Amino acid dipepetide frequency for Bosea sp. PAMC 26642

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	18.753AlaAla: 18.753 ± 0.15	1.136AlaCys: 1.136 ± 0.029	6.589AlaAsp: 6.589 ± 0.071	7.498AlaGlu: 7.498 ± 0.079	4.863AlaPhe: 4.863 ± 0.058	11.705AlaGly: 11.705 ± 0.105	2.213AlaHis: 2.213 ± 0.034	7.292AlaIle: 7.292 ± 0.074	4.445AlaLys: 4.445 ± 0.06	14.841AlaLeu: 14.841 ± 0.122	3.873AlaMet: 3.873 ± 0.047	2.792AlaAsn: 2.792 ± 0.044	5.909AlaPro: 5.909 ± 0.072	4.384AlaGln: 4.384 ± 0.054	9.483AlaArg: 9.483 ± 0.1	6.975AlaSer: 6.975 ± 0.075	6.684AlaThr: 6.684 ± 0.071	9.069AlaVal: 9.069 ± 0.084	1.534AlaTrp: 1.534 ± 0.032	2.564AlaTyr: 2.564 ± 0.046	0.0AlaXaa: 0.0 ± 0.0
Cys	0.96CysAla: 0.96 ± 0.027	0.101CysCys: 0.101 ± 0.008	0.475CysAsp: 0.475 ± 0.018	0.44CysGlu: 0.44 ± 0.016	0.313CysPhe: 0.313 ± 0.013	0.87CysGly: 0.87 ± 0.028	0.218CysHis: 0.218 ± 0.012	0.383CysIle: 0.383 ± 0.017	0.192CysLys: 0.192 ± 0.011	0.805CysLeu: 0.805 ± 0.025	0.132CysMet: 0.132 ± 0.01	0.176CysAsn: 0.176 ± 0.011	0.387CysPro: 0.387 ± 0.016	0.209CysGln: 0.209 ± 0.01	0.593CysArg: 0.593 ± 0.021	0.407CysSer: 0.407 ± 0.016	0.384CysThr: 0.384 ± 0.017	0.602CysVal: 0.602 ± 0.021	0.104CysTrp: 0.104 ± 0.007	0.167CysTyr: 0.167 ± 0.01	0.0CysXaa: 0.0 ± 0.0
Asp	7.068AspAla: 7.068 ± 0.076	0.429AspCys: 0.429 ± 0.018	2.963AspAsp: 2.963 ± 0.052	3.488AspGlu: 3.488 ± 0.045	2.065AspPhe: 2.065 ± 0.033	5.195AspGly: 5.195 ± 0.069	1.108AspHis: 1.108 ± 0.028	3.056AspIle: 3.056 ± 0.055	1.733AspLys: 1.733 ± 0.039	5.851AspLeu: 5.851 ± 0.072	1.232AspMet: 1.232 ± 0.027	1.093AspAsn: 1.093 ± 0.031	3.42AspPro: 3.42 ± 0.049	1.468AspGln: 1.468 ± 0.031	4.091AspArg: 4.091 ± 0.056	2.041AspSer: 2.041 ± 0.037	2.317AspThr: 2.317 ± 0.042	4.001AspVal: 4.001 ± 0.057	0.886AspTrp: 0.886 ± 0.025	1.312AspTyr: 1.312 ± 0.03	0.0AspXaa: 0.0 ± 0.0
Glu	8.086GluAla: 8.086 ± 0.09	0.321GluCys: 0.321 ± 0.014	2.379GluAsp: 2.379 ± 0.04	2.711GluGlu: 2.711 ± 0.05	1.654GluPhe: 1.654 ± 0.034	4.061GluGly: 4.061 ± 0.045	1.087GluHis: 1.087 ± 0.029	3.518GluIle: 3.518 ± 0.045	1.918GluLys: 1.918 ± 0.038	5.093GluLeu: 5.093 ± 0.061	1.502GluMet: 1.502 ± 0.032	1.282GluAsn: 1.282 ± 0.03	2.971GluPro: 2.971 ± 0.05	1.911GluGln: 1.911 ± 0.032	5.088GluArg: 5.088 ± 0.07	2.321GluSer: 2.321 ± 0.04	3.49GluThr: 3.49 ± 0.048	3.654GluVal: 3.654 ± 0.046	0.623GluTrp: 0.623 ± 0.02	0.779GluTyr: 0.779 ± 0.024	0.0GluXaa: 0.0 ± 0.0
Phe	4.924PheAla: 4.924 ± 0.059	0.365PheCys: 0.365 ± 0.014	2.524PheAsp: 2.524 ± 0.04	2.084PheGlu: 2.084 ± 0.038	1.322PhePhe: 1.322 ± 0.032	3.777PheGly: 3.777 ± 0.05	0.725PheHis: 0.725 ± 0.021	1.698PheIle: 1.698 ± 0.038	1.038PheLys: 1.038 ± 0.027	3.28PheLeu: 3.28 ± 0.055	0.805PheMet: 0.805 ± 0.022	0.977PheAsn: 0.977 ± 0.025	1.616PhePro: 1.616 ± 0.034	1.03PheGln: 1.03 ± 0.028	2.256PheArg: 2.256 ± 0.043	2.14PheSer: 2.14 ± 0.041	1.831PheThr: 1.831 ± 0.04	2.979PheVal: 2.979 ± 0.049	0.548PheTrp: 0.548 ± 0.017	0.829PheTyr: 0.829 ± 0.025	0.0PheXaa: 0.0 ± 0.0
Gly	9.793GlyAla: 9.793 ± 0.101	0.83GlyCys: 0.83 ± 0.024	4.226GlyAsp: 4.226 ± 0.061	4.918GlyGlu: 4.918 ± 0.067	3.702GlyPhe: 3.702 ± 0.049	7.531GlyGly: 7.531 ± 0.085	1.913GlyHis: 1.913 ± 0.038	4.594GlyIle: 4.594 ± 0.062	3.38GlyLys: 3.38 ± 0.055	9.867GlyLeu: 9.867 ± 0.094	2.183GlyMet: 2.183 ± 0.042	1.893GlyAsn: 1.893 ± 0.04	3.82GlyPro: 3.82 ± 0.056	3.023GlyGln: 3.023 ± 0.05	6.542GlyArg: 6.542 ± 0.067	4.83GlySer: 4.83 ± 0.061	4.425GlyThr: 4.425 ± 0.072	6.226GlyVal: 6.226 ± 0.064	1.374GlyTrp: 1.374 ± 0.032	2.184GlyTyr: 2.184 ± 0.042	0.0GlyXaa: 0.0 ± 0.0
His	2.289HisAla: 2.289 ± 0.039	0.199HisCys: 0.199 ± 0.011	1.207HisAsp: 1.207 ± 0.036	0.993HisGlu: 0.993 ± 0.025	0.766HisPhe: 0.766 ± 0.022	1.905HisGly: 1.905 ± 0.039	0.527HisHis: 0.527 ± 0.021	0.88HisIle: 0.88 ± 0.026	0.447HisLys: 0.447 ± 0.017	2.029HisLeu: 2.029 ± 0.04	0.416HisMet: 0.416 ± 0.015	0.39HisAsn: 0.39 ± 0.016	1.239HisPro: 1.239 ± 0.03	0.518HisGln: 0.518 ± 0.018	1.349HisArg: 1.349 ± 0.031	0.833HisSer: 0.833 ± 0.024	0.686HisThr: 0.686 ± 0.019	1.529HisVal: 1.529 ± 0.031	0.318HisTrp: 0.318 ± 0.014	0.48HisTyr: 0.48 ± 0.018	0.0HisXaa: 0.0 ± 0.0
Ile	8.278IleAla: 8.278 ± 0.07	0.51IleCys: 0.51 ± 0.02	3.645IleAsp: 3.645 ± 0.054	3.548IleGlu: 3.548 ± 0.054	1.638IlePhe: 1.638 ± 0.031	5.308IleGly: 5.308 ± 0.067	0.861IleHis: 0.861 ± 0.027	2.181IleIle: 2.181 ± 0.046	1.496IleLys: 1.496 ± 0.033	4.552IleLeu: 4.552 ± 0.062	0.989IleMet: 0.989 ± 0.026	1.223IleAsn: 1.223 ± 0.031	2.342IlePro: 2.342 ± 0.044	1.191IleGln: 1.191 ± 0.031	3.257IleArg: 3.257 ± 0.046	2.734IleSer: 2.734 ± 0.046	2.548IleThr: 2.548 ± 0.044	4.83IleVal: 4.83 ± 0.061	0.607IleTrp: 0.607 ± 0.02	1.018IleTyr: 1.018 ± 0.028	0.0IleXaa: 0.0 ± 0.0
Lys	4.688LysAla: 4.688 ± 0.068	0.128LysCys: 0.128 ± 0.009	1.681LysAsp: 1.681 ± 0.039	1.383LysGlu: 1.383 ± 0.031	0.868LysPhe: 0.868 ± 0.027	2.661LysGly: 2.661 ± 0.043	0.512LysHis: 0.512 ± 0.017	1.646LysIle: 1.646 ± 0.036	1.08LysLys: 1.08 ± 0.035	3.511LysLeu: 3.511 ± 0.055	0.65LysMet: 0.65 ± 0.022	0.779LysAsn: 0.779 ± 0.025	2.293LysPro: 2.293 ± 0.052	0.952LysGln: 0.952 ± 0.025	2.439LysArg: 2.439 ± 0.042	1.803LysSer: 1.803 ± 0.039	1.966LysThr: 1.966 ± 0.037	2.412LysVal: 2.412 ± 0.042	0.309LysTrp: 0.309 ± 0.013	0.504LysTyr: 0.504 ± 0.018	0.0LysXaa: 0.0 ± 0.0
Leu	15.18LeuAla: 15.18 ± 0.122	0.873LeuCys: 0.873 ± 0.026	6.333LeuAsp: 6.333 ± 0.078	4.78LeuGlu: 4.78 ± 0.059	3.568LeuPhe: 3.568 ± 0.059	8.914LeuGly: 8.914 ± 0.097	1.765LeuHis: 1.765 ± 0.041	5.269LeuIle: 5.269 ± 0.067	3.657LeuLys: 3.657 ± 0.056	9.707LeuLeu: 9.707 ± 0.112	2.341LeuMet: 2.341 ± 0.043	2.47LeuAsn: 2.47 ± 0.04	5.878LeuPro: 5.878 ± 0.067	2.692LeuGln: 2.692 ± 0.042	7.118LeuArg: 7.118 ± 0.078	6.505LeuSer: 6.505 ± 0.07	5.665LeuThr: 5.665 ± 0.074	8.132LeuVal: 8.132 ± 0.088	1.19LeuTrp: 1.19 ± 0.032	1.843LeuTyr: 1.843 ± 0.041	0.0LeuXaa: 0.0 ± 0.0
Met	3.226MetAla: 3.226 ± 0.044	0.139MetCys: 0.139 ± 0.009	1.029MetAsp: 1.029 ± 0.027	0.994MetGlu: 0.994 ± 0.027	0.663MetPhe: 0.663 ± 0.023	1.76MetGly: 1.76 ± 0.033	0.386MetHis: 0.386 ± 0.017	1.37MetIle: 1.37 ± 0.028	0.93MetLys: 0.93 ± 0.025	2.605MetLeu: 2.605 ± 0.041	0.632MetMet: 0.632 ± 0.023	0.708MetAsn: 0.708 ± 0.023	1.576MetPro: 1.576 ± 0.031	0.766MetGln: 0.766 ± 0.023	1.83MetArg: 1.83 ± 0.031	1.644MetSer: 1.644 ± 0.033	1.955MetThr: 1.955 ± 0.032	1.689MetVal: 1.689 ± 0.032	0.178MetTrp: 0.178 ± 0.01	0.252MetTyr: 0.252 ± 0.014	0.0MetXaa: 0.0 ± 0.0
Asn	3.121AsnAla: 3.121 ± 0.048	0.204AsnCys: 0.204 ± 0.012	1.201AsnAsp: 1.201 ± 0.034	1.138AsnGlu: 1.138 ± 0.029	0.836AsnPhe: 0.836 ± 0.024	2.162AsnGly: 2.162 ± 0.052	0.417AsnHis: 0.417 ± 0.015	1.112AsnIle: 1.112 ± 0.028	0.615AsnLys: 0.615 ± 0.021	2.496AsnLeu: 2.496 ± 0.039	0.517AsnMet: 0.517 ± 0.02	0.587AsnAsn: 0.587 ± 0.023	1.74AsnPro: 1.74 ± 0.033	0.663AsnGln: 0.663 ± 0.024	1.601AsnArg: 1.601 ± 0.032	1.093AsnSer: 1.093 ± 0.031	1.165AsnThr: 1.165 ± 0.03	1.724AsnVal: 1.724 ± 0.038	0.406AsnTrp: 0.406 ± 0.016	0.566AsnTyr: 0.566 ± 0.021	0.0AsnXaa: 0.0 ± 0.0
Pro	7.028ProAla: 7.028 ± 0.085	0.294ProCys: 0.294 ± 0.013	3.576ProAsp: 3.576 ± 0.056	3.473ProGlu: 3.473 ± 0.053	2.016ProPhe: 2.016 ± 0.036	4.813ProGly: 4.813 ± 0.059	1.049ProHis: 1.049 ± 0.029	2.414ProIle: 2.414 ± 0.045	1.689ProLys: 1.689 ± 0.037	4.935ProLeu: 4.935 ± 0.056	1.217ProMet: 1.217 ± 0.029	1.333ProAsn: 1.333 ± 0.034	2.728ProPro: 2.728 ± 0.059	1.751ProGln: 1.751 ± 0.036	3.24ProArg: 3.24 ± 0.056	2.863ProSer: 2.863 ± 0.042	2.628ProThr: 2.628 ± 0.035	4.354ProVal: 4.354 ± 0.054	0.684ProTrp: 0.684 ± 0.019	1.135ProTyr: 1.135 ± 0.026	0.0ProXaa: 0.0 ± 0.0
Gln	4.545GlnAla: 4.545 ± 0.057	0.184GlnCys: 0.184 ± 0.011	1.477GlnAsp: 1.477 ± 0.031	1.321GlnGlu: 1.321 ± 0.03	0.989GlnPhe: 0.989 ± 0.022	2.498GlnGly: 2.498 ± 0.044	0.581GlnHis: 0.581 ± 0.02	1.917GlnIle: 1.917 ± 0.035	0.974GlnLys: 0.974 ± 0.027	2.683GlnLeu: 2.683 ± 0.042	0.83GlnMet: 0.83 ± 0.024	0.8GlnAsn: 0.8 ± 0.023	1.799GlnPro: 1.799 ± 0.037	1.138GlnGln: 1.138 ± 0.031	2.57GlnArg: 2.57 ± 0.045	1.708GlnSer: 1.708 ± 0.033	1.714GlnThr: 1.714 ± 0.036	2.075GlnVal: 2.075 ± 0.039	0.356GlnTrp: 0.356 ± 0.016	0.493GlnTyr: 0.493 ± 0.02	0.0GlnXaa: 0.0 ± 0.0
Arg	8.218ArgAla: 8.218 ± 0.093	0.506ArgCys: 0.506 ± 0.017	4.137ArgAsp: 4.137 ± 0.052	4.2ArgGlu: 4.2 ± 0.06	3.056ArgPhe: 3.056 ± 0.044	4.957ArgGly: 4.957 ± 0.059	1.643ArgHis: 1.643 ± 0.036	4.232ArgIle: 4.232 ± 0.051	2.332ArgLys: 2.332 ± 0.044	8.351ArgLeu: 8.351 ± 0.092	1.898ArgMet: 1.898 ± 0.038	1.731ArgAsn: 1.731 ± 0.033	3.775ArgPro: 3.775 ± 0.059	2.607ArgGln: 2.607 ± 0.048	5.851ArgArg: 5.851 ± 0.087	3.854ArgSer: 3.854 ± 0.051	3.333ArgThr: 3.333 ± 0.047	4.76ArgVal: 4.76 ± 0.058	1.009ArgTrp: 1.009 ± 0.027	1.697ArgTyr: 1.697 ± 0.031	0.0ArgXaa: 0.0 ± 0.0
Ser	6.396SerAla: 6.396 ± 0.07	0.408SerCys: 0.408 ± 0.02	2.864SerAsp: 2.864 ± 0.046	2.748SerGlu: 2.748 ± 0.047	2.357SerPhe: 2.357 ± 0.035	5.471SerGly: 5.471 ± 0.063	1.044SerHis: 1.044 ± 0.027	2.687SerIle: 2.687 ± 0.041	1.517SerLys: 1.517 ± 0.03	5.716SerLeu: 5.716 ± 0.062	1.258SerMet: 1.258 ± 0.031	1.239SerAsn: 1.239 ± 0.03	2.841SerPro: 2.841 ± 0.043	1.672SerGln: 1.672 ± 0.036	3.754SerArg: 3.754 ± 0.057	2.831SerSer: 2.831 ± 0.047	2.655SerThr: 2.655 ± 0.05	4.128SerVal: 4.128 ± 0.053	0.766SerTrp: 0.766 ± 0.022	1.219SerTyr: 1.219 ± 0.027	0.0SerXaa: 0.0 ± 0.0
Thr	6.287ThrAla: 6.287 ± 0.07	0.377ThrCys: 0.377 ± 0.016	2.615ThrAsp: 2.615 ± 0.045	2.48ThrGlu: 2.48 ± 0.048	1.908ThrPhe: 1.908 ± 0.039	5.103ThrGly: 5.103 ± 0.064	0.988ThrHis: 0.988 ± 0.026	3.106ThrIle: 3.106 ± 0.053	1.461ThrLys: 1.461 ± 0.034	6.031ThrLeu: 6.031 ± 0.078	1.219ThrMet: 1.219 ± 0.029	1.159ThrAsn: 1.159 ± 0.035	3.306ThrPro: 3.306 ± 0.05	1.573ThrGln: 1.573 ± 0.036	3.432ThrArg: 3.432 ± 0.051	2.755ThrSer: 2.755 ± 0.048	2.987ThrThr: 2.987 ± 0.055	4.351ThrVal: 4.351 ± 0.064	0.588ThrTrp: 0.588 ± 0.02	1.05ThrTyr: 1.05 ± 0.027	0.0ThrXaa: 0.0 ± 0.0
Val	10.178ValAla: 10.178 ± 0.094	0.588ValCys: 0.588 ± 0.022	3.959ValAsp: 3.959 ± 0.049	4.591ValGlu: 4.591 ± 0.06	2.82ValPhe: 2.82 ± 0.044	5.685ValGly: 5.685 ± 0.07	1.273ValHis: 1.273 ± 0.026	4.0ValIle: 4.0 ± 0.056	2.364ValLys: 2.364 ± 0.045	7.635ValLeu: 7.635 ± 0.098	1.94ValMet: 1.94 ± 0.038	1.806ValAsn: 1.806 ± 0.039	3.788ValPro: 3.788 ± 0.055	1.986ValGln: 1.986 ± 0.038	4.824ValArg: 4.824 ± 0.068	4.421ValSer: 4.421 ± 0.057	4.621ValThr: 4.621 ± 0.064	6.436ValVal: 6.436 ± 0.073	0.843ValTrp: 0.843 ± 0.024	1.398ValTyr: 1.398 ± 0.033	0.0ValXaa: 0.0 ± 0.0
Trp	1.171TrpAla: 1.171 ± 0.034	0.132TrpCys: 0.132 ± 0.01	0.586TrpAsp: 0.586 ± 0.017	0.522TrpGlu: 0.522 ± 0.018	0.543TrpPhe: 0.543 ± 0.021	0.846TrpGly: 0.846 ± 0.026	0.302TrpHis: 0.302 ± 0.015	0.615TrpIle: 0.615 ± 0.02	0.43TrpLys: 0.43 ± 0.017	1.745TrpLeu: 1.745 ± 0.037	0.332TrpMet: 0.332 ± 0.014	0.415TrpAsn: 0.415 ± 0.015	0.714TrpPro: 0.714 ± 0.023	0.515TrpGln: 0.515 ± 0.018	1.171TrpArg: 1.171 ± 0.03	0.839TrpSer: 0.839 ± 0.023	0.803TrpThr: 0.803 ± 0.021	0.718TrpVal: 0.718 ± 0.024	0.224TrpTrp: 0.224 ± 0.013	0.256TrpTyr: 0.256 ± 0.015	0.0TrpXaa: 0.0 ± 0.0
Tyr	2.414TyrAla: 2.414 ± 0.042	0.197TyrCys: 0.197 ± 0.012	1.35TyrAsp: 1.35 ± 0.029	1.14TyrGlu: 1.14 ± 0.029	0.798TyrPhe: 0.798 ± 0.023	2.034TyrGly: 2.034 ± 0.042	0.388TyrHis: 0.388 ± 0.016	0.784TyrIle: 0.784 ± 0.021	0.567TyrLys: 0.567 ± 0.024	2.15TyrLeu: 2.15 ± 0.039	0.363TyrMet: 0.363 ± 0.015	0.51TyrAsn: 0.51 ± 0.018	1.08TyrPro: 1.08 ± 0.025	0.602TyrGln: 0.602 ± 0.018	1.656TyrArg: 1.656 ± 0.032	0.976TyrSer: 0.976 ± 0.028	0.92TyrThr: 0.92 ± 0.025	1.486TyrVal: 1.486 ± 0.032	0.332TyrTrp: 0.332 ± 0.015	0.463TyrTyr: 0.463 ± 0.02	0.0TyrXaa: 0.0 ± 0.0
Xaa	0.0XaaAla: 0.0 ± 0.0	0.0XaaCys: 0.0 ± 0.0	0.0XaaAsp: 0.0 ± 0.0	0.0XaaGlu: 0.0 ± 0.0	0.0XaaPhe: 0.0 ± 0.0	0.0XaaGly: 0.0 ± 0.0	0.0XaaHis: 0.0 ± 0.0	0.0XaaIle: 0.0 ± 0.0	0.0XaaLys: 0.0 ± 0.0	0.0XaaLeu: 0.0 ± 0.0	0.0XaaMet: 0.0 ± 0.0	0.0XaaAsn: 0.0 ± 0.0	0.0XaaPro: 0.0 ± 0.0	0.0XaaGln: 0.0 ± 0.0	0.0XaaArg: 0.0 ± 0.0	0.0XaaSer: 0.0 ± 0.0	0.0XaaThr: 0.0 ± 0.0	0.0XaaVal: 0.0 ± 0.0	0.0XaaTrp: 0.0 ± 0.0	0.0XaaTyr: 0.0 ± 0.0	0.0XaaXaa: 0.0 ± 0.0

Statistics based on 5070 proteins (1560634 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

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