Nairobi sheep disease virus

Taxonomy: Viruses; Riboviria; Orthornavirae; Negarnaviricota; Polyploviricotina; Ellioviricetes; Bunyavirales; Nairoviridae; Orthonairovirus; Nairobi sheep disease orthonairovirus

Average proteome isoelectric point is 6.68

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    

Protein with the lowest isoelectric point:
>tr|Q8JYS4|Q8JYS4_9VIRU Nucleocapsid protein OS=Nairobi sheep disease virus OX=194540 PE=2 SV=1
MM1 pKa = 7.37ALVAKK6 pKa = 10.18GLILMLLLYY15 pKa = 8.73MFKK18 pKa = 10.63ASEE21 pKa = 4.36VNLEE25 pKa = 4.09LPSWNSTEE33 pKa = 3.91GTEE36 pKa = 4.67YY37 pKa = 11.08ADD39 pKa = 4.29EE40 pKa = 5.13LPTEE44 pKa = 4.55PPSEE48 pKa = 4.31EE49 pKa = 4.2EE50 pKa = 3.95PKK52 pKa = 10.44LAAVSTVTTPAPPKK66 pKa = 10.3EE67 pKa = 4.32STEE70 pKa = 4.07DD71 pKa = 3.64TIEE74 pKa = 4.43SPDD77 pKa = 3.56GGEE80 pKa = 4.11TMAPTTPAPVTDD92 pKa = 3.82SASKK96 pKa = 10.94LEE98 pKa = 4.35EE99 pKa = 4.28EE100 pKa = 5.31PGTTQQAQSTQMQNSQAPIGMPEE123 pKa = 3.93AWSTSPSRR131 pKa = 11.84VGRR134 pKa = 11.84KK135 pKa = 8.67PLSASPDD142 pKa = 3.35VSEE145 pKa = 4.9PDD147 pKa = 3.44QNTEE151 pKa = 4.01AQTHH155 pKa = 6.12PPVFLDD161 pKa = 3.4GRR163 pKa = 11.84PVEE166 pKa = 4.33EE167 pKa = 4.85EE168 pKa = 3.81EE169 pKa = 4.58HH170 pKa = 6.56SVGTRR175 pKa = 11.84DD176 pKa = 3.28VASDD180 pKa = 3.29LRR182 pKa = 11.84EE183 pKa = 3.95YY184 pKa = 11.38NQMKK188 pKa = 10.12QLLSQRR194 pKa = 11.84ILAKK198 pKa = 9.83MGMAVDD204 pKa = 4.54FTDD207 pKa = 5.72DD208 pKa = 5.75EE209 pKa = 5.71LDD211 pKa = 3.21TWCYY215 pKa = 10.24RR216 pKa = 11.84RR217 pKa = 11.84YY218 pKa = 10.69SNCSSNDD225 pKa = 2.86IEE227 pKa = 4.87SRR229 pKa = 11.84IRR231 pKa = 11.84DD232 pKa = 3.52FFLITDD238 pKa = 3.92RR239 pKa = 11.84SEE241 pKa = 4.8CFDD244 pKa = 3.48EE245 pKa = 4.85VLVKK249 pKa = 10.29RR250 pKa = 11.84LCEE253 pKa = 4.05TTSPIIDD260 pKa = 4.0RR261 pKa = 11.84AWKK264 pKa = 9.47IAGLKK269 pKa = 10.01EE270 pKa = 4.05EE271 pKa = 4.33VVLRR275 pKa = 11.84EE276 pKa = 3.47MGRR279 pKa = 11.84RR280 pKa = 11.84IFRR283 pKa = 11.84FFTPALKK290 pKa = 8.6VTCMSGSLNPSNQFVRR306 pKa = 11.84FYY308 pKa = 11.28NPTLEE313 pKa = 4.26RR314 pKa = 11.84TSGPTIQNYY323 pKa = 11.07DD324 pKa = 3.22GMHH327 pKa = 6.86CLNIEE332 pKa = 3.99NGLIKK337 pKa = 10.01PSKK340 pKa = 9.22VVVINVLMTTVDD352 pKa = 3.72IRR354 pKa = 11.84LEE356 pKa = 3.9SCRR359 pKa = 11.84AFINAQQCTYY369 pKa = 8.33TQHH372 pKa = 6.95ADD374 pKa = 3.05GLVRR378 pKa = 11.84VPTFVGPHH386 pKa = 4.93GEE388 pKa = 3.79KK389 pKa = 10.25RR390 pKa = 11.84IIGAYY395 pKa = 8.75TMSFNLTDD403 pKa = 4.34EE404 pKa = 4.42KK405 pKa = 11.55NKK407 pKa = 10.62ACTIKK412 pKa = 7.87TTCVVKK418 pKa = 10.83GKK420 pKa = 7.66EE421 pKa = 4.1VKK423 pKa = 10.22KK424 pKa = 10.64GQSQLRR430 pKa = 11.84GFPTTIRR437 pKa = 11.84LFKK440 pKa = 10.79SVTGKK445 pKa = 10.07RR446 pKa = 11.84RR447 pKa = 11.84LMSNEE452 pKa = 4.16EE453 pKa = 3.84EE454 pKa = 4.11WTEE457 pKa = 4.19CGSGTQLNMATAVSVHH473 pKa = 5.8NDD475 pKa = 2.92KK476 pKa = 11.39LGGPGKK482 pKa = 10.57KK483 pKa = 8.66LTICNGTTVSDD494 pKa = 3.36IALNEE499 pKa = 3.84GLGCYY504 pKa = 8.68TINKK508 pKa = 9.11IITGKK513 pKa = 9.43VCKK516 pKa = 9.65TGNTTGSCEE525 pKa = 4.07VQPEE529 pKa = 4.18LQKK532 pKa = 11.27CEE534 pKa = 3.89TGKK537 pKa = 11.05CILIKK542 pKa = 10.39QKK544 pKa = 11.11NKK546 pKa = 10.41GVVKK550 pKa = 10.35LKK552 pKa = 10.38RR553 pKa = 11.84GKK555 pKa = 8.14TVIITEE561 pKa = 4.35CQGSCLFAIPQDD573 pKa = 3.74TGDD576 pKa = 3.42ITIDD580 pKa = 3.46CSGGRR585 pKa = 11.84QHH587 pKa = 6.5YY588 pKa = 10.75LEE590 pKa = 4.61INIVDD595 pKa = 3.25IHH597 pKa = 7.08CPGKK601 pKa = 10.47DD602 pKa = 2.76KK603 pKa = 10.96WKK605 pKa = 11.01GFMLYY610 pKa = 9.37ICRR613 pKa = 11.84VSSRR617 pKa = 11.84PLVALTFGIWLTAGYY632 pKa = 10.14LITCLASFILYY643 pKa = 9.87NAILLISIAIKK654 pKa = 10.26KK655 pKa = 9.46LKK657 pKa = 9.65QKK659 pKa = 10.52RR660 pKa = 11.84EE661 pKa = 4.07KK662 pKa = 10.86KK663 pKa = 10.27GDD665 pKa = 3.58LCIKK669 pKa = 10.5CEE671 pKa = 3.93QHH673 pKa = 7.02CMNLYY678 pKa = 10.14DD679 pKa = 4.95QEE681 pKa = 4.32LHH683 pKa = 6.67EE684 pKa = 5.26LNCSFNLCPYY694 pKa = 9.27CANRR698 pKa = 11.84LSDD701 pKa = 4.41DD702 pKa = 3.57GLPRR706 pKa = 11.84HH707 pKa = 6.15VPRR710 pKa = 11.84CPKK713 pKa = 9.91RR714 pKa = 11.84GEE716 pKa = 3.84RR717 pKa = 11.84LEE719 pKa = 4.7EE720 pKa = 4.32IEE722 pKa = 5.83LYY724 pKa = 10.45INYY727 pKa = 8.36TRR729 pKa = 11.84VPCLLRR735 pKa = 11.84WALSTSVQVGTVIKK749 pKa = 9.65RR750 pKa = 11.84LSWFGVLLTLFFLTISPVQGFPLEE774 pKa = 4.18NPPIGTATDD783 pKa = 3.42SNIIYY788 pKa = 10.11GLAGFIFALVLMLNLKK804 pKa = 7.93KK805 pKa = 8.5THH807 pKa = 6.18YY808 pKa = 10.59GSVFHH813 pKa = 7.05VIDD816 pKa = 3.98GLGLCSLCDD825 pKa = 3.58TRR827 pKa = 11.84IDD829 pKa = 3.67SLMEE833 pKa = 3.76EE834 pKa = 4.47TAHH837 pKa = 6.1EE838 pKa = 4.36LCCAFDD844 pKa = 3.83CCPYY848 pKa = 9.62CHH850 pKa = 6.1QVSEE854 pKa = 5.11SNGSHH859 pKa = 6.55CFHH862 pKa = 6.65MRR864 pKa = 11.84ICVSKK869 pKa = 10.42EE870 pKa = 3.28ISKK873 pKa = 10.93KK874 pKa = 7.21MLKK877 pKa = 10.37AAGKK881 pKa = 10.23LFTNKK886 pKa = 9.62LVSRR890 pKa = 11.84EE891 pKa = 3.78GFFANKK897 pKa = 9.32IQLLYY902 pKa = 10.39QRR904 pKa = 11.84SKK906 pKa = 11.17VVSTLILTVLLLLIILKK923 pKa = 9.49PAAAFDD929 pKa = 4.37SGPLPDD935 pKa = 4.19GTWEE939 pKa = 4.11EE940 pKa = 4.38EE941 pKa = 3.73EE942 pKa = 4.32SLIRR946 pKa = 11.84SCDD949 pKa = 3.45QEE951 pKa = 4.63CVLEE955 pKa = 4.58QDD957 pKa = 3.6EE958 pKa = 4.71CLCPGEE964 pKa = 4.42HH965 pKa = 6.37RR966 pKa = 11.84AGRR969 pKa = 11.84RR970 pKa = 11.84LLFLSGLQDD979 pKa = 3.19AARR982 pKa = 11.84RR983 pKa = 11.84MTEE986 pKa = 3.44SHH988 pKa = 6.9RR989 pKa = 11.84LLTSVSIDD997 pKa = 3.8APWGAINIEE1006 pKa = 4.51STFKK1010 pKa = 10.81PLLAASNIDD1019 pKa = 3.82LSWNSAEE1026 pKa = 4.09EE1027 pKa = 3.99QGDD1030 pKa = 4.01KK1031 pKa = 10.61IVLSGRR1037 pKa = 11.84STGIIKK1043 pKa = 10.03LQEE1046 pKa = 3.89RR1047 pKa = 11.84TGLMWKK1053 pKa = 9.4MSSEE1057 pKa = 4.1KK1058 pKa = 10.66ASEE1061 pKa = 4.28SKK1063 pKa = 10.94NLLVSIMDD1071 pKa = 3.73FSQLYY1076 pKa = 10.39NSIFQYY1082 pKa = 9.28ITGDD1086 pKa = 3.21RR1087 pKa = 11.84SLSEE1091 pKa = 3.89WPKK1094 pKa = 10.34AVCTGEE1100 pKa = 4.17CPDD1103 pKa = 3.61RR1104 pKa = 11.84CGCQTSTCYY1113 pKa = 10.15HH1114 pKa = 6.6KK1115 pKa = 10.57EE1116 pKa = 3.85WPHH1119 pKa = 4.42TRR1121 pKa = 11.84NWRR1124 pKa = 11.84CNPTWCWGIGTGCTCCGMDD1143 pKa = 3.7VEE1145 pKa = 5.84RR1146 pKa = 11.84YY1147 pKa = 7.26FNKK1150 pKa = 10.26YY1151 pKa = 10.33FGVKK1155 pKa = 8.26WALEE1159 pKa = 4.03YY1160 pKa = 11.16VRR1162 pKa = 11.84TDD1164 pKa = 2.94VVVCVEE1170 pKa = 4.0LTNEE1174 pKa = 4.06EE1175 pKa = 4.28RR1176 pKa = 11.84HH1177 pKa = 6.17CDD1179 pKa = 3.38LVQAGSRR1186 pKa = 11.84FSIGPVSVTLSDD1198 pKa = 3.55PQNVVNRR1205 pKa = 11.84LSSSIMTIQEE1215 pKa = 3.56ISDD1218 pKa = 3.54NGILDD1223 pKa = 3.54VMHH1226 pKa = 6.31VSKK1229 pKa = 11.1VISAEE1234 pKa = 4.12NACKK1238 pKa = 10.25LQSCTHH1244 pKa = 6.8GSPGDD1249 pKa = 3.73LQILHH1254 pKa = 6.92TDD1256 pKa = 3.39NLIKK1260 pKa = 11.0GEE1262 pKa = 4.04MSSGINLAHH1271 pKa = 7.77LDD1273 pKa = 3.91PQVNTSWMSWEE1284 pKa = 4.43GCDD1287 pKa = 4.97LDD1289 pKa = 4.31YY1290 pKa = 11.25FCTVGDD1296 pKa = 4.36WPSCTYY1302 pKa = 10.48TGVNSINTDD1311 pKa = 2.77SFTNLINTEE1320 pKa = 3.55TDD1322 pKa = 3.52YY1323 pKa = 11.24TSKK1326 pKa = 10.53FHH1328 pKa = 6.01FHH1330 pKa = 6.78SKK1332 pKa = 10.14RR1333 pKa = 11.84ISARR1337 pKa = 11.84GDD1339 pKa = 3.44TLQMDD1344 pKa = 4.77LKK1346 pKa = 10.95ARR1348 pKa = 11.84PNVGGGEE1355 pKa = 3.99MTVLVEE1361 pKa = 4.19VNGLEE1366 pKa = 4.05LHH1368 pKa = 6.28SKK1370 pKa = 10.25KK1371 pKa = 10.32ISLKK1375 pKa = 10.22GLKK1378 pKa = 9.9LSNLKK1383 pKa = 10.75CSGCFACSPGLSCTVIARR1401 pKa = 11.84LEE1403 pKa = 4.31SPDD1406 pKa = 3.58EE1407 pKa = 4.02FTIHH1411 pKa = 6.32LRR1413 pKa = 11.84STSRR1417 pKa = 11.84DD1418 pKa = 3.38VVVAEE1423 pKa = 4.22TSVTARR1429 pKa = 11.84KK1430 pKa = 10.15AEE1432 pKa = 4.14TGAKK1436 pKa = 9.67SSFRR1440 pKa = 11.84AFAVKK1445 pKa = 9.85DD1446 pKa = 3.58VKK1448 pKa = 10.57EE1449 pKa = 3.86ICLEE1453 pKa = 4.12VVEE1456 pKa = 5.66KK1457 pKa = 9.81EE1458 pKa = 4.2YY1459 pKa = 11.35CPSCSIEE1466 pKa = 4.26DD1467 pKa = 3.65LKK1469 pKa = 11.19ICISVTLEE1477 pKa = 3.79PPKK1480 pKa = 10.7DD1481 pKa = 3.1ILIEE1485 pKa = 4.1HH1486 pKa = 7.2KK1487 pKa = 10.1GTIVQHH1493 pKa = 5.35YY1494 pKa = 9.29NKK1496 pKa = 10.52SCDD1499 pKa = 3.06EE1500 pKa = 5.3GYY1502 pKa = 10.54NCWIGSVSGFFVGVKK1517 pKa = 10.49DD1518 pKa = 4.16FFEE1521 pKa = 5.1KK1522 pKa = 10.97NFGSVIIGIVSTVLPLILTVLFFVYY1547 pKa = 10.17GRR1549 pKa = 11.84RR1550 pKa = 11.84LFCLCRR1556 pKa = 11.84LCHH1559 pKa = 6.38KK1560 pKa = 9.64KK1561 pKa = 10.3CCRR1564 pKa = 11.84GSSRR1568 pKa = 11.84GRR1570 pKa = 11.84DD1571 pKa = 3.51GYY1573 pKa = 11.57SRR1575 pKa = 11.84LSQEE1579 pKa = 4.18EE1580 pKa = 4.27EE1581 pKa = 3.44IKK1583 pKa = 10.82EE1584 pKa = 3.95IIKK1587 pKa = 10.41KK1588 pKa = 9.62FNRR1591 pKa = 11.84NGEE1594 pKa = 4.09LLGRR1598 pKa = 11.84GEE1600 pKa = 3.98NDD1602 pKa = 2.63KK1603 pKa = 10.37RR1604 pKa = 11.84TVARR1608 pKa = 11.84MFMDD1612 pKa = 3.42SQSARR1617 pKa = 11.84KK1618 pKa = 8.75AVKK1621 pKa = 9.73EE1622 pKa = 4.09VAA1624 pKa = 3.59

Molecular weight:
180.75 kDa
Isoelectric point according different methods:






Protein with the highest isoelectric point:
>tr|Q8JYS4|Q8JYS4_9VIRU Nucleocapsid protein OS=Nairobi sheep disease virus OX=194540 PE=2 SV=1
MM1 pKa = 7.23QNQIVADD8 pKa = 3.88NKK10 pKa = 10.55DD11 pKa = 4.68AILAWHH17 pKa = 6.0KK18 pKa = 9.71TYY20 pKa = 10.88SEE22 pKa = 3.87KK23 pKa = 10.88HH24 pKa = 5.56KK25 pKa = 10.79LKK27 pKa = 10.9SVLTNSASFCEE38 pKa = 4.54TIPDD42 pKa = 3.73LSGYY46 pKa = 7.55EE47 pKa = 3.67VSMRR51 pKa = 11.84LVSSEE56 pKa = 4.15SEE58 pKa = 3.91KK59 pKa = 11.23DD60 pKa = 3.42SVYY63 pKa = 11.32ASALVAATKK72 pKa = 10.1FCAPILEE79 pKa = 4.73CAWTSCTGMIQRR91 pKa = 11.84GLDD94 pKa = 3.0WFDD97 pKa = 4.29NNGEE101 pKa = 4.18MVKK104 pKa = 9.77IWDD107 pKa = 3.6ADD109 pKa = 3.7YY110 pKa = 11.22GKK112 pKa = 10.65LRR114 pKa = 11.84TEE116 pKa = 4.3VPSPEE121 pKa = 3.96QLLGYY126 pKa = 9.78QRR128 pKa = 11.84AALKK132 pKa = 8.96WRR134 pKa = 11.84KK135 pKa = 7.33DD136 pKa = 3.24TKK138 pKa = 11.14YY139 pKa = 10.94GINKK143 pKa = 7.32NTAALAAAIATEE155 pKa = 3.96YY156 pKa = 10.01RR157 pKa = 11.84VPGSIVVNVKK167 pKa = 10.83DD168 pKa = 4.23MLSDD172 pKa = 3.66MIRR175 pKa = 11.84RR176 pKa = 11.84RR177 pKa = 11.84NKK179 pKa = 9.35ILNRR183 pKa = 11.84DD184 pKa = 3.3GSEE187 pKa = 4.13DD188 pKa = 3.53VPKK191 pKa = 10.48RR192 pKa = 11.84GPVSKK197 pKa = 10.47EE198 pKa = 3.69HH199 pKa = 6.85IDD201 pKa = 3.36WARR204 pKa = 11.84DD205 pKa = 3.2LAQGKK210 pKa = 8.89FLVVFNPPWGDD221 pKa = 2.89INKK224 pKa = 9.86AGKK227 pKa = 10.2SGIALAATGMAKK239 pKa = 10.27LIEE242 pKa = 4.31LDD244 pKa = 3.77GPKK247 pKa = 10.03VAEE250 pKa = 4.5DD251 pKa = 3.56LKK253 pKa = 11.23EE254 pKa = 4.12SLKK257 pKa = 11.28SLVAWINAHH266 pKa = 6.49KK267 pKa = 10.99DD268 pKa = 3.15EE269 pKa = 4.42VEE271 pKa = 4.02NGKK274 pKa = 9.57EE275 pKa = 4.15VVDD278 pKa = 4.23GLTKK282 pKa = 10.39HH283 pKa = 5.57LQKK286 pKa = 10.99ALEE289 pKa = 4.06LAKK292 pKa = 10.53QSSAMRR298 pKa = 11.84AQGAQIDD305 pKa = 4.56TVFSSYY311 pKa = 10.14YY312 pKa = 8.24WLWKK316 pKa = 10.54AGVTAEE322 pKa = 4.09MFPTVSQFLFEE333 pKa = 4.73LGKK336 pKa = 10.56VPRR339 pKa = 11.84GNKK342 pKa = 9.16KK343 pKa = 8.62MKK345 pKa = 9.97KK346 pKa = 9.27ALSSMPLKK354 pKa = 9.92WGKK357 pKa = 10.36KK358 pKa = 9.14LLALFADD365 pKa = 4.76DD366 pKa = 5.22SFTANRR372 pKa = 11.84IYY374 pKa = 9.49MHH376 pKa = 7.05PGVLTAGRR384 pKa = 11.84MSEE387 pKa = 4.21LGVCFGAIPVANPDD401 pKa = 3.66DD402 pKa = 3.92AAEE405 pKa = 4.48GSGHH409 pKa = 6.62IKK411 pKa = 10.7NILNYY416 pKa = 9.06KK417 pKa = 9.42TDD419 pKa = 3.55TQAGNPCAQNIVALFNIQKK438 pKa = 10.36AGFDD442 pKa = 3.86IEE444 pKa = 5.06SMDD447 pKa = 3.3IVASEE452 pKa = 4.3HH453 pKa = 6.72LLHH456 pKa = 6.65QSLVGKK462 pKa = 10.49RR463 pKa = 11.84SPFQNAYY470 pKa = 10.19NIRR473 pKa = 11.84GNATSIQIII482 pKa = 3.82

Molecular weight:
53.1 kDa
Isoelectric point according different methods:






Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3

0

3

6097

482

3991

2032.3

228.91

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.084 ± 1.251

2.739 ± 0.88

5.33 ± 0.321

7.545 ± 0.634

3.904 ± 0.241

5.527 ± 0.764

1.771 ± 0.128

5.659 ± 0.346

7.446 ± 0.571

11.038 ± 0.964

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.296 ± 0.136

4.527 ± 0.208

3.313 ± 0.438

3.641 ± 0.24

5.15 ± 0.332

8.66 ± 0.386

6.347 ± 0.539

6.462 ± 0.029

1.165 ± 0.302

2.395 ± 0.08

Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski