Lynx canadensis faeces associated genomovirus CL1 48
Average proteome isoelectric point is 7.34
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 2 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A2Z5CJ90|A0A2Z5CJ90_9VIRU Replication-associated protein OS=Lynx canadensis faeces associated genomovirus CL1 48 OX=2219125 PE=3 SV=1
MM1 pKa = 7.88 PSFIANFRR9 pKa = 11.84 YY10 pKa = 10.32 ALVTYY15 pKa = 7.13 PQCGDD20 pKa = 3.69 LDD22 pKa = 3.61 PWSVMEE28 pKa = 4.79 HH29 pKa = 6.35 FSSLGAEE36 pKa = 4.26 CIIGRR41 pKa = 11.84 EE42 pKa = 4.04 HH43 pKa = 6.36 HH44 pKa = 6.38 QDD46 pKa = 3.4 GGSHH50 pKa = 5.84 LHH52 pKa = 5.75 VFVDD56 pKa = 4.53 FGRR59 pKa = 11.84 KK60 pKa = 7.86 FRR62 pKa = 11.84 SRR64 pKa = 11.84 ATDD67 pKa = 3.21 VFDD70 pKa = 4.92 VDD72 pKa = 3.6 GHH74 pKa = 6.46 HH75 pKa = 7.29 PNIEE79 pKa = 4.06 PSKK82 pKa = 8.78 GTPEE86 pKa = 4.01 KK87 pKa = 11.01 GFDD90 pKa = 3.46 YY91 pKa = 10.65 AIKK94 pKa = 10.71 DD95 pKa = 3.34 GDD97 pKa = 4.38 VICGGLGRR105 pKa = 11.84 PEE107 pKa = 4.21 PSRR110 pKa = 11.84 GSHH113 pKa = 6.06 SGMSAKK119 pKa = 9.3 WARR122 pKa = 11.84 ITGAADD128 pKa = 3.22 KK129 pKa = 11.36 QEE131 pKa = 4.22 FWDD134 pKa = 4.69 LVHH137 pKa = 6.98 EE138 pKa = 5.1 LDD140 pKa = 4.6 PKK142 pKa = 10.76 SAACSFTSLSKK153 pKa = 10.06 YY154 pKa = 10.56 ADD156 pKa = 2.96 WKK158 pKa = 10.74 FAVDD162 pKa = 3.64 PPGYY166 pKa = 9.12 EE167 pKa = 3.89 HH168 pKa = 7.63 PGGFNFSDD176 pKa = 3.99 GSFDD180 pKa = 4.13 GRR182 pKa = 11.84 SEE184 pKa = 4.2 CKK186 pKa = 10.23 SLVMYY191 pKa = 10.37 GPSRR195 pKa = 11.84 TGKK198 pKa = 8.21 TMWARR203 pKa = 11.84 SLGAHH208 pKa = 7.19 LYY210 pKa = 10.52 CVGLLSGDD218 pKa = 3.43 EE219 pKa = 4.17 CMKK222 pKa = 10.97 AEE224 pKa = 4.1 YY225 pKa = 10.57 ADD227 pKa = 3.67 YY228 pKa = 11.27 AVFDD232 pKa = 4.48 DD233 pKa = 4.63 LRR235 pKa = 11.84 GGFKK239 pKa = 10.44 FFPAFKK245 pKa = 9.22 EE246 pKa = 3.89 WLGAQQYY253 pKa = 7.12 VTVKK257 pKa = 10.2 RR258 pKa = 11.84 LYY260 pKa = 10.33 RR261 pKa = 11.84 EE262 pKa = 3.77 PRR264 pKa = 11.84 LVKK267 pKa = 9.28 WGKK270 pKa = 9.69 PSIFLTNSDD279 pKa = 4.04 PRR281 pKa = 11.84 DD282 pKa = 3.49 EE283 pKa = 4.4 ATVDD287 pKa = 3.4 DD288 pKa = 4.81 RR289 pKa = 11.84 DD290 pKa = 3.64 WLEE293 pKa = 4.06 RR294 pKa = 11.84 NCDD297 pKa = 4.42 FIYY300 pKa = 9.55 VQEE303 pKa = 5.02 PIFHH307 pKa = 7.21 ANTEE311 pKa = 4.14
Molecular weight: 35.03 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 5.425
IPC2_protein 5.385
IPC_protein 5.397
Toseland 5.715
ProMoST 5.601
Dawson 5.601
Bjellqvist 5.652
Wikipedia 5.575
Rodwell 5.563
Grimsley 5.83
Solomon 5.601
Lehninger 5.575
Nozaki 5.83
DTASelect 6.02
Thurlkill 5.957
EMBOSS 5.919
Sillero 5.919
Patrickios 3.923
IPC_peptide 5.614
IPC2_peptide 5.931
IPC2.peptide.svr19 5.896
Protein with the highest isoelectric point:
>tr|A0A2Z5CJ90|A0A2Z5CJ90_9VIRU Replication-associated protein OS=Lynx canadensis faeces associated genomovirus CL1 48 OX=2219125 PE=3 SV=1
MM1 pKa = 7.8 PARR4 pKa = 11.84 AYY6 pKa = 9.86 RR7 pKa = 11.84 RR8 pKa = 11.84 KK9 pKa = 9.34 SGSYY13 pKa = 9.45 RR14 pKa = 11.84 GRR16 pKa = 11.84 KK17 pKa = 9.06 RR18 pKa = 11.84 GGPTKK23 pKa = 10.37 RR24 pKa = 11.84 AWSNYY29 pKa = 8.11 KK30 pKa = 10.19 RR31 pKa = 11.84 STGRR35 pKa = 11.84 TRR37 pKa = 11.84 PYY39 pKa = 8.19 RR40 pKa = 11.84 TSRR43 pKa = 11.84 TRR45 pKa = 11.84 QKK47 pKa = 10.64 PMSRR51 pKa = 11.84 KK52 pKa = 9.74 KK53 pKa = 10.01 ILNVTSEE60 pKa = 4.03 KK61 pKa = 10.67 KK62 pKa = 9.49 RR63 pKa = 11.84 DD64 pKa = 4.56 KK65 pKa = 10.14 MLCWTNTIAGTPQGGTTYY83 pKa = 8.82 TQSPAILTGAGADD96 pKa = 3.47 PYY98 pKa = 11.28 LFAWCATARR107 pKa = 11.84 DD108 pKa = 4.19 NTTSTGGAAHH118 pKa = 7.57 AGTKK122 pKa = 9.38 FDD124 pKa = 3.59 KK125 pKa = 10.21 ATRR128 pKa = 11.84 TASEE132 pKa = 4.82 CYY134 pKa = 8.93 MVGLSEE140 pKa = 5.4 RR141 pKa = 11.84 IEE143 pKa = 4.04 IQCADD148 pKa = 3.69 GLPWQWRR155 pKa = 11.84 RR156 pKa = 11.84 ICFTMKK162 pKa = 10.51 GGVGLDD168 pKa = 3.5 GTLTTGSTFSSYY180 pKa = 11.46 SEE182 pKa = 4.15 TTSGYY187 pKa = 9.17 VRR189 pKa = 11.84 TVNSVLLTDD198 pKa = 3.86 RR199 pKa = 11.84 TSLYY203 pKa = 11.13 SLLFAGAQSSDD214 pKa = 2.6 WSDD217 pKa = 3.44 PMVAPLDD224 pKa = 3.53 RR225 pKa = 11.84 RR226 pKa = 11.84 RR227 pKa = 11.84 INVKK231 pKa = 9.65 YY232 pKa = 10.7 DD233 pKa = 2.94 KK234 pKa = 8.42 TTSISSGNEE243 pKa = 3.24 DD244 pKa = 3.33 GVIRR248 pKa = 11.84 KK249 pKa = 5.74 YY250 pKa = 9.15 TRR252 pKa = 11.84 WHH254 pKa = 6.58 PMRR257 pKa = 11.84 KK258 pKa = 7.21 TLVYY262 pKa = 10.83 DD263 pKa = 3.96 DD264 pKa = 5.44 DD265 pKa = 4.92 EE266 pKa = 4.56 NTGAEE271 pKa = 4.41 FPAVVSVDD279 pKa = 3.32 NKK281 pKa = 10.8 QGMGDD286 pKa = 3.48 YY287 pKa = 9.68 WVLDD291 pKa = 4.39 FIKK294 pKa = 10.35 PRR296 pKa = 11.84 MGSATSNQLSFRR308 pKa = 11.84 PEE310 pKa = 3.58 TTLYY314 pKa = 7.96 WHH316 pKa = 6.68 EE317 pKa = 4.11 RR318 pKa = 3.51
Molecular weight: 35.68 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.262
IPC2_protein 9.443
IPC_protein 9.648
Toseland 10.014
ProMoST 9.823
Dawson 10.248
Bjellqvist 9.955
Wikipedia 10.438
Rodwell 10.54
Grimsley 10.335
Solomon 10.277
Lehninger 10.233
Nozaki 10.043
DTASelect 9.94
Thurlkill 10.087
EMBOSS 10.423
Sillero 10.16
Patrickios 9.809
IPC_peptide 10.262
IPC2_peptide 8.814
IPC2.peptide.svr19 8.425
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
2
0
2
629
311
318
314.5
35.35
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
6.995 ± 0.051
2.067 ± 0.325
7.154 ± 0.982
4.452 ± 0.859
4.769 ± 1.481
9.221 ± 0.273
2.385 ± 0.947
3.18 ± 0.023
5.723 ± 0.165
5.882 ± 0.146
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.385 ± 0.293
2.544 ± 0.395
5.087 ± 0.45
2.226 ± 0.191
7.79 ± 1.287
8.426 ± 0.456
7.79 ± 2.733
4.928 ± 0.346
2.703 ± 0.084
4.293 ± 0.279
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here