Amino acid dipepetide frequency for Pseudomonas phage 8P

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
17.042AlaAla: 17.042 ± 1.692
0.837AlaCys: 0.837 ± 0.336
8.619AlaAsp: 8.619 ± 0.786
10.54AlaGlu: 10.54 ± 0.887
3.448AlaPhe: 3.448 ± 0.582
9.161AlaGly: 9.161 ± 1.075
2.019AlaHis: 2.019 ± 0.278
5.122AlaIle: 5.122 ± 0.482
6.452AlaLys: 6.452 ± 1.224
9.358AlaLeu: 9.358 ± 1.002
3.399AlaMet: 3.399 ± 0.375
3.645AlaAsn: 3.645 ± 0.377
7.339AlaPro: 7.339 ± 0.83
5.615AlaGln: 5.615 ± 0.648
9.26AlaArg: 9.26 ± 0.841
6.699AlaSer: 6.699 ± 0.602
7.437AlaThr: 7.437 ± 0.693
7.684AlaVal: 7.684 ± 0.517
1.478AlaTrp: 1.478 ± 0.323
2.61AlaTyr: 2.61 ± 0.465
0.0AlaXaa: 0.0 ± 0.0
Cys
0.493CysAla: 0.493 ± 0.295
0.394CysCys: 0.394 ± 0.192
0.591CysAsp: 0.591 ± 0.218
0.197CysGlu: 0.197 ± 0.119
0.246CysPhe: 0.246 ± 0.16
1.231CysGly: 1.231 ± 0.478
0.296CysHis: 0.296 ± 0.166
0.345CysIle: 0.345 ± 0.16
0.345CysLys: 0.345 ± 0.153
0.64CysLeu: 0.64 ± 0.241
0.296CysMet: 0.296 ± 0.171
0.394CysAsn: 0.394 ± 0.19
0.246CysPro: 0.246 ± 0.146
0.345CysGln: 0.345 ± 0.221
0.542CysArg: 0.542 ± 0.271
0.246CysSer: 0.246 ± 0.127
0.394CysThr: 0.394 ± 0.158
0.345CysVal: 0.345 ± 0.189
0.148CysTrp: 0.148 ± 0.108
0.246CysTyr: 0.246 ± 0.13
0.0CysXaa: 0.0 ± 0.0
Asp
9.013AspAla: 9.013 ± 0.753
0.394AspCys: 0.394 ± 0.185
3.694AspAsp: 3.694 ± 0.451
3.743AspGlu: 3.743 ± 0.484
2.069AspPhe: 2.069 ± 0.408
5.024AspGly: 5.024 ± 0.573
1.084AspHis: 1.084 ± 0.282
2.906AspIle: 2.906 ± 0.339
3.3AspLys: 3.3 ± 0.491
4.778AspLeu: 4.778 ± 0.412
1.428AspMet: 1.428 ± 0.271
1.281AspAsn: 1.281 ± 0.26
2.857AspPro: 2.857 ± 0.296
3.399AspGln: 3.399 ± 0.376
2.906AspArg: 2.906 ± 0.261
3.349AspSer: 3.349 ± 0.402
3.349AspThr: 3.349 ± 0.393
3.645AspVal: 3.645 ± 0.52
0.936AspTrp: 0.936 ± 0.214
1.822AspTyr: 1.822 ± 0.334
0.0AspXaa: 0.0 ± 0.0
Glu
9.654GluAla: 9.654 ± 0.842
0.443GluCys: 0.443 ± 0.232
3.842GluAsp: 3.842 ± 0.449
2.906GluGlu: 2.906 ± 0.503
2.019GluPhe: 2.019 ± 0.301
4.778GluGly: 4.778 ± 0.465
1.576GluHis: 1.576 ± 0.36
3.103GluIle: 3.103 ± 0.38
2.66GluLys: 2.66 ± 0.372
7.142GluLeu: 7.142 ± 0.571
1.478GluMet: 1.478 ± 0.23
1.921GluAsn: 1.921 ± 0.266
3.497GluPro: 3.497 ± 0.503
4.63GluGln: 4.63 ± 0.619
5.812GluArg: 5.812 ± 0.684
3.94GluSer: 3.94 ± 0.415
2.906GluThr: 2.906 ± 0.518
3.546GluVal: 3.546 ± 0.506
1.084GluTrp: 1.084 ± 0.231
2.167GluTyr: 2.167 ± 0.328
0.0GluXaa: 0.0 ± 0.0
Phe
3.645PheAla: 3.645 ± 0.508
0.148PheCys: 0.148 ± 0.076
2.807PheAsp: 2.807 ± 0.465
2.118PheGlu: 2.118 ± 0.406
0.887PhePhe: 0.887 ± 0.218
3.349PheGly: 3.349 ± 0.598
0.69PheHis: 0.69 ± 0.249
1.281PheIle: 1.281 ± 0.226
1.97PheLys: 1.97 ± 0.319
2.364PheLeu: 2.364 ± 0.436
0.64PheMet: 0.64 ± 0.136
0.887PheAsn: 0.887 ± 0.197
1.675PhePro: 1.675 ± 0.245
1.33PheGln: 1.33 ± 0.281
1.921PheArg: 1.921 ± 0.523
2.266PheSer: 2.266 ± 0.262
1.675PheThr: 1.675 ± 0.27
1.281PheVal: 1.281 ± 0.254
0.591PheTrp: 0.591 ± 0.174
0.64PheTyr: 0.64 ± 0.197
0.0PheXaa: 0.0 ± 0.0
Gly
8.275GlyAla: 8.275 ± 0.874
0.64GlyCys: 0.64 ± 0.313
4.778GlyAsp: 4.778 ± 0.417
5.418GlyGlu: 5.418 ± 0.512
3.201GlyPhe: 3.201 ± 0.578
5.615GlyGly: 5.615 ± 0.748
1.379GlyHis: 1.379 ± 0.233
4.482GlyIle: 4.482 ± 0.763
3.596GlyLys: 3.596 ± 0.486
5.024GlyLeu: 5.024 ± 0.575
2.216GlyMet: 2.216 ± 0.393
2.561GlyAsn: 2.561 ± 0.455
2.66GlyPro: 2.66 ± 0.336
3.399GlyGln: 3.399 ± 0.477
4.482GlyArg: 4.482 ± 0.446
4.285GlySer: 4.285 ± 0.576
5.172GlyThr: 5.172 ± 0.441
5.467GlyVal: 5.467 ± 0.537
1.182GlyTrp: 1.182 ± 0.196
3.054GlyTyr: 3.054 ± 0.512
0.0GlyXaa: 0.0 ± 0.0
His
1.97HisAla: 1.97 ± 0.331
0.246HisCys: 0.246 ± 0.16
1.034HisAsp: 1.034 ± 0.301
0.985HisGlu: 0.985 ± 0.191
0.887HisPhe: 0.887 ± 0.294
1.576HisGly: 1.576 ± 0.344
0.69HisHis: 0.69 ± 0.224
0.591HisIle: 0.591 ± 0.189
0.788HisLys: 0.788 ± 0.21
1.231HisLeu: 1.231 ± 0.296
0.542HisMet: 0.542 ± 0.183
0.64HisAsn: 0.64 ± 0.189
1.084HisPro: 1.084 ± 0.3
0.887HisGln: 0.887 ± 0.201
1.872HisArg: 1.872 ± 0.453
0.837HisSer: 0.837 ± 0.205
1.428HisThr: 1.428 ± 0.405
1.084HisVal: 1.084 ± 0.274
0.345HisTrp: 0.345 ± 0.121
1.084HisTyr: 1.084 ± 0.283
0.0HisXaa: 0.0 ± 0.0
Ile
7.191IleAla: 7.191 ± 0.74
0.246IleCys: 0.246 ± 0.143
3.349IleAsp: 3.349 ± 0.361
3.793IleGlu: 3.793 ± 0.51
1.231IlePhe: 1.231 ± 0.231
2.906IleGly: 2.906 ± 0.349
1.133IleHis: 1.133 ± 0.212
1.625IleIle: 1.625 ± 0.315
2.364IleLys: 2.364 ± 0.308
2.512IleLeu: 2.512 ± 0.361
0.837IleMet: 0.837 ± 0.198
1.822IleAsn: 1.822 ± 0.33
3.201IlePro: 3.201 ± 0.347
1.527IleGln: 1.527 ± 0.263
2.709IleArg: 2.709 ± 0.333
1.625IleSer: 1.625 ± 0.284
3.251IleThr: 3.251 ± 0.338
2.512IleVal: 2.512 ± 0.471
0.345IleTrp: 0.345 ± 0.151
0.591IleTyr: 0.591 ± 0.19
0.0IleXaa: 0.0 ± 0.0
Lys
7.142LysAla: 7.142 ± 0.981
0.394LysCys: 0.394 ± 0.201
2.364LysAsp: 2.364 ± 0.336
2.167LysGlu: 2.167 ± 0.302
1.084LysPhe: 1.084 ± 0.258
3.743LysGly: 3.743 ± 0.491
0.64LysHis: 0.64 ± 0.306
2.315LysIle: 2.315 ± 0.394
2.216LysLys: 2.216 ± 0.4
3.448LysLeu: 3.448 ± 0.409
1.034LysMet: 1.034 ± 0.361
1.379LysAsn: 1.379 ± 0.254
3.891LysPro: 3.891 ± 0.393
2.118LysGln: 2.118 ± 0.344
3.743LysArg: 3.743 ± 0.564
2.069LysSer: 2.069 ± 0.295
2.512LysThr: 2.512 ± 0.311
3.103LysVal: 3.103 ± 0.338
0.345LysTrp: 0.345 ± 0.106
1.084LysTyr: 1.084 ± 0.217
0.0LysXaa: 0.0 ± 0.0
Leu
9.457LeuAla: 9.457 ± 0.666
0.69LeuCys: 0.69 ± 0.289
4.63LeuAsp: 4.63 ± 0.569
5.27LeuGlu: 5.27 ± 0.525
2.61LeuPhe: 2.61 ± 0.344
4.925LeuGly: 4.925 ± 0.499
1.625LeuHis: 1.625 ± 0.241
3.596LeuIle: 3.596 ± 0.497
3.004LeuLys: 3.004 ± 0.359
4.876LeuLeu: 4.876 ± 0.394
2.266LeuMet: 2.266 ± 0.27
2.857LeuAsn: 2.857 ± 0.391
4.63LeuPro: 4.63 ± 0.62
2.66LeuGln: 2.66 ± 0.373
5.812LeuArg: 5.812 ± 0.552
3.891LeuSer: 3.891 ± 0.417
4.63LeuThr: 4.63 ± 0.515
4.482LeuVal: 4.482 ± 0.673
0.788LeuTrp: 0.788 ± 0.174
1.921LeuTyr: 1.921 ± 0.357
0.0LeuXaa: 0.0 ± 0.0
Met
2.955MetAla: 2.955 ± 0.405
0.148MetCys: 0.148 ± 0.109
1.084MetAsp: 1.084 ± 0.298
1.231MetGlu: 1.231 ± 0.281
0.64MetPhe: 0.64 ± 0.151
2.315MetGly: 2.315 ± 0.333
0.345MetHis: 0.345 ± 0.144
0.887MetIle: 0.887 ± 0.197
1.675MetLys: 1.675 ± 0.3
1.527MetLeu: 1.527 ± 0.299
0.64MetMet: 0.64 ± 0.144
0.739MetAsn: 0.739 ± 0.15
1.773MetPro: 1.773 ± 0.311
1.281MetGln: 1.281 ± 0.313
1.773MetArg: 1.773 ± 0.26
1.478MetSer: 1.478 ± 0.266
1.97MetThr: 1.97 ± 0.296
1.773MetVal: 1.773 ± 0.26
0.443MetTrp: 0.443 ± 0.134
0.64MetTyr: 0.64 ± 0.172
0.0MetXaa: 0.0 ± 0.0
Asn
3.694AsnAla: 3.694 ± 0.428
0.296AsnCys: 0.296 ± 0.133
1.379AsnAsp: 1.379 ± 0.214
2.216AsnGlu: 2.216 ± 0.331
1.034AsnPhe: 1.034 ± 0.261
3.251AsnGly: 3.251 ± 0.476
0.443AsnHis: 0.443 ± 0.168
0.788AsnIle: 0.788 ± 0.221
1.379AsnLys: 1.379 ± 0.272
2.709AsnLeu: 2.709 ± 0.334
0.591AsnMet: 0.591 ± 0.163
0.837AsnAsn: 0.837 ± 0.224
1.921AsnPro: 1.921 ± 0.303
1.231AsnGln: 1.231 ± 0.318
2.118AsnArg: 2.118 ± 0.328
2.315AsnSer: 2.315 ± 0.317
1.773AsnThr: 1.773 ± 0.319
1.675AsnVal: 1.675 ± 0.341
0.542AsnTrp: 0.542 ± 0.176
1.084AsnTyr: 1.084 ± 0.26
0.0AsnXaa: 0.0 ± 0.0
Pro
8.176ProAla: 8.176 ± 0.773
0.591ProCys: 0.591 ± 0.245
4.088ProAsp: 4.088 ± 0.677
4.975ProGlu: 4.975 ± 0.535
1.872ProPhe: 1.872 ± 0.31
3.793ProGly: 3.793 ± 0.483
0.936ProHis: 0.936 ± 0.298
2.118ProIle: 2.118 ± 0.389
2.216ProLys: 2.216 ± 0.313
3.349ProLeu: 3.349 ± 0.513
1.675ProMet: 1.675 ± 0.298
1.872ProAsn: 1.872 ± 0.295
2.807ProPro: 2.807 ± 0.753
2.463ProGln: 2.463 ± 0.415
2.512ProArg: 2.512 ± 0.455
3.201ProSer: 3.201 ± 0.356
3.3ProThr: 3.3 ± 0.332
3.891ProVal: 3.891 ± 0.46
0.788ProTrp: 0.788 ± 0.189
1.33ProTyr: 1.33 ± 0.407
0.0ProXaa: 0.0 ± 0.0
Gln
6.502GlnAla: 6.502 ± 0.644
0.197GlnCys: 0.197 ± 0.118
1.97GlnAsp: 1.97 ± 0.31
3.546GlnGlu: 3.546 ± 0.462
1.527GlnPhe: 1.527 ± 0.234
2.955GlnGly: 2.955 ± 0.403
0.936GlnHis: 0.936 ± 0.268
2.512GlnIle: 2.512 ± 0.348
1.478GlnLys: 1.478 ± 0.323
3.546GlnLeu: 3.546 ± 0.395
1.133GlnMet: 1.133 ± 0.254
1.281GlnAsn: 1.281 ± 0.332
2.66GlnPro: 2.66 ± 0.527
2.955GlnGln: 2.955 ± 0.472
3.349GlnArg: 3.349 ± 0.341
2.069GlnSer: 2.069 ± 0.357
1.921GlnThr: 1.921 ± 0.289
2.955GlnVal: 2.955 ± 0.404
0.69GlnTrp: 0.69 ± 0.229
1.428GlnTyr: 1.428 ± 0.215
0.0GlnXaa: 0.0 ± 0.0
Arg
7.388ArgAla: 7.388 ± 0.567
0.837ArgCys: 0.837 ± 0.34
3.891ArgAsp: 3.891 ± 0.413
5.172ArgGlu: 5.172 ± 0.659
2.413ArgPhe: 2.413 ± 0.37
3.94ArgGly: 3.94 ± 0.548
1.625ArgHis: 1.625 ± 0.452
3.546ArgIle: 3.546 ± 0.455
3.349ArgLys: 3.349 ± 0.438
5.96ArgLeu: 5.96 ± 0.667
1.576ArgMet: 1.576 ± 0.28
2.069ArgAsn: 2.069 ± 0.307
2.906ArgPro: 2.906 ± 0.585
3.3ArgGln: 3.3 ± 0.313
4.088ArgArg: 4.088 ± 0.641
3.399ArgSer: 3.399 ± 0.433
3.596ArgThr: 3.596 ± 0.5
5.024ArgVal: 5.024 ± 0.529
0.985ArgTrp: 0.985 ± 0.309
2.118ArgTyr: 2.118 ± 0.451
0.0ArgXaa: 0.0 ± 0.0
Ser
5.763SerAla: 5.763 ± 0.663
0.246SerCys: 0.246 ± 0.121
2.512SerAsp: 2.512 ± 0.302
3.694SerGlu: 3.694 ± 0.398
1.773SerPhe: 1.773 ± 0.264
5.122SerGly: 5.122 ± 0.433
1.379SerHis: 1.379 ± 0.334
2.266SerIle: 2.266 ± 0.387
2.955SerLys: 2.955 ± 0.472
4.334SerLeu: 4.334 ± 0.351
1.281SerMet: 1.281 ± 0.166
1.625SerAsn: 1.625 ± 0.388
2.955SerPro: 2.955 ± 0.423
1.97SerGln: 1.97 ± 0.229
2.561SerArg: 2.561 ± 0.411
2.857SerSer: 2.857 ± 0.385
2.955SerThr: 2.955 ± 0.4
3.645SerVal: 3.645 ± 0.451
0.788SerTrp: 0.788 ± 0.191
1.478SerTyr: 1.478 ± 0.277
0.0SerXaa: 0.0 ± 0.0
Thr
6.797ThrAla: 6.797 ± 0.679
0.296ThrCys: 0.296 ± 0.178
4.334ThrAsp: 4.334 ± 0.579
3.645ThrGlu: 3.645 ± 0.435
1.724ThrPhe: 1.724 ± 0.315
5.122ThrGly: 5.122 ± 0.625
0.985ThrHis: 0.985 ± 0.219
2.512ThrIle: 2.512 ± 0.381
2.512ThrLys: 2.512 ± 0.338
4.778ThrLeu: 4.778 ± 0.479
1.379ThrMet: 1.379 ± 0.215
2.216ThrAsn: 2.216 ± 0.239
4.285ThrPro: 4.285 ± 0.618
2.364ThrGln: 2.364 ± 0.272
3.645ThrArg: 3.645 ± 0.598
2.66ThrSer: 2.66 ± 0.455
3.448ThrThr: 3.448 ± 0.627
3.546ThrVal: 3.546 ± 0.39
0.887ThrTrp: 0.887 ± 0.276
1.281ThrTyr: 1.281 ± 0.236
0.0ThrXaa: 0.0 ± 0.0
Val
8.275ValAla: 8.275 ± 0.736
0.542ValCys: 0.542 ± 0.268
3.152ValAsp: 3.152 ± 0.509
5.073ValGlu: 5.073 ± 0.793
2.069ValPhe: 2.069 ± 0.24
4.728ValGly: 4.728 ± 0.512
0.739ValHis: 0.739 ± 0.161
2.709ValIle: 2.709 ± 0.321
3.201ValLys: 3.201 ± 0.32
4.039ValLeu: 4.039 ± 0.497
1.724ValMet: 1.724 ± 0.322
1.527ValAsn: 1.527 ± 0.283
3.694ValPro: 3.694 ± 0.466
2.709ValGln: 2.709 ± 0.385
4.482ValArg: 4.482 ± 0.605
3.054ValSer: 3.054 ± 0.384
4.384ValThr: 4.384 ± 0.498
4.039ValVal: 4.039 ± 0.456
0.985ValTrp: 0.985 ± 0.271
1.33ValTyr: 1.33 ± 0.265
0.0ValXaa: 0.0 ± 0.0
Trp
1.33TrpAla: 1.33 ± 0.242
0.049TrpCys: 0.049 ± 0.051
0.739TrpAsp: 0.739 ± 0.144
0.837TrpGlu: 0.837 ± 0.189
0.246TrpPhe: 0.246 ± 0.123
1.231TrpGly: 1.231 ± 0.256
0.542TrpHis: 0.542 ± 0.173
0.69TrpIle: 0.69 ± 0.165
0.394TrpLys: 0.394 ± 0.141
1.133TrpLeu: 1.133 ± 0.31
0.394TrpMet: 0.394 ± 0.125
0.739TrpAsn: 0.739 ± 0.193
0.69TrpPro: 0.69 ± 0.248
0.69TrpGln: 0.69 ± 0.182
1.281TrpArg: 1.281 ± 0.212
0.69TrpSer: 0.69 ± 0.136
0.936TrpThr: 0.936 ± 0.225
0.887TrpVal: 0.887 ± 0.153
0.296TrpTrp: 0.296 ± 0.112
0.345TrpTyr: 0.345 ± 0.153
0.0TrpXaa: 0.0 ± 0.0
Tyr
3.004TyrAla: 3.004 ± 0.399
0.345TyrCys: 0.345 ± 0.183
2.118TyrAsp: 2.118 ± 0.354
1.576TyrGlu: 1.576 ± 0.233
1.182TyrPhe: 1.182 ± 0.237
2.118TyrGly: 2.118 ± 0.522
0.739TyrHis: 0.739 ± 0.326
1.379TyrIle: 1.379 ± 0.238
0.936TyrLys: 0.936 ± 0.267
1.872TyrLeu: 1.872 ± 0.322
0.739TyrMet: 0.739 ± 0.207
0.985TyrAsn: 0.985 ± 0.171
1.182TyrPro: 1.182 ± 0.199
0.788TyrGln: 0.788 ± 0.223
2.315TyrArg: 2.315 ± 0.309
1.231TyrSer: 1.231 ± 0.227
1.379TyrThr: 1.379 ± 0.289
1.872TyrVal: 1.872 ± 0.526
0.493TyrTrp: 0.493 ± 0.203
0.394TyrTyr: 0.394 ± 0.169
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 63 proteins (20304 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski