Burkholderia sp. SRS-W-2-2016

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Betaproteobacteria; Burkholderiales; Burkholderiaceae; Burkholderia; unclassified Burkholderia

Average proteome isoelectric point is 6.7

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 6582 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1Q8J448|A0A1Q8J448_9BURK Thiol reductase thioredoxin OS=Burkholderia sp. SRS-W-2-2016 OX=1926878 GN=BTH42_00450 PE=4 SV=1
MM1 pKa = 7.87PYY3 pKa = 10.22AYY5 pKa = 9.93DD6 pKa = 3.11GRR8 pKa = 11.84TFYY11 pKa = 10.99FSDD14 pKa = 3.5LDD16 pKa = 4.24GYY18 pKa = 9.44NKK20 pKa = 10.15RR21 pKa = 11.84YY22 pKa = 9.46AARR25 pKa = 11.84LPVEE29 pKa = 4.22EE30 pKa = 5.1YY31 pKa = 10.44EE32 pKa = 4.17IQLVDD37 pKa = 3.9GSDD40 pKa = 4.14AEE42 pKa = 4.21AQLFNLLGVNQATLEE57 pKa = 3.97RR58 pKa = 11.84WFDD61 pKa = 3.62EE62 pKa = 4.63VVDD65 pKa = 4.15LTEE68 pKa = 4.65DD69 pKa = 3.2QHH71 pKa = 7.35AALAFLVGDD80 pKa = 4.85LGLSLSDD87 pKa = 5.24ALDD90 pKa = 3.58KK91 pKa = 10.87MDD93 pKa = 5.24DD94 pKa = 3.47VNLRR98 pKa = 11.84EE99 pKa = 4.18GSLLEE104 pKa = 4.16AATEE108 pKa = 4.41FFDD111 pKa = 4.17EE112 pKa = 5.59CYY114 pKa = 11.04LNDD117 pKa = 3.25IPEE120 pKa = 4.22AVRR123 pKa = 11.84FYY125 pKa = 10.9IDD127 pKa = 2.98YY128 pKa = 10.78DD129 pKa = 3.51AFARR133 pKa = 11.84DD134 pKa = 3.77QNLSGAMSEE143 pKa = 5.19LEE145 pKa = 4.32FAGQTYY151 pKa = 8.11TVTNADD157 pKa = 3.1ARR159 pKa = 4.03

Molecular weight: 17.98 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1Q8IV80|A0A1Q8IV80_9BURK CTP synthase OS=Burkholderia sp. SRS-W-2-2016 OX=1926878 GN=pyrG PE=3 SV=1
MM1 pKa = 7.35KK2 pKa = 10.46GEE4 pKa = 4.09SRR6 pKa = 11.84AKK8 pKa = 9.13RR9 pKa = 11.84TKK11 pKa = 10.21CGSRR15 pKa = 11.84VQPLNSAEE23 pKa = 4.18PPSLACRR30 pKa = 11.84MRR32 pKa = 11.84RR33 pKa = 11.84ASRR36 pKa = 11.84RR37 pKa = 11.84KK38 pKa = 7.88RR39 pKa = 11.84ANRR42 pKa = 11.84ARR44 pKa = 11.84STNHH48 pKa = 4.39AHH50 pKa = 6.55RR51 pKa = 11.84PKK53 pKa = 10.29LARR56 pKa = 11.84RR57 pKa = 11.84MHH59 pKa = 6.54RR60 pKa = 11.84ATRR63 pKa = 11.84RR64 pKa = 11.84KK65 pKa = 9.27RR66 pKa = 11.84ANRR69 pKa = 11.84ALNTNHH75 pKa = 6.64AQLPKK80 pKa = 9.91LASRR84 pKa = 11.84MRR86 pKa = 11.84RR87 pKa = 11.84AARR90 pKa = 11.84RR91 pKa = 11.84KK92 pKa = 8.01RR93 pKa = 11.84ANRR96 pKa = 11.84ARR98 pKa = 11.84RR99 pKa = 11.84TNDD102 pKa = 2.61AQRR105 pKa = 11.84PKK107 pKa = 10.49LVSKK111 pKa = 7.11THH113 pKa = 5.3HH114 pKa = 5.22VRR116 pKa = 11.84RR117 pKa = 11.84GKK119 pKa = 9.75HH120 pKa = 5.42ANRR123 pKa = 11.84ALNTNHH129 pKa = 5.73TQQPKK134 pKa = 9.13LASRR138 pKa = 11.84MHH140 pKa = 6.28RR141 pKa = 11.84VTRR144 pKa = 11.84SKK146 pKa = 10.63RR147 pKa = 11.84ANHH150 pKa = 6.88ALRR153 pKa = 11.84TNDD156 pKa = 3.26AQRR159 pKa = 11.84LKK161 pKa = 10.52HH162 pKa = 6.08VSRR165 pKa = 11.84THH167 pKa = 5.5HH168 pKa = 5.89ARR170 pKa = 11.84RR171 pKa = 11.84GKK173 pKa = 9.65HH174 pKa = 5.84ANRR177 pKa = 11.84ALNTNRR183 pKa = 11.84AQQPKK188 pKa = 9.43LASRR192 pKa = 11.84MHH194 pKa = 6.63RR195 pKa = 11.84ATRR198 pKa = 11.84SKK200 pKa = 10.43RR201 pKa = 11.84ANPANRR207 pKa = 11.84TRR209 pKa = 11.84HH210 pKa = 5.76ASPASHH216 pKa = 7.52ASRR219 pKa = 11.84AA220 pKa = 3.65

Molecular weight: 25.43 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

6582

0

6582

2176520

27

4770

330.7

35.81

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

12.98 ± 0.049

0.904 ± 0.011

5.397 ± 0.023

5.011 ± 0.029

3.694 ± 0.022

8.278 ± 0.037

2.259 ± 0.015

4.61 ± 0.023

2.98 ± 0.026

10.318 ± 0.035

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.274 ± 0.015

2.948 ± 0.029

5.06 ± 0.026

3.729 ± 0.022

6.839 ± 0.038

5.768 ± 0.036

5.498 ± 0.046

7.629 ± 0.023

1.365 ± 0.011

2.459 ± 0.017
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski