Mycobacterium virus Fruitloop

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; Cheoctovirus

Average proteome isoelectric point is 6.1

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 102 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|B5U4Q1|B5U4Q1_9CAUD Uncharacterized protein OS=Mycobacterium virus Fruitloop OX=563119 GN=52 PE=4 SV=1
MM1 pKa = 7.26NLVEE5 pKa = 4.34RR6 pKa = 11.84LNARR10 pKa = 11.84FNNVIHH16 pKa = 7.66DD17 pKa = 4.05GLALVGAVVDD27 pKa = 3.85PWLAKK32 pKa = 10.34LEE34 pKa = 4.03RR35 pKa = 11.84QAMSNALGRR44 pKa = 11.84DD45 pKa = 3.36FGLDD49 pKa = 3.43YY50 pKa = 11.59GDD52 pKa = 3.74VLAAAEE58 pKa = 4.38AEE60 pKa = 4.57VEE62 pKa = 4.31VHH64 pKa = 6.55EE65 pKa = 5.07PAPCVCGHH73 pKa = 6.3LRR75 pKa = 11.84EE76 pKa = 3.99DD77 pKa = 3.83HH78 pKa = 6.06YY79 pKa = 11.45RR80 pKa = 11.84VGGFIDD86 pKa = 3.93PCSKK90 pKa = 10.56CDD92 pKa = 4.56CLDD95 pKa = 3.66LLYY98 pKa = 10.75EE99 pKa = 4.42SSAEE103 pKa = 3.91LWASADD109 pKa = 3.67PSPVSVGDD117 pKa = 3.55IGPGAGMVPPPPPAPGPSKK136 pKa = 9.68CTCPTAEE143 pKa = 4.59CEE145 pKa = 4.71LLAEE149 pKa = 5.34DD150 pKa = 4.11ICDD153 pKa = 3.51EE154 pKa = 4.29AEE156 pKa = 3.92EE157 pKa = 5.46AEE159 pKa = 4.32LLDD162 pKa = 3.84EE163 pKa = 4.75FMEE166 pKa = 4.61LGEE169 pKa = 4.33YY170 pKa = 10.61LDD172 pKa = 3.75TATAEE177 pKa = 3.82EE178 pKa = 4.16LAAIRR183 pKa = 11.84QHH185 pKa = 5.61TEE187 pKa = 3.03VSRR190 pKa = 11.84ADD192 pKa = 3.74LEE194 pKa = 4.36LHH196 pKa = 6.0LRR198 pKa = 11.84WYY200 pKa = 5.32TTARR204 pKa = 11.84VSPEE208 pKa = 3.64VVAQSLLDD216 pKa = 3.66SYY218 pKa = 11.22RR219 pKa = 11.84ITPRR223 pKa = 3.23

Molecular weight: 24.22 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|B5U4P5|B5U4P5_9CAUD Bro-N domain-containing protein OS=Mycobacterium virus Fruitloop OX=563119 GN=46 PE=4 SV=1
MM1 pKa = 7.3HH2 pKa = 7.59RR3 pKa = 11.84ADD5 pKa = 3.78SLQLRR10 pKa = 11.84ACNFRR15 pKa = 11.84TSQVNALLARR25 pKa = 11.84THH27 pKa = 7.32RR28 pKa = 11.84EE29 pKa = 3.6LLQFALVRR37 pKa = 11.84STVGSMVKK45 pKa = 9.65QSYY48 pKa = 9.07GVWQEE53 pKa = 3.32LRR55 pKa = 11.84IIRR58 pKa = 11.84EE59 pKa = 3.87RR60 pKa = 11.84TGWSSAEE67 pKa = 4.01LSRR70 pKa = 11.84EE71 pKa = 4.22SGVSAPYY78 pKa = 10.54LSQLEE83 pKa = 4.39NGDD86 pKa = 3.36RR87 pKa = 11.84WPNATVTKK95 pKa = 10.13KK96 pKa = 10.62LAVALKK102 pKa = 10.08VPVSVLEE109 pKa = 4.36RR110 pKa = 11.84PAEE113 pKa = 4.16QKK115 pKa = 10.96NPAAA119 pKa = 4.93

Molecular weight: 13.33 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

102

0

102

17961

30

1174

176.1

19.3

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

10.428 ± 0.445

1.069 ± 0.128

6.826 ± 0.215

5.84 ± 0.276

3.001 ± 0.179

8.825 ± 0.591

2.255 ± 0.189

4.398 ± 0.188

3.213 ± 0.194

7.438 ± 0.234

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.338 ± 0.111

3.235 ± 0.165

5.935 ± 0.193

3.268 ± 0.206

6.909 ± 0.353

5.93 ± 0.261

6.625 ± 0.242

7.388 ± 0.278

2.416 ± 0.168

2.661 ± 0.169
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski