Gammapapillomavirus 25
Average proteome isoelectric point is 6.17
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 7 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A2D2AMD9|A0A2D2AMD9_9PAPI Minor capsid protein L2 OS=Gammapapillomavirus 25 OX=1961682 GN=L2 PE=3 SV=1
MM1 pKa = 7.67 IGPSPNNDD9 pKa = 4.03 DD10 pKa = 5.03 IEE12 pKa = 6.57 LDD14 pKa = 3.5 LHH16 pKa = 7.27 DD17 pKa = 5.77 LVLPSNLISEE27 pKa = 4.91 EE28 pKa = 4.12 SLSPDD33 pKa = 2.79 VDD35 pKa = 3.83 PEE37 pKa = 4.19 EE38 pKa = 5.06 EE39 pKa = 4.16 EE40 pKa = 3.95 QLPFAVEE47 pKa = 4.53 TSCKK51 pKa = 10.04 SCGAGVRR58 pKa = 11.84 FTVCATHH65 pKa = 6.78 AAIRR69 pKa = 11.84 TLQILLLQEE78 pKa = 4.58 LSLICLRR85 pKa = 11.84 CSRR88 pKa = 11.84 SLIQHH93 pKa = 6.13 GRR95 pKa = 11.84 PHH97 pKa = 6.63
Molecular weight: 10.65 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.492
IPC2_protein 4.622
IPC_protein 4.495
Toseland 4.368
ProMoST 4.571
Dawson 4.444
Bjellqvist 4.596
Wikipedia 4.317
Rodwell 4.355
Grimsley 4.279
Solomon 4.444
Lehninger 4.393
Nozaki 4.571
DTASelect 4.698
Thurlkill 4.368
EMBOSS 4.342
Sillero 4.622
Patrickios 3.35
IPC_peptide 4.457
IPC2_peptide 4.609
IPC2.peptide.svr19 4.567
Protein with the highest isoelectric point:
>tr|A0A2D2AMB7|A0A2D2AMB7_9PAPI E4 OS=Gammapapillomavirus 25 OX=1961682 GN=E4 PE=4 SV=1
MM1 pKa = 7.0 NQADD5 pKa = 3.68 LTARR9 pKa = 11.84 FDD11 pKa = 3.97 ALQEE15 pKa = 4.17 TLMTLYY21 pKa = 10.29 EE22 pKa = 4.17 RR23 pKa = 11.84 NARR26 pKa = 11.84 DD27 pKa = 3.6 LEE29 pKa = 4.26 SQILHH34 pKa = 6.42 WEE36 pKa = 4.49 TVRR39 pKa = 11.84 KK40 pKa = 9.93 QYY42 pKa = 9.59 VTMYY46 pKa = 9.06 YY47 pKa = 10.43 ARR49 pKa = 11.84 KK50 pKa = 9.55 EE51 pKa = 4.26 GFRR54 pKa = 11.84 SLGMQPLPALTVSEE68 pKa = 4.51 YY69 pKa = 10.11 KK70 pKa = 10.66 AKK72 pKa = 10.74 EE73 pKa = 4.35 GIQMVLLLRR82 pKa = 11.84 SLQKK86 pKa = 10.62 SEE88 pKa = 4.43 FATEE92 pKa = 3.87 DD93 pKa = 2.73 WTLIDD98 pKa = 3.6 TSAEE102 pKa = 4.0 LLHH105 pKa = 6.36 TPPKK109 pKa = 10.58 NCFKK113 pKa = 10.85 KK114 pKa = 10.39 GGYY117 pKa = 6.19 TVEE120 pKa = 4.35 VYY122 pKa = 10.61 FDD124 pKa = 4.13 EE125 pKa = 5.66 NPDD128 pKa = 3.42 NVFPYY133 pKa = 9.85 TNWDD137 pKa = 4.19 FIYY140 pKa = 11.08 YY141 pKa = 9.94 QDD143 pKa = 5.04 TNEE146 pKa = 4.34 KK147 pKa = 6.92 WHH149 pKa = 6.06 KK150 pKa = 8.62 TAGLVDD156 pKa = 3.78 YY157 pKa = 10.94 DD158 pKa = 3.5 GMYY161 pKa = 10.32 FQEE164 pKa = 4.53 PNGDD168 pKa = 2.88 RR169 pKa = 11.84 TYY171 pKa = 11.1 FQLFEE176 pKa = 5.05 KK177 pKa = 10.74 DD178 pKa = 2.96 ALRR181 pKa = 11.84 YY182 pKa = 9.87 GSTGRR187 pKa = 11.84 WSVQYY192 pKa = 10.6 KK193 pKa = 9.66 NQTILPSASVSSSTRR208 pKa = 11.84 RR209 pKa = 11.84 SVDD212 pKa = 3.18 EE213 pKa = 4.24 PDD215 pKa = 3.5 EE216 pKa = 4.37 ARR218 pKa = 11.84 SGPSTYY224 pKa = 10.23 PSTPPQISSRR234 pKa = 11.84 RR235 pKa = 11.84 SEE237 pKa = 4.03 TEE239 pKa = 3.17 EE240 pKa = 4.03 RR241 pKa = 11.84 GLVSSTLQTPPQTSGEE257 pKa = 3.97 VRR259 pKa = 11.84 LRR261 pKa = 11.84 RR262 pKa = 11.84 RR263 pKa = 11.84 RR264 pKa = 11.84 GKK266 pKa = 10.28 GEE268 pKa = 3.55 RR269 pKa = 11.84 TTRR272 pKa = 11.84 KK273 pKa = 9.76 SPTKK277 pKa = 10.09 RR278 pKa = 11.84 RR279 pKa = 11.84 RR280 pKa = 11.84 TDD282 pKa = 3.15 EE283 pKa = 3.92 TDD285 pKa = 3.36 SPGLEE290 pKa = 4.0 SVPSPEE296 pKa = 4.55 AVGTRR301 pKa = 11.84 HH302 pKa = 5.32 RR303 pKa = 11.84 TVPSTGLSRR312 pKa = 11.84 LRR314 pKa = 11.84 RR315 pKa = 11.84 LQIEE319 pKa = 4.16 ARR321 pKa = 11.84 DD322 pKa = 3.76 PPAIIVTGPANSLKK336 pKa = 9.9 CWRR339 pKa = 11.84 WRR341 pKa = 11.84 LKK343 pKa = 10.51 KK344 pKa = 10.35 YY345 pKa = 9.53 SRR347 pKa = 11.84 YY348 pKa = 7.16 YY349 pKa = 11.49 ARR351 pKa = 11.84 MSTVWSWAGEE361 pKa = 4.25 VCPKK365 pKa = 10.26 ASKK368 pKa = 10.84 NRR370 pKa = 11.84 MLIAFTDD377 pKa = 3.56 EE378 pKa = 4.15 TQRR381 pKa = 11.84 SVFLKK386 pKa = 10.6 LVSLPKK392 pKa = 9.39 HH393 pKa = 4.47 TTYY396 pKa = 11.56 GLAQLGILL404 pKa = 4.22
Molecular weight: 46.51 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 8.837
IPC2_protein 8.946
IPC_protein 8.931
Toseland 9.385
ProMoST 9.268
Dawson 9.721
Bjellqvist 9.472
Wikipedia 9.94
Rodwell 9.867
Grimsley 9.823
Solomon 9.75
Lehninger 9.692
Nozaki 9.443
DTASelect 9.443
Thurlkill 9.531
EMBOSS 9.823
Sillero 9.648
Patrickios 4.774
IPC_peptide 9.736
IPC2_peptide 8.126
IPC2.peptide.svr19 7.846
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
7
0
7
2424
97
603
346.3
39.22
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
4.992 ± 0.394
2.228 ± 0.804
6.436 ± 0.512
6.436 ± 0.256
4.579 ± 0.446
5.817 ± 0.775
1.733 ± 0.307
4.909 ± 0.688
5.652 ± 0.813
9.323 ± 0.673
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
1.898 ± 0.51
4.497 ± 0.658
6.353 ± 1.047
4.084 ± 0.408
5.487 ± 0.869
7.88 ± 0.428
6.518 ± 0.897
5.817 ± 0.37
1.238 ± 0.314
4.125 ± 0.533
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here