Limihaloglobus sulfuriphilus

Taxonomy: cellular organisms; Bacteria; PVC group; Planctomycetes; Phycisphaerae; Sedimentisphaerales; Sedimentisphaeraceae; Limihaloglobus

Average proteome isoelectric point is 6.14

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2870 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1Q2ME07|A0A1Q2ME07_9BACT Endo-beta-mannanase OS=Limihaloglobus sulfuriphilus OX=1851148 GN=SMSP2_01289 PE=4 SV=1
MM1 pKa = 7.74KK2 pKa = 10.38LFQILTMFFFSLTLAVAVSADD23 pKa = 3.67LPAVNSGDD31 pKa = 3.21IFTHH35 pKa = 5.99VSADD39 pKa = 3.22SGVYY43 pKa = 8.79IQQGGSQASDD53 pKa = 3.41GDD55 pKa = 4.33LVGYY59 pKa = 8.62WQDD62 pKa = 3.46QSGNGNNANNFDD74 pKa = 4.02STGEE78 pKa = 4.14SKK80 pKa = 9.12PTFLDD85 pKa = 3.25NAVNGKK91 pKa = 8.84PVLRR95 pKa = 11.84FFGANYY101 pKa = 10.54LDD103 pKa = 4.59FGFNQYY109 pKa = 7.88LTQPNTVFLVAYY121 pKa = 9.97NLGEE125 pKa = 3.81NGYY128 pKa = 9.57YY129 pKa = 9.42IDD131 pKa = 6.52GITSDD136 pKa = 3.99DD137 pKa = 3.43RR138 pKa = 11.84QAFMVADD145 pKa = 4.58GLNVMWSGSYY155 pKa = 9.7FWQDD159 pKa = 2.64HH160 pKa = 5.62SKK162 pKa = 9.55PRR164 pKa = 11.84TWINHH169 pKa = 4.38TLVYY173 pKa = 10.73NDD175 pKa = 4.36TSSQYY180 pKa = 8.71YY181 pKa = 8.82TDD183 pKa = 4.32GVLQAAGSAGASNFGWGLRR202 pKa = 11.84LGSRR206 pKa = 11.84YY207 pKa = 10.15SGSLYY212 pKa = 9.98GWFYY216 pKa = 8.26VAEE219 pKa = 4.25MIIYY223 pKa = 8.98EE224 pKa = 4.3GSLTEE229 pKa = 4.75SDD231 pKa = 2.92ITLVNDD237 pKa = 3.77YY238 pKa = 10.92LSQKK242 pKa = 10.52YY243 pKa = 10.4NITGEE248 pKa = 3.89EE249 pKa = 4.15SQPVNTGAGTLVAQFDD265 pKa = 4.35GTDD268 pKa = 3.07ITADD272 pKa = 3.54GTNVLVWNDD281 pKa = 3.26QVDD284 pKa = 4.02DD285 pKa = 3.56VDD287 pKa = 4.29YY288 pKa = 10.65PLDD291 pKa = 3.77LAAVEE296 pKa = 4.38VDD298 pKa = 3.27MGDD301 pKa = 3.06ASGYY305 pKa = 8.56PQLEE309 pKa = 3.92QYY311 pKa = 11.38DD312 pKa = 3.96FGEE315 pKa = 4.51GSGLKK320 pKa = 10.18NVIRR324 pKa = 11.84FNLPEE329 pKa = 4.57GSSSGTHH336 pKa = 5.63LQSSAIPSTQAGPTTFFVVARR357 pKa = 11.84RR358 pKa = 11.84DD359 pKa = 3.43THH361 pKa = 6.4GFSSYY366 pKa = 11.01LFDD369 pKa = 5.93GISDD373 pKa = 3.59NARR376 pKa = 11.84RR377 pKa = 11.84GVSANGLTDD386 pKa = 2.98THH388 pKa = 6.99RR389 pKa = 11.84LQVPEE394 pKa = 4.13FDD396 pKa = 3.74SVGVDD401 pKa = 3.02VGVWQVFSAVFNEE414 pKa = 4.24EE415 pKa = 3.77FSQLFIDD422 pKa = 5.69GFTADD427 pKa = 3.83EE428 pKa = 4.43YY429 pKa = 11.62GFSGVDD435 pKa = 3.01AFEE438 pKa = 4.44GLTVGSRR445 pKa = 11.84YY446 pKa = 10.15SGSNTMDD453 pKa = 2.6GWIAEE458 pKa = 4.14LLVYY462 pKa = 10.47EE463 pKa = 4.89GALDD467 pKa = 3.93ADD469 pKa = 3.64SRR471 pKa = 11.84KK472 pKa = 9.99AVEE475 pKa = 3.7ASLMQKK481 pKa = 10.63YY482 pKa = 10.45NITPFCGASYY492 pKa = 8.78TQYY495 pKa = 11.56SPMDD499 pKa = 3.65FDD501 pKa = 5.21KK502 pKa = 11.52NCIVNFMDD510 pKa = 3.75YY511 pKa = 11.41AKK513 pKa = 10.53FGQSWLDD520 pKa = 3.46DD521 pKa = 3.77FASIGISSLSGIKK534 pKa = 9.95PSADD538 pKa = 3.3FAHH541 pKa = 6.49GWEE544 pKa = 4.01MDD546 pKa = 3.68YY547 pKa = 11.37YY548 pKa = 9.81PWDD551 pKa = 3.49VANGVDD557 pKa = 4.46EE558 pKa = 6.19DD559 pKa = 4.57NDD561 pKa = 4.12GQIDD565 pKa = 3.89WVRR568 pKa = 11.84SSSNHH573 pKa = 5.82FEE575 pKa = 4.17LSTEE579 pKa = 4.07GTLVNISDD587 pKa = 3.82VSGSLLANDD596 pKa = 5.31DD597 pKa = 3.74NWDD600 pKa = 3.53QAFWPQPDD608 pKa = 3.2INAATGFTTEE618 pKa = 4.24FSIKK622 pKa = 8.76ITSDD626 pKa = 2.86TGSNGAFKK634 pKa = 10.45IQVSPYY640 pKa = 10.45DD641 pKa = 3.5SVNTDD646 pKa = 3.03VVFIGASHH654 pKa = 4.98VTYY657 pKa = 10.84YY658 pKa = 11.18VDD660 pKa = 3.68GGDD663 pKa = 4.45NIVLDD668 pKa = 4.26TSDD671 pKa = 3.52NTDD674 pKa = 3.03GFHH677 pKa = 6.75SFRR680 pKa = 11.84IARR683 pKa = 11.84HH684 pKa = 4.61VDD686 pKa = 3.25EE687 pKa = 4.7PTKK690 pKa = 10.94SSVWKK695 pKa = 10.59DD696 pKa = 3.23GVLIGDD702 pKa = 4.31SLDD705 pKa = 3.88VTWTPSGPHH714 pKa = 6.68RR715 pKa = 11.84YY716 pKa = 9.75YY717 pKa = 10.33IGDD720 pKa = 3.2IDD722 pKa = 4.49SSVNGTYY729 pKa = 10.87EE730 pKa = 3.81MDD732 pKa = 3.95YY733 pKa = 11.37LRR735 pKa = 11.84IDD737 pKa = 3.68PGAWAAPAPMDD748 pKa = 4.02FDD750 pKa = 4.1EE751 pKa = 6.2DD752 pKa = 4.94GIVGSKK758 pKa = 10.02DD759 pKa = 3.37LKK761 pKa = 11.17SFALDD766 pKa = 3.37WLNDD770 pKa = 3.54TDD772 pKa = 4.29PANIEE777 pKa = 3.95

Molecular weight: 84.95 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1Q2MFI5|A0A1Q2MFI5_9BACT Ribulokinase OS=Limihaloglobus sulfuriphilus OX=1851148 GN=araB PE=3 SV=1
MM1 pKa = 7.56EE2 pKa = 5.76FDD4 pKa = 5.77KK5 pKa = 11.27ASQWLLKK12 pKa = 9.79SLRR15 pKa = 11.84RR16 pKa = 11.84MKK18 pKa = 10.49LLKK21 pKa = 10.32QLAQPMTAFQLYY33 pKa = 9.33KK34 pKa = 9.56RR35 pKa = 11.84TGISRR40 pKa = 11.84RR41 pKa = 11.84DD42 pKa = 3.54CSDD45 pKa = 3.76VIVEE49 pKa = 4.26MYY51 pKa = 10.47KK52 pKa = 10.71AGLLFCLNPKK62 pKa = 8.62ATRR65 pKa = 11.84SRR67 pKa = 11.84LFGLTKK73 pKa = 9.53TGKK76 pKa = 10.11SIRR79 pKa = 11.84KK80 pKa = 8.35QLFPRR85 pKa = 11.84IEE87 pKa = 4.29PYY89 pKa = 10.36YY90 pKa = 10.74EE91 pKa = 5.11PDD93 pKa = 3.43IDD95 pKa = 3.62WNAYY99 pKa = 10.04GYY101 pKa = 11.09VCFSQRR107 pKa = 11.84SAIILALDD115 pKa = 3.91SPRR118 pKa = 11.84SPAGIKK124 pKa = 9.95RR125 pKa = 11.84YY126 pKa = 9.31LRR128 pKa = 11.84HH129 pKa = 6.54ILPKK133 pKa = 10.19TRR135 pKa = 11.84ISANNIRR142 pKa = 11.84NNIPMLKK149 pKa = 10.06ARR151 pKa = 11.84DD152 pKa = 3.78IIRR155 pKa = 11.84TIDD158 pKa = 3.23TRR160 pKa = 11.84GVYY163 pKa = 9.67PEE165 pKa = 4.45YY166 pKa = 10.66EE167 pKa = 4.08LTVLGRR173 pKa = 11.84QLQTLLRR180 pKa = 11.84RR181 pKa = 11.84TASPSQQ187 pKa = 3.19

Molecular weight: 21.74 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2870

0

2870

1126502

30

3405

392.5

43.76

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.856 ± 0.044

1.437 ± 0.018

6.246 ± 0.037

6.363 ± 0.037

4.317 ± 0.031

7.59 ± 0.043

1.793 ± 0.016

6.878 ± 0.035

5.762 ± 0.051

8.655 ± 0.047

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.554 ± 0.023

4.546 ± 0.032

4.19 ± 0.026

3.252 ± 0.023

4.871 ± 0.038

6.718 ± 0.035

5.24 ± 0.037

6.475 ± 0.034

1.471 ± 0.019

3.785 ± 0.03
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski