Amino acid dipepetide frequency for Erwinia phage Hena1

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
7.711AlaAla: 7.711 ± 0.5
0.935AlaCys: 0.935 ± 0.186
5.065AlaAsp: 5.065 ± 0.402
5.293AlaGlu: 5.293 ± 0.404
3.057AlaPhe: 3.057 ± 0.288
6.594AlaGly: 6.594 ± 0.483
0.89AlaHis: 0.89 ± 0.113
4.358AlaIle: 4.358 ± 0.333
5.795AlaLys: 5.795 ± 0.391
6.753AlaLeu: 6.753 ± 0.382
2.213AlaMet: 2.213 ± 0.217
3.491AlaAsn: 3.491 ± 0.364
2.966AlaPro: 2.966 ± 0.295
3.08AlaGln: 3.08 ± 0.365
3.445AlaArg: 3.445 ± 0.272
4.129AlaSer: 4.129 ± 0.358
5.727AlaThr: 5.727 ± 0.805
5.567AlaVal: 5.567 ± 0.44
1.049AlaTrp: 1.049 ± 0.184
3.057AlaTyr: 3.057 ± 0.264
0.0AlaXaa: 0.0 ± 0.0
Cys
0.753CysAla: 0.753 ± 0.14
0.205CysCys: 0.205 ± 0.068
0.684CysAsp: 0.684 ± 0.144
0.662CysGlu: 0.662 ± 0.108
0.479CysPhe: 0.479 ± 0.1
1.049CysGly: 1.049 ± 0.18
0.548CysHis: 0.548 ± 0.121
0.616CysIle: 0.616 ± 0.106
0.776CysLys: 0.776 ± 0.152
0.799CysLeu: 0.799 ± 0.155
0.297CysMet: 0.297 ± 0.088
0.342CysAsn: 0.342 ± 0.095
0.525CysPro: 0.525 ± 0.128
0.205CysGln: 0.205 ± 0.061
0.593CysArg: 0.593 ± 0.135
0.753CysSer: 0.753 ± 0.178
0.525CysThr: 0.525 ± 0.115
0.776CysVal: 0.776 ± 0.148
0.251CysTrp: 0.251 ± 0.076
0.456CysTyr: 0.456 ± 0.072
0.0CysXaa: 0.0 ± 0.0
Asp
4.882AspAla: 4.882 ± 0.316
0.57AspCys: 0.57 ± 0.119
3.673AspAsp: 3.673 ± 0.265
4.289AspGlu: 4.289 ± 0.351
2.806AspPhe: 2.806 ± 0.251
5.156AspGly: 5.156 ± 0.417
1.278AspHis: 1.278 ± 0.192
3.331AspIle: 3.331 ± 0.285
3.901AspLys: 3.901 ± 0.346
6.137AspLeu: 6.137 ± 0.397
1.825AspMet: 1.825 ± 0.205
2.578AspAsn: 2.578 ± 0.212
2.715AspPro: 2.715 ± 0.258
2.008AspGln: 2.008 ± 0.267
2.555AspArg: 2.555 ± 0.31
3.399AspSer: 3.399 ± 0.275
3.696AspThr: 3.696 ± 0.262
4.768AspVal: 4.768 ± 0.332
1.209AspTrp: 1.209 ± 0.154
2.783AspTyr: 2.783 ± 0.271
0.0AspXaa: 0.0 ± 0.0
Glu
5.088GluAla: 5.088 ± 0.342
0.662GluCys: 0.662 ± 0.103
4.015GluAsp: 4.015 ± 0.373
5.202GluGlu: 5.202 ± 0.521
2.806GluPhe: 2.806 ± 0.266
4.244GluGly: 4.244 ± 0.297
1.186GluHis: 1.186 ± 0.148
3.582GluIle: 3.582 ± 0.253
4.814GluLys: 4.814 ± 0.397
5.544GluLeu: 5.544 ± 0.394
2.167GluMet: 2.167 ± 0.251
3.194GluAsn: 3.194 ± 0.306
1.757GluPro: 1.757 ± 0.18
2.464GluGln: 2.464 ± 0.22
3.559GluArg: 3.559 ± 0.328
3.399GluSer: 3.399 ± 0.279
3.217GluThr: 3.217 ± 0.347
4.015GluVal: 4.015 ± 0.29
1.049GluTrp: 1.049 ± 0.167
2.487GluTyr: 2.487 ± 0.263
0.0GluXaa: 0.0 ± 0.0
Phe
2.897PheAla: 2.897 ± 0.253
0.388PheCys: 0.388 ± 0.094
3.24PheAsp: 3.24 ± 0.329
2.441PheGlu: 2.441 ± 0.253
1.734PhePhe: 1.734 ± 0.2
3.536PheGly: 3.536 ± 0.256
1.004PheHis: 1.004 ± 0.142
2.167PheIle: 2.167 ± 0.233
2.532PheLys: 2.532 ± 0.278
3.263PheLeu: 3.263 ± 0.249
1.164PheMet: 1.164 ± 0.16
2.008PheAsn: 2.008 ± 0.224
1.574PhePro: 1.574 ± 0.173
1.483PheGln: 1.483 ± 0.193
1.985PheArg: 1.985 ± 0.228
2.738PheSer: 2.738 ± 0.285
2.441PheThr: 2.441 ± 0.232
2.715PheVal: 2.715 ± 0.229
0.799PheTrp: 0.799 ± 0.142
1.688PheTyr: 1.688 ± 0.191
0.0PheXaa: 0.0 ± 0.0
Gly
4.951GlyAla: 4.951 ± 0.467
0.89GlyCys: 0.89 ± 0.169
4.517GlyAsp: 4.517 ± 0.469
4.244GlyGlu: 4.244 ± 0.305
4.015GlyPhe: 4.015 ± 0.304
5.476GlyGly: 5.476 ± 0.521
1.278GlyHis: 1.278 ± 0.165
4.061GlyIle: 4.061 ± 0.331
5.704GlyLys: 5.704 ± 0.423
5.658GlyLeu: 5.658 ± 0.354
2.418GlyMet: 2.418 ± 0.25
3.719GlyAsn: 3.719 ± 0.292
1.186GlyPro: 1.186 ± 0.138
2.281GlyGln: 2.281 ± 0.247
3.399GlyArg: 3.399 ± 0.286
4.951GlySer: 4.951 ± 0.506
4.905GlyThr: 4.905 ± 0.746
6.046GlyVal: 6.046 ± 0.359
1.072GlyTrp: 1.072 ± 0.175
3.08GlyTyr: 3.08 ± 0.277
0.0GlyXaa: 0.0 ± 0.0
His
1.118HisAla: 1.118 ± 0.168
0.479HisCys: 0.479 ± 0.12
1.3HisAsp: 1.3 ± 0.2
1.255HisGlu: 1.255 ± 0.157
0.821HisPhe: 0.821 ± 0.162
1.757HisGly: 1.757 ± 0.221
0.433HisHis: 0.433 ± 0.126
1.597HisIle: 1.597 ± 0.173
1.072HisLys: 1.072 ± 0.155
1.323HisLeu: 1.323 ± 0.197
0.456HisMet: 0.456 ± 0.138
1.072HisAsn: 1.072 ± 0.144
0.981HisPro: 0.981 ± 0.136
0.57HisGln: 0.57 ± 0.091
1.049HisArg: 1.049 ± 0.141
0.958HisSer: 0.958 ± 0.144
0.89HisThr: 0.89 ± 0.164
1.004HisVal: 1.004 ± 0.211
0.297HisTrp: 0.297 ± 0.08
1.004HisTyr: 1.004 ± 0.176
0.0HisXaa: 0.0 ± 0.0
Ile
5.407IleAla: 5.407 ± 0.436
0.662IleCys: 0.662 ± 0.145
3.947IleAsp: 3.947 ± 0.299
3.445IleGlu: 3.445 ± 0.289
2.35IlePhe: 2.35 ± 0.229
3.901IleGly: 3.901 ± 0.281
1.118IleHis: 1.118 ± 0.162
3.422IleIle: 3.422 ± 0.34
3.856IleLys: 3.856 ± 0.25
4.175IleLeu: 4.175 ± 0.39
1.415IleMet: 1.415 ± 0.166
2.806IleAsn: 2.806 ± 0.206
2.441IlePro: 2.441 ± 0.201
1.711IleGln: 1.711 ± 0.196
3.148IleArg: 3.148 ± 0.315
3.24IleSer: 3.24 ± 0.28
3.582IleThr: 3.582 ± 0.273
3.673IleVal: 3.673 ± 0.303
0.662IleTrp: 0.662 ± 0.124
1.62IleTyr: 1.62 ± 0.198
0.0IleXaa: 0.0 ± 0.0
Lys
5.909LysAla: 5.909 ± 0.463
0.57LysCys: 0.57 ± 0.127
4.152LysAsp: 4.152 ± 0.317
4.221LysGlu: 4.221 ± 0.388
2.647LysPhe: 2.647 ± 0.216
4.837LysGly: 4.837 ± 0.357
1.437LysHis: 1.437 ± 0.21
4.061LysIle: 4.061 ± 0.287
4.335LysLys: 4.335 ± 0.401
4.882LysLeu: 4.882 ± 0.32
2.167LysMet: 2.167 ± 0.195
2.647LysAsn: 2.647 ± 0.228
2.647LysPro: 2.647 ± 0.286
2.51LysGln: 2.51 ± 0.224
3.924LysArg: 3.924 ± 0.354
3.513LysSer: 3.513 ± 0.272
4.084LysThr: 4.084 ± 0.274
5.065LysVal: 5.065 ± 0.351
1.027LysTrp: 1.027 ± 0.154
2.464LysTyr: 2.464 ± 0.281
0.0LysXaa: 0.0 ± 0.0
Leu
6.708LeuAla: 6.708 ± 0.382
0.981LeuCys: 0.981 ± 0.151
5.316LeuAsp: 5.316 ± 0.344
6.0LeuGlu: 6.0 ± 0.442
3.171LeuPhe: 3.171 ± 0.227
5.042LeuGly: 5.042 ± 0.382
1.757LeuHis: 1.757 ± 0.207
4.586LeuIle: 4.586 ± 0.379
5.225LeuLys: 5.225 ± 0.417
6.114LeuLeu: 6.114 ± 0.461
2.167LeuMet: 2.167 ± 0.24
3.993LeuAsn: 3.993 ± 0.278
3.445LeuPro: 3.445 ± 0.243
2.304LeuGln: 2.304 ± 0.233
3.833LeuArg: 3.833 ± 0.36
5.316LeuSer: 5.316 ± 0.344
5.316LeuThr: 5.316 ± 0.349
5.202LeuVal: 5.202 ± 0.361
1.118LeuTrp: 1.118 ± 0.185
2.852LeuTyr: 2.852 ± 0.27
0.0LeuXaa: 0.0 ± 0.0
Met
2.806MetAla: 2.806 ± 0.267
0.251MetCys: 0.251 ± 0.081
1.118MetAsp: 1.118 ± 0.182
1.711MetGlu: 1.711 ± 0.194
1.049MetPhe: 1.049 ± 0.153
1.46MetGly: 1.46 ± 0.158
0.593MetHis: 0.593 ± 0.123
1.916MetIle: 1.916 ± 0.225
2.259MetLys: 2.259 ± 0.223
2.51MetLeu: 2.51 ± 0.206
0.799MetMet: 0.799 ± 0.164
1.369MetAsn: 1.369 ± 0.18
0.821MetPro: 0.821 ± 0.126
1.232MetGln: 1.232 ± 0.185
1.209MetArg: 1.209 ± 0.18
2.578MetSer: 2.578 ± 0.222
1.985MetThr: 1.985 ± 0.221
1.665MetVal: 1.665 ± 0.2
0.342MetTrp: 0.342 ± 0.075
0.799MetTyr: 0.799 ± 0.152
0.0MetXaa: 0.0 ± 0.0
Asn
3.924AsnAla: 3.924 ± 0.324
0.502AsnCys: 0.502 ± 0.119
2.441AsnAsp: 2.441 ± 0.241
2.167AsnGlu: 2.167 ± 0.183
2.008AsnPhe: 2.008 ± 0.235
4.312AsnGly: 4.312 ± 0.334
0.867AsnHis: 0.867 ± 0.149
2.989AsnIle: 2.989 ± 0.247
2.875AsnLys: 2.875 ± 0.245
3.879AsnLeu: 3.879 ± 0.279
1.369AsnMet: 1.369 ± 0.156
2.259AsnAsn: 2.259 ± 0.232
2.304AsnPro: 2.304 ± 0.246
1.78AsnGln: 1.78 ± 0.194
2.281AsnArg: 2.281 ± 0.213
2.464AsnSer: 2.464 ± 0.285
2.715AsnThr: 2.715 ± 0.342
2.875AsnVal: 2.875 ± 0.302
0.958AsnTrp: 0.958 ± 0.177
1.734AsnTyr: 1.734 ± 0.198
0.0AsnXaa: 0.0 ± 0.0
Pro
3.559ProAla: 3.559 ± 0.312
0.365ProCys: 0.365 ± 0.082
2.783ProAsp: 2.783 ± 0.26
3.308ProGlu: 3.308 ± 0.342
1.529ProPhe: 1.529 ± 0.17
2.601ProGly: 2.601 ± 0.237
0.662ProHis: 0.662 ± 0.128
1.78ProIle: 1.78 ± 0.189
2.19ProLys: 2.19 ± 0.214
2.555ProLeu: 2.555 ± 0.247
0.981ProMet: 0.981 ± 0.154
1.483ProAsn: 1.483 ± 0.213
1.232ProPro: 1.232 ± 0.221
1.232ProGln: 1.232 ± 0.152
1.483ProArg: 1.483 ± 0.165
2.145ProSer: 2.145 ± 0.208
2.555ProThr: 2.555 ± 0.244
2.943ProVal: 2.943 ± 0.281
0.479ProTrp: 0.479 ± 0.103
1.278ProTyr: 1.278 ± 0.182
0.0ProXaa: 0.0 ± 0.0
Gln
2.989GlnAla: 2.989 ± 0.298
0.251GlnCys: 0.251 ± 0.073
2.031GlnAsp: 2.031 ± 0.316
2.464GlnGlu: 2.464 ± 0.259
1.415GlnPhe: 1.415 ± 0.178
2.327GlnGly: 2.327 ± 0.192
0.342GlnHis: 0.342 ± 0.084
2.441GlnIle: 2.441 ± 0.23
2.487GlnLys: 2.487 ± 0.233
2.396GlnLeu: 2.396 ± 0.21
0.981GlnMet: 0.981 ± 0.183
1.483GlnAsn: 1.483 ± 0.175
1.232GlnPro: 1.232 ± 0.182
1.141GlnGln: 1.141 ± 0.19
1.871GlnArg: 1.871 ± 0.189
1.871GlnSer: 1.871 ± 0.224
2.167GlnThr: 2.167 ± 0.254
2.51GlnVal: 2.51 ± 0.219
0.662GlnTrp: 0.662 ± 0.141
1.506GlnTyr: 1.506 ± 0.179
0.0GlnXaa: 0.0 ± 0.0
Arg
3.468ArgAla: 3.468 ± 0.275
0.662ArgCys: 0.662 ± 0.132
3.057ArgAsp: 3.057 ± 0.285
3.194ArgGlu: 3.194 ± 0.276
1.894ArgPhe: 1.894 ± 0.191
3.331ArgGly: 3.331 ± 0.26
1.049ArgHis: 1.049 ± 0.153
2.738ArgIle: 2.738 ± 0.298
3.422ArgLys: 3.422 ± 0.337
4.266ArgLeu: 4.266 ± 0.346
1.437ArgMet: 1.437 ± 0.175
2.008ArgAsn: 2.008 ± 0.243
1.711ArgPro: 1.711 ± 0.195
2.167ArgGln: 2.167 ± 0.29
3.263ArgArg: 3.263 ± 0.348
2.373ArgSer: 2.373 ± 0.253
2.806ArgThr: 2.806 ± 0.279
3.034ArgVal: 3.034 ± 0.318
0.57ArgTrp: 0.57 ± 0.11
1.643ArgTyr: 1.643 ± 0.2
0.0ArgXaa: 0.0 ± 0.0
Ser
4.609SerAla: 4.609 ± 0.298
0.593SerCys: 0.593 ± 0.108
4.38SerAsp: 4.38 ± 0.337
2.852SerGlu: 2.852 ± 0.256
3.012SerPhe: 3.012 ± 0.235
5.156SerGly: 5.156 ± 0.51
0.867SerHis: 0.867 ± 0.151
2.897SerIle: 2.897 ± 0.294
3.605SerLys: 3.605 ± 0.299
4.449SerLeu: 4.449 ± 0.357
1.574SerMet: 1.574 ± 0.199
2.852SerAsn: 2.852 ± 0.276
2.259SerPro: 2.259 ± 0.187
2.213SerGln: 2.213 ± 0.229
2.647SerArg: 2.647 ± 0.259
3.468SerSer: 3.468 ± 0.404
3.856SerThr: 3.856 ± 0.321
4.015SerVal: 4.015 ± 0.344
1.072SerTrp: 1.072 ± 0.154
2.19SerTyr: 2.19 ± 0.284
0.0SerXaa: 0.0 ± 0.0
Thr
5.453ThrAla: 5.453 ± 0.578
0.89ThrCys: 0.89 ± 0.156
3.628ThrAsp: 3.628 ± 0.301
3.719ThrGlu: 3.719 ± 0.289
2.555ThrPhe: 2.555 ± 0.268
4.745ThrGly: 4.745 ± 0.528
1.346ThrHis: 1.346 ± 0.142
3.148ThrIle: 3.148 ± 0.282
4.426ThrLys: 4.426 ± 0.287
5.704ThrLeu: 5.704 ± 0.368
1.346ThrMet: 1.346 ± 0.185
2.806ThrAsn: 2.806 ± 0.387
2.966ThrPro: 2.966 ± 0.28
2.053ThrGln: 2.053 ± 0.217
2.259ThrArg: 2.259 ± 0.224
3.605ThrSer: 3.605 ± 0.379
3.833ThrThr: 3.833 ± 0.627
4.996ThrVal: 4.996 ± 0.437
1.392ThrTrp: 1.392 ± 0.258
1.962ThrTyr: 1.962 ± 0.215
0.0ThrXaa: 0.0 ± 0.0
Val
5.316ValAla: 5.316 ± 0.415
0.867ValCys: 0.867 ± 0.169
4.928ValAsp: 4.928 ± 0.29
4.677ValGlu: 4.677 ± 0.273
2.259ValPhe: 2.259 ± 0.23
4.517ValGly: 4.517 ± 0.39
1.278ValHis: 1.278 ± 0.231
4.472ValIle: 4.472 ± 0.348
4.677ValLys: 4.677 ± 0.379
4.951ValLeu: 4.951 ± 0.314
2.213ValMet: 2.213 ± 0.277
3.422ValAsn: 3.422 ± 0.303
2.464ValPro: 2.464 ± 0.285
2.327ValGln: 2.327 ± 0.245
3.103ValArg: 3.103 ± 0.335
4.266ValSer: 4.266 ± 0.303
5.339ValThr: 5.339 ± 0.405
5.658ValVal: 5.658 ± 0.409
1.095ValTrp: 1.095 ± 0.138
2.715ValTyr: 2.715 ± 0.245
0.0ValXaa: 0.0 ± 0.0
Trp
1.209TrpAla: 1.209 ± 0.155
0.114TrpCys: 0.114 ± 0.06
1.095TrpAsp: 1.095 ± 0.16
1.027TrpGlu: 1.027 ± 0.171
0.593TrpPhe: 0.593 ± 0.113
1.072TrpGly: 1.072 ± 0.17
0.342TrpHis: 0.342 ± 0.072
0.958TrpIle: 0.958 ± 0.161
1.049TrpLys: 1.049 ± 0.172
1.597TrpLeu: 1.597 ± 0.206
0.525TrpMet: 0.525 ± 0.109
1.004TrpAsn: 1.004 ± 0.175
0.297TrpPro: 0.297 ± 0.078
0.548TrpGln: 0.548 ± 0.115
0.616TrpArg: 0.616 ± 0.109
1.164TrpSer: 1.164 ± 0.162
0.935TrpThr: 0.935 ± 0.192
1.027TrpVal: 1.027 ± 0.13
0.274TrpTrp: 0.274 ± 0.074
0.616TrpTyr: 0.616 ± 0.102
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.441TyrAla: 2.441 ± 0.255
0.456TyrCys: 0.456 ± 0.111
2.19TyrAsp: 2.19 ± 0.22
2.281TyrGlu: 2.281 ± 0.256
1.597TyrPhe: 1.597 ± 0.219
2.738TyrGly: 2.738 ± 0.29
1.164TyrHis: 1.164 ± 0.168
1.46TyrIle: 1.46 ± 0.173
2.099TyrLys: 2.099 ± 0.203
3.491TyrLeu: 3.491 ± 0.357
0.935TyrMet: 0.935 ± 0.135
2.167TyrAsn: 2.167 ± 0.206
1.62TyrPro: 1.62 ± 0.182
1.255TyrGln: 1.255 ± 0.189
1.871TyrArg: 1.871 ± 0.191
2.236TyrSer: 2.236 ± 0.229
2.259TyrThr: 2.259 ± 0.206
2.989TyrVal: 2.989 ± 0.245
0.684TyrTrp: 0.684 ± 0.155
1.392TyrTyr: 1.392 ± 0.182
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 240 proteins (43832 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski