Pseudomonas sp. MF4836

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Gammaproteobacteria; Pseudomonadales; Pseudomonadaceae; Pseudomonas; unclassified Pseudomonas

Average proteome isoelectric point is 6.42

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 6768 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1T1I5I3|A0A1T1I5I3_9PSED Uncharacterized protein OS=Pseudomonas sp. MF4836 OX=1960827 GN=MF4836_11260 PE=4 SV=1
MM1 pKa = 7.53SNDD4 pKa = 3.24EE5 pKa = 4.32NQVHH9 pKa = 5.4VWIGSNFAPEE19 pKa = 4.22DD20 pKa = 3.23QYY22 pKa = 11.5MEE24 pKa = 4.36YY25 pKa = 10.53FQLDD29 pKa = 3.79YY30 pKa = 11.2SVEE33 pKa = 4.09GDD35 pKa = 4.33FDD37 pKa = 4.28DD38 pKa = 5.81PSYY41 pKa = 11.0KK42 pKa = 10.65LCGFCEE48 pKa = 4.85DD49 pKa = 5.85IGTQWYY55 pKa = 10.04DD56 pKa = 2.85EE57 pKa = 4.46DD58 pKa = 5.06FIGIIPRR65 pKa = 11.84SDD67 pKa = 3.58AEE69 pKa = 4.15VSLDD73 pKa = 4.6EE74 pKa = 4.55ILQEE78 pKa = 4.09AAVDD82 pKa = 3.83QDD84 pKa = 3.3QLDD87 pKa = 3.83AVKK90 pKa = 10.24QRR92 pKa = 11.84CAALGITQANAIFWYY107 pKa = 10.29QDD109 pKa = 2.54ADD111 pKa = 3.98LVLKK115 pKa = 10.43QPIKK119 pKa = 10.59DD120 pKa = 3.34QYY122 pKa = 11.76NGLKK126 pKa = 10.66YY127 pKa = 10.3IGLFKK132 pKa = 11.11GDD134 pKa = 3.37

Molecular weight: 15.34 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1T1I4Z9|A0A1T1I4Z9_9PSED Uncharacterized protein OS=Pseudomonas sp. MF4836 OX=1960827 GN=MF4836_10790 PE=4 SV=1
MM1 pKa = 7.45KK2 pKa = 9.56RR3 pKa = 11.84TFQPSTIKK11 pKa = 10.52RR12 pKa = 11.84ARR14 pKa = 11.84THH16 pKa = 5.94GFRR19 pKa = 11.84ARR21 pKa = 11.84MATKK25 pKa = 10.16NGRR28 pKa = 11.84AVLSRR33 pKa = 11.84RR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 10.38GRR39 pKa = 11.84ARR41 pKa = 11.84LAVV44 pKa = 3.42

Molecular weight: 5.07 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

6768

0

6768

2209861

23

7597

326.5

35.78

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

11.068 ± 0.039

0.984 ± 0.011

5.238 ± 0.027

5.502 ± 0.033

3.654 ± 0.02

7.98 ± 0.035

2.271 ± 0.015

4.743 ± 0.024

3.347 ± 0.032

12.022 ± 0.051

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.228 ± 0.015

3.034 ± 0.027

4.893 ± 0.022

4.88 ± 0.026

6.296 ± 0.037

6.074 ± 0.028

4.778 ± 0.037

6.949 ± 0.026

1.466 ± 0.013

2.594 ± 0.014
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski