Lactococcus phage 53802

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; unclassified Siphoviridae

Average proteome isoelectric point is 6.34

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 58 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1P8BKZ0|A0A1P8BKZ0_9CAUD Intimate attachment protein OS=Lactococcus phage 53802 OX=1868850 GN=DS53802_41 PE=4 SV=1
MM1 pKa = 7.84SEE3 pKa = 4.32LEE5 pKa = 3.9KK6 pKa = 10.06TAPNEE11 pKa = 3.55IYY13 pKa = 10.69LIVGDD18 pKa = 4.38TDD20 pKa = 3.3TDD22 pKa = 4.33FNFNEE27 pKa = 4.29LEE29 pKa = 4.15EE30 pKa = 4.41VTWADD35 pKa = 3.23KK36 pKa = 10.64PIYY39 pKa = 10.03EE40 pKa = 4.23EE41 pKa = 3.92TAIKK45 pKa = 9.89YY46 pKa = 8.43VKK48 pKa = 10.19SSQLTIPKK56 pKa = 10.06SIADD60 pKa = 4.09EE61 pKa = 4.1LDD63 pKa = 2.84KK64 pKa = 11.27VTFHH68 pKa = 6.88NPIAQLSNKK77 pKa = 10.14DD78 pKa = 3.27CDD80 pKa = 3.71LTDD83 pKa = 4.24FNDD86 pKa = 3.72TVKK89 pKa = 10.67RR90 pKa = 11.84YY91 pKa = 9.07AWNNKK96 pKa = 7.41EE97 pKa = 3.71NGYY100 pKa = 9.75ICVAYY105 pKa = 9.01LTGKK109 pKa = 10.28ALGVDD114 pKa = 3.95LVKK117 pKa = 10.94VGEE120 pKa = 4.83GINDD124 pKa = 3.33NYY126 pKa = 11.47

Molecular weight: 14.26 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1P8BKV2|A0A1P8BKV2_9CAUD Replisome organizer protein OS=Lactococcus phage 53802 OX=1868850 GN=DS53802_14 PE=4 SV=1
MM1 pKa = 7.48LWSKK5 pKa = 10.76IKK7 pKa = 9.96TKK9 pKa = 10.61LVEE12 pKa = 4.3KK13 pKa = 11.04NMTEE17 pKa = 4.07YY18 pKa = 10.9EE19 pKa = 4.09LGKK22 pKa = 9.28VTGLGAQQIHH32 pKa = 5.27QFKK35 pKa = 10.17IGKK38 pKa = 6.82TAILSRR44 pKa = 11.84FYY46 pKa = 11.0FIFFLLLFIIVQHH59 pKa = 5.98RR60 pKa = 11.84QQNLSIWKK68 pKa = 9.76VLTT71 pKa = 3.73

Molecular weight: 8.43 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

58

0

58

11366

54

961

196.0

22.18

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.352 ± 0.387

0.449 ± 0.073

6.212 ± 0.213

7.478 ± 0.608

4.496 ± 0.21

6.44 ± 0.526

1.32 ± 0.15

6.995 ± 0.262

8.877 ± 0.463

7.848 ± 0.304

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.358 ± 0.144

6.124 ± 0.219

2.622 ± 0.184

4.461 ± 0.21

3.414 ± 0.203

6.678 ± 0.304

6.299 ± 0.262

6.247 ± 0.246

1.258 ± 0.116

4.074 ± 0.261
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski