Bacteriophage T5-like cott162

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Demerecviridae; Markadamsvirinae; Epseptimavirus; unclassified Epseptimavirus

Average proteome isoelectric point is 6.18

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 153 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A2K8HJ52|A0A2K8HJ52_9CAUD Uncharacterized protein OS=Bacteriophage T5-like cott162 OX=2024328 GN=P162_0055 PE=4 SV=1
MM1 pKa = 7.38KK2 pKa = 9.96TRR4 pKa = 11.84LDD6 pKa = 3.87CAVHH10 pKa = 6.55VLEE13 pKa = 5.31HH14 pKa = 6.83DD15 pKa = 4.02VVGTCMSYY23 pKa = 10.77HH24 pKa = 6.74EE25 pKa = 4.71AGKK28 pKa = 9.7MIVKK32 pKa = 10.11RR33 pKa = 11.84VADD36 pKa = 3.72VCSLKK41 pKa = 9.34ITEE44 pKa = 4.84DD45 pKa = 3.61EE46 pKa = 4.08ADD48 pKa = 3.79EE49 pKa = 4.66LLGQAIEE56 pKa = 4.01KK57 pKa = 9.95TEE59 pKa = 3.79IFLCDD64 pKa = 3.38SCSWWCEE71 pKa = 3.55AHH73 pKa = 5.81EE74 pKa = 4.85HH75 pKa = 6.14SFNDD79 pKa = 3.14YY80 pKa = 10.65DD81 pKa = 3.58VCRR84 pKa = 11.84DD85 pKa = 3.74CKK87 pKa = 10.89EE88 pKa = 4.18DD89 pKa = 5.51DD90 pKa = 3.56EE91 pKa = 6.95DD92 pKa = 3.85EE93 pKa = 4.25

Molecular weight: 10.69 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A2K8HQ00|A0A2K8HQ00_9CAUD Uncharacterized protein OS=Bacteriophage T5-like cott162 OX=2024328 GN=P162_0071 PE=4 SV=1
MM1 pKa = 8.0RR2 pKa = 11.84IYY4 pKa = 10.31EE5 pKa = 4.1NHH7 pKa = 6.0SFTRR11 pKa = 11.84IEE13 pKa = 4.0RR14 pKa = 11.84ASTLGKK20 pKa = 10.12LVSPLHH26 pKa = 5.95HH27 pKa = 6.68ARR29 pKa = 11.84MFDD32 pKa = 3.51KK33 pKa = 10.71EE34 pKa = 4.32SGVAIRR40 pKa = 11.84KK41 pKa = 7.39GAPTVAFTLL50 pKa = 4.01

Molecular weight: 5.64 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

153

0

153

29743

37

1235

194.4

21.86

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.968 ± 0.53

1.029 ± 0.105

6.291 ± 0.166

7.108 ± 0.246

4.256 ± 0.205

6.398 ± 0.213

1.812 ± 0.134

6.523 ± 0.209

7.266 ± 0.227

8.251 ± 0.188

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.76 ± 0.151

5.121 ± 0.171

3.376 ± 0.178

3.661 ± 0.191

4.378 ± 0.148

6.284 ± 0.206

5.931 ± 0.285

6.519 ± 0.176

1.241 ± 0.099

3.829 ± 0.161
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski