Acidovorax sp.
Average proteome isoelectric point is 6.92
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 5416 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A519LSR5|A0A519LSR5_9BURK M23 family metallopeptidase OS=Acidovorax sp. OX=1872122 GN=EON49_24040 PE=4 SV=1
MM1 pKa = 7.42 SLNYY5 pKa = 10.28 LDD7 pKa = 5.18 FDD9 pKa = 4.26 YY10 pKa = 11.66 SEE12 pKa = 5.41 DD13 pKa = 4.23 DD14 pKa = 3.65 EE15 pKa = 4.88 GTGTFDD21 pKa = 4.43 AMASVLPAQLAALHH35 pKa = 6.63 AEE37 pKa = 4.54 VAQVLQWAHH46 pKa = 6.26 SEE48 pKa = 4.27 FPDD51 pKa = 3.48 ACGPSEE57 pKa = 6.53 DD58 pKa = 4.12 GGQWQYY64 pKa = 12.08 DD65 pKa = 3.73 LQGVQDD71 pKa = 3.77 VSTSLVLDD79 pKa = 4.11 FDD81 pKa = 4.32 AATGSIHH88 pKa = 6.58 SAPGTPAPPRR98 pKa = 11.84 TTLTLSVSGSKK109 pKa = 10.37 DD110 pKa = 2.91 FCAALRR116 pKa = 11.84 NAFGISS122 pKa = 3.12
Molecular weight: 12.84 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.713
IPC2_protein 3.783
IPC_protein 3.757
Toseland 3.541
ProMoST 3.948
Dawson 3.77
Bjellqvist 3.923
Wikipedia 3.732
Rodwell 3.579
Grimsley 3.452
Solomon 3.745
Lehninger 3.706
Nozaki 3.897
DTASelect 4.139
Thurlkill 3.617
EMBOSS 3.745
Sillero 3.884
Patrickios 0.846
IPC_peptide 3.745
IPC2_peptide 3.846
IPC2.peptide.svr19 3.778
Protein with the highest isoelectric point:
>tr|A0A519MI72|A0A519MI72_9BURK UPF0060 membrane protein EON49_05900 OS=Acidovorax sp. OX=1872122 GN=EON49_05900 PE=3 SV=1
MM1 pKa = 6.73 QQLLARR7 pKa = 11.84 IGRR10 pKa = 11.84 WPGLRR15 pKa = 11.84 WIEE18 pKa = 3.83 GWVRR22 pKa = 11.84 SLPPWAAVALFALPTALLLPVKK44 pKa = 10.37 LLALWAMGRR53 pKa = 11.84 GHH55 pKa = 6.58 VVLGMLVIALAKK67 pKa = 10.12 VVGTAVVARR76 pKa = 11.84 LFTLTQPALMQLRR89 pKa = 11.84 WFAWAYY95 pKa = 5.37 THH97 pKa = 7.39 WISFKK102 pKa = 10.88 HH103 pKa = 5.46 RR104 pKa = 11.84 VLKK107 pKa = 10.34 RR108 pKa = 11.84 ARR110 pKa = 11.84 ASWAWRR116 pKa = 11.84 VARR119 pKa = 11.84 VVRR122 pKa = 11.84 RR123 pKa = 11.84 HH124 pKa = 4.29 WVQRR128 pKa = 11.84 WRR130 pKa = 11.84 RR131 pKa = 11.84 RR132 pKa = 3.39
Molecular weight: 15.47 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.442
IPC2_protein 11.082
IPC_protein 12.544
Toseland 12.705
ProMoST 13.203
Dawson 12.705
Bjellqvist 12.705
Wikipedia 13.188
Rodwell 12.296
Grimsley 12.749
Solomon 13.203
Lehninger 13.1
Nozaki 12.705
DTASelect 12.705
Thurlkill 12.705
EMBOSS 13.203
Sillero 12.705
Patrickios 12.018
IPC_peptide 13.203
IPC2_peptide 12.193
IPC2.peptide.svr19 9.121
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
5416
0
5416
1423572
21
3428
262.8
28.37
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
13.44 ± 0.055
0.881 ± 0.014
5.182 ± 0.03
4.845 ± 0.033
3.351 ± 0.025
8.426 ± 0.032
2.374 ± 0.019
4.042 ± 0.026
3.073 ± 0.036
10.641 ± 0.047
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.431 ± 0.016
2.535 ± 0.024
5.383 ± 0.031
4.337 ± 0.022
6.65 ± 0.031
5.375 ± 0.029
5.506 ± 0.035
7.851 ± 0.028
1.538 ± 0.016
2.138 ± 0.018
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here