Salmonella phage SETP13
Average proteome isoelectric point is 6.44
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 69 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|U5N0W5|U5N0W5_9CAUD Putative tail tape measure protein OS=Salmonella phage SETP13 OX=424949 PE=4 SV=1
MM1 pKa = 7.73 PMLIVAALVTIAFAIGLILLFIIMDD26 pKa = 3.87 ASVFVNVYY34 pKa = 10.43 KK35 pKa = 11.15 GITGLTAVEE44 pKa = 3.96 CRR46 pKa = 11.84 ILYY49 pKa = 8.09 VGCVAVSAAVLLGEE63 pKa = 5.46 DD64 pKa = 4.17 LDD66 pKa = 4.27 LL67 pKa = 6.22
Molecular weight: 7.03 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.938
IPC2_protein 4.24
IPC_protein 3.923
Toseland 3.757
ProMoST 4.139
Dawson 3.935
Bjellqvist 4.101
Wikipedia 3.91
Rodwell 3.783
Grimsley 3.694
Solomon 3.884
Lehninger 3.846
Nozaki 4.101
DTASelect 4.253
Thurlkill 3.859
EMBOSS 3.923
Sillero 4.05
Patrickios 0.477
IPC_peptide 3.884
IPC2_peptide 4.024
IPC2.peptide.svr19 3.963
Protein with the highest isoelectric point:
>tr|U5N3W8|U5N3W8_9CAUD Uncharacterized protein OS=Salmonella phage SETP13 OX=424949 PE=4 SV=1
MM1 pKa = 7.09 SLAYY5 pKa = 9.92 RR6 pKa = 11.84 RR7 pKa = 11.84 LNAHH11 pKa = 6.64 KK12 pKa = 10.18 AGVVTSMRR20 pKa = 11.84 LFVMWYY26 pKa = 9.17 AEE28 pKa = 4.31 TVFGRR33 pKa = 11.84 RR34 pKa = 11.84 FYY36 pKa = 10.78 AIHH39 pKa = 5.65 SRR41 pKa = 11.84 GWRR44 pKa = 11.84 WEE46 pKa = 4.07 GVGKK50 pKa = 9.11 WWVYY54 pKa = 11.38 KK55 pKa = 10.65 DD56 pKa = 3.68 GQDD59 pKa = 3.16
Molecular weight: 7.13 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.295
IPC2_protein 9.94
IPC_protein 10.687
Toseland 10.394
ProMoST 10.204
Dawson 10.613
Bjellqvist 10.35
Wikipedia 10.847
Rodwell 10.789
Grimsley 10.701
Solomon 10.687
Lehninger 10.643
Nozaki 10.365
DTASelect 10.35
Thurlkill 10.452
EMBOSS 10.804
Sillero 10.526
Patrickios 10.54
IPC_peptide 10.672
IPC2_peptide 9.253
IPC2.peptide.svr19 8.343
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
69
0
69
13101
47
852
189.9
21.05
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
9.9 ± 0.583
1.0 ± 0.127
6.206 ± 0.228
6.786 ± 0.431
3.832 ± 0.138
7.664 ± 0.351
1.672 ± 0.182
4.877 ± 0.203
6.068 ± 0.34
7.717 ± 0.241
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.58 ± 0.171
4.274 ± 0.211
3.855 ± 0.226
3.595 ± 0.254
5.648 ± 0.224
5.755 ± 0.304
6.099 ± 0.306
7.511 ± 0.294
1.443 ± 0.155
3.519 ± 0.184
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here