Lachnospiraceae bacterium

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Clostridia; Eubacteriales; Lachnospiraceae; unclassified Lachnospiraceae

Average proteome isoelectric point is 5.85

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3259 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1T5CBU8|A0A1T5CBU8_9FIRM Uncharacterized protein OS=Lachnospiraceae bacterium OX=1898203 GN=SAMN06296386_102177 PE=4 SV=1
MM1 pKa = 7.41KK2 pKa = 10.27KK3 pKa = 10.23KK4 pKa = 10.37LVSALLSATMVASLLAGCGNNASNSAPASEE34 pKa = 4.45AAPASEE40 pKa = 4.3AAPASEE46 pKa = 4.3AAPASEE52 pKa = 4.3AAPASEE58 pKa = 4.54AAPEE62 pKa = 3.95ANAAAGDD69 pKa = 3.96MTIGIAMPTKK79 pKa = 10.48SLEE82 pKa = 3.59RR83 pKa = 11.84WNRR86 pKa = 11.84DD87 pKa = 2.37GSYY90 pKa = 10.85LEE92 pKa = 4.28EE93 pKa = 4.03QFKK96 pKa = 11.1SRR98 pKa = 11.84GYY100 pKa = 10.1NVEE103 pKa = 3.94LTYY106 pKa = 11.03SDD108 pKa = 4.09NKK110 pKa = 10.15IDD112 pKa = 3.79QQVKK116 pKa = 10.46DD117 pKa = 3.95IEE119 pKa = 4.3GLIADD124 pKa = 4.36NVNLLVIAAIDD135 pKa = 4.09GEE137 pKa = 4.57SLSQVLAEE145 pKa = 4.61AKK147 pKa = 10.09EE148 pKa = 4.05SGIPVVSYY156 pKa = 11.22DD157 pKa = 3.78RR158 pKa = 11.84LIMNTDD164 pKa = 2.89AVSYY168 pKa = 9.96YY169 pKa = 11.15VSFDD173 pKa = 3.21NYY175 pKa = 10.76TVGTLQGQYY184 pKa = 10.93VIDD187 pKa = 5.21QLDD190 pKa = 3.91LDD192 pKa = 4.07NAGDD196 pKa = 3.37KK197 pKa = 10.17TYY199 pKa = 10.86NIEE202 pKa = 4.04FTAGDD207 pKa = 3.87PADD210 pKa = 3.81NNAGFFFNGAYY221 pKa = 7.94DD222 pKa = 3.69TLKK225 pKa = 10.53PYY227 pKa = 10.31IDD229 pKa = 4.67AGTLVIPSGQSTFDD243 pKa = 3.5QVATKK248 pKa = 10.21QWDD251 pKa = 3.47TATAMTRR258 pKa = 11.84MQNILASNYY267 pKa = 10.22SDD269 pKa = 3.39GTQLDD274 pKa = 3.79VALCSNDD281 pKa = 2.96STALGVTQAIEE292 pKa = 3.72SDD294 pKa = 3.9YY295 pKa = 11.6AGTNTVIITGQDD307 pKa = 2.89GDD309 pKa = 4.05EE310 pKa = 4.23ANLANVVDD318 pKa = 5.64GKK320 pKa = 11.17QSMTVYY326 pKa = 10.45KK327 pKa = 10.55AVANEE332 pKa = 3.94AVATLDD338 pKa = 4.26LADD341 pKa = 5.74AILKK345 pKa = 10.83GEE347 pKa = 4.24TPDD350 pKa = 4.49AGLIDD355 pKa = 3.75SAGWSFDD362 pKa = 3.29CAYY365 pKa = 9.91DD366 pKa = 3.7TEE368 pKa = 5.02SYY370 pKa = 11.68NNGTGIIPSYY380 pKa = 10.76LLVPTVVTTEE390 pKa = 3.87NLQKK394 pKa = 10.74EE395 pKa = 4.51LVDD398 pKa = 3.16TGYY401 pKa = 8.79YY402 pKa = 10.02TMGDD406 pKa = 3.5DD407 pKa = 5.33GYY409 pKa = 9.56PHH411 pKa = 7.46PVQQ414 pKa = 4.39

Molecular weight: 43.65 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1T5GLT3|A0A1T5GLT3_9FIRM Gamma-glutamyl phosphate reductase OS=Lachnospiraceae bacterium OX=1898203 GN=proA PE=3 SV=1
MM1 pKa = 7.72WMTFQPKK8 pKa = 9.16KK9 pKa = 8.53RR10 pKa = 11.84QRR12 pKa = 11.84SKK14 pKa = 9.17VHH16 pKa = 5.89GFRR19 pKa = 11.84ARR21 pKa = 11.84MSTPGGRR28 pKa = 11.84KK29 pKa = 8.8VLAARR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 10.26GRR39 pKa = 11.84AKK41 pKa = 10.1LTVV44 pKa = 3.04

Molecular weight: 5.08 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3259

0

3259

1122987

39

2305

344.6

38.58

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.539 ± 0.045

1.322 ± 0.016

6.456 ± 0.04

7.488 ± 0.042

4.3 ± 0.032

7.077 ± 0.04

1.748 ± 0.017

7.464 ± 0.037

6.779 ± 0.034

8.353 ± 0.044

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

3.053 ± 0.025

4.518 ± 0.033

3.258 ± 0.027

2.572 ± 0.02

4.472 ± 0.034

6.289 ± 0.038

5.345 ± 0.038

6.983 ± 0.033

0.892 ± 0.015

4.095 ± 0.033
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski