Escherichia phage DE3

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; Lambdavirus; Escherichia virus DE3

Average proteome isoelectric point is 6.72

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 56 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0K2FIG2|A0A0K2FIG2_9CAUD DNA packaging protein OS=Escherichia phage DE3 OX=482822 GN=nu1 PE=4 SV=1
MM1 pKa = 7.39KK2 pKa = 9.4HH3 pKa = 5.21TEE5 pKa = 3.58LRR7 pKa = 11.84AAVLDD12 pKa = 3.88ALEE15 pKa = 4.33KK16 pKa = 10.42HH17 pKa = 6.15DD18 pKa = 4.02TGATFFDD25 pKa = 3.68GRR27 pKa = 11.84PAVFDD32 pKa = 3.71EE33 pKa = 4.56ADD35 pKa = 4.13FPAVAVYY42 pKa = 8.14LTGAEE47 pKa = 4.22YY48 pKa = 9.85TGEE51 pKa = 4.09EE52 pKa = 4.61LDD54 pKa = 3.6SDD56 pKa = 3.79TWQAEE61 pKa = 3.69LHH63 pKa = 6.35IEE65 pKa = 4.19VFLPAQVPDD74 pKa = 4.02SEE76 pKa = 4.66LDD78 pKa = 2.81AWMEE82 pKa = 3.93SRR84 pKa = 11.84IYY86 pKa = 10.51PVMSDD91 pKa = 3.25IPALSDD97 pKa = 5.02LITSMVASGYY107 pKa = 10.36DD108 pKa = 3.32YY109 pKa = 11.21RR110 pKa = 11.84RR111 pKa = 11.84DD112 pKa = 3.5DD113 pKa = 5.7DD114 pKa = 4.64AGLWSSADD122 pKa = 3.34LTYY125 pKa = 11.13VITYY129 pKa = 9.4EE130 pKa = 3.98MM131 pKa = 4.92

Molecular weight: 14.65 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1U8QQG3|A0A1U8QQG3_9CAUD Uncharacterized protein OS=Escherichia phage DE3 OX=482822 GN=ECD_10009 PE=4 SV=1
MM1 pKa = 7.24THH3 pKa = 6.13PHH5 pKa = 6.32DD6 pKa = 4.96NIRR9 pKa = 11.84VGAITFVYY17 pKa = 10.4SVTKK21 pKa = 10.38RR22 pKa = 11.84GWVFPGLSVIRR33 pKa = 11.84NPLKK37 pKa = 10.16AQRR40 pKa = 11.84LAEE43 pKa = 4.48EE44 pKa = 4.45INNKK48 pKa = 9.79RR49 pKa = 11.84GAVCTKK55 pKa = 10.48HH56 pKa = 6.68LLLSS60 pKa = 3.97

Molecular weight: 6.75 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

56

0

56

12245

28

1132

218.7

24.23

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

10.576 ± 0.801

1.151 ± 0.142

5.684 ± 0.235

6.59 ± 0.306

3.381 ± 0.245

6.974 ± 0.299

1.772 ± 0.177

4.933 ± 0.276

5.398 ± 0.333

7.603 ± 0.267

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.899 ± 0.155

3.969 ± 0.197

3.781 ± 0.244

4.475 ± 0.327

6.247 ± 0.289

6.827 ± 0.392

6.329 ± 0.358

6.803 ± 0.354

1.658 ± 0.175

2.948 ± 0.157
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski