Thermotoga maritima (strain ATCC 43589 / DSM 3109 / JCM 10099 / NBRC 100826 / MSB8)

Taxonomy: cellular organisms; Bacteria; Thermotogae; Thermotogae; Thermotogales; Thermotogaceae; Thermotoga; Thermotoga maritima

Average proteome isoelectric point is 6.57

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 1852 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|Q9X2G0|Q9X2G0_THEMA Uncharacterized protein OS=Thermotoga maritima (strain ATCC 43589 / DSM 3109 / JCM 10099 / NBRC 100826 / MSB8) OX=243274 GN=TM_1844 PE=4 SV=1
MM1 pKa = 7.57KK2 pKa = 10.58KK3 pKa = 10.37LFLVLAVLSLVLYY16 pKa = 10.2GCLRR20 pKa = 11.84TPPVVKK26 pKa = 10.79DD27 pKa = 3.35MVTTDD32 pKa = 3.79GSLSDD37 pKa = 3.4WGSKK41 pKa = 9.56IKK43 pKa = 10.55EE44 pKa = 4.12DD45 pKa = 4.81AADD48 pKa = 3.95DD49 pKa = 4.41SKK51 pKa = 11.14WGADD55 pKa = 3.51NEE57 pKa = 4.19LLKK60 pKa = 11.11AGLLFDD66 pKa = 4.58GANIYY71 pKa = 10.04IAGEE75 pKa = 3.98YY76 pKa = 10.24VIGGDD81 pKa = 3.55GNVNVFLVLIDD92 pKa = 3.81LVGVTGAQTVSYY104 pKa = 10.07TGLNDD109 pKa = 3.39GNRR112 pKa = 11.84NFTNPNGDD120 pKa = 2.72IDD122 pKa = 6.23LIVEE126 pKa = 4.26VNQSNEE132 pKa = 3.84YY133 pKa = 9.94KK134 pKa = 9.9VWRR137 pKa = 11.84VSQDD141 pKa = 2.66GSLTDD146 pKa = 3.17ITDD149 pKa = 3.2QVTAQFGTGSTIAEE163 pKa = 4.1LKK165 pKa = 10.53IPISEE170 pKa = 4.04QVNQVKK176 pKa = 10.15AVFAISGGTGGGNQWVGDD194 pKa = 4.5FYY196 pKa = 10.99PNQPDD201 pKa = 3.32HH202 pKa = 7.62DD203 pKa = 4.27SAEE206 pKa = 4.33NGGIPQPAPVVNFVVCDD223 pKa = 3.56KK224 pKa = 10.39EE225 pKa = 5.15GNVSEE230 pKa = 5.27EE231 pKa = 4.1INQTSEE237 pKa = 4.14PEE239 pKa = 3.97QPTEE243 pKa = 3.92SVIVVDD249 pKa = 5.33GDD251 pKa = 3.64LSDD254 pKa = 4.58FGTPVATSTNPGGGNGANLYY274 pKa = 9.77RR275 pKa = 11.84LYY277 pKa = 9.86VTYY280 pKa = 10.57DD281 pKa = 2.87ATYY284 pKa = 10.37LYY286 pKa = 10.33IGFDD290 pKa = 3.85TQNSGSWDD298 pKa = 3.31VAYY301 pKa = 10.31GIGIDD306 pKa = 4.14TKK308 pKa = 10.18PSGYY312 pKa = 9.25TGDD315 pKa = 3.35SDD317 pKa = 3.03AWGRR321 pKa = 11.84KK322 pKa = 7.76ISFGADD328 pKa = 3.06YY329 pKa = 11.09AVDD332 pKa = 3.57YY333 pKa = 10.62EE334 pKa = 5.16IYY336 pKa = 9.77FWWAGGSGLDD346 pKa = 4.54SNNFCEE352 pKa = 5.2WNGLDD357 pKa = 3.08WNYY360 pKa = 11.04RR361 pKa = 11.84SIADD365 pKa = 3.3AGGTFDD371 pKa = 3.69YY372 pKa = 11.09TGDD375 pKa = 3.74TSSGLQTMEE384 pKa = 4.46IAIPWSAIGGKK395 pKa = 9.28QDD397 pKa = 2.76VALIVWITGGSGSAVDD413 pKa = 5.69SIPDD417 pKa = 3.98DD418 pKa = 5.22DD419 pKa = 4.28STIDD423 pKa = 6.1NADD426 pKa = 3.13EE427 pKa = 4.36WNDD430 pKa = 3.09SDD432 pKa = 4.81TFTNLYY438 pKa = 9.9LLQVNN443 pKa = 4.57

Molecular weight: 47.42 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|Q9WXM0|Q9WXM0_THEMA DNA helicase putative OS=Thermotoga maritima (strain ATCC 43589 / DSM 3109 / JCM 10099 / NBRC 100826 / MSB8) OX=243274 GN=TM_0005 PE=3 SV=1
MM1 pKa = 7.35KK2 pKa = 9.4RR3 pKa = 11.84TYY5 pKa = 10.16QPSRR9 pKa = 11.84RR10 pKa = 11.84KK11 pKa = 9.66RR12 pKa = 11.84KK13 pKa = 8.13RR14 pKa = 11.84THH16 pKa = 6.11GFLARR21 pKa = 11.84KK22 pKa = 6.6RR23 pKa = 11.84TPGGRR28 pKa = 11.84RR29 pKa = 11.84VLKK32 pKa = 9.72NRR34 pKa = 11.84RR35 pKa = 11.84RR36 pKa = 11.84KK37 pKa = 9.65GRR39 pKa = 11.84WRR41 pKa = 11.84LTVV44 pKa = 3.0

Molecular weight: 5.5 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

1852

0

1852

582801

30

1690

314.7

35.72

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.849 ± 0.051

0.708 ± 0.016

4.966 ± 0.036

8.924 ± 0.065

5.195 ± 0.044

6.908 ± 0.045

1.585 ± 0.019

7.185 ± 0.037

7.617 ± 0.051

10.039 ± 0.06

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.403 ± 0.023

3.61 ± 0.031

3.993 ± 0.033

2.013 ± 0.026

5.536 ± 0.048

5.651 ± 0.045

4.526 ± 0.031

8.61 ± 0.052

1.102 ± 0.022

3.58 ± 0.035
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski