Amino acid dipepetide frequency for Firmicutes bacterium CAG:95

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	6.77AlaAla: 6.77 ± 0.106	1.048AlaCys: 1.048 ± 0.037	4.47AlaAsp: 4.47 ± 0.08	5.336AlaGlu: 5.336 ± 0.087	3.024AlaPhe: 3.024 ± 0.062	5.971AlaGly: 5.971 ± 0.091	1.104AlaHis: 1.104 ± 0.039	4.965AlaIle: 4.965 ± 0.074	4.29AlaLys: 4.29 ± 0.077	6.685AlaLeu: 6.685 ± 0.089	2.268AlaMet: 2.268 ± 0.062	2.399AlaAsn: 2.399 ± 0.061	1.983AlaPro: 1.983 ± 0.058	2.427AlaGln: 2.427 ± 0.061	2.845AlaArg: 2.845 ± 0.056	3.929AlaSer: 3.929 ± 0.07	3.097AlaThr: 3.097 ± 0.07	6.099AlaVal: 6.099 ± 0.096	0.577AlaTrp: 0.577 ± 0.025	2.924AlaTyr: 2.924 ± 0.064	0.001AlaXaa: 0.001 ± 0.001
Cys	1.033CysAla: 1.033 ± 0.036	0.3CysCys: 0.3 ± 0.019	0.801CysAsp: 0.801 ± 0.029	1.066CysGlu: 1.066 ± 0.036	0.766CysPhe: 0.766 ± 0.031	1.45CysGly: 1.45 ± 0.051	0.343CysHis: 0.343 ± 0.018	1.086CysIle: 1.086 ± 0.037	0.783CysLys: 0.783 ± 0.028	1.258CysLeu: 1.258 ± 0.042	0.505CysMet: 0.505 ± 0.023	0.651CysAsn: 0.651 ± 0.028	0.591CysPro: 0.591 ± 0.032	0.377CysGln: 0.377 ± 0.023	0.75CysArg: 0.75 ± 0.03	0.836CysSer: 0.836 ± 0.034	0.706CysThr: 0.706 ± 0.027	1.081CysVal: 1.081 ± 0.036	0.132CysTrp: 0.132 ± 0.012	0.644CysTyr: 0.644 ± 0.03	0.0CysXaa: 0.0 ± 0.0
Asp	4.172AspAla: 4.172 ± 0.077	0.782AspCys: 0.782 ± 0.033	2.723AspAsp: 2.723 ± 0.061	4.569AspGlu: 4.569 ± 0.078	2.63AspPhe: 2.63 ± 0.064	4.287AspGly: 4.287 ± 0.091	1.042AspHis: 1.042 ± 0.037	4.212AspIle: 4.212 ± 0.072	3.226AspLys: 3.226 ± 0.062	4.769AspLeu: 4.769 ± 0.071	1.817AspMet: 1.817 ± 0.045	2.208AspAsn: 2.208 ± 0.052	1.902AspPro: 1.902 ± 0.052	1.625AspGln: 1.625 ± 0.044	2.697AspArg: 2.697 ± 0.064	3.039AspSer: 3.039 ± 0.068	3.25AspThr: 3.25 ± 0.069	3.806AspVal: 3.806 ± 0.069	0.616AspTrp: 0.616 ± 0.03	2.907AspTyr: 2.907 ± 0.067	0.0AspXaa: 0.0 ± 0.0
Glu	5.939GluAla: 5.939 ± 0.103	0.853GluCys: 0.853 ± 0.036	4.654GluAsp: 4.654 ± 0.076	8.055GluGlu: 8.055 ± 0.134	2.637GluPhe: 2.637 ± 0.056	4.559GluGly: 4.559 ± 0.079	1.414GluHis: 1.414 ± 0.045	5.78GluIle: 5.78 ± 0.086	6.341GluLys: 6.341 ± 0.1	7.21GluLeu: 7.21 ± 0.099	2.49GluMet: 2.49 ± 0.05	4.154GluAsn: 4.154 ± 0.077	1.949GluPro: 1.949 ± 0.047	3.367GluGln: 3.367 ± 0.071	3.418GluArg: 3.418 ± 0.075	3.383GluSer: 3.383 ± 0.068	3.738GluThr: 3.738 ± 0.07	4.634GluVal: 4.634 ± 0.085	0.752GluTrp: 0.752 ± 0.031	3.313GluTyr: 3.313 ± 0.069	0.002GluXaa: 0.002 ± 0.002
Phe	2.957PheAla: 2.957 ± 0.065	0.771PheCys: 0.771 ± 0.034	2.581PheAsp: 2.581 ± 0.054	2.776PheGlu: 2.776 ± 0.061	1.939PhePhe: 1.939 ± 0.049	2.968PheGly: 2.968 ± 0.059	0.916PheHis: 0.916 ± 0.033	2.791PheIle: 2.791 ± 0.058	1.933PheLys: 1.933 ± 0.052	4.084PheLeu: 4.084 ± 0.076	1.259PheMet: 1.259 ± 0.042	1.427PheAsn: 1.427 ± 0.044	1.472PhePro: 1.472 ± 0.039	1.342PheGln: 1.342 ± 0.04	1.799PheArg: 1.799 ± 0.042	2.626PheSer: 2.626 ± 0.06	2.412PheThr: 2.412 ± 0.053	2.834PheVal: 2.834 ± 0.066	0.465PheTrp: 0.465 ± 0.025	1.95PheTyr: 1.95 ± 0.061	0.0PheXaa: 0.0 ± 0.0
Gly	4.705GlyAla: 4.705 ± 0.095	1.244GlyCys: 1.244 ± 0.038	3.475GlyAsp: 3.475 ± 0.066	4.939GlyGlu: 4.939 ± 0.078	3.117GlyPhe: 3.117 ± 0.062	4.389GlyGly: 4.389 ± 0.092	1.208GlyHis: 1.208 ± 0.036	6.247GlyIle: 6.247 ± 0.086	5.192GlyLys: 5.192 ± 0.082	5.645GlyLeu: 5.645 ± 0.075	2.658GlyMet: 2.658 ± 0.065	3.245GlyAsn: 3.245 ± 0.069	1.222GlyPro: 1.222 ± 0.041	2.13GlyGln: 2.13 ± 0.056	3.047GlyArg: 3.047 ± 0.064	4.019GlySer: 4.019 ± 0.081	4.13GlyThr: 4.13 ± 0.087	4.773GlyVal: 4.773 ± 0.085	0.697GlyTrp: 0.697 ± 0.032	3.246GlyTyr: 3.246 ± 0.068	0.001GlyXaa: 0.001 ± 0.001
His	1.161HisAla: 1.161 ± 0.038	0.303HisCys: 0.303 ± 0.022	0.91HisAsp: 0.91 ± 0.038	1.164HisGlu: 1.164 ± 0.035	0.915HisPhe: 0.915 ± 0.031	1.331HisGly: 1.331 ± 0.041	0.48HisHis: 0.48 ± 0.029	1.461HisIle: 1.461 ± 0.04	1.072HisLys: 1.072 ± 0.033	1.644HisLeu: 1.644 ± 0.047	0.609HisMet: 0.609 ± 0.032	0.758HisAsn: 0.758 ± 0.032	0.921HisPro: 0.921 ± 0.034	0.585HisGln: 0.585 ± 0.022	0.821HisArg: 0.821 ± 0.035	0.999HisSer: 0.999 ± 0.035	0.947HisThr: 0.947 ± 0.033	1.191HisVal: 1.191 ± 0.039	0.216HisTrp: 0.216 ± 0.015	0.888HisTyr: 0.888 ± 0.029	0.0HisXaa: 0.0 ± 0.0
Ile	5.591IleAla: 5.591 ± 0.09	1.307IleCys: 1.307 ± 0.039	3.965IleAsp: 3.965 ± 0.071	4.82IleGlu: 4.82 ± 0.081	2.853IlePhe: 2.853 ± 0.068	4.983IleGly: 4.983 ± 0.085	1.517IleHis: 1.517 ± 0.048	4.985IleIle: 4.985 ± 0.081	3.762IleLys: 3.762 ± 0.07	7.354IleLeu: 7.354 ± 0.108	2.038IleMet: 2.038 ± 0.053	2.884IleAsn: 2.884 ± 0.061	3.249IlePro: 3.249 ± 0.06	2.363IleGln: 2.363 ± 0.06	4.094IleArg: 4.094 ± 0.073	4.716IleSer: 4.716 ± 0.08	4.439IleThr: 4.439 ± 0.081	4.921IleVal: 4.921 ± 0.083	0.675IleTrp: 0.675 ± 0.031	3.013IleTyr: 3.013 ± 0.059	0.0IleXaa: 0.0 ± 0.0
Lys	4.721LysAla: 4.721 ± 0.08	0.691LysCys: 0.691 ± 0.026	3.848LysAsp: 3.848 ± 0.074	7.077LysGlu: 7.077 ± 0.104	1.742LysPhe: 1.742 ± 0.046	3.984LysGly: 3.984 ± 0.073	1.046LysHis: 1.046 ± 0.039	4.454LysIle: 4.454 ± 0.076	5.554LysLys: 5.554 ± 0.081	5.22LysLeu: 5.22 ± 0.069	2.066LysMet: 2.066 ± 0.054	3.296LysAsn: 3.296 ± 0.06	1.939LysPro: 1.939 ± 0.048	2.452LysGln: 2.452 ± 0.057	2.9LysArg: 2.9 ± 0.054	3.129LysSer: 3.129 ± 0.063	3.418LysThr: 3.418 ± 0.06	3.997LysVal: 3.997 ± 0.069	0.668LysTrp: 0.668 ± 0.029	2.783LysTyr: 2.783 ± 0.058	0.001LysXaa: 0.001 ± 0.001
Leu	6.476LeuAla: 6.476 ± 0.088	1.666LeuCys: 1.666 ± 0.051	4.646LeuAsp: 4.646 ± 0.077	5.793LeuGlu: 5.793 ± 0.08	4.029LeuPhe: 4.029 ± 0.079	5.705LeuGly: 5.705 ± 0.102	1.713LeuHis: 1.713 ± 0.052	6.198LeuIle: 6.198 ± 0.099	6.094LeuLys: 6.094 ± 0.091	9.429LeuLeu: 9.429 ± 0.128	2.797LeuMet: 2.797 ± 0.051	3.798LeuAsn: 3.798 ± 0.071	3.641LeuPro: 3.641 ± 0.071	3.645LeuGln: 3.645 ± 0.079	3.791LeuArg: 3.791 ± 0.068	6.341LeuSer: 6.341 ± 0.093	5.078LeuThr: 5.078 ± 0.084	5.521LeuVal: 5.521 ± 0.079	0.872LeuTrp: 0.872 ± 0.028	3.757LeuTyr: 3.757 ± 0.081	0.001LeuXaa: 0.001 ± 0.001
Met	2.331MetAla: 2.331 ± 0.049	0.375MetCys: 0.375 ± 0.022	2.0MetAsp: 2.0 ± 0.041	2.817MetGlu: 2.817 ± 0.063	0.949MetPhe: 0.949 ± 0.033	2.071MetGly: 2.071 ± 0.058	0.503MetHis: 0.503 ± 0.025	2.458MetIle: 2.458 ± 0.059	2.745MetLys: 2.745 ± 0.053	2.809MetLeu: 2.809 ± 0.06	0.989MetMet: 0.989 ± 0.038	1.696MetAsn: 1.696 ± 0.04	1.046MetPro: 1.046 ± 0.035	1.289MetGln: 1.289 ± 0.043	1.255MetArg: 1.255 ± 0.042	1.754MetSer: 1.754 ± 0.036	1.767MetThr: 1.767 ± 0.046	2.081MetVal: 2.081 ± 0.05	0.216MetTrp: 0.216 ± 0.015	0.949MetTyr: 0.949 ± 0.032	0.001MetXaa: 0.001 ± 0.001
Asn	3.112AsnAla: 3.112 ± 0.073	0.638AsnCys: 0.638 ± 0.027	2.184AsnAsp: 2.184 ± 0.048	3.01AsnGlu: 3.01 ± 0.066	1.565AsnPhe: 1.565 ± 0.045	3.545AsnGly: 3.545 ± 0.067	0.872AsnHis: 0.872 ± 0.031	3.24AsnIle: 3.24 ± 0.072	2.426AsnLys: 2.426 ± 0.055	3.698AsnLeu: 3.698 ± 0.065	1.401AsnMet: 1.401 ± 0.038	1.842AsnAsn: 1.842 ± 0.048	1.963AsnPro: 1.963 ± 0.054	1.577AsnGln: 1.577 ± 0.046	2.208AsnArg: 2.208 ± 0.056	2.327AsnSer: 2.327 ± 0.06	2.417AsnThr: 2.417 ± 0.058	2.838AsnVal: 2.838 ± 0.059	0.402AsnTrp: 0.402 ± 0.024	1.922AsnTyr: 1.922 ± 0.052	0.0AsnXaa: 0.0 ± 0.0
Pro	2.307ProAla: 2.307 ± 0.055	0.483ProCys: 0.483 ± 0.026	2.483ProAsp: 2.483 ± 0.06	3.568ProGlu: 3.568 ± 0.069	1.518ProPhe: 1.518 ± 0.045	2.29ProGly: 2.29 ± 0.055	0.578ProHis: 0.578 ± 0.024	2.006ProIle: 2.006 ± 0.051	1.754ProLys: 1.754 ± 0.045	2.738ProLeu: 2.738 ± 0.056	0.936ProMet: 0.936 ± 0.033	1.125ProAsn: 1.125 ± 0.041	0.736ProPro: 0.736 ± 0.027	1.051ProGln: 1.051 ± 0.037	0.961ProArg: 0.961 ± 0.033	1.777ProSer: 1.777 ± 0.042	1.433ProThr: 1.433 ± 0.046	3.238ProVal: 3.238 ± 0.06	0.351ProTrp: 0.351 ± 0.02	1.547ProTyr: 1.547 ± 0.047	0.0ProXaa: 0.0 ± 0.0
Gln	2.339GlnAla: 2.339 ± 0.055	0.398GlnCys: 0.398 ± 0.024	1.751GlnAsp: 1.751 ± 0.051	3.091GlnGlu: 3.091 ± 0.079	1.288GlnPhe: 1.288 ± 0.039	2.099GlnGly: 2.099 ± 0.046	0.574GlnHis: 0.574 ± 0.028	2.898GlnIle: 2.898 ± 0.061	2.956GlnLys: 2.956 ± 0.065	2.912GlnLeu: 2.912 ± 0.063	1.36GlnMet: 1.36 ± 0.045	1.841GlnAsn: 1.841 ± 0.055	0.956GlnPro: 0.956 ± 0.035	1.469GlnGln: 1.469 ± 0.053	1.498GlnArg: 1.498 ± 0.047	1.805GlnSer: 1.805 ± 0.051	1.854GlnThr: 1.854 ± 0.051	2.156GlnVal: 2.156 ± 0.049	0.338GlnTrp: 0.338 ± 0.019	1.477GlnTyr: 1.477 ± 0.038	0.0GlnXaa: 0.0 ± 0.0
Arg	2.571ArgAla: 2.571 ± 0.059	0.634ArgCys: 0.634 ± 0.029	2.293ArgAsp: 2.293 ± 0.051	4.048ArgGlu: 4.048 ± 0.084	1.866ArgPhe: 1.866 ± 0.049	2.53ArgGly: 2.53 ± 0.056	0.789ArgHis: 0.789 ± 0.029	3.757ArgIle: 3.757 ± 0.074	3.597ArgLys: 3.597 ± 0.067	3.776ArgLeu: 3.776 ± 0.07	1.689ArgMet: 1.689 ± 0.043	2.235ArgAsn: 2.235 ± 0.056	1.294ArgPro: 1.294 ± 0.042	1.726ArgGln: 1.726 ± 0.047	2.1ArgArg: 2.1 ± 0.061	2.221ArgSer: 2.221 ± 0.059	2.187ArgThr: 2.187 ± 0.052	2.771ArgVal: 2.771 ± 0.06	0.389ArgTrp: 0.389 ± 0.022	1.992ArgTyr: 1.992 ± 0.05	0.0ArgXaa: 0.0 ± 0.0
Ser	3.958SerAla: 3.958 ± 0.076	0.881SerCys: 0.881 ± 0.038	3.179SerAsp: 3.179 ± 0.068	3.834SerGlu: 3.834 ± 0.084	2.831SerPhe: 2.831 ± 0.058	4.654SerGly: 4.654 ± 0.075	1.046SerHis: 1.046 ± 0.034	4.031SerIle: 4.031 ± 0.074	3.012SerLys: 3.012 ± 0.053	5.107SerLeu: 5.107 ± 0.088	2.006SerMet: 2.006 ± 0.05	2.255SerAsn: 2.255 ± 0.044	1.642SerPro: 1.642 ± 0.042	1.791SerGln: 1.791 ± 0.043	2.757SerArg: 2.757 ± 0.054	3.354SerSer: 3.354 ± 0.088	2.848SerThr: 2.848 ± 0.071	4.234SerVal: 4.234 ± 0.074	0.474SerTrp: 0.474 ± 0.025	2.598SerTyr: 2.598 ± 0.059	0.0SerXaa: 0.0 ± 0.0
Thr	4.141ThrAla: 4.141 ± 0.072	0.709ThrCys: 0.709 ± 0.03	3.342ThrAsp: 3.342 ± 0.066	3.937ThrGlu: 3.937 ± 0.072	2.144ThrPhe: 2.144 ± 0.047	4.774ThrGly: 4.774 ± 0.083	0.84ThrHis: 0.84 ± 0.032	4.039ThrIle: 4.039 ± 0.076	2.897ThrLys: 2.897 ± 0.056	4.708ThrLeu: 4.708 ± 0.078	1.529ThrMet: 1.529 ± 0.048	1.958ThrAsn: 1.958 ± 0.052	2.083ThrPro: 2.083 ± 0.044	1.666ThrGln: 1.666 ± 0.048	2.048ThrArg: 2.048 ± 0.054	2.824ThrSer: 2.824 ± 0.06	2.85ThrThr: 2.85 ± 0.069	4.174ThrVal: 4.174 ± 0.077	0.472ThrTrp: 0.472 ± 0.025	2.193ThrTyr: 2.193 ± 0.052	0.0ThrXaa: 0.0 ± 0.0
Val	4.62ValAla: 4.62 ± 0.085	1.258ValCys: 1.258 ± 0.036	3.801ValAsp: 3.801 ± 0.076	4.609ValGlu: 4.609 ± 0.085	3.07ValPhe: 3.07 ± 0.064	3.997ValGly: 3.997 ± 0.083	1.18ValHis: 1.18 ± 0.033	5.317ValIle: 5.317 ± 0.068	4.338ValLys: 4.338 ± 0.083	6.851ValLeu: 6.851 ± 0.096	2.102ValMet: 2.102 ± 0.049	3.008ValAsn: 3.008 ± 0.063	2.496ValPro: 2.496 ± 0.057	2.074ValGln: 2.074 ± 0.049	2.907ValArg: 2.907 ± 0.061	4.547ValSer: 4.547 ± 0.071	4.079ValThr: 4.079 ± 0.069	4.907ValVal: 4.907 ± 0.091	0.665ValTrp: 0.665 ± 0.029	2.835ValTyr: 2.835 ± 0.06	0.0ValXaa: 0.0 ± 0.0
Trp	0.484TrpAla: 0.484 ± 0.028	0.167TrpCys: 0.167 ± 0.014	0.527TrpAsp: 0.527 ± 0.026	0.665TrpGlu: 0.665 ± 0.031	0.439TrpPhe: 0.439 ± 0.023	0.688TrpGly: 0.688 ± 0.033	0.166TrpHis: 0.166 ± 0.014	0.702TrpIle: 0.702 ± 0.032	0.831TrpLys: 0.831 ± 0.032	0.882TrpLeu: 0.882 ± 0.038	0.375TrpMet: 0.375 ± 0.016	0.619TrpAsn: 0.619 ± 0.027	0.249TrpPro: 0.249 ± 0.017	0.394TrpGln: 0.394 ± 0.023	0.338TrpArg: 0.338 ± 0.024	0.474TrpSer: 0.474 ± 0.026	0.443TrpThr: 0.443 ± 0.027	0.515TrpVal: 0.515 ± 0.027	0.134TrpTrp: 0.134 ± 0.013	0.428TrpTyr: 0.428 ± 0.027	0.0TrpXaa: 0.0 ± 0.0
Tyr	2.824TyrAla: 2.824 ± 0.057	0.652TyrCys: 0.652 ± 0.024	2.643TyrAsp: 2.643 ± 0.056	3.47TyrGlu: 3.47 ± 0.066	1.983TyrPhe: 1.983 ± 0.054	3.053TyrGly: 3.053 ± 0.065	1.047TyrHis: 1.047 ± 0.031	2.899TyrIle: 2.899 ± 0.066	2.184TyrLys: 2.184 ± 0.049	4.211TyrLeu: 4.211 ± 0.075	1.202TyrMet: 1.202 ± 0.044	1.788TyrAsn: 1.788 ± 0.048	1.549TyrPro: 1.549 ± 0.046	1.702TyrGln: 1.702 ± 0.052	2.232TyrArg: 2.232 ± 0.047	2.391TyrSer: 2.391 ± 0.062	2.238TyrThr: 2.238 ± 0.051	2.889TyrVal: 2.889 ± 0.055	0.41TyrTrp: 0.41 ± 0.022	2.113TyrTyr: 2.113 ± 0.06	0.0TyrXaa: 0.0 ± 0.0
Xaa	0.0XaaAla: 0.0 ± 0.0	0.0XaaCys: 0.0 ± 0.0	0.0XaaAsp: 0.0 ± 0.0	0.0XaaGlu: 0.0 ± 0.0	0.0XaaPhe: 0.0 ± 0.0	0.001XaaGly: 0.001 ± 0.001	0.0XaaHis: 0.0 ± 0.0	0.0XaaIle: 0.0 ± 0.0	0.0XaaLys: 0.0 ± 0.0	0.0XaaLeu: 0.0 ± 0.0	0.001XaaMet: 0.001 ± 0.001	0.0XaaAsn: 0.0 ± 0.0	0.0XaaPro: 0.0 ± 0.0	0.003XaaGln: 0.003 ± 0.002	0.0XaaArg: 0.0 ± 0.0	0.0XaaSer: 0.0 ± 0.0	0.0XaaThr: 0.0 ± 0.0	0.001XaaVal: 0.001 ± 0.001	0.0XaaTrp: 0.0 ± 0.0	0.001XaaTyr: 0.001 ± 0.001	0.011XaaXaa: 0.011 ± 0.005

Statistics based on 2767 proteins (879927 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

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