Vibrio phage K05K4_VK05K4_2

Taxonomy: Viruses; Monodnaviria; Loebvirae; Hofneiviricota; Faserviricetes; Tubulavirales; Inoviridae; unclassified Inoviridae

Average proteome isoelectric point is 6.49

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 20 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    

Protein with the lowest isoelectric point:
>tr|A0A1W6UG32|A0A1W6UG32_9VIRU Minor capsid protein OS=Vibrio phage K05K4_VK05K4_2 OX=1912327 GN=K05K4_52820 PE=4 SV=1
MM1 pKa = 7.29FLDD4 pKa = 3.39TGYY7 pKa = 11.16FDD9 pKa = 3.34TCTFEE14 pKa = 3.83KK15 pKa = 10.29TPYY18 pKa = 10.41SNARR22 pKa = 11.84YY23 pKa = 8.73PYY25 pKa = 9.25QTVCDD30 pKa = 3.76NGLGLGYY37 pKa = 10.63YY38 pKa = 7.75EE39 pKa = 4.43VRR41 pKa = 11.84CPEE44 pKa = 3.81NSEE47 pKa = 4.27FDD49 pKa = 3.99PSTLRR54 pKa = 11.84CKK56 pKa = 10.33SVCEE60 pKa = 4.04YY61 pKa = 11.13GKK63 pKa = 10.26NPDD66 pKa = 3.94GTCMDD71 pKa = 3.88ACQFKK76 pKa = 10.92KK77 pKa = 10.76SIDD80 pKa = 4.02EE81 pKa = 4.15IKK83 pKa = 10.48SLQWLAYY90 pKa = 9.88VYY92 pKa = 10.64GEE94 pKa = 4.11QVTGSCYY101 pKa = 10.68GDD103 pKa = 3.56YY104 pKa = 11.09GATRR108 pKa = 11.84CEE110 pKa = 4.25LEE112 pKa = 4.18RR113 pKa = 11.84TPSDD117 pKa = 3.21STLCTGVDD125 pKa = 3.31SGQWTQNTICHH136 pKa = 6.32GNFQFTGNQCEE147 pKa = 4.28GGTLFWGKK155 pKa = 10.11DD156 pKa = 3.62GPDD159 pKa = 3.31TPIIPDD165 pKa = 4.37DD166 pKa = 5.14PIHH169 pKa = 7.61DD170 pKa = 4.88PDD172 pKa = 5.88DD173 pKa = 3.89PTGDD177 pKa = 3.64IEE179 pKa = 5.54DD180 pKa = 4.5PSILPDD186 pKa = 3.61GSTNTVNPPDD196 pKa = 4.02TDD198 pKa = 3.65SEE200 pKa = 4.63PDD202 pKa = 3.48VEE204 pKa = 5.55EE205 pKa = 5.22PDD207 pKa = 3.63TDD209 pKa = 3.49EE210 pKa = 4.45STDD213 pKa = 3.64TAVLKK218 pKa = 10.91AITGMNKK225 pKa = 10.0DD226 pKa = 3.38VNKK229 pKa = 10.54ALNDD233 pKa = 3.47MNIDD237 pKa = 3.4INQANADD244 pKa = 3.75VQNQIIALNASMVTNTQAIQKK265 pKa = 7.84QQINDD270 pKa = 3.14NKK272 pKa = 10.37IYY274 pKa = 10.49EE275 pKa = 4.16NTKK278 pKa = 10.95ALIQQANADD287 pKa = 3.49ITTAMNKK294 pKa = 7.52NTNAVNGVGDD304 pKa = 4.45DD305 pKa = 3.72VEE307 pKa = 5.55KK308 pKa = 10.74IAGAMDD314 pKa = 5.45GIAEE318 pKa = 4.26DD319 pKa = 3.65VSGISDD325 pKa = 3.9TLDD328 pKa = 4.1GIANTDD334 pKa = 3.08TSGAGTGGTCIEE346 pKa = 4.43SQSCTGFYY354 pKa = 10.69EE355 pKa = 4.04SGYY358 pKa = 9.68PDD360 pKa = 3.62GLGGLVSGQLDD371 pKa = 3.73DD372 pKa = 5.58LKK374 pKa = 11.35HH375 pKa = 4.84NTIDD379 pKa = 3.42NFVNSFGDD387 pKa = 3.73LDD389 pKa = 4.02LSSAKK394 pKa = 10.1RR395 pKa = 11.84PSFVLPVPFFGDD407 pKa = 3.76FSFEE411 pKa = 4.02EE412 pKa = 4.39QISFDD417 pKa = 3.32WVFGFIRR424 pKa = 11.84AVLIMTSVFAARR436 pKa = 11.84RR437 pKa = 11.84IILEE441 pKa = 4.04VNMDD445 pKa = 3.55WLVDD449 pKa = 4.05LFNKK453 pKa = 10.38LLVFLYY459 pKa = 10.34QLLISLVNMLKK470 pKa = 10.66DD471 pKa = 3.49LFFGRR476 pKa = 11.84LSKK479 pKa = 10.26SWQWW483 pKa = 2.99

Molecular weight:
52.99 kDa
Isoelectric point according different methods:






Protein with the highest isoelectric point:
>tr|A0A1W6UH42|A0A1W6UH42_9VIRU Uncharacterized protein OS=Vibrio phage K05K4_VK05K4_2 OX=1912327 GN=K05K4_53010 PE=4 SV=1
MM1 pKa = 6.84VRR3 pKa = 11.84SVSRR7 pKa = 11.84SMPTPTKK14 pKa = 9.78WIRR17 pKa = 11.84RR18 pKa = 11.84YY19 pKa = 9.55LKK21 pKa = 9.52KK22 pKa = 10.75LGVRR26 pKa = 11.84RR27 pKa = 11.84VGKK30 pKa = 10.09SLSNGVLFTGDD41 pKa = 3.51AVTEE45 pKa = 4.07ILTCLQAKK53 pKa = 7.22QAKK56 pKa = 9.55NDD58 pKa = 4.19TIPFEE63 pKa = 4.71LNISALPILTWFRR76 pKa = 11.84SPLSLGTPTLEE87 pKa = 4.14AQYY90 pKa = 11.37SS91 pKa = 3.66

Molecular weight:
10.17 kDa
Isoelectric point according different methods:






Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

20

0

20

3264

61

483

163.2

18.32

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.893 ± 0.618

2.145 ± 0.392

6.434 ± 0.766

5.392 ± 0.314

4.32 ± 0.281

6.648 ± 0.448

1.961 ± 0.345

6.036 ± 0.38

6.005 ± 0.614

9.283 ± 0.735

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.911 ± 0.44

4.688 ± 0.604

4.044 ± 0.345

4.32 ± 0.561

4.381 ± 0.57

6.71 ± 0.464

6.311 ± 0.593

6.311 ± 0.384

1.409 ± 0.186

3.799 ± 0.412

Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski