Escherichia phage mEp460_ev081

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; unclassified Siphoviridae

Average proteome isoelectric point is 6.76

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 60 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A653FW79|A0A653FW79_9CAUD GnsB protein OS=Escherichia phage mEp460_ev081 OX=2695846 PE=4 SV=1
MM1 pKa = 7.39PVEE4 pKa = 4.26ILSMDD9 pKa = 4.77FIASPPHH16 pKa = 5.29GTDD19 pKa = 4.45GIVIMCRR26 pKa = 11.84LPDD29 pKa = 3.44TSIRR33 pKa = 11.84ALIDD37 pKa = 3.28IYY39 pKa = 11.44AGDD42 pKa = 3.86DD43 pKa = 3.91AII45 pKa = 6.29

Molecular weight: 4.84 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A653FWX1|A0A653FWX1_9CAUD Uncharacterized protein OS=Escherichia phage mEp460_ev081 OX=2695846 PE=4 SV=1
MM1 pKa = 7.57TDD3 pKa = 4.1EE4 pKa = 4.16ISKK7 pKa = 9.61TSLFPRR13 pKa = 11.84RR14 pKa = 11.84SRR16 pKa = 11.84PVAGTPTGRR25 pKa = 11.84TRR27 pKa = 11.84QPDD30 pKa = 3.32NRR32 pKa = 11.84DD33 pKa = 2.98EE34 pKa = 4.2HH35 pKa = 6.89LKK37 pKa = 10.78QSVTT41 pKa = 3.52

Molecular weight: 4.67 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

60

0

60

12989

38

1137

216.5

23.98

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.939 ± 0.855

1.163 ± 0.194

5.774 ± 0.272

6.506 ± 0.325

3.249 ± 0.245

7.083 ± 0.277

1.725 ± 0.195

5.258 ± 0.26

5.528 ± 0.247

7.375 ± 0.483

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.595 ± 0.217

4.265 ± 0.251

4.065 ± 0.34

4.458 ± 0.47

6.313 ± 0.46

7.075 ± 0.567

6.459 ± 0.518

6.706 ± 0.429

1.694 ± 0.154

2.772 ± 0.155
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski