Microbacterium phage Rhysand

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; unclassified Siphoviridae

Average proteome isoelectric point is 6.46

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 25 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A4D6E1I9|A0A4D6E1I9_9CAUD Minor tail protein OS=Microbacterium phage Rhysand OX=2562190 GN=7 PE=4 SV=1
MM1 pKa = 7.26TLIEE5 pKa = 5.13DD6 pKa = 3.87VLSEE10 pKa = 4.01QIAALRR16 pKa = 11.84ASAGLFVRR24 pKa = 11.84QRR26 pKa = 11.84ADD28 pKa = 2.85ALAAAEE34 pKa = 4.04AAAVQAAGADD44 pKa = 3.39ARR46 pKa = 11.84AAALQAEE53 pKa = 4.52LDD55 pKa = 3.79AYY57 pKa = 10.91RR58 pKa = 11.84EE59 pKa = 4.02LLPAPEE65 pKa = 4.43IPEE68 pKa = 4.26DD69 pKa = 3.87APAVEE74 pKa = 4.85LPADD78 pKa = 3.77EE79 pKa = 4.57TTPPLYY85 pKa = 10.19QQLLTEE91 pKa = 4.46RR92 pKa = 11.84EE93 pKa = 4.51GNN95 pKa = 3.52

Molecular weight: 9.97 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A4D6E393|A0A4D6E393_9CAUD Uncharacterized protein OS=Microbacterium phage Rhysand OX=2562190 GN=19 PE=4 SV=1
MM1 pKa = 7.94AEE3 pKa = 3.9YY4 pKa = 10.13QNRR7 pKa = 11.84GIRR10 pKa = 11.84IDD12 pKa = 3.73GLRR15 pKa = 11.84EE16 pKa = 3.64LNAKK20 pKa = 9.43LRR22 pKa = 11.84AAGDD26 pKa = 3.72EE27 pKa = 4.39SADD30 pKa = 4.17LPDD33 pKa = 3.73LMFRR37 pKa = 11.84LGSIVIANARR47 pKa = 11.84VPAKK51 pKa = 10.34SGEE54 pKa = 4.09LAGTLRR60 pKa = 11.84AGRR63 pKa = 11.84GRR65 pKa = 11.84TKK67 pKa = 10.34AVVRR71 pKa = 11.84AGYY74 pKa = 10.43AKK76 pKa = 10.18RR77 pKa = 11.84GAHH80 pKa = 6.33AGVVHH85 pKa = 6.46YY86 pKa = 9.46GNPHH90 pKa = 5.42TGSRR94 pKa = 11.84AQPFLVDD101 pKa = 3.69ALRR104 pKa = 11.84RR105 pKa = 11.84AQSQLVSEE113 pKa = 4.67LQTGIDD119 pKa = 3.26QLMRR123 pKa = 11.84KK124 pKa = 9.03HH125 pKa = 6.36KK126 pKa = 10.6LL127 pKa = 3.21

Molecular weight: 13.75 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

25

0

25

5522

70

701

220.9

23.65

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

13.999 ± 0.642

0.344 ± 0.159

5.487 ± 0.542

5.433 ± 0.493

3.024 ± 0.235

9.055 ± 0.665

1.485 ± 0.217

4.509 ± 0.39

1.92 ± 0.257

9.507 ± 0.806

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.992 ± 0.243

2.988 ± 0.356

5.089 ± 0.352

4.201 ± 0.31

7.443 ± 0.754

5.813 ± 0.337

6.501 ± 0.499

7.588 ± 0.576

1.431 ± 0.189

2.191 ± 0.244
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski