.62 TTTTTPFSTLSSPLRR36 pKa = 11.84 PMTNFTTAIRR46 pKa = 11.84 PQLQTLSNTQLPSAATPSAQQSRR69 pKa = 11.84 SFSASASLAGKK80 pKa = 9.59 RR81 pKa = 11.84 ATYY84 pKa = 10.03 NPSRR88 pKa = 11.84 RR89 pKa = 11.84 VQKK92 pKa = 10.2 RR93 pKa = 11.84 RR94 pKa = 11.84 HH95 pKa = 5.18 GFLARR100 pKa = 11.84 VRR102 pKa = 11.84 SRR104 pKa = 11.84 GGRR107 pKa = 11.84 MIILRR112 pKa = 11.84 RR113 pKa = 11.84 RR114 pKa = 11.84 AKK116 pKa = 10.07 GRR118 pKa = 11.84 KK119 pKa = 8.12 SLSWW123 pKa = 3.4
Molecular weight: 13.7 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.379
IPC2_protein 11.155
IPC_protein 12.398
Toseland 12.544
ProMoST 13.042
Dawson 12.544
Bjellqvist 12.544
Wikipedia 13.027
Rodwell 12.179
Grimsley 12.588
Solomon 13.042
Lehninger 12.954
Nozaki 12.544
DTASelect 12.544
Thurlkill 12.544
EMBOSS 13.042
Sillero 12.544
Patrickios 11.901
IPC_peptide 13.056
IPC2_peptide 12.032
IPC2.peptide.svr19 9.064
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
12265
0
12265
6082457
21
7757
495.9
54.97
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
8.367 ± 0.019
1.314 ± 0.008
5.597 ± 0.013
6.043 ± 0.023
3.824 ± 0.013
6.828 ± 0.018
2.453 ± 0.008
5.117 ± 0.013
4.515 ± 0.018
9.252 ± 0.023
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.166 ± 0.006
3.673 ± 0.009
5.865 ± 0.023
4.043 ± 0.014
5.982 ± 0.019
8.229 ± 0.023
5.953 ± 0.014
6.321 ± 0.015
1.525 ± 0.009
2.933 ± 0.011
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here