Sulfurovum sp. AR
Average proteome isoelectric point is 6.22
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 2114 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|I2K6X7|I2K6X7_9PROT Uncharacterized protein OS=Sulfurovum sp. AR OX=1165841 GN=SULAR_06408 PE=4 SV=1
MM1 pKa = 7.47 KK2 pKa = 10.29 LIKK5 pKa = 10.5 LIMILGALLLTNLSAAVISGYY26 pKa = 10.31 CFEE29 pKa = 5.39 DD30 pKa = 3.69 VNGDD34 pKa = 3.92 GIKK37 pKa = 10.82 DD38 pKa = 3.5 PGEE41 pKa = 4.02 ICAEE45 pKa = 3.88 EE46 pKa = 4.19 AVWAKK51 pKa = 10.09 IVNLSTGHH59 pKa = 6.18 VEE61 pKa = 4.01 VSKK64 pKa = 10.88 NLYY67 pKa = 10.62 SIDD70 pKa = 4.41 PEE72 pKa = 4.08 TSGYY76 pKa = 10.92 FEE78 pKa = 5.87 FNTHH82 pKa = 5.68 SKK84 pKa = 8.81 TGQFKK89 pKa = 11.05 VFLDD93 pKa = 3.73 NNADD97 pKa = 3.73 GKK99 pKa = 9.96 DD100 pKa = 3.66 TVATPPVNTHH110 pKa = 5.58 FTVDD114 pKa = 3.68 PLGDD118 pKa = 3.63 GTDD121 pKa = 3.56 TNRR124 pKa = 11.84 TNGEE128 pKa = 3.93 TYY130 pKa = 8.89 YY131 pKa = 10.69 TIDD134 pKa = 3.99 PGEE137 pKa = 4.25 EE138 pKa = 3.56 DD139 pKa = 3.96 TIYY142 pKa = 10.77 EE143 pKa = 4.06 NQDD146 pKa = 2.67 FGFQFGGDD154 pKa = 4.22 CVCEE158 pKa = 4.1 GADD161 pKa = 4.3 GIIIKK166 pKa = 8.78 KK167 pKa = 8.16 TININGDD174 pKa = 3.1 VSDD177 pKa = 4.25 WEE179 pKa = 4.6 SVHH182 pKa = 6.73 TDD184 pKa = 2.9 ADD186 pKa = 4.04 NNVCDD191 pKa = 5.06 HH192 pKa = 7.03 GDD194 pKa = 3.86 SEE196 pKa = 5.02 DD197 pKa = 3.61 KK198 pKa = 11.06 DD199 pKa = 5.41 AIVQSTGRR207 pKa = 11.84 NLVQFTWTGDD217 pKa = 2.91 NDD219 pKa = 3.32 FAYY222 pKa = 10.36 GFTRR226 pKa = 11.84 RR227 pKa = 11.84 VGSSTNTQTFIYY239 pKa = 10.12 YY240 pKa = 10.33 ADD242 pKa = 4.1 RR243 pKa = 11.84 DD244 pKa = 3.48 GDD246 pKa = 3.55 GRR248 pKa = 11.84 MEE250 pKa = 4.81 AEE252 pKa = 4.71 DD253 pKa = 3.82 FALVAGWQGNTGATSLYY270 pKa = 9.51 YY271 pKa = 10.45 YY272 pKa = 9.88 PYY274 pKa = 10.89 LPADD278 pKa = 4.25 LVNGDD283 pKa = 3.9 SMVWTQADD291 pKa = 3.06 IDD293 pKa = 4.09 AGKK296 pKa = 10.05 LPPGGQTPDD305 pKa = 3.43 TSWIGSGDD313 pKa = 3.92 GYY315 pKa = 9.73 TLAGNLDD322 pKa = 3.68 TGSGVDD328 pKa = 5.88 LIAAGNGLAQGVTEE342 pKa = 4.55 ASGGLDD348 pKa = 3.34 DD349 pKa = 6.31 AGVTMEE355 pKa = 4.37 WRR357 pKa = 11.84 VRR359 pKa = 11.84 WDD361 pKa = 4.02 AIGLTPFQGITYY373 pKa = 9.46 HH374 pKa = 6.5 ISTMNSSVNDD384 pKa = 3.79 NNPPGQVDD392 pKa = 4.23 DD393 pKa = 5.24 NMAGCYY399 pKa = 10.36 GEE401 pKa = 4.12 ATLRR405 pKa = 11.84 YY406 pKa = 9.38 CGVTLVPDD414 pKa = 3.99 RR415 pKa = 11.84 SIILPSGSVGPVYY428 pKa = 10.59 FEE430 pKa = 5.51 HH431 pKa = 7.24 NLTNTGNGDD440 pKa = 3.6 EE441 pKa = 4.66 NLTLSLTQTEE451 pKa = 4.31 SDD453 pKa = 3.66 FTVTYY458 pKa = 10.23 IEE460 pKa = 4.89 YY461 pKa = 11.08 YY462 pKa = 10.88 NDD464 pKa = 3.51 ANGNGVEE471 pKa = 5.02 DD472 pKa = 4.09 LGEE475 pKa = 4.26 EE476 pKa = 4.22 NLTGGPLALGAGEE489 pKa = 4.84 SINLLVKK496 pKa = 10.55 IGLPTGLDD504 pKa = 3.54 DD505 pKa = 6.02 NIATGEE511 pKa = 4.12 INATTTSCAVNKK523 pKa = 10.43 SAVVYY528 pKa = 9.82 DD529 pKa = 4.11 QIRR532 pKa = 11.84 VLPLNFDD539 pKa = 3.89 LDD541 pKa = 3.64 ITKK544 pKa = 10.3 EE545 pKa = 4.08 VSDD548 pKa = 5.72 DD549 pKa = 3.45 NASWVDD555 pKa = 3.83 TIEE558 pKa = 4.29 LSEE561 pKa = 4.51 GSPAYY566 pKa = 10.7 YY567 pKa = 10.44 RR568 pKa = 11.84 ITVTNNGPDD577 pKa = 3.32 GATDD581 pKa = 3.36 VTVNDD586 pKa = 3.7 VLPANVNYY594 pKa = 9.86 IGYY597 pKa = 9.28 YY598 pKa = 8.26 ATQGTYY604 pKa = 10.78 GAGQWMVGNLALDD617 pKa = 4.42 GSAEE621 pKa = 4.01 LFIDD625 pKa = 3.85 VNVSQTAGDD634 pKa = 3.58 LTVIQNIASTTDD646 pKa = 3.19 TNDD649 pKa = 3.05 TDD651 pKa = 5.16 TNDD654 pKa = 3.43 QDD656 pKa = 4.34 DD657 pKa = 4.36 ANITAIHH664 pKa = 6.26 IPVVNLQIVKK674 pKa = 8.8 TVSNPTPYY682 pKa = 10.31 EE683 pKa = 4.25 GEE685 pKa = 4.51 DD686 pKa = 3.05 INYY689 pKa = 7.68 TITVTKK695 pKa = 10.49 FGIDD699 pKa = 3.31 DD700 pKa = 4.01 AEE702 pKa = 3.99 NVVVNDD708 pKa = 4.34 ILPPWVSYY716 pKa = 11.18 VDD718 pKa = 3.59 STASVGSYY726 pKa = 11.14 DD727 pKa = 3.92 EE728 pKa = 4.44 TTGIWDD734 pKa = 3.77 IGTMSNSVEE743 pKa = 4.08 TLIIQATVDD752 pKa = 3.61 TNATLHH758 pKa = 5.79 NPIVNTASVSTDD770 pKa = 3.17 TTDD773 pKa = 3.16 TNPDD777 pKa = 3.59 DD778 pKa = 6.13 DD779 pKa = 4.07 IASATFNASNPADD792 pKa = 3.86 LGITKK797 pKa = 9.07 TVDD800 pKa = 3.12 NPNPQEE806 pKa = 4.08 GDD808 pKa = 3.67 TIVYY812 pKa = 7.58 TISVTNYY819 pKa = 9.91 GPLDD823 pKa = 3.44 ATNIYY828 pKa = 9.02 VQEE831 pKa = 4.16 EE832 pKa = 4.43 PFDD835 pKa = 4.44 GNLSITGTDD844 pKa = 3.22 TTLGTFNAPWWHH856 pKa = 5.91 IPEE859 pKa = 4.96 LNVSQTAVLTVTAMVDD875 pKa = 3.63 LNSTGDD881 pKa = 3.47 ILINNVIIDD890 pKa = 4.09 TEE892 pKa = 4.11 RR893 pKa = 11.84 LDD895 pKa = 4.22 QNDD898 pKa = 3.99 TNGEE902 pKa = 3.93 NDD904 pKa = 3.27 KK905 pKa = 10.18 ATAIVIVGCPCDD917 pKa = 4.58 NISSDD922 pKa = 4.11 SASAMNKK929 pKa = 8.49 TVGLLMICMTLLIGLIFVRR948 pKa = 11.84 RR949 pKa = 11.84 EE950 pKa = 3.34 EE951 pKa = 4.06 KK952 pKa = 10.45 YY953 pKa = 10.82 NRR955 pKa = 11.84 NEE957 pKa = 3.7 RR958 pKa = 3.68
Molecular weight: 102.59 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.706
IPC2_protein 3.681
IPC_protein 3.732
Toseland 3.503
ProMoST 3.897
Dawson 3.732
Bjellqvist 3.884
Wikipedia 3.668
Rodwell 3.554
Grimsley 3.401
Solomon 3.732
Lehninger 3.681
Nozaki 3.834
DTASelect 4.113
Thurlkill 3.554
EMBOSS 3.681
Sillero 3.859
Patrickios 0.782
IPC_peptide 3.719
IPC2_peptide 3.834
IPC2.peptide.svr19 3.749
Protein with the highest isoelectric point:
>tr|I2K7J7|I2K7J7_9PROT ATP synthase subunit a OS=Sulfurovum sp. AR OX=1165841 GN=atpB PE=3 SV=1
MM1 pKa = 7.28 KK2 pKa = 9.38 RR3 pKa = 11.84 TYY5 pKa = 9.96 QPHH8 pKa = 4.86 STPRR12 pKa = 11.84 KK13 pKa = 7.0 RR14 pKa = 11.84 THH16 pKa = 6.38 GFRR19 pKa = 11.84 TRR21 pKa = 11.84 MKK23 pKa = 8.87 TKK25 pKa = 10.11 NGRR28 pKa = 11.84 KK29 pKa = 9.33 VISARR34 pKa = 11.84 RR35 pKa = 11.84 AKK37 pKa = 9.87 GRR39 pKa = 11.84 KK40 pKa = 8.75 RR41 pKa = 11.84 LAVV44 pKa = 3.41
Molecular weight: 5.25 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.414
IPC2_protein 11.096
IPC_protein 12.457
Toseland 12.618
ProMoST 13.1
Dawson 12.618
Bjellqvist 12.603
Wikipedia 13.086
Rodwell 12.384
Grimsley 12.661
Solomon 13.1
Lehninger 13.013
Nozaki 12.618
DTASelect 12.603
Thurlkill 12.618
EMBOSS 13.115
Sillero 12.618
Patrickios 12.106
IPC_peptide 13.115
IPC2_peptide 12.091
IPC2.peptide.svr19 9.021
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
2114
0
2114
643284
29
2708
304.3
34.22
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
7.284 ± 0.065
0.872 ± 0.018
5.736 ± 0.048
7.278 ± 0.06
4.686 ± 0.038
6.454 ± 0.055
2.192 ± 0.027
7.859 ± 0.049
7.619 ± 0.064
9.77 ± 0.056
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.89 ± 0.027
4.356 ± 0.043
3.348 ± 0.031
3.093 ± 0.029
3.627 ± 0.036
6.102 ± 0.036
5.528 ± 0.045
6.503 ± 0.048
0.879 ± 0.02
3.925 ± 0.041
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here