Tortoise microvirus 40

Taxonomy: Viruses; Monodnaviria; Sangervirae; Phixviricota; Malgrandaviricetes; Petitvirales; Microviridae; unclassified Microviridae

Average proteome isoelectric point is 6.07

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A4P8W6A4|A0A4P8W6A4_9VIRU Major capsid protein OS=Tortoise microvirus 40 OX=2583144 PE=3 SV=1
MM1 pKa = 7.12FSKK4 pKa = 10.54RR5 pKa = 11.84RR6 pKa = 11.84TTPYY10 pKa = 10.54VYY12 pKa = 10.78VDD14 pKa = 3.93DD15 pKa = 4.4PVIVSSKK22 pKa = 10.88EE23 pKa = 3.93MICSEE28 pKa = 4.44FKK30 pKa = 11.03EE31 pKa = 4.33EE32 pKa = 4.23TPVDD36 pKa = 3.39QFLQQEE42 pKa = 4.29IEE44 pKa = 4.37VNGVKK49 pKa = 10.34SIRR52 pKa = 11.84LTSDD56 pKa = 1.95IYY58 pKa = 11.07MLFNQQRR65 pKa = 11.84LDD67 pKa = 3.49RR68 pKa = 11.84LSRR71 pKa = 11.84EE72 pKa = 3.74SLLAYY77 pKa = 10.34FEE79 pKa = 4.99SISVNEE85 pKa = 4.29PKK87 pKa = 10.74FGDD90 pKa = 3.72LRR92 pKa = 11.84SKK94 pKa = 11.05LGDD97 pKa = 3.57DD98 pKa = 3.51QLISFVKK105 pKa = 10.43SRR107 pKa = 11.84FIQSPSEE114 pKa = 3.7LMAWSSYY121 pKa = 10.62LMSLMSSSDD130 pKa = 3.15AAIAEE135 pKa = 4.32LSALQSQVQPEE146 pKa = 4.52AQPEE150 pKa = 4.17AQPEE154 pKa = 4.12AQPP157 pKa = 3.74

Molecular weight: 17.89 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A4P8W641|A0A4P8W641_9VIRU Internal scaffolding protein OS=Tortoise microvirus 40 OX=2583144 PE=4 SV=1
MM1 pKa = 7.87AIGATAASLIAAGVGAVGSTAGAVLSNKK29 pKa = 8.18GTASIANNNNNFSEE43 pKa = 4.28KK44 pKa = 10.21MLDD47 pKa = 3.44KK48 pKa = 10.9QIAYY52 pKa = 9.63NKK54 pKa = 10.07EE55 pKa = 3.96MYY57 pKa = 9.3QQQLGDD63 pKa = 3.04QWQFYY68 pKa = 11.14NDD70 pKa = 3.43AKK72 pKa = 10.52QNAWDD77 pKa = 3.59MAEE80 pKa = 4.29YY81 pKa = 10.8NSAPAQRR88 pKa = 11.84EE89 pKa = 3.96RR90 pKa = 11.84LEE92 pKa = 4.2AAGLNPYY99 pKa = 11.24LMMNGGSVGTAQSSAQGSAPSAQGVNPPTATPYY132 pKa = 11.35SMDD135 pKa = 3.13YY136 pKa = 10.75SGLVQGLGMALDD148 pKa = 4.86QISKK152 pKa = 9.58MPDD155 pKa = 2.91NSVKK159 pKa = 10.5RR160 pKa = 11.84AEE162 pKa = 4.27ADD164 pKa = 3.14NLRR167 pKa = 11.84IEE169 pKa = 4.8GKK171 pKa = 9.98YY172 pKa = 9.72KK173 pKa = 9.12AAKK176 pKa = 8.41MVAEE180 pKa = 4.63IAQMRR185 pKa = 11.84TNAHH189 pKa = 4.54TQKK192 pKa = 10.7GRR194 pKa = 11.84LALDD198 pKa = 3.27KK199 pKa = 10.8LIYY202 pKa = 10.82SIDD205 pKa = 3.82KK206 pKa = 10.4DD207 pKa = 3.86LKK209 pKa = 8.11TSQMSVNSEE218 pKa = 4.28NIANMQAQRR227 pKa = 11.84KK228 pKa = 8.28LINVQTLFAEE238 pKa = 4.78KK239 pKa = 10.05QLSWMDD245 pKa = 3.56AQNKK249 pKa = 8.51MDD251 pKa = 5.14LAQKK255 pKa = 8.87TADD258 pKa = 3.4IQLKK262 pKa = 8.49YY263 pKa = 10.67AQGALTKK270 pKa = 10.2KK271 pKa = 9.88QVEE274 pKa = 4.29HH275 pKa = 6.4EE276 pKa = 4.37VNKK279 pKa = 10.26IAEE282 pKa = 4.27TAARR286 pKa = 11.84TSLIGVQKK294 pKa = 10.49QGQSLQNQFDD304 pKa = 3.79SATFGNRR311 pKa = 11.84VKK313 pKa = 9.82TVKK316 pKa = 9.55ATLYY320 pKa = 10.92NLISDD325 pKa = 4.79AYY327 pKa = 9.6SLPKK331 pKa = 10.35AAGSYY336 pKa = 10.38FMNTHH341 pKa = 4.83IQKK344 pKa = 10.2EE345 pKa = 4.3FFKK348 pKa = 11.15

Molecular weight: 37.86 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4

0

4

1582

157

580

395.5

44.91

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.143 ± 1.916

1.517 ± 0.724

6.384 ± 0.556

4.551 ± 0.982

6.131 ± 1.184

4.867 ± 0.882

1.58 ± 0.605

4.298 ± 0.278

5.942 ± 0.788

8.534 ± 0.298

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.592 ± 0.694

6.384 ± 1.369

3.666 ± 0.441

5.563 ± 1.168

3.982 ± 0.629

9.735 ± 0.931

4.741 ± 0.371

5.879 ± 0.465

0.948 ± 0.114

5.563 ± 0.866
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski