Proteome-pI 2.0 - Proteome Isoelectric Point Database

Candidatus Symbiobacter mobilis CR

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Betaproteobacteria; Burkholderiales; Comamonadaceae; Candidatus Symbiobacter; Candidatus Symbiobacter mobilis

Average proteome isoelectric point is 6.73

Get precalculated fractions of proteins

Acidic

pI < 6.8

6.8-7.4

pI > 7.4

Basic

All

Virtual 2D-PAGE plot for 2609 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions

Protein with the lowest isoelectric point:

>tr|U5NDG2|U5NDG2_9BURK F420-non-reducing hydrogenase subunit G OS=Candidatus Symbiobacter mobilis CR OX=946483 GN=mvhG PE=4 SV=1

MM1 pKa = 8.33DD2 pKa = 4.2YY3 pKa = 11.1LGDD6 pKa = 3.86YY7 pKa = 9.95LGSIHH12 pKa = 7.29GSRR15 pKa = 11.84IDD17 pKa = 4.3GISAVPVADD26 pKa = 4.58GCCQPLCYY34 pKa = 9.72IATRR38 pKa = 11.84PVFSSTSCPVSHH50 pKa = 7.41PSDD53 pKa = 3.77PSGMADD59 pKa = 3.19SPAVLVVDD67 pKa = 5.41DD68 pKa = 5.95DD69 pKa = 5.42DD70 pKa = 4.47FTAQMTAMVLDD81 pKa = 4.02AAGFAAVTAEE91 pKa = 4.04GGIDD95 pKa = 3.52ALDD98 pKa = 3.67VVAQYY103 pKa = 10.68PSIGVVVSDD112 pKa = 4.45FEE114 pKa = 4.56MPFLDD119 pKa = 5.12GPGLYY124 pKa = 10.39AEE126 pKa = 5.06LRR128 pKa = 11.84EE129 pKa = 4.3QGYY132 pKa = 7.5QQPFVLLTGKK142 pKa = 10.25DD143 pKa = 3.41PAALSVLPPGISAVLTKK160 pKa = 10.15DD161 pKa = 3.11EE162 pKa = 4.82RR163 pKa = 11.84LSEE166 pKa = 4.38DD167 pKa = 3.18LPALVGEE174 pKa = 4.73LLRR177 pKa = 11.84GALGHH182 pKa = 5.98SS183 pKa = 3.86

Molecular weight: 18.99 kDa
Isoelectric point according different methods:

Protein with the highest isoelectric point:

>tr|U5NC20|U5NC20_9BURK Uncharacterized protein OS=Candidatus Symbiobacter mobilis CR OX=946483 GN=Cenrod_1649 PE=4 SV=1

MM1 pKa = 7.35KK2 pKa = 9.43RR3 pKa = 11.84TYY5 pKa = 10.07QPSKK9 pKa = 9.07IRR11 pKa = 11.84RR12 pKa = 11.84ARR14 pKa = 11.84THH16 pKa = 5.73GFRR19 pKa = 11.84VRR21 pKa = 11.84MRR23 pKa = 11.84THH25 pKa = 6.63SGRR28 pKa = 11.84AVINARR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 9.58GRR39 pKa = 11.84KK40 pKa = 8.75RR41 pKa = 11.84LAVV44 pKa = 3.41

Molecular weight: 5.23 kDa
Isoelectric point according different methods:

Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try ESI

ChTry ESI

ArgC ESI

LysN ESI

TryLysC ESI

Try MALDI

ChTry MALDI

ArgC MALDI

LysN MALDI

TryLysC MALDI

Try LTQ

ChTry LTQ

ArgC LTQ

LysN LTQ

TryLysC LTQ

Try MSlow

ChTry MSlow

ArgC MSlow

LysN MSlow

TryLysC MSlow

Try MShigh

ChTry MShigh

ArgC MShigh

LysN MShigh

TryLysC MShigh

General Statistics

Number of major isoforms	Number of additional isoforms	Number of all proteins	Number of amino acids	Min. Seq. Length	Max. Seq. Length	Avg. Seq. Length	Avg. Mol. Weight
2609	0	2609	902661	32	5992	346.0	37.94

Amino acid frequency

Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu
11.391 ± 0.067	1.189 ± 0.022	5.538 ± 0.038	5.477 ± 0.051	3.371 ± 0.033	7.55 ± 0.087	2.691 ± 0.03	4.674 ± 0.039	3.234 ± 0.05	10.698 ± 0.06

Met	Asn	Gln	Pro	Arg	Ser	Thr	Val	Trp	Tyr
2.389 ± 0.024	2.69 ± 0.032	5.037 ± 0.045	4.469 ± 0.044	6.91 ± 0.066	5.658 ± 0.052	5.585 ± 0.089	7.614 ± 0.053	1.493 ± 0.028	2.341 ± 0.028

Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here

See this proteome in:

Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski