Thermothielavioides terrestris (strain ATCC 38088 / NRRL 8126) (Thielavia terrestris)

Taxonomy: cellular organisms; Eukaryota; Opisthokonta; Fungi; Dikarya; Ascomycota; saccharomyceta; Pezizomycotina; leotiomyceta; sordariomyceta; Sordariomycetes; Sordariomycetidae; Sordariales; Chaetomiaceae; Thermothielavioides; Thermothielavioides terrestris

Average proteome isoelectric point is 6.73

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 9760 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    

Protein with the lowest isoelectric point:
>tr|G2R4P1|G2R4P1_THETT Transcription initiation factor TFIID subunit 2 OS=Thermothielavioides terrestris (strain ATCC 38088 / NRRL 8126) OX=578455 GN=THITE_161557 PE=3 SV=1
MM1 pKa = 7.53EE2 pKa = 5.18PVRR5 pKa = 11.84DD6 pKa = 4.03NITAVDD12 pKa = 3.57GDD14 pKa = 3.78IAAVHH19 pKa = 6.33ANSGDD24 pKa = 3.77VPAVAAVPADD34 pKa = 3.48VVVNGVAHH42 pKa = 6.31NGSLGGRR49 pKa = 11.84DD50 pKa = 3.42TTQLSIRR57 pKa = 11.84GVPLHH62 pKa = 6.83RR63 pKa = 11.84PLPHH67 pKa = 6.26EE68 pKa = 4.69HH69 pKa = 7.1LSGLAPGTVLICGHH83 pKa = 5.93MVCVPCWVAWGGDD96 pKa = 3.4HH97 pKa = 7.48VEE99 pKa = 4.82FRR101 pKa = 11.84AEE103 pKa = 3.95VEE105 pKa = 4.2NGSEE109 pKa = 4.06YY110 pKa = 10.71QEE112 pKa = 4.46GDD114 pKa = 3.32FDD116 pKa = 5.42EE117 pKa = 6.42GPDD120 pKa = 4.44DD121 pKa = 5.76NEE123 pKa = 3.98QQLQEE128 pKa = 4.26GEE130 pKa = 4.27EE131 pKa = 4.05EE132 pKa = 4.31AEE134 pKa = 4.21EE135 pKa = 5.83GEE137 pKa = 5.57DD138 pKa = 5.68DD139 pKa = 5.11DD140 pKa = 7.82DD141 pKa = 6.87DD142 pKa = 4.26MLGQEE147 pKa = 4.9HH148 pKa = 7.51PDD150 pKa = 3.46EE151 pKa = 5.75NGDD154 pKa = 4.16EE155 pKa = 5.31DD156 pKa = 4.2EE157 pKa = 4.35QQQAGPDD164 pKa = 3.78DD165 pKa = 3.96QDD167 pKa = 4.36DD168 pKa = 4.6GEE170 pKa = 5.64GEE172 pKa = 4.32DD173 pKa = 3.69EE174 pKa = 6.38GEE176 pKa = 5.9DD177 pKa = 3.64EE178 pKa = 6.3DD179 pKa = 4.73EE180 pKa = 5.23DD181 pKa = 3.61EE182 pKa = 5.43GEE184 pKa = 3.97ISEE187 pKa = 4.18VDD189 pKa = 3.12EE190 pKa = 5.2DD191 pKa = 4.38QFPDD195 pKa = 4.42PPPQPAYY202 pKa = 9.68EE203 pKa = 3.99WRR205 pKa = 11.84PPMKK209 pKa = 10.49CPVCQLVLEE218 pKa = 4.86HH219 pKa = 7.11PGCGCPILPEE229 pKa = 4.49DD230 pKa = 4.32MPPSLEE236 pKa = 3.97EE237 pKa = 5.34DD238 pKa = 3.19EE239 pKa = 5.28DD240 pKa = 4.19AAGGRR245 pKa = 11.84EE246 pKa = 4.04YY247 pKa = 10.88SYY249 pKa = 11.87ADD251 pKa = 3.63DD252 pKa = 3.21QWARR256 pKa = 11.84RR257 pKa = 11.84YY258 pKa = 8.69PQTLPEE264 pKa = 4.29RR265 pKa = 11.84ADD267 pKa = 3.59GAVSPFCAPCAARR280 pKa = 11.84IGGNN284 pKa = 3.18

Molecular weight:
30.66 kDa
Isoelectric point according different methods:






Protein with the highest isoelectric point:
>tr|G2QUQ2|G2QUQ2_THETT Glutamate dehydrogenase OS=Thermothielavioides terrestris (strain ATCC 38088 / NRRL 8126) OX=578455 GN=THITE_2107687 PE=3 SV=1
MM1 pKa = 8.14PIPIAIPVPIPVTTTTATANHH22 pKa = 6.72KK23 pKa = 10.19NLAIRR28 pKa = 11.84TTPGPHH34 pKa = 6.18QPPQHH39 pKa = 5.17QTPLTNPNRR48 pKa = 11.84NRR50 pKa = 11.84PRR52 pKa = 11.84RR53 pKa = 11.84RR54 pKa = 11.84RR55 pKa = 11.84TTPSPTPTSTPTPTATATTTPTTPPAPAIQISGRR89 pKa = 11.84RR90 pKa = 11.84PPVRR94 pKa = 11.84VGRR97 pKa = 4.03

Molecular weight:
10.36 kDa
Isoelectric point according different methods:






Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

9760

0

9760

4525313

49

8068

463.7

50.76

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

10.466 ± 0.025

1.188 ± 0.011

5.594 ± 0.018

6.062 ± 0.027

3.431 ± 0.014

7.522 ± 0.025

2.323 ± 0.011

4.016 ± 0.016

4.234 ± 0.022

8.92 ± 0.025

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.956 ± 0.009

3.08 ± 0.013

6.817 ± 0.032

3.905 ± 0.019

6.897 ± 0.023

7.704 ± 0.026

5.641 ± 0.02

6.291 ± 0.018

1.445 ± 0.009

2.511 ± 0.011

Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski