Mycobacterium phage Konstantine

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; Konstantinevirus; Mycobacterium virus Konstantine

Average proteome isoelectric point is 6.39

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 95 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|B5U524|B5U524_9CAUD Uncharacterized protein OS=Mycobacterium phage Konstantine OX=563121 GN=54 PE=4 SV=1
MM1 pKa = 7.57AAFEE5 pKa = 4.78DD6 pKa = 4.39PEE8 pKa = 5.17LYY10 pKa = 10.8GDD12 pKa = 4.11DD13 pKa = 4.4RR14 pKa = 11.84IVMGSGTDD22 pKa = 3.25QLVITISDD30 pKa = 3.37GGFYY34 pKa = 10.88VVDD37 pKa = 3.58NDD39 pKa = 3.8TGEE42 pKa = 4.0SRR44 pKa = 11.84NYY46 pKa = 10.62YY47 pKa = 9.94SVHH50 pKa = 6.07FLNDD54 pKa = 3.16SFEE57 pKa = 4.44TSVHH61 pKa = 6.39DD62 pKa = 4.0VMGPEE67 pKa = 5.14DD68 pKa = 4.69EE69 pKa = 5.56DD70 pKa = 4.35PNLPEE75 pKa = 4.45ARR77 pKa = 11.84VNVIAMMANWDD88 pKa = 3.88YY89 pKa = 11.14MDD91 pKa = 4.05VSVDD95 pKa = 4.1PIVPKK100 pKa = 10.72YY101 pKa = 11.02DD102 pKa = 3.87FGPEE106 pKa = 3.72VANWCMRR113 pKa = 11.84NEE115 pKa = 4.41DD116 pKa = 3.98EE117 pKa = 6.21LKK119 pKa = 10.73HH120 pKa = 5.37VWNSLVPAA128 pKa = 5.14

Molecular weight: 14.46 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|B5U4Y3|B5U4Y3_9CAUD Uncharacterized protein OS=Mycobacterium phage Konstantine OX=563121 GN=13 PE=4 SV=1
MM1 pKa = 7.09IAARR5 pKa = 11.84KK6 pKa = 7.81PRR8 pKa = 11.84KK9 pKa = 9.25KK10 pKa = 9.83RR11 pKa = 11.84RR12 pKa = 11.84KK13 pKa = 9.94AGTGQSKK20 pKa = 9.9PLTPRR25 pKa = 11.84QQAALKK31 pKa = 9.88IAIRR35 pKa = 11.84AAARR39 pKa = 11.84ARR41 pKa = 11.84KK42 pKa = 8.97LRR44 pKa = 11.84AGTGSVTRR52 pKa = 11.84KK53 pKa = 8.33RR54 pKa = 11.84RR55 pKa = 11.84RR56 pKa = 11.84RR57 pKa = 11.84RR58 pKa = 11.84KK59 pKa = 9.74ARR61 pKa = 3.36

Molecular weight: 6.92 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

95

0

95

21109

28

2107

222.2

24.67

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.044 ± 0.439

0.805 ± 0.124

6.755 ± 0.254

6.604 ± 0.268

3.671 ± 0.191

8.447 ± 0.355

1.838 ± 0.171

5.571 ± 0.182

5.197 ± 0.283

7.21 ± 0.32

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.369 ± 0.187

4.159 ± 0.151

5.187 ± 0.276

3.591 ± 0.207

6.751 ± 0.303

5.614 ± 0.214

5.671 ± 0.159

6.808 ± 0.206

1.904 ± 0.143

2.804 ± 0.221
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski