Odonata-associated circular virus-18
Average proteome isoelectric point is 8.13
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 2 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A0B4UI18|A0A0B4UI18_9VIRU Putative capsid protein OS=Odonata-associated circular virus-18 OX=1592118 PE=4 SV=1
MM1 pKa = 7.47 SKK3 pKa = 7.24 TTRR6 pKa = 11.84 FCFTKK11 pKa = 10.41 NNYY14 pKa = 9.58 SEE16 pKa = 4.41 LHH18 pKa = 5.35 VDD20 pKa = 3.23 ILKK23 pKa = 10.57 SDD25 pKa = 3.6 LTSTFKK31 pKa = 10.65 YY32 pKa = 7.72 WCFGIEE38 pKa = 4.14 VGEE41 pKa = 4.34 SGTPHH46 pKa = 5.66 LQGYY50 pKa = 8.41 FEE52 pKa = 5.0 FPNKK56 pKa = 10.17 NPLRR60 pKa = 11.84 ISGAQNKK67 pKa = 9.37 LNNIGLEE74 pKa = 4.14 GCAIFPAKK82 pKa = 8.95 GTAEE86 pKa = 3.94 QNITYY91 pKa = 9.78 CSKK94 pKa = 10.85 DD95 pKa = 3.1 GSFFEE100 pKa = 4.87 GGEE103 pKa = 4.04 RR104 pKa = 11.84 PRR106 pKa = 11.84 GQGKK110 pKa = 7.81 RR111 pKa = 11.84 TDD113 pKa = 3.84 LDD115 pKa = 4.11 SVCEE119 pKa = 4.33 LIKK122 pKa = 10.95 SGANMTEE129 pKa = 4.21 VANQFPSQVVKK140 pKa = 9.84 FHH142 pKa = 6.99 SGLQFLMNVTITPRR156 pKa = 11.84 RR157 pKa = 11.84 FKK159 pKa = 11.03 TEE161 pKa = 3.88 VWWLWGPTGSGKK173 pKa = 10.09 SRR175 pKa = 11.84 YY176 pKa = 9.56 AWDD179 pKa = 4.1 QYY181 pKa = 10.06 PSSYY185 pKa = 10.11 MKK187 pKa = 10.42 EE188 pKa = 4.6 CSHH191 pKa = 6.34 KK192 pKa = 9.73 WWDD195 pKa = 3.71 GYY197 pKa = 9.37 TGQDD201 pKa = 3.48 VVIMDD206 pKa = 4.59 DD207 pKa = 3.82 FRR209 pKa = 11.84 PSKK212 pKa = 9.96 EE213 pKa = 4.09 LPFSFILNLFDD224 pKa = 5.56 RR225 pKa = 11.84 YY226 pKa = 9.75 PLSVQVKK233 pKa = 8.62 GGMAQFVSKK242 pKa = 9.31 TIYY245 pKa = 9.1 VTTPLSPEE253 pKa = 4.02 DD254 pKa = 3.82 TCNHH258 pKa = 6.23 LEE260 pKa = 4.11 WLGPEE265 pKa = 3.91 QKK267 pKa = 10.32 EE268 pKa = 4.01 QFLRR272 pKa = 11.84 RR273 pKa = 11.84 IDD275 pKa = 3.87 HH276 pKa = 6.54 IVRR279 pKa = 11.84 FPQMMTNYY287 pKa = 10.28 LARR290 pKa = 5.36
Molecular weight: 33.32 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 7.111
IPC2_protein 7.439
IPC_protein 7.366
Toseland 7.0
ProMoST 8.039
Dawson 8.039
Bjellqvist 8.375
Wikipedia 7.966
Rodwell 8.053
Grimsley 7.044
Solomon 8.126
Lehninger 8.156
Nozaki 8.609
DTASelect 8.141
Thurlkill 8.2
EMBOSS 8.258
Sillero 8.521
Patrickios 4.228
IPC_peptide 8.126
IPC2_peptide 7.439
IPC2.peptide.svr19 7.552
Protein with the highest isoelectric point:
>tr|A0A0B4UI18|A0A0B4UI18_9VIRU Putative capsid protein OS=Odonata-associated circular virus-18 OX=1592118 PE=4 SV=1
MM1 pKa = 8.13 AYY3 pKa = 10.02 RR4 pKa = 11.84 KK5 pKa = 10.18 KK6 pKa = 9.9 KK7 pKa = 6.98 TFKK10 pKa = 10.17 RR11 pKa = 11.84 KK12 pKa = 9.53 RR13 pKa = 11.84 SFKK16 pKa = 10.39 RR17 pKa = 11.84 PSTRR21 pKa = 11.84 VSKK24 pKa = 10.32 KK25 pKa = 8.39 VKK27 pKa = 10.19 RR28 pKa = 11.84 FVKK31 pKa = 10.21 KK32 pKa = 10.53 YY33 pKa = 9.54 VDD35 pKa = 3.42 RR36 pKa = 11.84 QIEE39 pKa = 4.29 DD40 pKa = 3.22 KK41 pKa = 10.77 RR42 pKa = 11.84 VQTTQTEE49 pKa = 4.52 VTYY52 pKa = 11.39 SNTLTNAQVFSLMPSIGQGVDD73 pKa = 2.95 DD74 pKa = 4.65 SSRR77 pKa = 11.84 TGNVVRR83 pKa = 11.84 LRR85 pKa = 11.84 KK86 pKa = 9.87 AILRR90 pKa = 11.84 LSASAFDD97 pKa = 4.53 NNIHH101 pKa = 6.12 AHH103 pKa = 5.88 WNDD106 pKa = 3.05 LYY108 pKa = 10.75 IFKK111 pKa = 10.36 KK112 pKa = 9.53 QAGIVAPTAAEE123 pKa = 3.97 MNLFLDD129 pKa = 4.1 NGSTAVAYY137 pKa = 9.95 AGQPLGGLRR146 pKa = 11.84 PVNPFTFIKK155 pKa = 10.1 KK156 pKa = 8.17 LHH158 pKa = 6.47 KK159 pKa = 8.53 RR160 pKa = 11.84 TMMASVQSGGASGSAIGSVKK180 pKa = 10.44 PCFTMTKK187 pKa = 10.14 DD188 pKa = 2.78 ITSFFKK194 pKa = 10.85 KK195 pKa = 9.3 RR196 pKa = 11.84 WVYY199 pKa = 10.94 EE200 pKa = 3.97 DD201 pKa = 3.76 STLSTPNNDD210 pKa = 2.82 NVFIGMGGSDD220 pKa = 3.68 MTGQIAANYY229 pKa = 9.32 GEE231 pKa = 4.24 YY232 pKa = 10.15 SYY234 pKa = 11.74 SVDD237 pKa = 4.8 FFYY240 pKa = 11.35 EE241 pKa = 4.08 DD242 pKa = 3.0 AA243 pKa = 5.45
Molecular weight: 27.19 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.143
IPC2_protein 9.458
IPC_protein 9.458
Toseland 10.189
ProMoST 9.809
Dawson 10.365
Bjellqvist 9.999
Wikipedia 10.511
Rodwell 10.891
Grimsley 10.423
Solomon 10.379
Lehninger 10.35
Nozaki 10.16
DTASelect 9.999
Thurlkill 10.218
EMBOSS 10.584
Sillero 10.262
Patrickios 10.555
IPC_peptide 10.379
IPC2_peptide 8.521
IPC2.peptide.svr19 8.476
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
2
0
2
533
243
290
266.5
30.25
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
5.253 ± 1.809
1.501 ± 0.768
4.878 ± 0.333
4.315 ± 1.302
6.567 ± 0.278
7.505 ± 0.359
1.689 ± 0.32
4.503 ± 0.017
7.692 ± 0.96
6.191 ± 0.884
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
3.002 ± 0.205
5.066 ± 0.2
4.503 ± 0.854
4.315 ± 0.431
5.441 ± 0.516
8.255 ± 0.563
7.317 ± 0.354
6.191 ± 0.858
1.876 ± 0.743
3.94 ± 0.124
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here