Amino acid dipepetide frequency for Pectobacterium phage vB_PatM_CB7

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
6.483AlaAla: 6.483 ± 0.528
0.919AlaCys: 0.919 ± 0.133
3.961AlaAsp: 3.961 ± 0.336
4.904AlaGlu: 4.904 ± 0.338
2.593AlaPhe: 2.593 ± 0.226
5.847AlaGly: 5.847 ± 0.558
1.839AlaHis: 1.839 ± 0.188
4.031AlaIle: 4.031 ± 0.282
4.432AlaLys: 4.432 ± 0.355
5.092AlaLeu: 5.092 ± 0.351
2.263AlaMet: 2.263 ± 0.228
3.159AlaAsn: 3.159 ± 0.296
2.334AlaPro: 2.334 ± 0.262
2.994AlaGln: 2.994 ± 0.304
3.843AlaArg: 3.843 ± 0.353
3.348AlaSer: 3.348 ± 0.328
3.914AlaThr: 3.914 ± 0.472
4.998AlaVal: 4.998 ± 0.392
1.155AlaTrp: 1.155 ± 0.177
2.57AlaTyr: 2.57 ± 0.258
0.0AlaXaa: 0.0 ± 0.0
Cys
0.66CysAla: 0.66 ± 0.13
0.236CysCys: 0.236 ± 0.082
0.896CysAsp: 0.896 ± 0.134
1.037CysGlu: 1.037 ± 0.156
0.684CysPhe: 0.684 ± 0.14
1.556CysGly: 1.556 ± 0.181
0.495CysHis: 0.495 ± 0.123
0.283CysIle: 0.283 ± 0.076
0.707CysLys: 0.707 ± 0.124
1.037CysLeu: 1.037 ± 0.187
0.377CysMet: 0.377 ± 0.097
0.589CysAsn: 0.589 ± 0.11
0.754CysPro: 0.754 ± 0.147
0.448CysGln: 0.448 ± 0.11
0.872CysArg: 0.872 ± 0.137
0.66CysSer: 0.66 ± 0.131
0.495CysThr: 0.495 ± 0.13
0.542CysVal: 0.542 ± 0.1
0.118CysTrp: 0.118 ± 0.053
0.637CysTyr: 0.637 ± 0.114
0.0CysXaa: 0.0 ± 0.0
Asp
4.149AspAla: 4.149 ± 0.344
0.519AspCys: 0.519 ± 0.123
3.725AspAsp: 3.725 ± 0.356
3.937AspGlu: 3.937 ± 0.366
3.277AspPhe: 3.277 ± 0.236
5.399AspGly: 5.399 ± 0.487
1.674AspHis: 1.674 ± 0.2
3.961AspIle: 3.961 ± 0.33
3.819AspLys: 3.819 ± 0.248
6.342AspLeu: 6.342 ± 0.385
2.617AspMet: 2.617 ± 0.292
3.183AspAsn: 3.183 ± 0.307
3.065AspPro: 3.065 ± 0.251
2.381AspGln: 2.381 ± 0.196
3.112AspArg: 3.112 ± 0.295
3.277AspSer: 3.277 ± 0.293
3.206AspThr: 3.206 ± 0.397
4.173AspVal: 4.173 ± 0.331
1.367AspTrp: 1.367 ± 0.173
2.428AspTyr: 2.428 ± 0.212
0.0AspXaa: 0.0 ± 0.0
Glu
4.833GluAla: 4.833 ± 0.351
0.825GluCys: 0.825 ± 0.123
5.493GluAsp: 5.493 ± 0.405
5.705GluGlu: 5.705 ± 0.442
3.041GluPhe: 3.041 ± 0.248
5.422GluGly: 5.422 ± 0.316
1.65GluHis: 1.65 ± 0.203
4.55GluIle: 4.55 ± 0.371
4.715GluLys: 4.715 ± 0.364
5.281GluLeu: 5.281 ± 0.363
2.31GluMet: 2.31 ± 0.238
3.018GluAsn: 3.018 ± 0.256
1.58GluPro: 1.58 ± 0.205
2.405GluGln: 2.405 ± 0.244
3.536GluArg: 3.536 ± 0.293
3.041GluSer: 3.041 ± 0.242
3.395GluThr: 3.395 ± 0.277
5.705GluVal: 5.705 ± 0.357
1.155GluTrp: 1.155 ± 0.162
2.475GluTyr: 2.475 ± 0.262
0.0GluXaa: 0.0 ± 0.0
Phe
2.475PheAla: 2.475 ± 0.259
0.707PheCys: 0.707 ± 0.134
3.159PheAsp: 3.159 ± 0.288
2.853PheGlu: 2.853 ± 0.283
2.122PhePhe: 2.122 ± 0.227
3.56PheGly: 3.56 ± 0.361
0.66PheHis: 0.66 ± 0.119
2.523PheIle: 2.523 ± 0.215
2.853PheLys: 2.853 ± 0.296
3.348PheLeu: 3.348 ± 0.295
0.896PheMet: 0.896 ± 0.149
1.91PheAsn: 1.91 ± 0.242
1.485PhePro: 1.485 ± 0.184
1.179PheGln: 1.179 ± 0.153
1.674PheArg: 1.674 ± 0.182
2.405PheSer: 2.405 ± 0.235
2.428PheThr: 2.428 ± 0.288
2.853PheVal: 2.853 ± 0.262
0.896PheTrp: 0.896 ± 0.147
1.768PheTyr: 1.768 ± 0.203
0.0PheXaa: 0.0 ± 0.0
Gly
5.21GlyAla: 5.21 ± 0.587
1.132GlyCys: 1.132 ± 0.179
5.446GlyAsp: 5.446 ± 0.794
5.069GlyGlu: 5.069 ± 0.379
3.348GlyPhe: 3.348 ± 0.325
5.375GlyGly: 5.375 ± 0.364
1.179GlyHis: 1.179 ± 0.172
3.984GlyIle: 3.984 ± 0.326
7.026GlyLys: 7.026 ± 0.435
5.8GlyLeu: 5.8 ± 0.41
2.405GlyMet: 2.405 ± 0.252
3.324GlyAsn: 3.324 ± 0.34
2.664GlyPro: 2.664 ± 0.995
2.428GlyGln: 2.428 ± 0.274
3.961GlyArg: 3.961 ± 0.28
4.762GlySer: 4.762 ± 0.38
5.281GlyThr: 5.281 ± 0.596
5.682GlyVal: 5.682 ± 0.365
1.25GlyTrp: 1.25 ± 0.174
4.244GlyTyr: 4.244 ± 0.237
0.0GlyXaa: 0.0 ± 0.0
His
1.037HisAla: 1.037 ± 0.175
0.377HisCys: 0.377 ± 0.103
1.037HisAsp: 1.037 ± 0.184
1.485HisGlu: 1.485 ± 0.184
0.967HisPhe: 0.967 ± 0.166
1.532HisGly: 1.532 ± 0.176
0.377HisHis: 0.377 ± 0.092
1.32HisIle: 1.32 ± 0.198
1.344HisLys: 1.344 ± 0.176
1.674HisLeu: 1.674 ± 0.204
0.448HisMet: 0.448 ± 0.092
1.037HisAsn: 1.037 ± 0.183
1.179HisPro: 1.179 ± 0.165
0.495HisGln: 0.495 ± 0.101
1.745HisArg: 1.745 ± 0.173
0.967HisSer: 0.967 ± 0.143
1.179HisThr: 1.179 ± 0.172
1.438HisVal: 1.438 ± 0.177
0.448HisTrp: 0.448 ± 0.102
0.754HisTyr: 0.754 ± 0.149
0.0HisXaa: 0.0 ± 0.0
Ile
3.489IleAla: 3.489 ± 0.281
0.731IleCys: 0.731 ± 0.141
3.866IleAsp: 3.866 ± 0.251
4.244IleGlu: 4.244 ± 0.294
2.193IlePhe: 2.193 ± 0.246
3.843IleGly: 3.843 ± 0.348
1.202IleHis: 1.202 ± 0.184
2.499IleIle: 2.499 ± 0.274
3.489IleLys: 3.489 ± 0.313
3.937IleLeu: 3.937 ± 0.342
1.462IleMet: 1.462 ± 0.197
2.735IleAsn: 2.735 ± 0.259
2.452IlePro: 2.452 ± 0.274
1.745IleGln: 1.745 ± 0.187
4.031IleArg: 4.031 ± 0.248
3.159IleSer: 3.159 ± 0.282
3.301IleThr: 3.301 ± 0.281
3.749IleVal: 3.749 ± 0.329
0.825IleTrp: 0.825 ± 0.154
2.004IleTyr: 2.004 ± 0.218
0.0IleXaa: 0.0 ± 0.0
Lys
5.988LysAla: 5.988 ± 0.473
0.849LysCys: 0.849 ± 0.148
4.314LysAsp: 4.314 ± 0.335
4.81LysGlu: 4.81 ± 0.398
2.758LysPhe: 2.758 ± 0.251
5.894LysGly: 5.894 ± 0.587
1.32LysHis: 1.32 ± 0.236
3.749LysIle: 3.749 ± 0.311
4.574LysLys: 4.574 ± 0.357
4.456LysLeu: 4.456 ± 0.339
2.381LysMet: 2.381 ± 0.261
2.358LysAsn: 2.358 ± 0.25
2.617LysPro: 2.617 ± 0.272
2.334LysGln: 2.334 ± 0.26
3.701LysArg: 3.701 ± 0.3
3.819LysSer: 3.819 ± 0.308
3.631LysThr: 3.631 ± 0.281
4.644LysVal: 4.644 ± 0.295
1.108LysTrp: 1.108 ± 0.173
2.428LysTyr: 2.428 ± 0.247
0.0LysXaa: 0.0 ± 0.0
Leu
5.87LeuAla: 5.87 ± 0.392
1.132LeuCys: 1.132 ± 0.207
5.116LeuAsp: 5.116 ± 0.355
5.753LeuGlu: 5.753 ± 0.293
3.136LeuPhe: 3.136 ± 0.289
5.21LeuGly: 5.21 ± 0.354
1.721LeuHis: 1.721 ± 0.252
4.267LeuIle: 4.267 ± 0.275
5.281LeuLys: 5.281 ± 0.382
5.069LeuLeu: 5.069 ± 0.37
2.475LeuMet: 2.475 ± 0.252
3.843LeuAsn: 3.843 ± 0.272
3.725LeuPro: 3.725 ± 0.317
2.122LeuGln: 2.122 ± 0.221
4.149LeuArg: 4.149 ± 0.32
5.187LeuSer: 5.187 ± 0.323
4.479LeuThr: 4.479 ± 0.298
4.432LeuVal: 4.432 ± 0.358
1.155LeuTrp: 1.155 ± 0.165
2.641LeuTyr: 2.641 ± 0.228
0.0LeuXaa: 0.0 ± 0.0
Met
2.475MetAla: 2.475 ± 0.253
0.401MetCys: 0.401 ± 0.099
1.391MetAsp: 1.391 ± 0.2
2.287MetGlu: 2.287 ± 0.228
1.603MetPhe: 1.603 ± 0.23
1.721MetGly: 1.721 ± 0.182
0.448MetHis: 0.448 ± 0.117
2.004MetIle: 2.004 ± 0.206
2.617MetLys: 2.617 ± 0.233
1.957MetLeu: 1.957 ± 0.216
0.589MetMet: 0.589 ± 0.115
1.202MetAsn: 1.202 ± 0.15
0.99MetPro: 0.99 ± 0.155
0.872MetGln: 0.872 ± 0.139
1.367MetArg: 1.367 ± 0.203
2.122MetSer: 2.122 ± 0.222
1.745MetThr: 1.745 ± 0.186
2.098MetVal: 2.098 ± 0.217
0.33MetTrp: 0.33 ± 0.085
0.802MetTyr: 0.802 ± 0.127
0.0MetXaa: 0.0 ± 0.0
Asn
3.183AsnAla: 3.183 ± 0.249
0.542AsnCys: 0.542 ± 0.15
2.193AsnAsp: 2.193 ± 0.234
2.593AsnGlu: 2.593 ± 0.252
1.556AsnPhe: 1.556 ± 0.202
4.267AsnGly: 4.267 ± 0.303
0.825AsnHis: 0.825 ± 0.147
2.452AsnIle: 2.452 ± 0.263
2.711AsnLys: 2.711 ± 0.251
4.126AsnLeu: 4.126 ± 0.361
1.155AsnMet: 1.155 ± 0.182
2.287AsnAsn: 2.287 ± 0.271
2.098AsnPro: 2.098 ± 0.218
1.65AsnGln: 1.65 ± 0.204
2.641AsnArg: 2.641 ± 0.277
2.334AsnSer: 2.334 ± 0.218
2.711AsnThr: 2.711 ± 0.266
2.853AsnVal: 2.853 ± 0.234
0.637AsnTrp: 0.637 ± 0.11
1.532AsnTyr: 1.532 ± 0.166
0.0AsnXaa: 0.0 ± 0.0
Pro
2.758ProAla: 2.758 ± 0.37
0.566ProCys: 0.566 ± 0.103
2.9ProAsp: 2.9 ± 0.287
3.725ProGlu: 3.725 ± 0.322
1.532ProPhe: 1.532 ± 0.211
3.088ProGly: 3.088 ± 0.26
0.99ProHis: 0.99 ± 0.15
1.65ProIle: 1.65 ± 0.236
2.641ProLys: 2.641 ± 0.341
2.523ProLeu: 2.523 ± 0.288
1.037ProMet: 1.037 ± 0.163
1.532ProAsn: 1.532 ± 0.185
1.273ProPro: 1.273 ± 0.171
1.367ProGln: 1.367 ± 0.304
1.815ProArg: 1.815 ± 0.179
2.098ProSer: 2.098 ± 0.233
2.405ProThr: 2.405 ± 0.288
3.301ProVal: 3.301 ± 0.295
0.684ProTrp: 0.684 ± 0.145
1.415ProTyr: 1.415 ± 0.197
0.0ProXaa: 0.0 ± 0.0
Gln
2.475GlnAla: 2.475 ± 0.26
0.283GlnCys: 0.283 ± 0.075
1.768GlnAsp: 1.768 ± 0.23
2.358GlnGlu: 2.358 ± 0.267
1.391GlnPhe: 1.391 ± 0.17
2.688GlnGly: 2.688 ± 0.568
0.613GlnHis: 0.613 ± 0.128
2.452GlnIle: 2.452 ± 0.223
1.863GlnLys: 1.863 ± 0.21
2.593GlnLeu: 2.593 ± 0.22
0.99GlnMet: 0.99 ± 0.18
1.674GlnAsn: 1.674 ± 0.193
1.014GlnPro: 1.014 ± 0.159
1.32GlnGln: 1.32 ± 0.206
1.697GlnArg: 1.697 ± 0.237
1.509GlnSer: 1.509 ± 0.176
1.721GlnThr: 1.721 ± 0.223
2.806GlnVal: 2.806 ± 0.283
0.637GlnTrp: 0.637 ± 0.129
1.65GlnTyr: 1.65 ± 0.206
0.0GlnXaa: 0.0 ± 0.0
Arg
3.796ArgAla: 3.796 ± 0.34
0.825ArgCys: 0.825 ± 0.159
3.819ArgAsp: 3.819 ± 0.353
3.843ArgGlu: 3.843 ± 0.344
1.957ArgPhe: 1.957 ± 0.229
4.385ArgGly: 4.385 ± 0.298
0.967ArgHis: 0.967 ± 0.154
2.617ArgIle: 2.617 ± 0.283
4.385ArgLys: 4.385 ± 0.337
4.715ArgLeu: 4.715 ± 0.343
1.65ArgMet: 1.65 ± 0.19
2.075ArgAsn: 2.075 ± 0.216
1.933ArgPro: 1.933 ± 0.166
1.863ArgGln: 1.863 ± 0.222
3.112ArgArg: 3.112 ± 0.305
3.041ArgSer: 3.041 ± 0.277
2.216ArgThr: 2.216 ± 0.234
4.432ArgVal: 4.432 ± 0.328
0.849ArgTrp: 0.849 ± 0.155
1.839ArgTyr: 1.839 ± 0.199
0.0ArgXaa: 0.0 ± 0.0
Ser
3.725SerAla: 3.725 ± 0.307
0.778SerCys: 0.778 ± 0.13
4.126SerAsp: 4.126 ± 0.301
3.253SerGlu: 3.253 ± 0.267
2.028SerPhe: 2.028 ± 0.215
4.998SerGly: 4.998 ± 0.32
0.99SerHis: 0.99 ± 0.135
2.475SerIle: 2.475 ± 0.227
3.654SerLys: 3.654 ± 0.318
4.644SerLeu: 4.644 ± 0.352
1.32SerMet: 1.32 ± 0.144
2.145SerAsn: 2.145 ± 0.216
2.546SerPro: 2.546 ± 0.253
1.886SerGln: 1.886 ± 0.2
2.994SerArg: 2.994 ± 0.312
3.937SerSer: 3.937 ± 0.515
3.112SerThr: 3.112 ± 0.351
4.409SerVal: 4.409 ± 0.365
1.014SerTrp: 1.014 ± 0.164
1.957SerTyr: 1.957 ± 0.284
0.0SerXaa: 0.0 ± 0.0
Thr
3.206ThrAla: 3.206 ± 0.3
0.613ThrCys: 0.613 ± 0.119
3.253ThrAsp: 3.253 ± 0.296
3.206ThrGlu: 3.206 ± 0.271
2.405ThrPhe: 2.405 ± 0.241
6.153ThrGly: 6.153 ± 0.967
1.179ThrHis: 1.179 ± 0.184
3.277ThrIle: 3.277 ± 0.31
3.489ThrLys: 3.489 ± 0.249
4.597ThrLeu: 4.597 ± 0.339
1.32ThrMet: 1.32 ± 0.175
2.334ThrAsn: 2.334 ± 0.22
2.971ThrPro: 2.971 ± 0.27
1.65ThrGln: 1.65 ± 0.209
2.971ThrArg: 2.971 ± 0.259
2.758ThrSer: 2.758 ± 0.278
3.065ThrThr: 3.065 ± 0.409
4.621ThrVal: 4.621 ± 0.416
0.849ThrTrp: 0.849 ± 0.15
1.839ThrTyr: 1.839 ± 0.197
0.0ThrXaa: 0.0 ± 0.0
Val
5.47ValAla: 5.47 ± 0.341
0.778ValCys: 0.778 ± 0.174
5.305ValAsp: 5.305 ± 0.327
5.588ValGlu: 5.588 ± 0.375
2.947ValPhe: 2.947 ± 0.279
4.668ValGly: 4.668 ± 0.298
1.273ValHis: 1.273 ± 0.185
4.338ValIle: 4.338 ± 0.27
5.022ValLys: 5.022 ± 0.316
4.668ValLeu: 4.668 ± 0.374
1.933ValMet: 1.933 ± 0.225
3.654ValAsn: 3.654 ± 0.343
2.546ValPro: 2.546 ± 0.247
2.334ValGln: 2.334 ± 0.253
3.772ValArg: 3.772 ± 0.329
4.385ValSer: 4.385 ± 0.359
4.621ValThr: 4.621 ± 0.339
5.328ValVal: 5.328 ± 0.384
1.179ValTrp: 1.179 ± 0.185
2.664ValTyr: 2.664 ± 0.245
0.0ValXaa: 0.0 ± 0.0
Trp
1.273TrpAla: 1.273 ± 0.201
0.165TrpCys: 0.165 ± 0.065
1.344TrpAsp: 1.344 ± 0.184
1.179TrpGlu: 1.179 ± 0.152
0.731TrpPhe: 0.731 ± 0.126
0.967TrpGly: 0.967 ± 0.124
0.401TrpHis: 0.401 ± 0.098
0.872TrpIle: 0.872 ± 0.113
1.179TrpLys: 1.179 ± 0.178
1.108TrpLeu: 1.108 ± 0.167
0.542TrpMet: 0.542 ± 0.111
0.589TrpAsn: 0.589 ± 0.142
0.519TrpPro: 0.519 ± 0.104
0.613TrpGln: 0.613 ± 0.114
0.99TrpArg: 0.99 ± 0.143
0.99TrpSer: 0.99 ± 0.165
0.849TrpThr: 0.849 ± 0.143
1.202TrpVal: 1.202 ± 0.152
0.259TrpTrp: 0.259 ± 0.073
0.707TrpTyr: 0.707 ± 0.144
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.122TyrAla: 2.122 ± 0.213
0.707TyrCys: 0.707 ± 0.131
2.499TyrAsp: 2.499 ± 0.225
2.31TyrGlu: 2.31 ± 0.194
1.485TyrPhe: 1.485 ± 0.19
3.018TyrGly: 3.018 ± 0.276
1.037TyrHis: 1.037 ± 0.16
1.65TyrIle: 1.65 ± 0.201
1.933TyrLys: 1.933 ± 0.212
3.796TyrLeu: 3.796 ± 0.304
0.825TyrMet: 0.825 ± 0.14
1.697TyrAsn: 1.697 ± 0.181
1.556TyrPro: 1.556 ± 0.183
1.415TyrGln: 1.415 ± 0.154
2.334TyrArg: 2.334 ± 0.291
2.193TyrSer: 2.193 ± 0.264
2.004TyrThr: 2.004 ± 0.24
3.206TyrVal: 3.206 ± 0.257
0.566TyrTrp: 0.566 ± 0.114
1.32TyrTyr: 1.32 ± 0.183
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 253 proteins (42417 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski