Streptococcus phage Javan420

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; unclassified Siphoviridae

Average proteome isoelectric point is 6.46

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 73 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A4D6B3P6|A0A4D6B3P6_9CAUD Uncharacterized protein OS=Streptococcus phage Javan420 OX=2548157 GN=Javan420_0014 PE=4 SV=1
MM1 pKa = 8.48VEE3 pKa = 3.59IVLNGEE9 pKa = 4.64RR10 pKa = 11.84IDD12 pKa = 3.71SQSNFRR18 pKa = 11.84TSFLVAIDD26 pKa = 3.82FLRR29 pKa = 11.84HH30 pKa = 4.67EE31 pKa = 4.51QAEE34 pKa = 4.43SLHH37 pKa = 5.42EE38 pKa = 4.11LEE40 pKa = 5.9EE41 pKa = 4.12EE42 pKa = 4.24FEE44 pKa = 5.13DD45 pKa = 4.49YY46 pKa = 10.76TDD48 pKa = 4.18EE49 pKa = 6.22DD50 pKa = 3.96LMDD53 pKa = 4.44YY54 pKa = 10.65IEE56 pKa = 5.08NEE58 pKa = 3.78FDD60 pKa = 3.54VTPKK64 pKa = 10.53ILINRR69 pKa = 11.84ILDD72 pKa = 4.06SKK74 pKa = 9.38WQADD78 pKa = 3.93TQILDD83 pKa = 3.71DD84 pKa = 4.0

Molecular weight: 10.0 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A4D6B517|A0A4D6B517_9CAUD Uncharacterized protein OS=Streptococcus phage Javan420 OX=2548157 GN=Javan420_0046 PE=4 SV=1
MM1 pKa = 7.72HH2 pKa = 7.96DD3 pKa = 3.63WPLGPLTTSKK13 pKa = 10.84VATLGFGLVVNMSVGTVLSNLDD35 pKa = 2.84IDD37 pKa = 4.16LALVRR42 pKa = 11.84IVKK45 pKa = 10.02RR46 pKa = 11.84RR47 pKa = 11.84RR48 pKa = 3.11

Molecular weight: 5.26 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

73

0

73

13139

37

999

180.0

20.4

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.903 ± 0.51

0.616 ± 0.102

6.576 ± 0.317

7.847 ± 0.412

4.102 ± 0.173

6.028 ± 0.348

1.218 ± 0.1

7.268 ± 0.372

9.339 ± 0.418

8.25 ± 0.227

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.283 ± 0.163

5.678 ± 0.225

2.542 ± 0.241

3.608 ± 0.19

4.125 ± 0.253

6.294 ± 0.27

5.891 ± 0.261

6.325 ± 0.287

1.233 ± 0.167

3.874 ± 0.353
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski