Vibrio phage K05K4_VK05K4_2
Average proteome isoelectric point is 6.49
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 20 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A1W6UG32|A0A1W6UG32_9VIRU Minor capsid protein OS=Vibrio phage K05K4_VK05K4_2 OX=1912327 GN=K05K4_52820 PE=4 SV=1
MM1 pKa = 7.29 FLDD4 pKa = 3.39 TGYY7 pKa = 11.16 FDD9 pKa = 3.34 TCTFEE14 pKa = 3.83 KK15 pKa = 10.29 TPYY18 pKa = 10.41 SNARR22 pKa = 11.84 YY23 pKa = 8.73 PYY25 pKa = 9.25 QTVCDD30 pKa = 3.76 NGLGLGYY37 pKa = 10.63 YY38 pKa = 7.75 EE39 pKa = 4.43 VRR41 pKa = 11.84 CPEE44 pKa = 3.81 NSEE47 pKa = 4.27 FDD49 pKa = 3.99 PSTLRR54 pKa = 11.84 CKK56 pKa = 10.33 SVCEE60 pKa = 4.04 YY61 pKa = 11.13 GKK63 pKa = 10.26 NPDD66 pKa = 3.94 GTCMDD71 pKa = 3.88 ACQFKK76 pKa = 10.92 KK77 pKa = 10.76 SIDD80 pKa = 4.02 EE81 pKa = 4.15 IKK83 pKa = 10.48 SLQWLAYY90 pKa = 9.88 VYY92 pKa = 10.64 GEE94 pKa = 4.11 QVTGSCYY101 pKa = 10.68 GDD103 pKa = 3.56 YY104 pKa = 11.09 GATRR108 pKa = 11.84 CEE110 pKa = 4.25 LEE112 pKa = 4.18 RR113 pKa = 11.84 TPSDD117 pKa = 3.21 STLCTGVDD125 pKa = 3.31 SGQWTQNTICHH136 pKa = 6.32 GNFQFTGNQCEE147 pKa = 4.28 GGTLFWGKK155 pKa = 10.11 DD156 pKa = 3.62 GPDD159 pKa = 3.31 TPIIPDD165 pKa = 4.37 DD166 pKa = 5.14 PIHH169 pKa = 7.61 DD170 pKa = 4.88 PDD172 pKa = 5.88 DD173 pKa = 3.89 PTGDD177 pKa = 3.64 IEE179 pKa = 5.54 DD180 pKa = 4.5 PSILPDD186 pKa = 3.61 GSTNTVNPPDD196 pKa = 4.02 TDD198 pKa = 3.65 SEE200 pKa = 4.63 PDD202 pKa = 3.48 VEE204 pKa = 5.55 EE205 pKa = 5.22 PDD207 pKa = 3.63 TDD209 pKa = 3.49 EE210 pKa = 4.45 STDD213 pKa = 3.64 TAVLKK218 pKa = 10.91 AITGMNKK225 pKa = 10.0 DD226 pKa = 3.38 VNKK229 pKa = 10.54 ALNDD233 pKa = 3.47 MNIDD237 pKa = 3.4 INQANADD244 pKa = 3.75 VQNQIIALNASMVTNTQAIQKK265 pKa = 7.84 QQINDD270 pKa = 3.14 NKK272 pKa = 10.37 IYY274 pKa = 10.49 EE275 pKa = 4.16 NTKK278 pKa = 10.95 ALIQQANADD287 pKa = 3.49 ITTAMNKK294 pKa = 7.52 NTNAVNGVGDD304 pKa = 4.45 DD305 pKa = 3.72 VEE307 pKa = 5.55 KK308 pKa = 10.74 IAGAMDD314 pKa = 5.45 GIAEE318 pKa = 4.26 DD319 pKa = 3.65 VSGISDD325 pKa = 3.9 TLDD328 pKa = 4.1 GIANTDD334 pKa = 3.08 TSGAGTGGTCIEE346 pKa = 4.43 SQSCTGFYY354 pKa = 10.69 EE355 pKa = 4.04 SGYY358 pKa = 9.68 PDD360 pKa = 3.62 GLGGLVSGQLDD371 pKa = 3.73 DD372 pKa = 5.58 LKK374 pKa = 11.35 HH375 pKa = 4.84 NTIDD379 pKa = 3.42 NFVNSFGDD387 pKa = 3.73 LDD389 pKa = 4.02 LSSAKK394 pKa = 10.1 RR395 pKa = 11.84 PSFVLPVPFFGDD407 pKa = 3.76 FSFEE411 pKa = 4.02 EE412 pKa = 4.39 QISFDD417 pKa = 3.32 WVFGFIRR424 pKa = 11.84 AVLIMTSVFAARR436 pKa = 11.84 RR437 pKa = 11.84 IILEE441 pKa = 4.04 VNMDD445 pKa = 3.55 WLVDD449 pKa = 4.05 LFNKK453 pKa = 10.38 LLVFLYY459 pKa = 10.34 QLLISLVNMLKK470 pKa = 10.66 DD471 pKa = 3.49 LFFGRR476 pKa = 11.84 LSKK479 pKa = 10.26 SWQWW483 pKa = 2.99
Molecular weight: 52.99 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.889
IPC2_protein 3.91
IPC_protein 3.948
Toseland 3.719
ProMoST 4.101
Dawson 3.935
Bjellqvist 4.088
Wikipedia 3.872
Rodwell 3.77
Grimsley 3.617
Solomon 3.935
Lehninger 3.897
Nozaki 4.05
DTASelect 4.317
Thurlkill 3.77
EMBOSS 3.884
Sillero 4.062
Patrickios 2.664
IPC_peptide 3.935
IPC2_peptide 4.05
IPC2.peptide.svr19 3.976
Protein with the highest isoelectric point:
>tr|A0A1W6UH42|A0A1W6UH42_9VIRU Uncharacterized protein OS=Vibrio phage K05K4_VK05K4_2 OX=1912327 GN=K05K4_53010 PE=4 SV=1
MM1 pKa = 6.84 VRR3 pKa = 11.84 SVSRR7 pKa = 11.84 SMPTPTKK14 pKa = 9.78 WIRR17 pKa = 11.84 RR18 pKa = 11.84 YY19 pKa = 9.55 LKK21 pKa = 9.52 KK22 pKa = 10.75 LGVRR26 pKa = 11.84 RR27 pKa = 11.84 VGKK30 pKa = 10.09 SLSNGVLFTGDD41 pKa = 3.51 AVTEE45 pKa = 4.07 ILTCLQAKK53 pKa = 7.22 QAKK56 pKa = 9.55 NDD58 pKa = 4.19 TIPFEE63 pKa = 4.71 LNISALPILTWFRR76 pKa = 11.84 SPLSLGTPTLEE87 pKa = 4.14 AQYY90 pKa = 11.37 SS91 pKa = 3.66
Molecular weight: 10.17 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.302
IPC2_protein 9.692
IPC_protein 10.175
Toseland 10.701
ProMoST 10.277
Dawson 10.789
Bjellqvist 10.438
Wikipedia 10.95
Rodwell 11.169
Grimsley 10.818
Solomon 10.862
Lehninger 10.847
Nozaki 10.672
DTASelect 10.423
Thurlkill 10.687
EMBOSS 11.082
Sillero 10.701
Patrickios 10.95
IPC_peptide 10.877
IPC2_peptide 9.238
IPC2.peptide.svr19 8.655
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
20
0
20
3264
61
483
163.2
18.32
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
6.893 ± 0.618
2.145 ± 0.392
6.434 ± 0.766
5.392 ± 0.314
4.32 ± 0.281
6.648 ± 0.448
1.961 ± 0.345
6.036 ± 0.38
6.005 ± 0.614
9.283 ± 0.735
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.911 ± 0.44
4.688 ± 0.604
4.044 ± 0.345
4.32 ± 0.561
4.381 ± 0.57
6.71 ± 0.464
6.311 ± 0.593
6.311 ± 0.384
1.409 ± 0.186
3.799 ± 0.412
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here