Amino acid dipepetide frequency for Microbacterium humi

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
19.272AlaAla: 19.272 ± 0.223
0.729AlaCys: 0.729 ± 0.024
7.934AlaAsp: 7.934 ± 0.101
8.009AlaGlu: 8.009 ± 0.109
3.913AlaPhe: 3.913 ± 0.067
11.337AlaGly: 11.337 ± 0.119
2.512AlaHis: 2.512 ± 0.06
6.088AlaIle: 6.088 ± 0.089
3.103AlaLys: 3.103 ± 0.065
13.41AlaLeu: 13.41 ± 0.15
2.784AlaMet: 2.784 ± 0.055
2.531AlaAsn: 2.531 ± 0.049
5.596AlaPro: 5.596 ± 0.091
3.647AlaGln: 3.647 ± 0.056
8.755AlaArg: 8.755 ± 0.116
7.573AlaSer: 7.573 ± 0.091
6.979AlaThr: 6.979 ± 0.084
11.097AlaVal: 11.097 ± 0.125
1.793AlaTrp: 1.793 ± 0.04
2.283AlaTyr: 2.283 ± 0.047
0.0AlaXaa: 0.0 ± 0.0
Cys
0.71CysAla: 0.71 ± 0.025
0.058CysCys: 0.058 ± 0.007
0.356CysAsp: 0.356 ± 0.019
0.297CysGlu: 0.297 ± 0.018
0.184CysPhe: 0.184 ± 0.015
0.633CysGly: 0.633 ± 0.024
0.116CysHis: 0.116 ± 0.01
0.253CysIle: 0.253 ± 0.017
0.073CysLys: 0.073 ± 0.008
0.465CysLeu: 0.465 ± 0.022
0.075CysMet: 0.075 ± 0.008
0.116CysAsn: 0.116 ± 0.011
0.275CysPro: 0.275 ± 0.02
0.116CysGln: 0.116 ± 0.012
0.393CysArg: 0.393 ± 0.02
0.393CysSer: 0.393 ± 0.021
0.36CysThr: 0.36 ± 0.02
0.455CysVal: 0.455 ± 0.02
0.075CysTrp: 0.075 ± 0.009
0.116CysTyr: 0.116 ± 0.01
0.0CysXaa: 0.0 ± 0.0
Asp
9.521AspAla: 9.521 ± 0.113
0.259AspCys: 0.259 ± 0.016
4.5AspAsp: 4.5 ± 0.082
4.784AspGlu: 4.784 ± 0.084
1.797AspPhe: 1.797 ± 0.042
5.676AspGly: 5.676 ± 0.091
1.101AspHis: 1.101 ± 0.033
2.662AspIle: 2.662 ± 0.061
1.312AspLys: 1.312 ± 0.042
5.581AspLeu: 5.581 ± 0.064
0.92AspMet: 0.92 ± 0.032
1.111AspAsn: 1.111 ± 0.034
3.642AspPro: 3.642 ± 0.063
1.5AspGln: 1.5 ± 0.037
4.091AspArg: 4.091 ± 0.07
2.945AspSer: 2.945 ± 0.052
3.036AspThr: 3.036 ± 0.053
5.672AspVal: 5.672 ± 0.082
0.955AspTrp: 0.955 ± 0.035
1.4AspTyr: 1.4 ± 0.041
0.0AspXaa: 0.0 ± 0.0
Glu
6.67GluAla: 6.67 ± 0.093
0.28GluCys: 0.28 ± 0.019
2.713GluAsp: 2.713 ± 0.051
2.862GluGlu: 2.862 ± 0.056
1.814GluPhe: 1.814 ± 0.041
3.852GluGly: 3.852 ± 0.066
1.613GluHis: 1.613 ± 0.044
2.843GluIle: 2.843 ± 0.061
1.784GluLys: 1.784 ± 0.05
6.94GluLeu: 6.94 ± 0.087
0.981GluMet: 0.981 ± 0.034
1.543GluAsn: 1.543 ± 0.04
3.083GluPro: 3.083 ± 0.054
2.474GluGln: 2.474 ± 0.048
5.536GluArg: 5.536 ± 0.091
3.252GluSer: 3.252 ± 0.063
3.099GluThr: 3.099 ± 0.057
4.096GluVal: 4.096 ± 0.065
0.833GluTrp: 0.833 ± 0.033
1.199GluTyr: 1.199 ± 0.031
0.0GluXaa: 0.0 ± 0.0
Phe
4.34PheAla: 4.34 ± 0.071
0.189PheCys: 0.189 ± 0.013
2.417PheAsp: 2.417 ± 0.059
1.892PheGlu: 1.892 ± 0.041
1.088PhePhe: 1.088 ± 0.039
3.403PheGly: 3.403 ± 0.069
0.568PheHis: 0.568 ± 0.022
1.37PheIle: 1.37 ± 0.042
0.526PheLys: 0.526 ± 0.022
2.72PheLeu: 2.72 ± 0.055
0.512PheMet: 0.512 ± 0.018
0.761PheAsn: 0.761 ± 0.032
1.461PhePro: 1.461 ± 0.043
0.75PheGln: 0.75 ± 0.027
1.781PheArg: 1.781 ± 0.042
1.999PheSer: 1.999 ± 0.043
2.182PheThr: 2.182 ± 0.05
2.911PheVal: 2.911 ± 0.057
0.464PheTrp: 0.464 ± 0.023
0.742PheTyr: 0.742 ± 0.026
0.0PheXaa: 0.0 ± 0.0
Gly
9.801GlyAla: 9.801 ± 0.109
0.609GlyCys: 0.609 ± 0.026
4.982GlyAsp: 4.982 ± 0.073
4.877GlyGlu: 4.877 ± 0.07
3.266GlyPhe: 3.266 ± 0.054
6.999GlyGly: 6.999 ± 0.107
1.821GlyHis: 1.821 ± 0.044
4.963GlyIle: 4.963 ± 0.076
2.353GlyLys: 2.353 ± 0.054
8.627GlyLeu: 8.627 ± 0.106
1.92GlyMet: 1.92 ± 0.044
1.827GlyAsn: 1.827 ± 0.047
3.399GlyPro: 3.399 ± 0.059
2.468GlyGln: 2.468 ± 0.046
6.016GlyArg: 6.016 ± 0.084
5.442GlySer: 5.442 ± 0.072
5.339GlyThr: 5.339 ± 0.073
7.674GlyVal: 7.674 ± 0.094
1.476GlyTrp: 1.476 ± 0.045
2.222GlyTyr: 2.222 ± 0.053
0.0GlyXaa: 0.0 ± 0.0
His
2.426HisAla: 2.426 ± 0.051
0.124HisCys: 0.124 ± 0.01
1.375HisAsp: 1.375 ± 0.034
1.278HisGlu: 1.278 ± 0.037
0.546HisPhe: 0.546 ± 0.023
1.961HisGly: 1.961 ± 0.047
0.526HisHis: 0.526 ± 0.026
0.819HisIle: 0.819 ± 0.026
0.381HisLys: 0.381 ± 0.02
1.851HisLeu: 1.851 ± 0.046
0.339HisMet: 0.339 ± 0.019
0.438HisAsn: 0.438 ± 0.02
1.415HisPro: 1.415 ± 0.044
0.468HisGln: 0.468 ± 0.021
1.508HisArg: 1.508 ± 0.041
1.097HisSer: 1.097 ± 0.037
1.094HisThr: 1.094 ± 0.035
1.873HisVal: 1.873 ± 0.045
0.297HisTrp: 0.297 ± 0.018
0.444HisTyr: 0.444 ± 0.023
0.0HisXaa: 0.0 ± 0.0
Ile
7.293IleAla: 7.293 ± 0.105
0.259IleCys: 0.259 ± 0.015
3.64IleAsp: 3.64 ± 0.062
3.146IleGlu: 3.146 ± 0.062
1.277IlePhe: 1.277 ± 0.04
4.996IleGly: 4.996 ± 0.076
0.696IleHis: 0.696 ± 0.028
2.219IleIle: 2.219 ± 0.05
0.876IleLys: 0.876 ± 0.035
3.834IleLeu: 3.834 ± 0.07
0.746IleMet: 0.746 ± 0.027
1.025IleAsn: 1.025 ± 0.037
2.466IlePro: 2.466 ± 0.05
0.942IleGln: 0.942 ± 0.037
2.811IleArg: 2.811 ± 0.054
2.627IleSer: 2.627 ± 0.06
2.962IleThr: 2.962 ± 0.059
5.18IleVal: 5.18 ± 0.085
0.565IleTrp: 0.565 ± 0.024
0.769IleTyr: 0.769 ± 0.03
0.0IleXaa: 0.0 ± 0.0
Lys
2.74LysAla: 2.74 ± 0.06
0.079LysCys: 0.079 ± 0.009
1.349LysAsp: 1.349 ± 0.048
0.994LysGlu: 0.994 ± 0.036
0.564LysPhe: 0.564 ± 0.024
1.816LysGly: 1.816 ± 0.05
0.581LysHis: 0.581 ± 0.025
1.04LysIle: 1.04 ± 0.033
1.015LysLys: 1.015 ± 0.044
2.289LysLeu: 2.289 ± 0.054
0.45LysMet: 0.45 ± 0.023
0.675LysAsn: 0.675 ± 0.026
1.451LysPro: 1.451 ± 0.045
0.892LysGln: 0.892 ± 0.033
2.005LysArg: 2.005 ± 0.042
1.443LysSer: 1.443 ± 0.041
1.539LysThr: 1.539 ± 0.046
1.745LysVal: 1.745 ± 0.047
0.278LysTrp: 0.278 ± 0.017
0.503LysTyr: 0.503 ± 0.023
0.0LysXaa: 0.0 ± 0.0
Leu
13.934LeuAla: 13.934 ± 0.131
0.63LeuCys: 0.63 ± 0.02
6.754LeuAsp: 6.754 ± 0.092
5.58LeuGlu: 5.58 ± 0.086
2.927LeuPhe: 2.927 ± 0.069
9.017LeuGly: 9.017 ± 0.105
1.877LeuHis: 1.877 ± 0.049
4.647LeuIle: 4.647 ± 0.084
2.104LeuLys: 2.104 ± 0.048
10.042LeuLeu: 10.042 ± 0.115
1.689LeuMet: 1.689 ± 0.043
1.973LeuAsn: 1.973 ± 0.047
5.323LeuPro: 5.323 ± 0.074
2.446LeuGln: 2.446 ± 0.052
7.123LeuArg: 7.123 ± 0.093
6.281LeuSer: 6.281 ± 0.079
6.282LeuThr: 6.282 ± 0.076
9.135LeuVal: 9.135 ± 0.106
1.248LeuTrp: 1.248 ± 0.039
1.821LeuTyr: 1.821 ± 0.052
0.0LeuXaa: 0.0 ± 0.0
Met
2.272MetAla: 2.272 ± 0.043
0.107MetCys: 0.107 ± 0.01
0.779MetAsp: 0.779 ± 0.03
0.643MetGlu: 0.643 ± 0.026
0.582MetPhe: 0.582 ± 0.027
1.307MetGly: 1.307 ± 0.037
0.353MetHis: 0.353 ± 0.017
1.005MetIle: 1.005 ± 0.031
0.451MetLys: 0.451 ± 0.021
2.091MetLeu: 2.091 ± 0.047
0.361MetMet: 0.361 ± 0.023
0.547MetAsn: 0.547 ± 0.024
1.234MetPro: 1.234 ± 0.036
0.538MetGln: 0.538 ± 0.023
1.412MetArg: 1.412 ± 0.039
1.592MetSer: 1.592 ± 0.04
1.785MetThr: 1.785 ± 0.04
1.375MetVal: 1.375 ± 0.037
0.23MetTrp: 0.23 ± 0.017
0.281MetTyr: 0.281 ± 0.018
0.0MetXaa: 0.0 ± 0.0
Asn
2.752AsnAla: 2.752 ± 0.057
0.132AsnCys: 0.132 ± 0.012
1.279AsnAsp: 1.279 ± 0.038
1.17AsnGlu: 1.17 ± 0.036
0.67AsnPhe: 0.67 ± 0.028
2.211AsnGly: 2.211 ± 0.046
0.41AsnHis: 0.41 ± 0.023
0.993AsnIle: 0.993 ± 0.034
0.493AsnLys: 0.493 ± 0.024
2.039AsnLeu: 2.039 ± 0.05
0.387AsnMet: 0.387 ± 0.021
0.512AsnAsn: 0.512 ± 0.025
1.624AsnPro: 1.624 ± 0.041
0.628AsnGln: 0.628 ± 0.027
1.429AsnArg: 1.429 ± 0.042
1.175AsnSer: 1.175 ± 0.035
1.185AsnThr: 1.185 ± 0.037
1.937AsnVal: 1.937 ± 0.046
0.334AsnTrp: 0.334 ± 0.017
0.556AsnTyr: 0.556 ± 0.027
0.0AsnXaa: 0.0 ± 0.0
Pro
6.403ProAla: 6.403 ± 0.096
0.202ProCys: 0.202 ± 0.015
3.82ProAsp: 3.82 ± 0.071
3.724ProGlu: 3.724 ± 0.07
1.7ProPhe: 1.7 ± 0.039
4.573ProGly: 4.573 ± 0.073
1.07ProHis: 1.07 ± 0.032
2.022ProIle: 2.022 ± 0.042
1.179ProLys: 1.179 ± 0.037
4.804ProLeu: 4.804 ± 0.073
0.924ProMet: 0.924 ± 0.031
1.144ProAsn: 1.144 ± 0.032
1.938ProPro: 1.938 ± 0.053
1.584ProGln: 1.584 ± 0.049
3.187ProArg: 3.187 ± 0.063
3.148ProSer: 3.148 ± 0.057
3.012ProThr: 3.012 ± 0.06
4.61ProVal: 4.61 ± 0.069
0.816ProTrp: 0.816 ± 0.029
1.114ProTyr: 1.114 ± 0.034
0.0ProXaa: 0.0 ± 0.0
Gln
3.277GlnAla: 3.277 ± 0.054
0.119GlnCys: 0.119 ± 0.01
1.242GlnAsp: 1.242 ± 0.034
1.202GlnGlu: 1.202 ± 0.035
0.943GlnPhe: 0.943 ± 0.031
1.93GlnGly: 1.93 ± 0.044
0.683GlnHis: 0.683 ± 0.028
1.479GlnIle: 1.479 ± 0.04
0.921GlnLys: 0.921 ± 0.026
3.438GlnLeu: 3.438 ± 0.064
0.596GlnMet: 0.596 ± 0.024
0.727GlnAsn: 0.727 ± 0.026
1.6GlnPro: 1.6 ± 0.047
1.202GlnGln: 1.202 ± 0.039
2.43GlnArg: 2.43 ± 0.045
1.758GlnSer: 1.758 ± 0.046
1.488GlnThr: 1.488 ± 0.04
2.154GlnVal: 2.154 ± 0.05
0.414GlnTrp: 0.414 ± 0.021
0.658GlnTyr: 0.658 ± 0.027
0.0GlnXaa: 0.0 ± 0.0
Arg
8.076ArgAla: 8.076 ± 0.114
0.311ArgCys: 0.311 ± 0.02
4.335ArgAsp: 4.335 ± 0.066
4.635ArgGlu: 4.635 ± 0.077
2.576ArgPhe: 2.576 ± 0.046
5.084ArgGly: 5.084 ± 0.066
1.663ArgHis: 1.663 ± 0.046
3.647ArgIle: 3.647 ± 0.061
1.534ArgLys: 1.534 ± 0.039
7.299ArgLeu: 7.299 ± 0.098
1.717ArgMet: 1.717 ± 0.045
1.49ArgAsn: 1.49 ± 0.041
3.346ArgPro: 3.346 ± 0.064
2.089ArgGln: 2.089 ± 0.044
6.392ArgArg: 6.392 ± 0.095
4.375ArgSer: 4.375 ± 0.074
4.109ArgThr: 4.109 ± 0.065
6.008ArgVal: 6.008 ± 0.088
1.134ArgTrp: 1.134 ± 0.035
1.682ArgTyr: 1.682 ± 0.038
0.0ArgXaa: 0.0 ± 0.0
Ser
7.436SerAla: 7.436 ± 0.098
0.333SerCys: 0.333 ± 0.018
3.594SerAsp: 3.594 ± 0.062
3.096SerGlu: 3.096 ± 0.061
2.104SerPhe: 2.104 ± 0.046
5.797SerGly: 5.797 ± 0.083
1.143SerHis: 1.143 ± 0.035
2.892SerIle: 2.892 ± 0.053
1.365SerLys: 1.365 ± 0.042
5.827SerLeu: 5.827 ± 0.078
1.289SerMet: 1.289 ± 0.036
1.295SerAsn: 1.295 ± 0.041
3.12SerPro: 3.12 ± 0.06
1.626SerGln: 1.626 ± 0.045
4.118SerArg: 4.118 ± 0.064
3.907SerSer: 3.907 ± 0.081
3.754SerThr: 3.754 ± 0.075
5.083SerVal: 5.083 ± 0.076
0.965SerTrp: 0.965 ± 0.032
1.282SerTyr: 1.282 ± 0.037
0.0SerXaa: 0.0 ± 0.0
Thr
7.649ThrAla: 7.649 ± 0.098
0.29ThrCys: 0.29 ± 0.017
3.588ThrAsp: 3.588 ± 0.059
3.069ThrGlu: 3.069 ± 0.05
1.829ThrPhe: 1.829 ± 0.042
5.727ThrGly: 5.727 ± 0.08
1.12ThrHis: 1.12 ± 0.038
3.087ThrIle: 3.087 ± 0.065
1.383ThrLys: 1.383 ± 0.041
5.903ThrLeu: 5.903 ± 0.086
1.063ThrMet: 1.063 ± 0.028
1.224ThrAsn: 1.224 ± 0.038
3.596ThrPro: 3.596 ± 0.057
1.47ThrGln: 1.47 ± 0.04
3.865ThrArg: 3.865 ± 0.065
3.483ThrSer: 3.483 ± 0.064
3.681ThrThr: 3.681 ± 0.079
5.736ThrVal: 5.736 ± 0.078
0.872ThrTrp: 0.872 ± 0.03
1.112ThrTyr: 1.112 ± 0.036
0.0ThrXaa: 0.0 ± 0.0
Val
10.765ValAla: 10.765 ± 0.115
0.589ValCys: 0.589 ± 0.029
5.716ValAsp: 5.716 ± 0.082
4.467ValGlu: 4.467 ± 0.074
2.92ValPhe: 2.92 ± 0.053
6.797ValGly: 6.797 ± 0.091
1.747ValHis: 1.747 ± 0.039
4.792ValIle: 4.792 ± 0.079
1.821ValLys: 1.821 ± 0.047
9.565ValLeu: 9.565 ± 0.122
1.561ValMet: 1.561 ± 0.04
2.014ValAsn: 2.014 ± 0.056
4.655ValPro: 4.655 ± 0.07
2.202ValGln: 2.202 ± 0.049
5.828ValArg: 5.828 ± 0.08
5.381ValSer: 5.381 ± 0.07
5.885ValThr: 5.885 ± 0.082
8.134ValVal: 8.134 ± 0.112
1.145ValTrp: 1.145 ± 0.038
1.613ValTyr: 1.613 ± 0.042
0.0ValXaa: 0.0 ± 0.0
Trp
1.486TrpAla: 1.486 ± 0.04
0.095TrpCys: 0.095 ± 0.009
0.749TrpAsp: 0.749 ± 0.029
0.628TrpGlu: 0.628 ± 0.022
0.588TrpPhe: 0.588 ± 0.028
1.051TrpGly: 1.051 ± 0.035
0.342TrpHis: 0.342 ± 0.018
0.712TrpIle: 0.712 ± 0.028
0.342TrpLys: 0.342 ± 0.018
1.877TrpLeu: 1.877 ± 0.048
0.321TrpMet: 0.321 ± 0.018
0.471TrpAsn: 0.471 ± 0.021
0.688TrpPro: 0.688 ± 0.027
0.609TrpGln: 0.609 ± 0.025
1.186TrpArg: 1.186 ± 0.035
0.931TrpSer: 0.931 ± 0.031
0.825TrpThr: 0.825 ± 0.033
1.052TrpVal: 1.052 ± 0.034
0.374TrpTrp: 0.374 ± 0.022
0.295TrpTyr: 0.295 ± 0.017
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.523TyrAla: 2.523 ± 0.051
0.124TyrCys: 0.124 ± 0.011
1.341TyrAsp: 1.341 ± 0.034
1.114TyrGlu: 1.114 ± 0.033
0.787TyrPhe: 0.787 ± 0.029
1.917TyrGly: 1.917 ± 0.045
0.317TyrHis: 0.317 ± 0.02
0.796TyrIle: 0.796 ± 0.029
0.431TyrLys: 0.431 ± 0.022
2.23TyrLeu: 2.23 ± 0.046
0.312TyrMet: 0.312 ± 0.016
0.489TyrAsn: 0.489 ± 0.026
1.064TyrPro: 1.064 ± 0.034
0.611TyrGln: 0.611 ± 0.027
1.633TyrArg: 1.633 ± 0.044
1.224TyrSer: 1.224 ± 0.034
1.151TyrThr: 1.151 ± 0.038
1.674TyrVal: 1.674 ± 0.043
0.354TyrTrp: 0.354 ± 0.019
0.501TyrTyr: 0.501 ± 0.025
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 3206 proteins (1016218 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski