Vitis vinifera (Grape)

Taxonomy: cellular organisms; Eukaryota; Viridiplantae; Streptophyta; Streptophytina; Embryophyta; Tracheophyta; Euphyllophyta; Spermatophyta; Magnoliopsida; Mesangiospermae; eudicotyledons; Gunneridae; Pentapetalae; rosids; rosids incertae sedis; Vitales; Vitaceae; Viteae; Vitis

Average proteome isoelectric point is 6.78

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 29909 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|F6HA46|F6HA46_VITVI Uncharacterized protein OS=Vitis vinifera OX=29760 GN=VIT_06s0009g03310 PE=4 SV=1
MM1 pKa = 6.95TRR3 pKa = 11.84SGRR6 pKa = 11.84IAQAAPPFTRR16 pKa = 11.84PFGVQSEE23 pKa = 4.73SFSPLEE29 pKa = 3.86LFGVSIIKK37 pKa = 9.99IAEE40 pKa = 3.95EE41 pKa = 4.25DD42 pKa = 3.45QTIPAPKK49 pKa = 9.99LPVFVVPTTDD59 pKa = 3.14MYY61 pKa = 11.25EE62 pKa = 4.09STIGLVEE69 pKa = 4.23GVSDD73 pKa = 5.2FMDD76 pKa = 4.5PSLSFDD82 pKa = 3.18ILSGFVTHH90 pKa = 6.94SDD92 pKa = 3.45YY93 pKa = 11.61VYY95 pKa = 10.8DD96 pKa = 4.72DD97 pKa = 3.77SVMDD101 pKa = 3.94LSIYY105 pKa = 10.34EE106 pKa = 4.23YY107 pKa = 11.19LSVSYY112 pKa = 11.06DD113 pKa = 3.41DD114 pKa = 3.9VSFFIMNLIPP124 pKa = 4.75

Molecular weight: 13.76 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|F6I4S5|F6I4S5_VITVI Uncharacterized protein OS=Vitis vinifera OX=29760 GN=VIT_14s0060g01220 PE=4 SV=1
MM1 pKa = 7.42RR2 pKa = 11.84RR3 pKa = 11.84RR4 pKa = 11.84WRR6 pKa = 11.84WWWGRR11 pKa = 11.84FVVVWMRR18 pKa = 11.84RR19 pKa = 11.84RR20 pKa = 11.84WGWWRR25 pKa = 11.84WRR27 pKa = 11.84LVVVWRR33 pKa = 4.42

Molecular weight: 4.83 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

29874

35

29909

10773495

8

5063

360.2

40.17

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.685 ± 0.015

1.911 ± 0.008

5.113 ± 0.008

6.407 ± 0.014

4.311 ± 0.009

6.762 ± 0.015

2.438 ± 0.006

5.409 ± 0.012

5.941 ± 0.014

10.014 ± 0.019

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.537 ± 0.006

4.286 ± 0.01

4.973 ± 0.013

3.632 ± 0.01

5.247 ± 0.012

8.824 ± 0.016

4.803 ± 0.01

6.493 ± 0.01

1.365 ± 0.005

2.785 ± 0.008
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski