Capybara microvirus Cap1_SP_160
Average proteome isoelectric point is 7.06
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 6 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A4P8W810|A0A4P8W810_9VIRU Nonstructural protein OS=Capybara microvirus Cap1_SP_160 OX=2585396 PE=4 SV=1
MM1 pKa = 7.71 RR2 pKa = 11.84 SVHH5 pKa = 5.51 TQPSLNSIEE14 pKa = 4.21 LALMTVARR22 pKa = 11.84 SALILASPCPSAFSLICEE40 pKa = 3.94 VSMQFRR46 pKa = 11.84 NSFTRR51 pKa = 11.84 VRR53 pKa = 11.84 VPAPACSDD61 pKa = 3.52 SLTVQSEE68 pKa = 4.03 ADD70 pKa = 3.41 SCEE73 pKa = 3.13 IGYY76 pKa = 9.59 ILNQHH81 pKa = 5.14 VRR83 pKa = 11.84 GLPVVGNALPPMWQDD98 pKa = 2.87 NTALPDD104 pKa = 3.5 YY105 pKa = 11.07 LEE107 pKa = 4.24 AQNRR111 pKa = 11.84 VVSARR116 pKa = 11.84 QSFEE120 pKa = 4.02 ALPSAIRR127 pKa = 11.84 ARR129 pKa = 11.84 FRR131 pKa = 11.84 NDD133 pKa = 3.3 PLEE136 pKa = 3.9 MLRR139 pKa = 11.84 FLSDD143 pKa = 3.09 AEE145 pKa = 4.15 NRR147 pKa = 11.84 SEE149 pKa = 4.04 AVRR152 pKa = 11.84 LGLVDD157 pKa = 5.06 DD158 pKa = 5.0 KK159 pKa = 10.46 PTAVTAEE166 pKa = 4.37 KK167 pKa = 10.26 PSASADD173 pKa = 3.4 AAQVPSTT180 pKa = 4.0
Molecular weight: 19.53 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 5.267
IPC2_protein 5.385
IPC_protein 5.258
Toseland 5.232
ProMoST 5.41
Dawson 5.283
Bjellqvist 5.385
Wikipedia 5.169
Rodwell 5.207
Grimsley 5.169
Solomon 5.27
Lehninger 5.232
Nozaki 5.41
DTASelect 5.563
Thurlkill 5.27
EMBOSS 5.232
Sillero 5.486
Patrickios 4.291
IPC_peptide 5.283
IPC2_peptide 5.486
IPC2.peptide.svr19 5.545
Protein with the highest isoelectric point:
>tr|A0A4P8W7Z6|A0A4P8W7Z6_9VIRU Minor capsid protein OS=Capybara microvirus Cap1_SP_160 OX=2585396 PE=4 SV=1
MM1 pKa = 7.64 GFGKK5 pKa = 10.09 SFKK8 pKa = 10.55 KK9 pKa = 9.78 IAKK12 pKa = 9.17 KK13 pKa = 9.28 VEE15 pKa = 3.48 RR16 pKa = 11.84 VYY18 pKa = 11.23 RR19 pKa = 11.84 NGRR22 pKa = 11.84 NAIPGYY28 pKa = 9.87 KK29 pKa = 9.69 QIFRR33 pKa = 11.84 SKK35 pKa = 9.92 PYY37 pKa = 10.35 KK38 pKa = 10.17 KK39 pKa = 10.05 LQHH42 pKa = 5.88 LVKK45 pKa = 10.71 KK46 pKa = 10.65 KK47 pKa = 10.96 GIDD50 pKa = 3.28 KK51 pKa = 10.72 AKK53 pKa = 10.6 KK54 pKa = 9.16 LGKK57 pKa = 10.24 ALFSPATTGGTAVIASYY74 pKa = 10.47 GANSAVAANRR84 pKa = 11.84 RR85 pKa = 11.84 TLSQMTGGNN94 pKa = 3.51
Molecular weight: 10.2 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.216
IPC2_protein 10.423
IPC_protein 11.023
Toseland 11.316
ProMoST 11.008
Dawson 11.359
Bjellqvist 11.052
Wikipedia 11.564
Rodwell 11.842
Grimsley 11.389
Solomon 11.52
Lehninger 11.477
Nozaki 11.272
DTASelect 11.052
Thurlkill 11.286
EMBOSS 11.711
Sillero 11.301
Patrickios 11.564
IPC_peptide 11.52
IPC2_peptide 9.794
IPC2.peptide.svr19 7.774
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
6
0
6
1458
83
548
243.0
27.12
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
8.436 ± 1.822
1.715 ± 0.569
5.898 ± 0.495
4.733 ± 0.703
5.281 ± 1.048
6.104 ± 0.726
1.852 ± 0.472
3.909 ± 0.5
5.624 ± 1.716
8.848 ± 0.406
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.058 ± 0.22
5.007 ± 0.684
5.007 ± 0.484
3.772 ± 0.581
6.584 ± 0.794
9.739 ± 1.081
3.772 ± 0.446
7.064 ± 0.98
1.029 ± 0.248
3.567 ± 0.798
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here