Mycobacterium phage Poenanya

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; Cheoctovirus; unclassified Cheoctovirus

Average proteome isoelectric point is 6.06

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 107 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A411AWY8|A0A411AWY8_9CAUD Lysin B OS=Mycobacterium phage Poenanya OX=2500793 GN=31 PE=4 SV=1
MM1 pKa = 7.38NATEE5 pKa = 5.72DD6 pKa = 3.71GLEE9 pKa = 4.22PLGEE13 pKa = 4.45APDD16 pKa = 4.23LGAPRR21 pKa = 11.84YY22 pKa = 9.14PMSAEE27 pKa = 4.13VTFHH31 pKa = 7.62RR32 pKa = 11.84ARR34 pKa = 11.84CTKK37 pKa = 10.5CGDD40 pKa = 3.08IEE42 pKa = 4.31TDD44 pKa = 3.37YY45 pKa = 11.76GDD47 pKa = 4.22FSAYY51 pKa = 10.11SDD53 pKa = 3.39PGGAINAVLAAADD66 pKa = 3.49WFGRR70 pKa = 11.84SAPTGEE76 pKa = 5.08LIDD79 pKa = 4.08FGGGRR84 pKa = 11.84MGQRR88 pKa = 11.84YY89 pKa = 7.81QLVEE93 pKa = 4.45LLCPDD98 pKa = 3.6CRR100 pKa = 11.84RR101 pKa = 11.84CEE103 pKa = 3.99VCGTAKK109 pKa = 10.44AYY111 pKa = 9.85PIDD114 pKa = 3.85DD115 pKa = 4.0HH116 pKa = 6.93LVCEE120 pKa = 4.29DD121 pKa = 4.12HH122 pKa = 7.29EE123 pKa = 4.31DD124 pKa = 3.94HH125 pKa = 6.79EE126 pKa = 5.25FEE128 pKa = 4.96AAPP131 pKa = 3.95

Molecular weight: 14.19 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A411AX56|A0A411AX56_9CAUD Uncharacterized protein OS=Mycobacterium phage Poenanya OX=2500793 GN=91 PE=4 SV=1
MM1 pKa = 6.68RR2 pKa = 11.84TAYY5 pKa = 9.46KK6 pKa = 10.35VRR8 pKa = 11.84AYY10 pKa = 9.99PDD12 pKa = 3.54AEE14 pKa = 3.94QAALLRR20 pKa = 11.84RR21 pKa = 11.84TFGCVRR27 pKa = 11.84LVWNKK32 pKa = 8.63TLAEE36 pKa = 3.94RR37 pKa = 11.84QQRR40 pKa = 11.84YY41 pKa = 5.17TTEE44 pKa = 4.09QKK46 pKa = 8.79STSYY50 pKa = 11.18KK51 pKa = 8.93EE52 pKa = 3.49TDD54 pKa = 3.15AALSEE59 pKa = 4.27WKK61 pKa = 8.75KK62 pKa = 9.31TEE64 pKa = 3.98DD65 pKa = 3.43LAFLSEE71 pKa = 4.44VSSVPLQQTLRR82 pKa = 11.84HH83 pKa = 4.8QHH85 pKa = 5.1SAFAAFFKK93 pKa = 11.02GLAKK97 pKa = 10.48YY98 pKa = 9.91PRR100 pKa = 11.84FKK102 pKa = 10.64SRR104 pKa = 11.84HH105 pKa = 4.52GRR107 pKa = 11.84QSAHH111 pKa = 4.97FTRR114 pKa = 11.84SAFRR118 pKa = 11.84IKK120 pKa = 10.73DD121 pKa = 3.4GALWLAKK128 pKa = 9.15TATPLRR134 pKa = 11.84IVWTWPGVDD143 pKa = 4.93LAALDD148 pKa = 3.81PTMVIVSRR156 pKa = 11.84EE157 pKa = 3.56PDD159 pKa = 3.33GRR161 pKa = 11.84WFVTFAVDD169 pKa = 4.75QPDD172 pKa = 4.38PQPLPATGEE181 pKa = 4.38SVGVDD186 pKa = 3.21LGIKK190 pKa = 10.29DD191 pKa = 4.22FATLSTGEE199 pKa = 4.36KK200 pKa = 9.27IANPRR205 pKa = 11.84HH206 pKa = 5.1MARR209 pKa = 11.84HH210 pKa = 4.89EE211 pKa = 4.24RR212 pKa = 11.84GLRR215 pKa = 11.84RR216 pKa = 11.84QQRR219 pKa = 11.84RR220 pKa = 11.84LSRR223 pKa = 11.84MKK225 pKa = 10.6NGSKK229 pKa = 10.03NRR231 pKa = 11.84ARR233 pKa = 11.84QRR235 pKa = 11.84VKK237 pKa = 10.29VARR240 pKa = 11.84KK241 pKa = 7.65HH242 pKa = 5.79ARR244 pKa = 11.84VRR246 pKa = 11.84DD247 pKa = 3.57ARR249 pKa = 11.84RR250 pKa = 11.84DD251 pKa = 3.87FLHH254 pKa = 6.02KK255 pKa = 9.64TSTEE259 pKa = 3.7LVRR262 pKa = 11.84RR263 pKa = 11.84FDD265 pKa = 4.03TIAVEE270 pKa = 5.02DD271 pKa = 4.2LAPKK275 pKa = 10.67NMVGNRR281 pKa = 11.84SLAKK285 pKa = 10.31SISEE289 pKa = 4.31CGWGEE294 pKa = 3.82FRR296 pKa = 11.84SMLEE300 pKa = 3.95YY301 pKa = 10.21KK302 pKa = 10.32AKK304 pKa = 9.93KK305 pKa = 8.98AGRR308 pKa = 11.84RR309 pKa = 11.84VAVINRR315 pKa = 11.84WYY317 pKa = 9.65PSSKK321 pKa = 8.31TCSACGHH328 pKa = 6.91LLATLSLGTRR338 pKa = 11.84HH339 pKa = 5.33WTCPDD344 pKa = 3.12CGTRR348 pKa = 11.84HH349 pKa = 6.6DD350 pKa = 4.59RR351 pKa = 11.84DD352 pKa = 3.06INAAKK357 pKa = 10.32NILVAAGLAEE367 pKa = 4.11TQNACGGDD375 pKa = 3.84VRR377 pKa = 11.84PHH379 pKa = 6.14GASHH383 pKa = 6.35RR384 pKa = 11.84QSPVKK389 pKa = 10.34QEE391 pKa = 3.98PSQATARR398 pKa = 11.84IPVLQGGEE406 pKa = 3.87

Molecular weight: 45.7 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

107

0

107

18568

30

1226

173.5

19.04

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

10.281 ± 0.428

1.217 ± 0.168

6.614 ± 0.236

5.886 ± 0.302

3.054 ± 0.189

8.945 ± 0.593

2.235 ± 0.171

4.346 ± 0.205

3.431 ± 0.205

7.281 ± 0.222

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.283 ± 0.115

3.388 ± 0.175

5.967 ± 0.19

3.414 ± 0.201

6.915 ± 0.45

5.897 ± 0.286

6.544 ± 0.266

7.184 ± 0.266

2.429 ± 0.146

2.687 ± 0.147
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski