Thermothielavioides terrestris (strain ATCC 38088 / NRRL 8126) (Thielavia terrestris)
Average proteome isoelectric point is 6.73
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 9760 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|G2R4P1|G2R4P1_THETT Transcription initiation factor TFIID subunit 2 OS=Thermothielavioides terrestris (strain ATCC 38088 / NRRL 8126) OX=578455 GN=THITE_161557 PE=3 SV=1
MM1 pKa = 7.53 EE2 pKa = 5.18 PVRR5 pKa = 11.84 DD6 pKa = 4.03 NITAVDD12 pKa = 3.57 GDD14 pKa = 3.78 IAAVHH19 pKa = 6.33 ANSGDD24 pKa = 3.77 VPAVAAVPADD34 pKa = 3.48 VVVNGVAHH42 pKa = 6.31 NGSLGGRR49 pKa = 11.84 DD50 pKa = 3.42 TTQLSIRR57 pKa = 11.84 GVPLHH62 pKa = 6.83 RR63 pKa = 11.84 PLPHH67 pKa = 6.26 EE68 pKa = 4.69 HH69 pKa = 7.1 LSGLAPGTVLICGHH83 pKa = 5.93 MVCVPCWVAWGGDD96 pKa = 3.4 HH97 pKa = 7.48 VEE99 pKa = 4.82 FRR101 pKa = 11.84 AEE103 pKa = 3.95 VEE105 pKa = 4.2 NGSEE109 pKa = 4.06 YY110 pKa = 10.71 QEE112 pKa = 4.46 GDD114 pKa = 3.32 FDD116 pKa = 5.42 EE117 pKa = 6.42 GPDD120 pKa = 4.44 DD121 pKa = 5.76 NEE123 pKa = 3.98 QQLQEE128 pKa = 4.26 GEE130 pKa = 4.27 EE131 pKa = 4.05 EE132 pKa = 4.31 AEE134 pKa = 4.21 EE135 pKa = 5.83 GEE137 pKa = 5.57 DD138 pKa = 5.68 DD139 pKa = 5.11 DD140 pKa = 7.82 DD141 pKa = 6.87 DD142 pKa = 4.26 MLGQEE147 pKa = 4.9 HH148 pKa = 7.51 PDD150 pKa = 3.46 EE151 pKa = 5.75 NGDD154 pKa = 4.16 EE155 pKa = 5.31 DD156 pKa = 4.2 EE157 pKa = 4.35 QQQAGPDD164 pKa = 3.78 DD165 pKa = 3.96 QDD167 pKa = 4.36 DD168 pKa = 4.6 GEE170 pKa = 5.64 GEE172 pKa = 4.32 DD173 pKa = 3.69 EE174 pKa = 6.38 GEE176 pKa = 5.9 DD177 pKa = 3.64 EE178 pKa = 6.3 DD179 pKa = 4.73 EE180 pKa = 5.23 DD181 pKa = 3.61 EE182 pKa = 5.43 GEE184 pKa = 3.97 ISEE187 pKa = 4.18 VDD189 pKa = 3.12 EE190 pKa = 5.2 DD191 pKa = 4.38 QFPDD195 pKa = 4.42 PPPQPAYY202 pKa = 9.68 EE203 pKa = 3.99 WRR205 pKa = 11.84 PPMKK209 pKa = 10.49 CPVCQLVLEE218 pKa = 4.86 HH219 pKa = 7.11 PGCGCPILPEE229 pKa = 4.49 DD230 pKa = 4.32 MPPSLEE236 pKa = 3.97 EE237 pKa = 5.34 DD238 pKa = 3.19 EE239 pKa = 5.28 DD240 pKa = 4.19 AAGGRR245 pKa = 11.84 EE246 pKa = 4.04 YY247 pKa = 10.88 SYY249 pKa = 11.87 ADD251 pKa = 3.63 DD252 pKa = 3.21 QWARR256 pKa = 11.84 RR257 pKa = 11.84 YY258 pKa = 8.69 PQTLPEE264 pKa = 4.29 RR265 pKa = 11.84 ADD267 pKa = 3.59 GAVSPFCAPCAARR280 pKa = 11.84 IGGNN284 pKa = 3.18
Molecular weight: 30.66 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.705
IPC2_protein 3.694
IPC_protein 3.706
Toseland 3.503
ProMoST 3.859
Dawson 3.694
Bjellqvist 3.846
Wikipedia 3.592
Rodwell 3.541
Grimsley 3.414
Solomon 3.681
Lehninger 3.643
Nozaki 3.795
DTASelect 3.999
Thurlkill 3.541
EMBOSS 3.605
Sillero 3.834
Patrickios 1.303
IPC_peptide 3.681
IPC2_peptide 3.808
IPC2.peptide.svr19 3.736
Protein with the highest isoelectric point:
>tr|G2QUQ2|G2QUQ2_THETT Glutamate dehydrogenase OS=Thermothielavioides terrestris (strain ATCC 38088 / NRRL 8126) OX=578455 GN=THITE_2107687 PE=3 SV=1
MM1 pKa = 8.14 PIPIAIPVPIPVTTTTATANHH22 pKa = 6.72 KK23 pKa = 10.19 NLAIRR28 pKa = 11.84 TTPGPHH34 pKa = 6.18 QPPQHH39 pKa = 5.17 QTPLTNPNRR48 pKa = 11.84 NRR50 pKa = 11.84 PRR52 pKa = 11.84 RR53 pKa = 11.84 RR54 pKa = 11.84 RR55 pKa = 11.84 TTPSPTPTSTPTPTATATTTPTTPPAPAIQISGRR89 pKa = 11.84 RR90 pKa = 11.84 PPVRR94 pKa = 11.84 VGRR97 pKa = 4.03
Molecular weight: 10.36 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.522
IPC2_protein 11.228
IPC_protein 12.837
Toseland 12.998
ProMoST 13.51
Dawson 12.998
Bjellqvist 12.998
Wikipedia 13.481
Rodwell 12.544
Grimsley 13.042
Solomon 13.495
Lehninger 13.408
Nozaki 12.998
DTASelect 12.998
Thurlkill 12.998
EMBOSS 13.495
Sillero 12.998
Patrickios 12.281
IPC_peptide 13.51
IPC2_peptide 12.486
IPC2.peptide.svr19 9.213
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
9760
0
9760
4525313
49
8068
463.7
50.76
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
10.466 ± 0.025
1.188 ± 0.011
5.594 ± 0.018
6.062 ± 0.027
3.431 ± 0.014
7.522 ± 0.025
2.323 ± 0.011
4.016 ± 0.016
4.234 ± 0.022
8.92 ± 0.025
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
1.956 ± 0.009
3.08 ± 0.013
6.817 ± 0.032
3.905 ± 0.019
6.897 ± 0.023
7.704 ± 0.026
5.641 ± 0.02
6.291 ± 0.018
1.445 ± 0.009
2.511 ± 0.011
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here