Amino acid dipepetide frequency for Streptococcus phage phi-SsuNJ2_rum

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
3.847AlaAla: 3.847 ± 1.067
0.504AlaCys: 0.504 ± 0.153
3.657AlaAsp: 3.657 ± 0.421
4.414AlaGlu: 4.414 ± 0.55
2.901AlaPhe: 2.901 ± 0.432
3.721AlaGly: 3.721 ± 0.356
0.378AlaHis: 0.378 ± 0.148
5.297AlaIle: 5.297 ± 0.715
5.675AlaLys: 5.675 ± 0.57
6.495AlaLeu: 6.495 ± 0.747
1.576AlaMet: 1.576 ± 0.238
3.216AlaAsn: 3.216 ± 0.587
1.45AlaPro: 1.45 ± 0.325
2.585AlaGln: 2.585 ± 0.643
2.649AlaArg: 2.649 ± 0.346
4.856AlaSer: 4.856 ± 0.576
3.468AlaThr: 3.468 ± 0.555
4.036AlaVal: 4.036 ± 0.658
0.883AlaTrp: 0.883 ± 0.353
2.712AlaTyr: 2.712 ± 0.434
0.0AlaXaa: 0.0 ± 0.0
Cys
0.694CysAla: 0.694 ± 0.256
0.315CysCys: 0.315 ± 0.123
0.441CysAsp: 0.441 ± 0.172
0.441CysGlu: 0.441 ± 0.153
0.441CysPhe: 0.441 ± 0.173
0.694CysGly: 0.694 ± 0.196
0.189CysHis: 0.189 ± 0.098
0.252CysIle: 0.252 ± 0.119
0.883CysLys: 0.883 ± 0.259
0.694CysLeu: 0.694 ± 0.224
0.126CysMet: 0.126 ± 0.088
0.315CysAsn: 0.315 ± 0.168
0.568CysPro: 0.568 ± 0.179
0.694CysGln: 0.694 ± 0.187
0.883CysArg: 0.883 ± 0.285
0.694CysSer: 0.694 ± 0.205
0.189CysThr: 0.189 ± 0.108
0.694CysVal: 0.694 ± 0.204
0.0CysTrp: 0.0 ± 0.0
0.631CysTyr: 0.631 ± 0.221
0.0CysXaa: 0.0 ± 0.0
Asp
2.838AspAla: 2.838 ± 0.397
0.82AspCys: 0.82 ± 0.278
2.964AspAsp: 2.964 ± 0.66
5.171AspGlu: 5.171 ± 0.831
3.784AspPhe: 3.784 ± 0.514
4.729AspGly: 4.729 ± 0.464
0.946AspHis: 0.946 ± 0.277
5.171AspIle: 5.171 ± 0.529
3.973AspLys: 3.973 ± 0.455
4.225AspLeu: 4.225 ± 0.691
2.018AspMet: 2.018 ± 0.331
2.838AspAsn: 2.838 ± 0.398
1.45AspPro: 1.45 ± 0.345
1.829AspGln: 1.829 ± 0.353
2.901AspArg: 2.901 ± 0.522
2.964AspSer: 2.964 ± 0.509
2.396AspThr: 2.396 ± 0.392
3.279AspVal: 3.279 ± 0.383
0.757AspTrp: 0.757 ± 0.217
2.964AspTyr: 2.964 ± 0.503
0.0AspXaa: 0.0 ± 0.0
Glu
4.099GluAla: 4.099 ± 0.488
0.441GluCys: 0.441 ± 0.171
4.54GluAsp: 4.54 ± 0.543
5.675GluGlu: 5.675 ± 0.778
2.081GluPhe: 2.081 ± 0.363
4.666GluGly: 4.666 ± 0.532
1.198GluHis: 1.198 ± 0.306
5.549GluIle: 5.549 ± 0.541
6.369GluLys: 6.369 ± 0.698
7.63GluLeu: 7.63 ± 0.722
2.459GluMet: 2.459 ± 0.403
3.468GluAsn: 3.468 ± 0.646
1.576GluPro: 1.576 ± 0.389
4.225GluGln: 4.225 ± 0.379
3.342GluArg: 3.342 ± 0.476
3.973GluSer: 3.973 ± 0.444
4.54GluThr: 4.54 ± 0.606
4.351GluVal: 4.351 ± 0.529
0.631GluTrp: 0.631 ± 0.237
2.396GluTyr: 2.396 ± 0.439
0.0GluXaa: 0.0 ± 0.0
Phe
2.081PheAla: 2.081 ± 0.358
0.568PheCys: 0.568 ± 0.181
2.585PheAsp: 2.585 ± 0.515
3.468PheGlu: 3.468 ± 0.441
1.64PhePhe: 1.64 ± 0.365
2.396PheGly: 2.396 ± 0.302
1.072PheHis: 1.072 ± 0.266
2.712PheIle: 2.712 ± 0.433
3.657PheLys: 3.657 ± 0.534
3.342PheLeu: 3.342 ± 0.474
0.883PheMet: 0.883 ± 0.249
1.955PheAsn: 1.955 ± 0.313
0.694PhePro: 0.694 ± 0.21
1.703PheGln: 1.703 ± 0.311
1.64PheArg: 1.64 ± 0.359
2.144PheSer: 2.144 ± 0.433
2.27PheThr: 2.27 ± 0.374
2.396PheVal: 2.396 ± 0.431
0.631PheTrp: 0.631 ± 0.187
2.081PheTyr: 2.081 ± 0.345
0.0PheXaa: 0.0 ± 0.0
Gly
3.594GlyAla: 3.594 ± 0.594
0.378GlyCys: 0.378 ± 0.148
3.594GlyAsp: 3.594 ± 0.557
3.847GlyGlu: 3.847 ± 0.435
2.522GlyPhe: 2.522 ± 0.4
3.468GlyGly: 3.468 ± 0.486
2.018GlyHis: 2.018 ± 0.433
6.243GlyIle: 6.243 ± 0.762
4.54GlyLys: 4.54 ± 0.52
4.919GlyLeu: 4.919 ± 0.641
1.766GlyMet: 1.766 ± 0.396
3.279GlyAsn: 3.279 ± 0.489
0.631GlyPro: 0.631 ± 0.182
2.838GlyGln: 2.838 ± 0.467
3.531GlyArg: 3.531 ± 0.365
3.468GlySer: 3.468 ± 0.408
4.225GlyThr: 4.225 ± 0.581
3.784GlyVal: 3.784 ± 0.541
0.568GlyTrp: 0.568 ± 0.154
3.027GlyTyr: 3.027 ± 0.44
0.0GlyXaa: 0.0 ± 0.0
His
0.82HisAla: 0.82 ± 0.227
0.315HisCys: 0.315 ± 0.152
1.324HisAsp: 1.324 ± 0.283
1.072HisGlu: 1.072 ± 0.25
1.135HisPhe: 1.135 ± 0.316
1.387HisGly: 1.387 ± 0.274
0.441HisHis: 0.441 ± 0.153
1.64HisIle: 1.64 ± 0.246
0.946HisLys: 0.946 ± 0.281
1.703HisLeu: 1.703 ± 0.337
0.504HisMet: 0.504 ± 0.183
0.757HisAsn: 0.757 ± 0.227
1.198HisPro: 1.198 ± 0.253
0.694HisGln: 0.694 ± 0.209
1.072HisArg: 1.072 ± 0.23
0.883HisSer: 0.883 ± 0.273
1.072HisThr: 1.072 ± 0.282
1.135HisVal: 1.135 ± 0.263
0.252HisTrp: 0.252 ± 0.106
0.568HisTyr: 0.568 ± 0.187
0.0HisXaa: 0.0 ± 0.0
Ile
4.666IleAla: 4.666 ± 0.412
0.631IleCys: 0.631 ± 0.249
5.36IleAsp: 5.36 ± 0.461
4.729IleGlu: 4.729 ± 0.704
1.829IlePhe: 1.829 ± 0.254
4.856IleGly: 4.856 ± 0.608
1.261IleHis: 1.261 ± 0.297
3.973IleIle: 3.973 ± 0.469
4.793IleLys: 4.793 ± 0.609
6.243IleLeu: 6.243 ± 0.531
1.324IleMet: 1.324 ± 0.311
3.342IleAsn: 3.342 ± 0.331
2.838IlePro: 2.838 ± 0.437
2.964IleGln: 2.964 ± 0.352
3.09IleArg: 3.09 ± 0.407
5.486IleSer: 5.486 ± 0.99
4.729IleThr: 4.729 ± 0.742
4.666IleVal: 4.666 ± 0.581
1.072IleTrp: 1.072 ± 0.349
2.333IleTyr: 2.333 ± 0.385
0.0IleXaa: 0.0 ± 0.0
Lys
5.675LysAla: 5.675 ± 0.468
0.441LysCys: 0.441 ± 0.177
4.036LysAsp: 4.036 ± 0.547
4.793LysGlu: 4.793 ± 0.482
3.027LysPhe: 3.027 ± 0.426
3.91LysGly: 3.91 ± 0.346
1.45LysHis: 1.45 ± 0.355
5.549LysIle: 5.549 ± 0.672
4.919LysLys: 4.919 ± 0.689
6.495LysLeu: 6.495 ± 0.475
1.955LysMet: 1.955 ± 0.326
3.09LysAsn: 3.09 ± 0.507
2.207LysPro: 2.207 ± 0.358
3.531LysGln: 3.531 ± 0.445
4.036LysArg: 4.036 ± 0.71
5.297LysSer: 5.297 ± 0.646
4.603LysThr: 4.603 ± 0.471
4.793LysVal: 4.793 ± 0.626
1.072LysTrp: 1.072 ± 0.257
2.585LysTyr: 2.585 ± 0.413
0.0LysXaa: 0.0 ± 0.0
Leu
5.865LeuAla: 5.865 ± 0.712
0.568LeuCys: 0.568 ± 0.176
5.36LeuAsp: 5.36 ± 0.731
7.756LeuGlu: 7.756 ± 0.775
3.468LeuPhe: 3.468 ± 0.503
5.36LeuGly: 5.36 ± 0.629
1.576LeuHis: 1.576 ± 0.295
4.982LeuIle: 4.982 ± 0.552
7.378LeuLys: 7.378 ± 0.767
8.261LeuLeu: 8.261 ± 1.024
1.703LeuMet: 1.703 ± 0.316
4.477LeuAsn: 4.477 ± 0.598
3.468LeuPro: 3.468 ± 0.592
3.531LeuGln: 3.531 ± 0.531
3.721LeuArg: 3.721 ± 0.485
7.693LeuSer: 7.693 ± 0.574
6.432LeuThr: 6.432 ± 0.609
5.612LeuVal: 5.612 ± 0.631
0.441LeuTrp: 0.441 ± 0.158
3.657LeuTyr: 3.657 ± 0.607
0.0LeuXaa: 0.0 ± 0.0
Met
2.144MetAla: 2.144 ± 0.403
0.189MetCys: 0.189 ± 0.112
1.45MetAsp: 1.45 ± 0.305
1.829MetGlu: 1.829 ± 0.342
0.883MetPhe: 0.883 ± 0.184
1.955MetGly: 1.955 ± 0.326
0.126MetHis: 0.126 ± 0.091
1.64MetIle: 1.64 ± 0.299
1.892MetLys: 1.892 ± 0.325
1.64MetLeu: 1.64 ± 0.302
0.694MetMet: 0.694 ± 0.242
1.135MetAsn: 1.135 ± 0.275
0.694MetPro: 0.694 ± 0.191
0.82MetGln: 0.82 ± 0.245
1.072MetArg: 1.072 ± 0.259
2.27MetSer: 2.27 ± 0.323
1.703MetThr: 1.703 ± 0.411
1.576MetVal: 1.576 ± 0.301
0.189MetTrp: 0.189 ± 0.108
0.315MetTyr: 0.315 ± 0.12
0.0MetXaa: 0.0 ± 0.0
Asn
4.099AsnAla: 4.099 ± 0.604
0.378AsnCys: 0.378 ± 0.162
2.585AsnAsp: 2.585 ± 0.389
2.522AsnGlu: 2.522 ± 0.451
1.892AsnPhe: 1.892 ± 0.338
4.54AsnGly: 4.54 ± 0.513
1.324AsnHis: 1.324 ± 0.243
2.459AsnIle: 2.459 ± 0.326
2.712AsnLys: 2.712 ± 0.389
4.603AsnLeu: 4.603 ± 0.636
0.946AsnMet: 0.946 ± 0.239
1.766AsnAsn: 1.766 ± 0.304
2.333AsnPro: 2.333 ± 0.322
2.27AsnGln: 2.27 ± 0.336
2.081AsnArg: 2.081 ± 0.318
2.712AsnSer: 2.712 ± 0.371
2.207AsnThr: 2.207 ± 0.407
2.333AsnVal: 2.333 ± 0.44
1.072AsnTrp: 1.072 ± 0.284
1.387AsnTyr: 1.387 ± 0.324
0.0AsnXaa: 0.0 ± 0.0
Pro
1.64ProAla: 1.64 ± 0.37
0.378ProCys: 0.378 ± 0.14
1.829ProAsp: 1.829 ± 0.353
2.333ProGlu: 2.333 ± 0.384
1.324ProPhe: 1.324 ± 0.249
0.883ProGly: 0.883 ± 0.272
0.757ProHis: 0.757 ± 0.213
1.766ProIle: 1.766 ± 0.344
2.081ProLys: 2.081 ± 0.434
3.216ProLeu: 3.216 ± 0.527
0.757ProMet: 0.757 ± 0.203
1.387ProAsn: 1.387 ± 0.326
1.009ProPro: 1.009 ± 0.263
1.009ProGln: 1.009 ± 0.248
1.955ProArg: 1.955 ± 0.288
2.459ProSer: 2.459 ± 0.397
2.27ProThr: 2.27 ± 0.446
2.396ProVal: 2.396 ± 0.431
0.315ProTrp: 0.315 ± 0.134
1.45ProTyr: 1.45 ± 0.315
0.0ProXaa: 0.0 ± 0.0
Gln
3.91GlnAla: 3.91 ± 0.584
0.441GlnCys: 0.441 ± 0.13
1.829GlnAsp: 1.829 ± 0.301
3.468GlnGlu: 3.468 ± 0.384
1.703GlnPhe: 1.703 ± 0.321
2.018GlnGly: 2.018 ± 0.312
0.694GlnHis: 0.694 ± 0.184
2.712GlnIle: 2.712 ± 0.468
3.153GlnLys: 3.153 ± 0.506
4.54GlnLeu: 4.54 ± 0.454
1.198GlnMet: 1.198 ± 0.226
1.766GlnAsn: 1.766 ± 0.325
1.324GlnPro: 1.324 ± 0.336
1.766GlnGln: 1.766 ± 0.366
1.955GlnArg: 1.955 ± 0.48
2.649GlnSer: 2.649 ± 0.364
2.901GlnThr: 2.901 ± 0.6
3.594GlnVal: 3.594 ± 0.472
0.82GlnTrp: 0.82 ± 0.263
1.135GlnTyr: 1.135 ± 0.293
0.0GlnXaa: 0.0 ± 0.0
Arg
2.396ArgAla: 2.396 ± 0.397
0.694ArgCys: 0.694 ± 0.201
2.649ArgAsp: 2.649 ± 0.377
3.279ArgGlu: 3.279 ± 0.448
1.955ArgPhe: 1.955 ± 0.354
2.396ArgGly: 2.396 ± 0.327
0.82ArgHis: 0.82 ± 0.246
3.153ArgIle: 3.153 ± 0.527
4.351ArgLys: 4.351 ± 0.673
4.793ArgLeu: 4.793 ± 0.479
0.694ArgMet: 0.694 ± 0.248
2.207ArgAsn: 2.207 ± 0.305
1.387ArgPro: 1.387 ± 0.321
2.775ArgGln: 2.775 ± 0.323
1.955ArgArg: 1.955 ± 0.393
2.901ArgSer: 2.901 ± 0.413
2.775ArgThr: 2.775 ± 0.589
3.342ArgVal: 3.342 ± 0.381
0.946ArgTrp: 0.946 ± 0.253
1.766ArgTyr: 1.766 ± 0.325
0.0ArgXaa: 0.0 ± 0.0
Ser
4.288SerAla: 4.288 ± 0.566
0.504SerCys: 0.504 ± 0.171
4.414SerAsp: 4.414 ± 0.498
4.793SerGlu: 4.793 ± 0.531
2.838SerPhe: 2.838 ± 0.475
4.666SerGly: 4.666 ± 0.612
1.45SerHis: 1.45 ± 0.325
5.108SerIle: 5.108 ± 0.55
4.414SerLys: 4.414 ± 0.507
5.612SerLeu: 5.612 ± 0.583
1.703SerMet: 1.703 ± 0.302
3.342SerAsn: 3.342 ± 0.578
2.396SerPro: 2.396 ± 0.408
2.838SerGln: 2.838 ± 0.628
3.468SerArg: 3.468 ± 0.51
4.793SerSer: 4.793 ± 0.647
4.54SerThr: 4.54 ± 0.52
3.847SerVal: 3.847 ± 0.486
0.946SerTrp: 0.946 ± 0.163
2.901SerTyr: 2.901 ± 0.512
0.0SerXaa: 0.0 ± 0.0
Thr
4.351ThrAla: 4.351 ± 0.623
0.315ThrCys: 0.315 ± 0.121
2.712ThrAsp: 2.712 ± 0.441
5.108ThrGlu: 5.108 ± 0.442
2.27ThrPhe: 2.27 ± 0.492
4.162ThrGly: 4.162 ± 0.653
0.883ThrHis: 0.883 ± 0.205
4.856ThrIle: 4.856 ± 0.571
4.351ThrLys: 4.351 ± 0.494
5.36ThrLeu: 5.36 ± 0.541
1.135ThrMet: 1.135 ± 0.207
2.649ThrAsn: 2.649 ± 0.392
2.27ThrPro: 2.27 ± 0.447
2.081ThrGln: 2.081 ± 0.702
2.018ThrArg: 2.018 ± 0.336
4.856ThrSer: 4.856 ± 0.771
4.856ThrThr: 4.856 ± 0.922
5.549ThrVal: 5.549 ± 0.735
0.82ThrTrp: 0.82 ± 0.222
2.396ThrTyr: 2.396 ± 0.408
0.0ThrXaa: 0.0 ± 0.0
Val
3.657ValAla: 3.657 ± 0.527
0.883ValCys: 0.883 ± 0.22
3.216ValAsp: 3.216 ± 0.513
4.666ValGlu: 4.666 ± 0.553
2.144ValPhe: 2.144 ± 0.337
3.721ValGly: 3.721 ± 0.613
1.198ValHis: 1.198 ± 0.235
4.036ValIle: 4.036 ± 0.543
4.099ValLys: 4.099 ± 0.477
6.81ValLeu: 6.81 ± 0.507
1.261ValMet: 1.261 ± 0.274
2.459ValAsn: 2.459 ± 0.374
2.27ValPro: 2.27 ± 0.299
2.775ValGln: 2.775 ± 0.409
3.279ValArg: 3.279 ± 0.46
4.856ValSer: 4.856 ± 0.63
4.54ValThr: 4.54 ± 0.472
3.09ValVal: 3.09 ± 0.516
1.135ValTrp: 1.135 ± 0.21
2.585ValTyr: 2.585 ± 0.513
0.0ValXaa: 0.0 ± 0.0
Trp
1.198TrpAla: 1.198 ± 0.288
0.189TrpCys: 0.189 ± 0.111
0.631TrpAsp: 0.631 ± 0.196
1.135TrpGlu: 1.135 ± 0.292
0.694TrpPhe: 0.694 ± 0.223
0.757TrpGly: 0.757 ± 0.19
0.189TrpHis: 0.189 ± 0.099
0.694TrpIle: 0.694 ± 0.197
0.82TrpLys: 0.82 ± 0.28
0.946TrpLeu: 0.946 ± 0.235
0.441TrpMet: 0.441 ± 0.146
1.072TrpAsn: 1.072 ± 0.334
0.063TrpPro: 0.063 ± 0.061
0.757TrpGln: 0.757 ± 0.223
0.441TrpArg: 0.441 ± 0.261
0.757TrpSer: 0.757 ± 0.289
1.009TrpThr: 1.009 ± 0.334
0.568TrpVal: 0.568 ± 0.179
0.252TrpTrp: 0.252 ± 0.117
0.441TrpTyr: 0.441 ± 0.131
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.459TyrAla: 2.459 ± 0.348
0.883TyrCys: 0.883 ± 0.252
2.838TyrAsp: 2.838 ± 0.478
2.712TyrGlu: 2.712 ± 0.418
1.387TyrPhe: 1.387 ± 0.326
1.955TyrGly: 1.955 ± 0.347
0.946TyrHis: 0.946 ± 0.303
2.396TyrIle: 2.396 ± 0.344
2.459TyrLys: 2.459 ± 0.443
3.784TyrLeu: 3.784 ± 0.489
1.009TyrMet: 1.009 ± 0.245
1.703TyrAsn: 1.703 ± 0.326
1.387TyrPro: 1.387 ± 0.298
1.892TyrGln: 1.892 ± 0.338
2.144TyrArg: 2.144 ± 0.336
3.153TyrSer: 3.153 ± 0.512
2.333TyrThr: 2.333 ± 0.417
1.64TyrVal: 1.64 ± 0.299
0.252TyrTrp: 0.252 ± 0.117
1.198TyrTyr: 1.198 ± 0.287
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 61 proteins (15859 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski