Amino acid dipepetide frequency for Brevundimonas sp. Leaf363

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	21.607AlaAla: 21.607 ± 0.237	1.132AlaCys: 1.132 ± 0.036	8.248AlaAsp: 8.248 ± 0.1	8.415AlaGlu: 8.415 ± 0.113	4.966AlaPhe: 4.966 ± 0.088	12.161AlaGly: 12.161 ± 0.153	2.267AlaHis: 2.267 ± 0.063	5.666AlaIle: 5.666 ± 0.078	3.51AlaLys: 3.51 ± 0.08	14.463AlaLeu: 14.463 ± 0.145	3.667AlaMet: 3.667 ± 0.066	2.897AlaAsn: 2.897 ± 0.055	7.607AlaPro: 7.607 ± 0.125	4.661AlaGln: 4.661 ± 0.088	10.491AlaArg: 10.491 ± 0.13	6.622AlaSer: 6.622 ± 0.089	6.408AlaThr: 6.408 ± 0.083	10.461AlaVal: 10.461 ± 0.118	2.141AlaTrp: 2.141 ± 0.055	2.854AlaTyr: 2.854 ± 0.063	0.0AlaXaa: 0.0 ± 0.0
Cys	0.986CysAla: 0.986 ± 0.04	0.063CysCys: 0.063 ± 0.008	0.486CysAsp: 0.486 ± 0.023	0.339CysGlu: 0.339 ± 0.018	0.224CysPhe: 0.224 ± 0.016	0.745CysGly: 0.745 ± 0.03	0.141CysHis: 0.141 ± 0.013	0.29CysIle: 0.29 ± 0.018	0.134CysLys: 0.134 ± 0.012	0.647CysLeu: 0.647 ± 0.028	0.124CysMet: 0.124 ± 0.012	0.169CysAsn: 0.169 ± 0.014	0.394CysPro: 0.394 ± 0.025	0.198CysGln: 0.198 ± 0.016	0.508CysArg: 0.508 ± 0.022	0.34CysSer: 0.34 ± 0.019	0.321CysThr: 0.321 ± 0.021	0.57CysVal: 0.57 ± 0.024	0.11CysTrp: 0.11 ± 0.011	0.133CysTyr: 0.133 ± 0.013	0.0CysXaa: 0.0 ± 0.0
Asp	8.801AspAla: 8.801 ± 0.119	0.393AspCys: 0.393 ± 0.02	3.767AspAsp: 3.767 ± 0.07	3.264AspGlu: 3.264 ± 0.069	2.19AspPhe: 2.19 ± 0.048	6.112AspGly: 6.112 ± 0.094	1.364AspHis: 1.364 ± 0.038	2.651AspIle: 2.651 ± 0.066	1.528AspLys: 1.528 ± 0.047	6.286AspLeu: 6.286 ± 0.095	1.247AspMet: 1.247 ± 0.039	1.14AspAsn: 1.14 ± 0.034	3.953AspPro: 3.953 ± 0.066	2.215AspGln: 2.215 ± 0.054	5.05AspArg: 5.05 ± 0.07	2.08AspSer: 2.08 ± 0.055	2.538AspThr: 2.538 ± 0.06	4.466AspVal: 4.466 ± 0.072	1.246AspTrp: 1.246 ± 0.039	1.556AspTyr: 1.556 ± 0.042	0.0AspXaa: 0.0 ± 0.0
Glu	9.072GluAla: 9.072 ± 0.11	0.235GluCys: 0.235 ± 0.018	2.879GluAsp: 2.879 ± 0.06	2.504GluGlu: 2.504 ± 0.057	1.409GluPhe: 1.409 ± 0.038	4.888GluGly: 4.888 ± 0.083	1.089GluHis: 1.089 ± 0.034	2.752GluIle: 2.752 ± 0.054	1.589GluLys: 1.589 ± 0.051	4.616GluLeu: 4.616 ± 0.085	1.333GluMet: 1.333 ± 0.039	1.143GluAsn: 1.143 ± 0.039	3.016GluPro: 3.016 ± 0.073	1.936GluGln: 1.936 ± 0.047	5.026GluArg: 5.026 ± 0.085	2.021GluSer: 2.021 ± 0.046	3.83GluThr: 3.83 ± 0.071	3.773GluVal: 3.773 ± 0.062	0.689GluTrp: 0.689 ± 0.027	0.878GluTyr: 0.878 ± 0.03	0.0GluXaa: 0.0 ± 0.0
Phe	4.645PheAla: 4.645 ± 0.087	0.313PheCys: 0.313 ± 0.018	2.834PheAsp: 2.834 ± 0.06	2.23PheGlu: 2.23 ± 0.045	1.217PhePhe: 1.217 ± 0.043	3.516PheGly: 3.516 ± 0.064	0.68PheHis: 0.68 ± 0.031	1.455PheIle: 1.455 ± 0.037	0.872PheLys: 0.872 ± 0.035	2.803PheLeu: 2.803 ± 0.061	0.753PheMet: 0.753 ± 0.027	1.016PheAsn: 1.016 ± 0.033	1.343PhePro: 1.343 ± 0.043	1.034PheGln: 1.034 ± 0.034	2.104PheArg: 2.104 ± 0.047	1.878PheSer: 1.878 ± 0.041	1.984PheThr: 1.984 ± 0.049	2.643PheVal: 2.643 ± 0.053	0.527PheTrp: 0.527 ± 0.023	0.86PheTyr: 0.86 ± 0.035	0.0PheXaa: 0.0 ± 0.0
Gly	11.522GlyAla: 11.522 ± 0.128	0.761GlyCys: 0.761 ± 0.03	5.474GlyAsp: 5.474 ± 0.094	5.021GlyGlu: 5.021 ± 0.085	3.626GlyPhe: 3.626 ± 0.061	8.733GlyGly: 8.733 ± 0.139	1.66GlyHis: 1.66 ± 0.047	2.834GlyIle: 2.834 ± 0.064	2.572GlyLys: 2.572 ± 0.065	9.577GlyLeu: 9.577 ± 0.113	2.161GlyMet: 2.161 ± 0.05	1.508GlyAsn: 1.508 ± 0.048	4.269GlyPro: 4.269 ± 0.069	3.438GlyGln: 3.438 ± 0.053	7.385GlyArg: 7.385 ± 0.104	4.538GlySer: 4.538 ± 0.085	3.375GlyThr: 3.375 ± 0.075	8.22GlyVal: 8.22 ± 0.089	1.81GlyTrp: 1.81 ± 0.049	2.335GlyTyr: 2.335 ± 0.06	0.0GlyXaa: 0.0 ± 0.0
His	2.41HisAla: 2.41 ± 0.058	0.157HisCys: 0.157 ± 0.014	1.192HisAsp: 1.192 ± 0.041	0.978HisGlu: 0.978 ± 0.034	0.65HisPhe: 0.65 ± 0.026	1.82HisGly: 1.82 ± 0.05	0.48HisHis: 0.48 ± 0.026	0.742HisIle: 0.742 ± 0.03	0.357HisLys: 0.357 ± 0.017	1.713HisLeu: 1.713 ± 0.048	0.431HisMet: 0.431 ± 0.02	0.388HisAsn: 0.388 ± 0.02	1.212HisPro: 1.212 ± 0.038	0.561HisGln: 0.561 ± 0.024	1.355HisArg: 1.355 ± 0.043	0.769HisSer: 0.769 ± 0.028	0.74HisThr: 0.74 ± 0.029	1.372HisVal: 1.372 ± 0.041	0.325HisTrp: 0.325 ± 0.02	0.427HisTyr: 0.427 ± 0.021	0.0HisXaa: 0.0 ± 0.0
Ile	6.193IleAla: 6.193 ± 0.086	0.369IleCys: 0.369 ± 0.02	3.568IleAsp: 3.568 ± 0.067	3.128IleGlu: 3.128 ± 0.065	1.271IlePhe: 1.271 ± 0.04	4.565IleGly: 4.565 ± 0.087	0.805IleHis: 0.805 ± 0.029	1.647IleIle: 1.647 ± 0.05	1.172IleLys: 1.172 ± 0.041	3.743IleLeu: 3.743 ± 0.07	0.729IleMet: 0.729 ± 0.032	1.107IleAsn: 1.107 ± 0.038	1.889IlePro: 1.889 ± 0.047	1.324IleGln: 1.324 ± 0.038	2.922IleArg: 2.922 ± 0.061	2.099IleSer: 2.099 ± 0.041	2.327IleThr: 2.327 ± 0.052	3.617IleVal: 3.617 ± 0.069	0.569IleTrp: 0.569 ± 0.025	0.877IleTyr: 0.877 ± 0.032	0.0IleXaa: 0.0 ± 0.0
Lys	4.355LysAla: 4.355 ± 0.085	0.104LysCys: 0.104 ± 0.011	1.432LysAsp: 1.432 ± 0.049	1.011LysGlu: 1.011 ± 0.042	0.695LysPhe: 0.695 ± 0.029	2.399LysGly: 2.399 ± 0.05	0.46LysHis: 0.46 ± 0.022	1.211LysIle: 1.211 ± 0.04	0.96LysLys: 0.96 ± 0.043	2.467LysLeu: 2.467 ± 0.055	0.541LysMet: 0.541 ± 0.022	0.595LysAsn: 0.595 ± 0.027	1.879LysPro: 1.879 ± 0.05	0.668LysGln: 0.668 ± 0.029	2.044LysArg: 2.044 ± 0.051	1.415LysSer: 1.415 ± 0.041	1.791LysThr: 1.791 ± 0.044	2.05LysVal: 2.05 ± 0.053	0.288LysTrp: 0.288 ± 0.017	0.489LysTyr: 0.489 ± 0.026	0.0LysXaa: 0.0 ± 0.0
Leu	13.714LeuAla: 13.714 ± 0.163	0.663LeuCys: 0.663 ± 0.025	6.205LeuAsp: 6.205 ± 0.085	5.101LeuGlu: 5.101 ± 0.084	3.452LeuPhe: 3.452 ± 0.071	8.213LeuGly: 8.213 ± 0.115	1.638LeuHis: 1.638 ± 0.047	5.081LeuIle: 5.081 ± 0.082	3.648LeuLys: 3.648 ± 0.062	8.298LeuLeu: 8.298 ± 0.114	2.364LeuMet: 2.364 ± 0.052	2.777LeuAsn: 2.777 ± 0.058	5.038LeuPro: 5.038 ± 0.075	2.647LeuGln: 2.647 ± 0.057	6.418LeuArg: 6.418 ± 0.092	6.183LeuSer: 6.183 ± 0.089	6.213LeuThr: 6.213 ± 0.085	6.621LeuVal: 6.621 ± 0.096	1.237LeuTrp: 1.237 ± 0.048	1.94LeuTyr: 1.94 ± 0.047	0.0LeuXaa: 0.0 ± 0.0
Met	3.406MetAla: 3.406 ± 0.064	0.101MetCys: 0.101 ± 0.011	1.24MetAsp: 1.24 ± 0.041	1.01MetGlu: 1.01 ± 0.038	0.65MetPhe: 0.65 ± 0.028	1.965MetGly: 1.965 ± 0.05	0.379MetHis: 0.379 ± 0.019	1.167MetIle: 1.167 ± 0.035	0.825MetLys: 0.825 ± 0.029	2.05MetLeu: 2.05 ± 0.043	0.597MetMet: 0.597 ± 0.028	0.667MetAsn: 0.667 ± 0.026	1.21MetPro: 1.21 ± 0.037	0.734MetGln: 0.734 ± 0.031	1.785MetArg: 1.785 ± 0.044	1.544MetSer: 1.544 ± 0.039	1.961MetThr: 1.961 ± 0.038	1.477MetVal: 1.477 ± 0.04	0.215MetTrp: 0.215 ± 0.016	0.236MetTyr: 0.236 ± 0.017	0.0MetXaa: 0.0 ± 0.0
Asn	3.126AsnAla: 3.126 ± 0.061	0.171AsnCys: 0.171 ± 0.015	1.309AsnAsp: 1.309 ± 0.041	1.001AsnGlu: 1.001 ± 0.035	0.727AsnPhe: 0.727 ± 0.032	2.256AsnGly: 2.256 ± 0.061	0.432AsnHis: 0.432 ± 0.021	1.021AsnIle: 1.021 ± 0.035	0.451AsnLys: 0.451 ± 0.023	2.297AsnLeu: 2.297 ± 0.054	0.462AsnMet: 0.462 ± 0.023	0.512AsnAsn: 0.512 ± 0.028	1.671AsnPro: 1.671 ± 0.041	0.71AsnGln: 0.71 ± 0.026	1.727AsnArg: 1.727 ± 0.048	0.995AsnSer: 0.995 ± 0.041	1.124AsnThr: 1.124 ± 0.037	1.719AsnVal: 1.719 ± 0.051	0.337AsnTrp: 0.337 ± 0.02	0.565AsnTyr: 0.565 ± 0.031	0.0AsnXaa: 0.0 ± 0.0
Pro	7.64ProAla: 7.64 ± 0.124	0.298ProCys: 0.298 ± 0.015	3.978ProAsp: 3.978 ± 0.067	3.701ProGlu: 3.701 ± 0.067	1.983ProPhe: 1.983 ± 0.055	5.08ProGly: 5.08 ± 0.078	0.961ProHis: 0.961 ± 0.032	2.295ProIle: 2.295 ± 0.055	1.368ProLys: 1.368 ± 0.045	4.753ProLeu: 4.753 ± 0.077	1.207ProMet: 1.207 ± 0.034	1.299ProAsn: 1.299 ± 0.042	2.956ProPro: 2.956 ± 0.09	1.719ProGln: 1.719 ± 0.049	3.272ProArg: 3.272 ± 0.065	2.88ProSer: 2.88 ± 0.058	2.919ProThr: 2.919 ± 0.06	4.376ProVal: 4.376 ± 0.067	0.868ProTrp: 0.868 ± 0.031	1.14ProTyr: 1.14 ± 0.038	0.0ProXaa: 0.0 ± 0.0
Gln	5.358GlnAla: 5.358 ± 0.086	0.161GlnCys: 0.161 ± 0.013	1.569GlnAsp: 1.569 ± 0.046	1.246GlnGlu: 1.246 ± 0.038	0.939GlnPhe: 0.939 ± 0.032	2.779GlnGly: 2.779 ± 0.069	0.554GlnHis: 0.554 ± 0.023	1.554GlnIle: 1.554 ± 0.041	0.785GlnLys: 0.785 ± 0.031	2.631GlnLeu: 2.631 ± 0.061	0.829GlnMet: 0.829 ± 0.027	0.72GlnAsn: 0.72 ± 0.032	2.073GlnPro: 2.073 ± 0.045	1.042GlnGln: 1.042 ± 0.038	2.551GlnArg: 2.551 ± 0.052	1.806GlnSer: 1.806 ± 0.043	2.155GlnThr: 2.155 ± 0.05	2.432GlnVal: 2.432 ± 0.055	0.398GlnTrp: 0.398 ± 0.021	0.558GlnTyr: 0.558 ± 0.028	0.0GlnXaa: 0.0 ± 0.0
Arg	9.429ArgAla: 9.429 ± 0.113	0.438ArgCys: 0.438 ± 0.023	4.447ArgAsp: 4.447 ± 0.076	3.829ArgGlu: 3.829 ± 0.067	3.048ArgPhe: 3.048 ± 0.057	5.375ArgGly: 5.375 ± 0.101	1.372ArgHis: 1.372 ± 0.039	3.882ArgIle: 3.882 ± 0.06	1.86ArgLys: 1.86 ± 0.047	8.948ArgLeu: 8.948 ± 0.112	1.964ArgMet: 1.964 ± 0.041	1.498ArgAsn: 1.498 ± 0.041	4.372ArgPro: 4.372 ± 0.072	2.506ArgGln: 2.506 ± 0.057	6.432ArgArg: 6.432 ± 0.109	3.552ArgSer: 3.552 ± 0.064	3.781ArgThr: 3.781 ± 0.062	5.265ArgVal: 5.265 ± 0.087	1.241ArgTrp: 1.241 ± 0.041	1.759ArgTyr: 1.759 ± 0.045	0.0ArgXaa: 0.0 ± 0.0
Ser	6.306SerAla: 6.306 ± 0.087	0.29SerCys: 0.29 ± 0.019	3.192SerAsp: 3.192 ± 0.06	2.602SerGlu: 2.602 ± 0.059	1.751SerPhe: 1.751 ± 0.047	5.176SerGly: 5.176 ± 0.085	0.915SerHis: 0.915 ± 0.031	2.25SerIle: 2.25 ± 0.046	1.197SerLys: 1.197 ± 0.042	5.15SerLeu: 5.15 ± 0.075	1.152SerMet: 1.152 ± 0.035	1.238SerAsn: 1.238 ± 0.035	2.879SerPro: 2.879 ± 0.058	1.663SerGln: 1.663 ± 0.046	3.523SerArg: 3.523 ± 0.06	2.556SerSer: 2.556 ± 0.065	2.572SerThr: 2.572 ± 0.059	3.785SerVal: 3.785 ± 0.057	0.761SerTrp: 0.761 ± 0.028	1.18SerTyr: 1.18 ± 0.038	0.0SerXaa: 0.0 ± 0.0
Thr	7.22ThrAla: 7.22 ± 0.091	0.373ThrCys: 0.373 ± 0.022	3.167ThrAsp: 3.167 ± 0.065	2.525ThrGlu: 2.525 ± 0.051	1.842ThrPhe: 1.842 ± 0.05	5.45ThrGly: 5.45 ± 0.091	0.93ThrHis: 0.93 ± 0.038	2.19ThrIle: 2.19 ± 0.051	1.126ThrLys: 1.126 ± 0.036	5.737ThrLeu: 5.737 ± 0.073	0.929ThrMet: 0.929 ± 0.031	1.182ThrAsn: 1.182 ± 0.037	4.228ThrPro: 4.228 ± 0.07	1.536ThrGln: 1.536 ± 0.042	3.465ThrArg: 3.465 ± 0.064	2.42ThrSer: 2.42 ± 0.05	2.905ThrThr: 2.905 ± 0.071	4.388ThrVal: 4.388 ± 0.089	0.738ThrTrp: 0.738 ± 0.033	1.18ThrTyr: 1.18 ± 0.04	0.0ThrXaa: 0.0 ± 0.0
Val	10.081ValAla: 10.081 ± 0.111	0.604ValCys: 0.604 ± 0.03	4.496ValAsp: 4.496 ± 0.072	5.003ValGlu: 5.003 ± 0.084	2.716ValPhe: 2.716 ± 0.063	6.207ValGly: 6.207 ± 0.092	1.278ValHis: 1.278 ± 0.039	3.772ValIle: 3.772 ± 0.064	1.945ValLys: 1.945 ± 0.054	7.467ValLeu: 7.467 ± 0.101	1.907ValMet: 1.907 ± 0.045	1.792ValAsn: 1.792 ± 0.052	2.882ValPro: 2.882 ± 0.059	2.397ValGln: 2.397 ± 0.059	5.657ValArg: 5.657 ± 0.074	4.296ValSer: 4.296 ± 0.075	4.683ValThr: 4.683 ± 0.077	6.693ValVal: 6.693 ± 0.097	1.188ValTrp: 1.188 ± 0.037	1.525ValTyr: 1.525 ± 0.044	0.0ValXaa: 0.0 ± 0.0
Trp	1.663TrpAla: 1.663 ± 0.042	0.116TrpCys: 0.116 ± 0.01	0.78TrpAsp: 0.78 ± 0.031	0.543TrpGlu: 0.543 ± 0.026	0.594TrpPhe: 0.594 ± 0.024	1.154TrpGly: 1.154 ± 0.036	0.26TrpHis: 0.26 ± 0.017	0.723TrpIle: 0.723 ± 0.028	0.487TrpLys: 0.487 ± 0.022	1.817TrpLeu: 1.817 ± 0.053	0.413TrpMet: 0.413 ± 0.022	0.445TrpAsn: 0.445 ± 0.02	0.83TrpPro: 0.83 ± 0.033	0.42TrpGln: 0.42 ± 0.024	1.487TrpArg: 1.487 ± 0.041	1.064TrpSer: 1.064 ± 0.034	1.01TrpThr: 1.01 ± 0.036	0.956TrpVal: 0.956 ± 0.032	0.282TrpTrp: 0.282 ± 0.017	0.258TrpTyr: 0.258 ± 0.018	0.0TrpXaa: 0.0 ± 0.0
Tyr	2.71TyrAla: 2.71 ± 0.055	0.178TyrCys: 0.178 ± 0.012	1.585TyrAsp: 1.585 ± 0.051	1.226TyrGlu: 1.226 ± 0.035	0.758TyrPhe: 0.758 ± 0.029	2.226TyrGly: 2.226 ± 0.062	0.414TyrHis: 0.414 ± 0.021	0.738TyrIle: 0.738 ± 0.026	0.467TyrLys: 0.467 ± 0.025	1.988TyrLeu: 1.988 ± 0.047	0.407TyrMet: 0.407 ± 0.023	0.518TyrAsn: 0.518 ± 0.029	0.996TyrPro: 0.996 ± 0.031	0.73TyrGln: 0.73 ± 0.029	1.799TyrArg: 1.799 ± 0.045	1.079TyrSer: 1.079 ± 0.042	0.894TyrThr: 0.894 ± 0.038	1.707TyrVal: 1.707 ± 0.042	0.329TyrTrp: 0.329 ± 0.022	0.503TyrTyr: 0.503 ± 0.025	0.0TyrXaa: 0.0 ± 0.0
Xaa	0.0XaaAla: 0.0 ± 0.0	0.0XaaCys: 0.0 ± 0.0	0.0XaaAsp: 0.0 ± 0.0	0.0XaaGlu: 0.0 ± 0.0	0.0XaaPhe: 0.0 ± 0.0	0.0XaaGly: 0.0 ± 0.0	0.0XaaHis: 0.0 ± 0.0	0.0XaaIle: 0.0 ± 0.0	0.0XaaLys: 0.0 ± 0.0	0.0XaaLeu: 0.0 ± 0.0	0.0XaaMet: 0.0 ± 0.0	0.0XaaAsn: 0.0 ± 0.0	0.0XaaPro: 0.0 ± 0.0	0.0XaaGln: 0.0 ± 0.0	0.0XaaArg: 0.0 ± 0.0	0.0XaaSer: 0.0 ± 0.0	0.0XaaThr: 0.0 ± 0.0	0.0XaaVal: 0.0 ± 0.0	0.0XaaTrp: 0.0 ± 0.0	0.0XaaTyr: 0.0 ± 0.0	0.0XaaXaa: 0.0 ± 0.0

Statistics based on 2955 proteins (935029 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

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