Cellulomonas aerilata
Average proteome isoelectric point is 6.21
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 3577 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A512D9F0|A0A512D9F0_9CELL Probable DNA ligase OS=Cellulomonas aerilata OX=515326 GN=lig_1 PE=3 SV=1
MM1 pKa = 6.69 ITKK4 pKa = 9.65 RR5 pKa = 11.84 WSYY8 pKa = 9.12 ATAVAAATAVVLAGCSASSSSEE30 pKa = 3.52 PSASDD35 pKa = 3.79 GPVTLTYY42 pKa = 10.27 WDD44 pKa = 4.61 FLDD47 pKa = 4.78 PSQDD51 pKa = 3.46 NPRR54 pKa = 11.84 SNALEE59 pKa = 3.77 EE60 pKa = 4.57 NIANFEE66 pKa = 4.08 AANPDD71 pKa = 2.96 ITIDD75 pKa = 3.66 LSVVSLGDD83 pKa = 3.26 MLNRR87 pKa = 11.84 LPQSAAAGQAPDD99 pKa = 3.63 VFKK102 pKa = 10.68 MFTPTVPQMAAAGAYY117 pKa = 10.09 SPLPEE122 pKa = 4.63 AASEE126 pKa = 3.99 ITDD129 pKa = 3.51 WLRR132 pKa = 11.84 PPSNLVGPDD141 pKa = 3.59 GQQVAVPYY149 pKa = 9.85 EE150 pKa = 3.84 YY151 pKa = 9.14 RR152 pKa = 11.84 TCAFYY157 pKa = 11.34 YY158 pKa = 8.85 NQKK161 pKa = 9.92 ILDD164 pKa = 3.83 QIGAEE169 pKa = 4.2 VPTTYY174 pKa = 11.14 EE175 pKa = 3.99 DD176 pKa = 3.77 VVDD179 pKa = 4.03 VAGKK183 pKa = 10.36 AGAAGFTGFGTGFSDD198 pKa = 3.65 TDD200 pKa = 3.16 NSAIISTFFDD210 pKa = 3.48 CFMSQVGEE218 pKa = 4.65 PIWNDD223 pKa = 3.11 DD224 pKa = 3.84 GEE226 pKa = 4.93 AEE228 pKa = 4.3 FAGTAADD235 pKa = 3.67 EE236 pKa = 4.6 FGQILADD243 pKa = 3.79 LRR245 pKa = 11.84 DD246 pKa = 3.73 SGGLGTSVVSDD257 pKa = 3.73 SYY259 pKa = 11.37 STVTDD264 pKa = 3.59 GLTNGTVAMAVLGSEE279 pKa = 4.38 RR280 pKa = 11.84 VITLTGANPDD290 pKa = 3.95 IKK292 pKa = 9.95 WGPLPEE298 pKa = 4.34 PTSGGTTGATLGWTLGIGAGTDD320 pKa = 3.23 HH321 pKa = 7.49 TDD323 pKa = 3.4 AAWKK327 pKa = 9.79 FIEE330 pKa = 4.34 YY331 pKa = 7.18 MTGAEE336 pKa = 4.42 AGAVMATGGEE346 pKa = 4.15 IPTRR350 pKa = 11.84 ASTYY354 pKa = 8.71 DD355 pKa = 3.09 AEE357 pKa = 4.52 YY358 pKa = 10.48 FSTPEE363 pKa = 3.85 AEE365 pKa = 4.44 TVKK368 pKa = 10.16 TVADD372 pKa = 3.77 YY373 pKa = 11.53 VEE375 pKa = 5.2 SNSEE379 pKa = 3.66 PHH381 pKa = 6.54 TYY383 pKa = 10.44 SDD385 pKa = 3.42 DD386 pKa = 4.1 WIGVASGLSAAGQSLYY402 pKa = 11.17 LNKK405 pKa = 10.14 TSGSEE410 pKa = 4.38 FITAAQDD417 pKa = 4.16 AINEE421 pKa = 4.19
Molecular weight: 43.89 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.692
IPC2_protein 3.745
IPC_protein 3.757
Toseland 3.541
ProMoST 3.897
Dawson 3.745
Bjellqvist 3.923
Wikipedia 3.668
Rodwell 3.579
Grimsley 3.452
Solomon 3.745
Lehninger 3.694
Nozaki 3.859
DTASelect 4.075
Thurlkill 3.592
EMBOSS 3.681
Sillero 3.884
Patrickios 1.252
IPC_peptide 3.732
IPC2_peptide 3.859
IPC2.peptide.svr19 3.796
Protein with the highest isoelectric point:
>tr|A0A512DFF7|A0A512DFF7_9CELL Cytidylate kinase OS=Cellulomonas aerilata OX=515326 GN=cmk PE=3 SV=1
MM1 pKa = 7.67 SKK3 pKa = 10.15 PAQLVLGVVLIVMGAVFTLQGLGVLAGSPMTGATLWAVVGPVLVVVGLVMVVRR56 pKa = 11.84 AARR59 pKa = 11.84 TRR61 pKa = 11.84 GPGRR65 pKa = 11.84 PRR67 pKa = 3.59
Molecular weight: 6.79 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.394
IPC2_protein 10.833
IPC_protein 12.442
Toseland 12.603
ProMoST 13.115
Dawson 12.603
Bjellqvist 12.603
Wikipedia 13.086
Rodwell 12.193
Grimsley 12.647
Solomon 13.1
Lehninger 12.998
Nozaki 12.603
DTASelect 12.603
Thurlkill 12.603
EMBOSS 13.1
Sillero 12.603
Patrickios 11.974
IPC_peptide 13.1
IPC2_peptide 12.091
IPC2.peptide.svr19 9.137
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
3577
0
3577
1211180
39
2028
338.6
35.77
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
14.545 ± 0.069
0.556 ± 0.009
6.474 ± 0.039
5.092 ± 0.037
2.424 ± 0.024
9.749 ± 0.041
2.135 ± 0.022
2.687 ± 0.03
1.217 ± 0.024
10.294 ± 0.05
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
1.571 ± 0.014
1.402 ± 0.024
6.312 ± 0.041
2.59 ± 0.022
8.135 ± 0.036
4.963 ± 0.029
6.405 ± 0.036
10.246 ± 0.044
1.46 ± 0.016
1.743 ± 0.021
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here