Syncephalastrum racemosum (Filamentous fungus)

Taxonomy: cellular organisms; Eukaryota; Opisthokonta; Fungi; Fungi incertae sedis; Mucoromycota; Mucoromycotina; Mucoromycetes; Mucorales; Syncephalastraceae; Syncephalastrum

Average proteome isoelectric point is 6.69

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 11037 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1X2HQI1|A0A1X2HQI1_SYNRA Helicase C-terminal domain-containing protein (Fragment) OS=Syncephalastrum racemosum OX=13706 GN=BCR43DRAFT_542597 PE=4 SV=1
MM1 pKa = 7.33MPPLAKK7 pKa = 10.11TMVAAAAALLLGGNAVMSAPTNTAFPTGAPSAAASASGASAGASVAAKK55 pKa = 10.05ASKK58 pKa = 10.65GAGNSDD64 pKa = 3.67DD65 pKa = 5.34AFTNSTTPQVQITSDD80 pKa = 3.6TNFCLFMTPQPGLEE94 pKa = 3.97VATNEE99 pKa = 3.96EE100 pKa = 4.14NGIPFCTQANGVPNAKK116 pKa = 8.74TFPEE120 pKa = 4.48GFITTAHH127 pKa = 6.26YY128 pKa = 8.04EE129 pKa = 3.77QSTTYY134 pKa = 10.38VQVTGFFDD142 pKa = 3.34HH143 pKa = 6.78TKK145 pKa = 10.61YY146 pKa = 11.06GYY148 pKa = 11.13ADD150 pKa = 4.27DD151 pKa = 6.01DD152 pKa = 4.38GGGQYY157 pKa = 10.65DD158 pKa = 3.27SHH160 pKa = 8.04GNGKK164 pKa = 9.0PIAASCVGYY173 pKa = 9.33PYY175 pKa = 10.31FVSLLEE181 pKa = 3.89PSNNRR186 pKa = 11.84FCIRR190 pKa = 11.84CCQQEE195 pKa = 4.12DD196 pKa = 3.87DD197 pKa = 4.68CPTGRR202 pKa = 11.84SQYY205 pKa = 10.2GCLRR209 pKa = 11.84VIPGDD214 pKa = 3.68YY215 pKa = 9.51TQGPSSFDD223 pKa = 3.16NDD225 pKa = 3.18QAAGVLNKK233 pKa = 9.91VMEE236 pKa = 4.8DD237 pKa = 3.7FPTYY241 pKa = 10.1GQSIQQQGEE250 pKa = 4.11LDD252 pKa = 3.71EE253 pKa = 5.74LEE255 pKa = 4.19QAVAANTSAVQVQRR269 pKa = 11.84GFSNFNRR276 pKa = 11.84GLADD280 pKa = 3.48QNPNAQQDD288 pKa = 4.04LTQLTDD294 pKa = 3.51FTGNFSSVAQWTQFVQLLKK313 pKa = 10.33DD314 pKa = 3.83TIDD317 pKa = 3.96GGSANNDD324 pKa = 3.57QPSDD328 pKa = 3.69AAPSDD333 pKa = 3.88AAPSDD338 pKa = 3.88AAPSDD343 pKa = 3.88AAPSDD348 pKa = 3.88AAPSDD353 pKa = 3.75AAPSDD358 pKa = 3.97VAPSDD363 pKa = 3.8AAPSDD368 pKa = 3.88AAPSDD373 pKa = 3.89AAPSDD378 pKa = 3.96AQPSDD383 pKa = 3.25AQAPAGQPSDD393 pKa = 4.12AQPSAADD400 pKa = 3.98PSADD404 pKa = 3.61PAGGAPATSSPAAGAQEE421 pKa = 4.57PPAQQQQQQQQQPQQQQQQQPAAPAPAAAPPAAGAAAAPPQQ462 pKa = 3.55

Molecular weight: 46.92 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1X2HLV1|A0A1X2HLV1_SYNRA TNase-like domain-containing protein OS=Syncephalastrum racemosum OX=13706 GN=BCR43DRAFT_434868 PE=4 SV=1
MM1 pKa = 7.19VLRR4 pKa = 11.84RR5 pKa = 11.84SPRR8 pKa = 11.84RR9 pKa = 11.84LPLRR13 pKa = 11.84LPPRR17 pKa = 11.84RR18 pKa = 11.84LLRR21 pKa = 11.84HH22 pKa = 6.22RR23 pKa = 11.84LLLHH27 pKa = 6.29HH28 pKa = 7.08RR29 pKa = 11.84RR30 pKa = 11.84LLPQWLPRR38 pKa = 11.84QLLPPQLLLLLLLVHH53 pKa = 7.02RR54 pKa = 11.84LPLPRR59 pKa = 11.84LPVRR63 pKa = 11.84RR64 pKa = 11.84LLRR67 pKa = 11.84RR68 pKa = 11.84RR69 pKa = 11.84LPLRR73 pKa = 11.84PPSLNLPATSARR85 pKa = 11.84LRR87 pKa = 11.84LHH89 pKa = 6.85RR90 pKa = 11.84LLLLLHH96 pKa = 6.8RR97 pKa = 11.84LLLRR101 pKa = 11.84LRR103 pKa = 11.84PILSLPLRR111 pKa = 11.84RR112 pKa = 11.84PSTHH116 pKa = 5.9PRR118 pKa = 11.84HH119 pKa = 6.54RR120 pKa = 11.84ARR122 pKa = 11.84QLILRR127 pKa = 11.84NVAKK131 pKa = 10.14RR132 pKa = 11.84ASSSSSLRR140 pKa = 11.84CQRR143 pKa = 11.84MLWRR147 pKa = 11.84AA148 pKa = 3.14

Molecular weight: 17.87 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

11037

0

11037

4486539

49

6660

406.5

45.57

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.173 ± 0.025

1.436 ± 0.009

5.886 ± 0.016

6.118 ± 0.024

3.773 ± 0.016

5.542 ± 0.021

2.838 ± 0.012

4.926 ± 0.017

5.181 ± 0.023

9.142 ± 0.029

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.516 ± 0.01

3.787 ± 0.014

5.578 ± 0.027

4.863 ± 0.024

5.991 ± 0.018

7.823 ± 0.029

5.954 ± 0.016

6.115 ± 0.018

1.234 ± 0.008

3.126 ± 0.011
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski