Moraxella phage Mcat12
Average proteome isoelectric point is 6.77
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 37 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A0R6PHX8|A0A0R6PHX8_9CAUD CTP synthase (glutamine hydrolyzing) OS=Moraxella phage Mcat12 OX=1647527 PE=3 SV=1
MM1 pKa = 7.93 RR2 pKa = 11.84 YY3 pKa = 9.69 YY4 pKa = 11.02 YY5 pKa = 10.6 NEE7 pKa = 4.01 STDD10 pKa = 3.14 QYY12 pKa = 11.42 AKK14 pKa = 11.0 VLGEE18 pKa = 4.27 DD19 pKa = 3.07 AHH21 pKa = 7.38 SNLVTVIIDD30 pKa = 3.59 GKK32 pKa = 9.16 KK33 pKa = 9.8 QVMDD37 pKa = 3.16 WDD39 pKa = 4.05 EE40 pKa = 4.45 FVSQFNQLVDD50 pKa = 3.75 VYY52 pKa = 9.59 EE53 pKa = 4.72 DD54 pKa = 3.33 KK55 pKa = 11.12 SAEE58 pKa = 3.79 ISII61 pKa = 4.42
Molecular weight: 7.14 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.134
IPC2_protein 4.215
IPC_protein 4.101
Toseland 3.91
ProMoST 4.253
Dawson 4.075
Bjellqvist 4.24
Wikipedia 4.012
Rodwell 3.935
Grimsley 3.821
Solomon 4.062
Lehninger 4.024
Nozaki 4.202
DTASelect 4.406
Thurlkill 3.961
EMBOSS 4.024
Sillero 4.215
Patrickios 3.121
IPC_peptide 4.062
IPC2_peptide 4.202
IPC2.peptide.svr19 4.121
Protein with the highest isoelectric point:
>tr|A0A0R6PI17|A0A0R6PI17_9CAUD Uncharacterized protein OS=Moraxella phage Mcat12 OX=1647527 PE=4 SV=1
MM1 pKa = 7.25 QAGKK5 pKa = 10.59 LKK7 pKa = 10.86 DD8 pKa = 3.2 RR9 pKa = 11.84 VSFMQHH15 pKa = 4.97 LQSRR19 pKa = 11.84 SQTGATKK26 pKa = 9.55 RR27 pKa = 11.84 TWSVIDD33 pKa = 3.81 TVWGQFTPVSAKK45 pKa = 10.64 DD46 pKa = 3.65 VIAGKK51 pKa = 10.05 AAGVEE56 pKa = 3.65 ILARR60 pKa = 11.84 LKK62 pKa = 10.17 IRR64 pKa = 11.84 YY65 pKa = 9.21 RR66 pKa = 11.84 DD67 pKa = 3.92 DD68 pKa = 2.96 IKK70 pKa = 11.09 RR71 pKa = 11.84 NMRR74 pKa = 11.84 VVCRR78 pKa = 11.84 GKK80 pKa = 9.15 TYY82 pKa = 10.58 DD83 pKa = 4.32 IIGKK87 pKa = 8.93 PLADD91 pKa = 3.12 NKK93 pKa = 10.21 TGRR96 pKa = 11.84 EE97 pKa = 4.08 YY98 pKa = 10.47 LTLMLKK104 pKa = 10.19 EE105 pKa = 4.18 VLDD108 pKa = 4.28 DD109 pKa = 3.88 RR110 pKa = 11.84 QNN112 pKa = 3.0
Molecular weight: 12.77 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.17
IPC2_protein 9.414
IPC_protein 9.502
Toseland 10.394
ProMoST 9.926
Dawson 10.496
Bjellqvist 10.116
Wikipedia 10.628
Rodwell 11.008
Grimsley 10.54
Solomon 10.54
Lehninger 10.526
Nozaki 10.365
DTASelect 10.116
Thurlkill 10.394
EMBOSS 10.774
Sillero 10.409
Patrickios 10.745
IPC_peptide 10.555
IPC2_peptide 8.653
IPC2.peptide.svr19 8.532
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
37
0
37
8364
38
1174
226.1
25.17
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
9.254 ± 0.68
0.897 ± 0.199
5.99 ± 0.335
5.356 ± 0.359
3.922 ± 0.241
7.066 ± 0.39
2.571 ± 0.3
6.636 ± 0.281
6.982 ± 0.426
9.481 ± 0.377
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.774 ± 0.213
4.902 ± 0.28
3.109 ± 0.314
4.531 ± 0.297
4.376 ± 0.296
5.99 ± 0.409
5.524 ± 0.361
6.086 ± 0.364
1.196 ± 0.164
3.36 ± 0.28
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here