Murine roseolovirus
Average proteome isoelectric point is 6.87
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 128 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A1P8VIR2|A0A1P8VIR2_9BETA Uncharacterized protein OS=Murine roseolovirus OX=1940555 GN=ORF10 PE=4 SV=1
MM1 pKa = 7.98 CYY3 pKa = 10.57 HH4 pKa = 7.15 MLPSHH9 pKa = 5.53 QQEE12 pKa = 3.69 EE13 pKa = 4.7 MEE15 pKa = 4.54 NNEE18 pKa = 4.15 SSDD21 pKa = 3.77 TVEE24 pKa = 4.38 ATPSDD29 pKa = 3.84 TDD31 pKa = 3.9 QVSLPHH37 pKa = 6.71 GNPDD41 pKa = 3.35 SPTDD45 pKa = 3.5 GTAYY49 pKa = 9.8 PDD51 pKa = 3.84 LHH53 pKa = 7.12 DD54 pKa = 5.58 LPPPIIDD61 pKa = 5.4 PITGTVVLTLLEE73 pKa = 4.51 MPAPPHH79 pKa = 6.87 PPTPPPIPPRR89 pKa = 11.84 TYY91 pKa = 9.93 KK92 pKa = 10.62 PMLITHH98 pKa = 6.54 PEE100 pKa = 3.86 EE101 pKa = 6.56 DD102 pKa = 3.93 EE103 pKa = 4.27 EE104 pKa = 4.65 QLDD107 pKa = 3.78 RR108 pKa = 6.35
Molecular weight: 11.96 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.847
IPC2_protein 4.101
IPC_protein 4.037
Toseland 3.859
ProMoST 4.19
Dawson 4.012
Bjellqvist 4.164
Wikipedia 3.923
Rodwell 3.872
Grimsley 3.77
Solomon 3.999
Lehninger 3.961
Nozaki 4.139
DTASelect 4.317
Thurlkill 3.897
EMBOSS 3.935
Sillero 4.164
Patrickios 1.939
IPC_peptide 4.012
IPC2_peptide 4.139
IPC2.peptide.svr19 4.039
Protein with the highest isoelectric point:
>tr|A0A1P8VIZ9|A0A1P8VIZ9_9BETA Uncharacterized protein OS=Murine roseolovirus OX=1940555 GN=ORF111 PE=4 SV=1
MM1 pKa = 7.82 IDD3 pKa = 3.29 TEE5 pKa = 4.66 QFLEE9 pKa = 4.47 CDD11 pKa = 3.46 HH12 pKa = 6.23 GHH14 pKa = 6.4 CHH16 pKa = 6.68 TIAISGNSADD26 pKa = 4.56 LRR28 pKa = 11.84 PLVAVRR34 pKa = 11.84 FSFFVFRR41 pKa = 11.84 ALFCHH46 pKa = 6.61 HH47 pKa = 7.33 GDD49 pKa = 3.91 GPLPSPPPSPWQPLLPLRR67 pKa = 11.84 AARR70 pKa = 11.84 VTTVVLIRR78 pKa = 11.84 TPPVRR83 pKa = 11.84 LGCPLTGVAMIKK95 pKa = 8.37 TAPVSAAAPLPCVSVSVSACRR116 pKa = 11.84 CRR118 pKa = 11.84 VSCVPSLGVSHH129 pKa = 7.21 FSSSYY134 pKa = 10.42 SSPLGPSPTPHH145 pKa = 7.31 PSLTAAATSARR156 pKa = 11.84 QTLAPSSHH164 pKa = 5.41 GRR166 pKa = 11.84 EE167 pKa = 3.98 EE168 pKa = 3.83 QTLDD172 pKa = 3.36 FLQSRR177 pKa = 11.84 DD178 pKa = 3.31 RR179 pKa = 11.84 DD180 pKa = 3.8 FLTKK184 pKa = 10.3 KK185 pKa = 10.08 RR186 pKa = 11.84 DD187 pKa = 3.56 RR188 pKa = 11.84 FQIWYY193 pKa = 9.4 LPLNGSCCRR202 pKa = 11.84 RR203 pKa = 11.84 CCWTLCLTAKK213 pKa = 10.29 PSVFSPSRR221 pKa = 11.84 GCLWSAAVAAPPRR234 pKa = 11.84 SSPTPPGALAAAPPAEE250 pKa = 4.74 PGNRR254 pKa = 11.84 SCGGPPPTNSGRR266 pKa = 11.84 TSGPRR271 pKa = 11.84 MIGCTTSRR279 pKa = 11.84 LRR281 pKa = 11.84 TGSAPCAPPCYY292 pKa = 9.88 GVGASWPPPGPPGGACTWNRR312 pKa = 11.84 TPTASAWPSTRR323 pKa = 11.84 CPSPVSVSAGRR334 pKa = 11.84 PSPGPPTRR342 pKa = 11.84 WNLSVRR348 pKa = 11.84 MLFPCLPLPKK358 pKa = 9.95 FPFPPHH364 pKa = 6.94 PSPTPLTSFVSACLSSAVSHH384 pKa = 5.98 VVLSSGVVPLFGVSRR399 pKa = 11.84 DD400 pKa = 3.66 YY401 pKa = 11.37 VPPRR405 pKa = 11.84 RR406 pKa = 11.84 VGGEE410 pKa = 3.53 RR411 pKa = 11.84 LVWFFCRR418 pKa = 11.84 KK419 pKa = 8.39 EE420 pKa = 3.96 KK421 pKa = 9.55 KK422 pKa = 8.01 TCFSILSRR430 pKa = 11.84 RR431 pKa = 11.84 PLLSLLAPSRR441 pKa = 11.84 SPLSVLCSPLAPSRR455 pKa = 11.84 LPVRR459 pKa = 11.84 IFPLSSPSLRR469 pKa = 11.84 VPHH472 pKa = 6.34 PHH474 pKa = 7.16 PLSCLSLPRR483 pKa = 11.84 SEE485 pKa = 4.46 RR486 pKa = 11.84 APIQVLWGHH495 pKa = 6.17 RR496 pKa = 11.84 VFLPGGLHH504 pKa = 6.18 RR505 pKa = 11.84 ASPRR509 pKa = 11.84 AA510 pKa = 3.35
Molecular weight: 54.37 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.339
IPC2_protein 9.355
IPC_protein 10.189
Toseland 10.482
ProMoST 10.292
Dawson 10.599
Bjellqvist 10.423
Wikipedia 10.833
Rodwell 10.613
Grimsley 10.628
Solomon 10.745
Lehninger 10.716
Nozaki 10.657
DTASelect 10.365
Thurlkill 10.511
EMBOSS 10.906
Sillero 10.584
Patrickios 10.292
IPC_peptide 10.745
IPC2_peptide 10.145
IPC2.peptide.svr19 8.592
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
128
0
128
50423
105
2011
393.9
45.15
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
4.012 ± 0.237
2.303 ± 0.127
5.503 ± 0.186
5.648 ± 0.196
5.317 ± 0.195
4.155 ± 0.295
2.366 ± 0.097
8.611 ± 0.343
7.126 ± 0.275
9.101 ± 0.227
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.39 ± 0.092
6.715 ± 0.31
5.091 ± 0.408
3.407 ± 0.153
4.107 ± 0.258
8.012 ± 0.422
5.948 ± 0.177
5.053 ± 0.149
0.837 ± 0.056
4.298 ± 0.174
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here