Spodoptera frugiperda rhabdovirus
Average proteome isoelectric point is 6.7
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 6 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|X2KYE6|X2KYE6_9RHAB Uncharacterized protein OS=Spodoptera frugiperda rhabdovirus OX=1481139 PE=4 SV=1
MM1 pKa = 7.01 TQGTMKK7 pKa = 10.35 PVWEE11 pKa = 4.04 EE12 pKa = 3.76 LGTGEE17 pKa = 4.96 TEE19 pKa = 4.07 FQGTVDD25 pKa = 3.0 IPGRR29 pKa = 11.84 SLKK32 pKa = 10.25 PEE34 pKa = 3.87 KK35 pKa = 9.96 TDD37 pKa = 3.12 WSVDD41 pKa = 3.2 TCRR44 pKa = 11.84 EE45 pKa = 3.23 ISLNLKK51 pKa = 10.35 LPGEE55 pKa = 4.61 IWQLAHH61 pKa = 6.75 QEE63 pKa = 4.4 TIFNRR68 pKa = 11.84 FLTFYY73 pKa = 10.09 ATGYY77 pKa = 9.71 VPNTHH82 pKa = 6.43 TATEE86 pKa = 4.42 IVLSMASLIFKK97 pKa = 10.25 DD98 pKa = 3.65 KK99 pKa = 11.13 AKK101 pKa = 11.04 APIDD105 pKa = 4.83 LIWDD109 pKa = 3.85 DD110 pKa = 4.55 SFQASPSEE118 pKa = 4.02 EE119 pKa = 4.07 CGFSVVGEE127 pKa = 4.3 TPLVIGQHH135 pKa = 6.76 PDD137 pKa = 3.19 DD138 pKa = 5.56 DD139 pKa = 5.84 DD140 pKa = 3.75 YY141 pKa = 11.95 TLRR144 pKa = 11.84 EE145 pKa = 4.33 DD146 pKa = 4.02 EE147 pKa = 4.92 EE148 pKa = 4.45 SAAMNEE154 pKa = 4.12 EE155 pKa = 4.17 EE156 pKa = 5.28 KK157 pKa = 10.73 IQAALKK163 pKa = 9.35 TLGIQDD169 pKa = 3.94 TPVDD173 pKa = 4.26 LKK175 pKa = 10.9 DD176 pKa = 3.17 ASGIVFEE183 pKa = 4.49 TKK185 pKa = 9.46 EE186 pKa = 4.19 DD187 pKa = 3.51 RR188 pKa = 11.84 EE189 pKa = 3.94 QRR191 pKa = 11.84 IKK193 pKa = 11.05 NEE195 pKa = 3.93 KK196 pKa = 9.19 ALHH199 pKa = 5.75 VEE201 pKa = 4.02 DD202 pKa = 6.23 DD203 pKa = 3.94 INALTQITKK212 pKa = 10.25 QFLFEE217 pKa = 4.23 YY218 pKa = 9.73 STGSLQKK225 pKa = 10.07 FVAKK229 pKa = 9.37 ATTIFIDD236 pKa = 3.61 NNATNGFTRR245 pKa = 11.84 LHH247 pKa = 5.53 LHH249 pKa = 7.77 AIRR252 pKa = 11.84 VMNFIALTMLRR263 pKa = 11.84 KK264 pKa = 8.3 VTKK267 pKa = 10.66 SNAQMINAFLKK278 pKa = 9.84 EE279 pKa = 3.9 QYY281 pKa = 9.71 KK282 pKa = 10.83 RR283 pKa = 11.84 NIASLIPGALSSDD296 pKa = 4.31 FAPPSKK302 pKa = 10.86 SCIDD306 pKa = 3.43 KK307 pKa = 9.33 LTAISKK313 pKa = 10.11 NDD315 pKa = 3.75 PAVSSFFAKK324 pKa = 9.71 VVMLNMEE331 pKa = 4.31 EE332 pKa = 4.31 EE333 pKa = 4.14 RR334 pKa = 11.84 RR335 pKa = 11.84 NPSLVACLGASLLTHH350 pKa = 5.76 TTWNGMGILHH360 pKa = 6.71 VIFEE364 pKa = 4.72 VCLFHH369 pKa = 7.16 QISWKK374 pKa = 10.38 RR375 pKa = 11.84 LVTEE379 pKa = 4.6 SLTSLTKK386 pKa = 9.91 MSWGEE391 pKa = 3.72 VSQFLIKK398 pKa = 10.57 YY399 pKa = 6.17 QAKK402 pKa = 9.88 GNPDD406 pKa = 3.3 PTVAWARR413 pKa = 11.84 IIDD416 pKa = 3.74 DD417 pKa = 4.15 SYY419 pKa = 11.8 FMRR422 pKa = 11.84 LTIVNHH428 pKa = 5.63 PTLAALLVEE437 pKa = 4.3 SLIRR441 pKa = 11.84 SQKK444 pKa = 10.92 DD445 pKa = 3.27 DD446 pKa = 4.72 GILNANWAIQHH457 pKa = 6.93 RR458 pKa = 11.84 DD459 pKa = 3.18 TINYY463 pKa = 8.66 YY464 pKa = 10.39 RR465 pKa = 11.84 DD466 pKa = 3.34 AAKK469 pKa = 10.85 LLTDD473 pKa = 3.79 KK474 pKa = 10.65 LTGQTATVQALTNEE488 pKa = 4.17 AADD491 pKa = 4.38 LVRR494 pKa = 11.84 TMNAGPSRR502 pKa = 11.84 YY503 pKa = 9.04 HH504 pKa = 6.42 PRR506 pKa = 11.84 PSTLIPMVDD515 pKa = 4.65 LNPEE519 pKa = 4.09 DD520 pKa = 3.63 LL521 pKa = 4.71
Molecular weight: 58.39 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 5.244
IPC2_protein 5.219
IPC_protein 5.207
Toseland 5.258
ProMoST 5.385
Dawson 5.258
Bjellqvist 5.359
Wikipedia 5.156
Rodwell 5.181
Grimsley 5.219
Solomon 5.258
Lehninger 5.219
Nozaki 5.397
DTASelect 5.563
Thurlkill 5.283
EMBOSS 5.258
Sillero 5.474
Patrickios 4.304
IPC_peptide 5.258
IPC2_peptide 5.474
IPC2.peptide.svr19 5.407
Protein with the highest isoelectric point:
>tr|X2L5J8|X2L5J8_9RHAB P OS=Spodoptera frugiperda rhabdovirus OX=1481139 PE=4 SV=1
MM1 pKa = 7.43 SALEE5 pKa = 4.23 RR6 pKa = 11.84 IARR9 pKa = 11.84 SLSLKK14 pKa = 9.99 KK15 pKa = 10.44 LNPRR19 pKa = 11.84 RR20 pKa = 11.84 TPKK23 pKa = 8.86 TQPIPEE29 pKa = 4.5 KK30 pKa = 9.55 ATVYY34 pKa = 10.45 HH35 pKa = 7.05 PFMLSYY41 pKa = 10.97 DD42 pKa = 3.87 LNLAIEE48 pKa = 4.47 GKK50 pKa = 9.42 IHH52 pKa = 6.75 ISAITIIVNALSLAWAIEE70 pKa = 4.1 LFHH73 pKa = 7.5 SDD75 pKa = 4.12 SSWSGCLEE83 pKa = 4.09 YY84 pKa = 10.28 FWKK87 pKa = 10.57 SIKK90 pKa = 10.64 DD91 pKa = 3.7 NILASINPRR100 pKa = 11.84 VDD102 pKa = 3.48 PNGTCHH108 pKa = 6.2 MMTSIITFLGFSDD121 pKa = 4.9 GSCINSEE128 pKa = 4.12 AEE130 pKa = 3.84 PRR132 pKa = 11.84 QLTGSRR138 pKa = 11.84 SWEE141 pKa = 3.63 IMSPNQNLIVITLGFKK157 pKa = 8.95 ITLKK161 pKa = 10.11 TFAQHH166 pKa = 5.18 QRR168 pKa = 11.84 YY169 pKa = 8.97 SLRR172 pKa = 11.84 DD173 pKa = 3.4 HH174 pKa = 6.93 GFHH177 pKa = 6.83 KK178 pKa = 11.08 LEE180 pKa = 3.99 MLNEE184 pKa = 4.15 KK185 pKa = 8.95 EE186 pKa = 4.65 KK187 pKa = 11.37 KK188 pKa = 7.62 MLNYY192 pKa = 9.91 MGVKK196 pKa = 8.96 QLKK199 pKa = 8.38 PQYY202 pKa = 7.38 THH204 pKa = 6.55 EE205 pKa = 4.23 KK206 pKa = 8.95 TFEE209 pKa = 3.81 KK210 pKa = 10.8 LILKK214 pKa = 10.22 NKK216 pKa = 8.39 GPKK219 pKa = 9.36 GSRR222 pKa = 11.84 VRR224 pKa = 11.84 AILHH228 pKa = 4.88 SQSRR232 pKa = 11.84 DD233 pKa = 2.95 MWSPTAPSPPPTYY246 pKa = 10.53 EE247 pKa = 5.03 DD248 pKa = 3.88 GSSDD252 pKa = 3.88 EE253 pKa = 4.23 WDD255 pKa = 3.42 QQQLHH260 pKa = 6.22 SLNHH264 pKa = 5.73 LHH266 pKa = 6.15 TPSVPLRR273 pKa = 11.84 APRR276 pKa = 11.84 TSPPQQLSPKK286 pKa = 8.23 PTSTTQPLPQLTQPNKK302 pKa = 9.45 PQEE305 pKa = 3.96 LSKK308 pKa = 11.34
Molecular weight: 34.97 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 8.922
IPC2_protein 8.858
IPC_protein 8.756
Toseland 9.677
ProMoST 9.37
Dawson 9.867
Bjellqvist 9.531
Wikipedia 9.999
Rodwell 10.35
Grimsley 9.926
Solomon 9.897
Lehninger 9.882
Nozaki 9.721
DTASelect 9.502
Thurlkill 9.736
EMBOSS 10.072
Sillero 9.809
Patrickios 9.633
IPC_peptide 9.911
IPC2_peptide 8.053
IPC2.peptide.svr19 7.799
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
6
0
6
4069
109
2140
678.2
77.02
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
4.596 ± 1.018
1.425 ± 0.195
5.087 ± 0.481
5.235 ± 0.322
4.375 ± 0.603
4.792 ± 0.391
2.875 ± 0.175
6.906 ± 0.586
5.628 ± 0.274
11.969 ± 1.087
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.015 ± 0.284
4.989 ± 0.194
5.825 ± 0.453
4.178 ± 0.296
4.841 ± 0.32
8.675 ± 0.72
6.267 ± 0.753
5.48 ± 0.493
1.597 ± 0.254
3.244 ± 0.402
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here