Gracilibacillus kekensis

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Bacilli; Bacillales; Bacillaceae; Gracilibacillus

Average proteome isoelectric point is 6.09

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3690 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1M7QXB6|A0A1M7QXB6_9BACI Uncharacterized protein OS=Gracilibacillus kekensis OX=1027249 GN=SAMN05216179_3717 PE=4 SV=1
MM1 pKa = 7.55EE2 pKa = 5.46KK3 pKa = 10.46SKK5 pKa = 11.34SLLLLLFTLLFSVMLAACSDD25 pKa = 4.17DD26 pKa = 3.96SDD28 pKa = 4.47SSSSSEE34 pKa = 3.94GNGNDD39 pKa = 3.25SSSEE43 pKa = 4.14TEE45 pKa = 3.79SDD47 pKa = 3.2GGEE50 pKa = 3.68ASGDD54 pKa = 3.58SEE56 pKa = 4.51EE57 pKa = 4.8PYY59 pKa = 10.36EE60 pKa = 4.49ISIMTNAYY68 pKa = 8.46TPEE71 pKa = 4.75PPTPEE76 pKa = 3.91SPAWQAIEE84 pKa = 5.39DD85 pKa = 4.02FTNTEE90 pKa = 3.59LDD92 pKa = 3.07ITYY95 pKa = 9.87VPSSNYY101 pKa = 9.78DD102 pKa = 3.04EE103 pKa = 5.06RR104 pKa = 11.84FNITLASGDD113 pKa = 4.4LPSMILTNKK122 pKa = 8.89TSSFINAVNDD132 pKa = 3.85GAFWDD137 pKa = 3.99LTDD140 pKa = 4.7YY141 pKa = 11.58LDD143 pKa = 5.26DD144 pKa = 4.31YY145 pKa = 11.84EE146 pKa = 5.77NLSQMNDD153 pKa = 2.6IVKK156 pKa = 10.69NNISIDD162 pKa = 3.36GKK164 pKa = 10.66IYY166 pKa = 10.01GLYY169 pKa = 9.73RR170 pKa = 11.84ARR172 pKa = 11.84PLGRR176 pKa = 11.84MAVSIRR182 pKa = 11.84QDD184 pKa = 2.78WLDD187 pKa = 3.61NLGMEE192 pKa = 4.62TPTTTEE198 pKa = 3.18EE199 pKa = 4.8FYY201 pKa = 11.36DD202 pKa = 3.44VMYY205 pKa = 10.95AFTHH209 pKa = 7.29DD210 pKa = 4.67DD211 pKa = 3.64PDD213 pKa = 5.51GNGQDD218 pKa = 3.95DD219 pKa = 4.16TLGTIVSEE227 pKa = 4.82YY228 pKa = 9.57PGPWDD233 pKa = 5.1IMQTWFGVPNKK244 pKa = 9.5WGEE247 pKa = 4.19NEE249 pKa = 5.08DD250 pKa = 3.68GTLYY254 pKa = 10.32PYY256 pKa = 10.6FQDD259 pKa = 3.62PAYY262 pKa = 9.98KK263 pKa = 10.21EE264 pKa = 3.73ALDD267 pKa = 3.89YY268 pKa = 10.37FKK270 pKa = 11.14RR271 pKa = 11.84MYY273 pKa = 11.05DD274 pKa = 3.54DD275 pKa = 5.29GLVNDD280 pKa = 5.44DD281 pKa = 4.32FAVMDD286 pKa = 4.06PAKK289 pKa = 10.27WHH291 pKa = 6.41DD292 pKa = 3.46AYY294 pKa = 11.92VNGEE298 pKa = 4.03AGTVVDD304 pKa = 4.3VADD307 pKa = 3.74AASRR311 pKa = 11.84NRR313 pKa = 11.84DD314 pKa = 3.15KK315 pKa = 10.79MVEE318 pKa = 3.98ADD320 pKa = 3.69PSLEE324 pKa = 3.8GSVDD328 pKa = 3.21VFGAVEE334 pKa = 4.43GPNGLHH340 pKa = 6.63HH341 pKa = 7.2LPTSGYY347 pKa = 10.09NMMYY351 pKa = 10.24AISTTAVEE359 pKa = 4.58TEE361 pKa = 4.19EE362 pKa = 4.42EE363 pKa = 4.12LHH365 pKa = 7.04KK366 pKa = 10.91VLQFMDD372 pKa = 3.86DD373 pKa = 3.43MNTEE377 pKa = 4.25EE378 pKa = 4.34GQKK381 pKa = 10.41LGYY384 pKa = 10.24NGVEE388 pKa = 4.07GTHH391 pKa = 6.96YY392 pKa = 10.72EE393 pKa = 4.16MKK395 pKa = 10.51DD396 pKa = 3.36GEE398 pKa = 4.62FVPTTDD404 pKa = 2.7QALIYY409 pKa = 10.0EE410 pKa = 4.4YY411 pKa = 11.03EE412 pKa = 4.49DD413 pKa = 3.93LNQLLMFVPEE423 pKa = 4.38DD424 pKa = 3.71RR425 pKa = 11.84YY426 pKa = 9.74LTEE429 pKa = 4.73PVDD432 pKa = 4.58DD433 pKa = 3.88LTKK436 pKa = 10.58KK437 pKa = 10.31EE438 pKa = 4.11EE439 pKa = 3.95EE440 pKa = 4.02VLAEE444 pKa = 3.98NEE446 pKa = 4.32EE447 pKa = 4.38IVVGNPAEE455 pKa = 4.13PLVSEE460 pKa = 4.77VYY462 pKa = 10.28SRR464 pKa = 11.84QGQQLDD470 pKa = 4.21NIILDD475 pKa = 3.4ARR477 pKa = 11.84VQYY480 pKa = 10.53IVGQIDD486 pKa = 3.5EE487 pKa = 5.04AGLEE491 pKa = 4.13EE492 pKa = 6.06AEE494 pKa = 4.19QLWMNSGGKK503 pKa = 9.89EE504 pKa = 3.9YY505 pKa = 10.2IEE507 pKa = 5.52EE508 pKa = 4.04INQLYY513 pKa = 9.78QEE515 pKa = 4.42SQQ517 pKa = 2.89

Molecular weight: 57.95 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1M7QP32|A0A1M7QP32_9BACI Uncharacterized conserved protein YybS DUF2232 family OS=Gracilibacillus kekensis OX=1027249 GN=SAMN05216179_3417 PE=4 SV=1
MM1 pKa = 7.44KK2 pKa = 9.6RR3 pKa = 11.84TFQPNNRR10 pKa = 11.84KK11 pKa = 9.23RR12 pKa = 11.84KK13 pKa = 8.12KK14 pKa = 8.46VHH16 pKa = 5.5GFRR19 pKa = 11.84TRR21 pKa = 11.84MSTKK25 pKa = 10.06NGRR28 pKa = 11.84KK29 pKa = 8.75VLARR33 pKa = 11.84RR34 pKa = 11.84RR35 pKa = 11.84KK36 pKa = 9.2KK37 pKa = 10.05GRR39 pKa = 11.84KK40 pKa = 8.66VLSAA44 pKa = 4.05

Molecular weight: 5.31 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3690

0

3690

1118330

25

2738

303.1

34.24

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.449 ± 0.037

0.6 ± 0.011

5.591 ± 0.04

7.736 ± 0.053

4.587 ± 0.034

6.345 ± 0.042

2.119 ± 0.021

8.287 ± 0.042

6.667 ± 0.045

9.504 ± 0.047

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.682 ± 0.021

4.883 ± 0.031

3.433 ± 0.023

4.234 ± 0.033

3.672 ± 0.025

6.017 ± 0.03

5.554 ± 0.027

6.784 ± 0.029

1.126 ± 0.017

3.73 ± 0.03
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski