Beihai narna-like virus 24
Average proteome isoelectric point is 8.15
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 2 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A1L3KI99|A0A1L3KI99_9VIRU RNA-dependent RNA polymerase OS=Beihai narna-like virus 24 OX=1922452 PE=4 SV=1
MM1 pKa = 7.59 ALVTDD6 pKa = 4.04 WTTAVGRR13 pKa = 11.84 LHH15 pKa = 7.59 RR16 pKa = 11.84 SPPKK20 pKa = 10.29 CAGDD24 pKa = 3.64 SALGRR29 pKa = 11.84 TGDD32 pKa = 4.25 PKK34 pKa = 11.12 DD35 pKa = 3.76 EE36 pKa = 4.59 PLGGTTGGGPPGPCGPDD53 pKa = 3.52 LLPTPPEE60 pKa = 3.97 GRR62 pKa = 11.84 HH63 pKa = 4.29 WGLRR67 pKa = 11.84 GDD69 pKa = 3.95 HH70 pKa = 6.21 GSLRR74 pKa = 11.84 NRR76 pKa = 11.84 EE77 pKa = 3.91 ASQVLLEE84 pKa = 4.33 HH85 pKa = 6.5 LTYY88 pKa = 10.15 STGDD92 pKa = 3.36 LPGTLEE98 pKa = 4.2 KK99 pKa = 10.6 EE100 pKa = 4.31 AGGKK104 pKa = 8.9 RR105 pKa = 11.84 VPVQDD110 pKa = 3.35 EE111 pKa = 4.26 QEE113 pKa = 4.34 LVRR116 pKa = 11.84 EE117 pKa = 4.32 AHH119 pKa = 6.5 CEE121 pKa = 3.67 AVLGIVTEE129 pKa = 4.26 VRR131 pKa = 11.84 PAPHH135 pKa = 6.58 EE136 pKa = 4.38 RR137 pKa = 11.84 DD138 pKa = 3.24 PALPGGDD145 pKa = 3.75 GPHH148 pKa = 6.74 LKK150 pKa = 10.53 LLARR154 pKa = 11.84 VVGPHH159 pKa = 5.17 VPVRR163 pKa = 11.84 DD164 pKa = 4.22 VPHH167 pKa = 6.51 HH168 pKa = 6.01 RR169 pKa = 11.84 CPRR172 pKa = 11.84 PGWIDD177 pKa = 3.33 QILPGSGPQEE187 pKa = 4.58 GGDD190 pKa = 3.96 PPPEE194 pKa = 4.12 PLRR197 pKa = 11.84 EE198 pKa = 4.11 SSTLWVPPHH207 pKa = 6.13 GRR209 pKa = 11.84 QPVPAQRR216 pKa = 11.84 GRR218 pKa = 11.84 RR219 pKa = 11.84 VDD221 pKa = 3.44 RR222 pKa = 11.84 KK223 pKa = 7.77 TAAPGPTMDD232 pKa = 5.4 ARR234 pKa = 11.84 MEE236 pKa = 4.5 DD237 pKa = 4.0 SQDD240 pKa = 3.3 PPHH243 pKa = 6.91 RR244 pKa = 11.84 PGVARR249 pKa = 11.84 LSTGCLHH256 pKa = 7.44 AVRR259 pKa = 11.84 HH260 pKa = 5.68 SDD262 pKa = 3.43 PYY264 pKa = 11.39 GKK266 pKa = 10.3 GLPVLRR272 pKa = 11.84 EE273 pKa = 3.87 VEE275 pKa = 4.27 RR276 pKa = 11.84 PNQATEE282 pKa = 3.8 GHH284 pKa = 6.36 KK285 pKa = 10.94 GKK287 pKa = 9.04 MVNPCAPPGVPRR299 pKa = 11.84 GLLHH303 pKa = 6.65 HH304 pKa = 7.3 PGRR307 pKa = 11.84 QEE309 pKa = 3.61 VRR311 pKa = 11.84 EE312 pKa = 4.28 GSLPWPATIYY322 pKa = 9.8 SSSVIVPHH330 pKa = 6.05 LHH332 pKa = 7.35 AEE334 pKa = 4.29 AEE336 pKa = 4.34 CLLRR340 pKa = 11.84 EE341 pKa = 4.29 HH342 pKa = 6.4 EE343 pKa = 4.27 VRR345 pKa = 11.84 PRR347 pKa = 11.84 LVVLASGEE355 pKa = 4.05 LPPTGLHH362 pKa = 6.76 DD363 pKa = 4.38 ALVPLDD369 pKa = 4.32 HH370 pKa = 7.56 RR371 pKa = 11.84 SGPNGRR377 pKa = 11.84 EE378 pKa = 3.37 IVPTYY383 pKa = 10.39 RR384 pKa = 11.84 RR385 pKa = 11.84 AGGCGGKK392 pKa = 8.0 TGQGDD397 pKa = 3.45 VTRR400 pKa = 11.84 NSRR403 pKa = 11.84 RR404 pKa = 11.84 PGPLGPVQVYY414 pKa = 7.71 QAEE417 pKa = 4.33 DD418 pKa = 3.52 SPGRR422 pKa = 11.84 RR423 pKa = 11.84 QPHH426 pKa = 6.25 HH427 pKa = 6.7 NPPLVHH433 pKa = 6.47 EE434 pKa = 5.15 LGPVGHH440 pKa = 7.78 LDD442 pKa = 4.15 RR443 pKa = 11.84 EE444 pKa = 4.76 GADD447 pKa = 3.14 QVGPNLVQMRR457 pKa = 11.84 RR458 pKa = 11.84 GASSGTAKK466 pKa = 10.56 DD467 pKa = 3.86 APDD470 pKa = 4.68 DD471 pKa = 4.02 GLAEE475 pKa = 4.24 VVRR478 pKa = 11.84 EE479 pKa = 4.04 EE480 pKa = 3.82 VGGRR484 pKa = 11.84 RR485 pKa = 11.84 EE486 pKa = 4.02 VRR488 pKa = 11.84 GHH490 pKa = 6.02 RR491 pKa = 11.84 KK492 pKa = 9.16 VLEE495 pKa = 4.31 EE496 pKa = 3.93 PHH498 pKa = 6.92 AGPTDD503 pKa = 3.7 QTTHH507 pKa = 6.73 RR508 pKa = 11.84 PCMVTVQDD516 pKa = 4.42 GLCPLVPPQSLEE528 pKa = 3.89 EE529 pKa = 4.38 PNAEE533 pKa = 4.03 EE534 pKa = 4.1 MSKK537 pKa = 9.45 IHH539 pKa = 5.91 QGPRR543 pKa = 11.84 ALGDD547 pKa = 3.8 YY548 pKa = 9.4 PDD550 pKa = 4.53 LSAAFRR556 pKa = 11.84 QRR558 pKa = 11.84 HH559 pKa = 5.84 RR560 pKa = 11.84 IDD562 pKa = 3.54 CYY564 pKa = 11.29 GPGEE568 pKa = 4.27 LRR570 pKa = 11.84 KK571 pKa = 9.57 LHH573 pKa = 5.86 QSLVTHH579 pKa = 6.18 QALTADD585 pKa = 4.08 DD586 pKa = 4.07 VDD588 pKa = 3.72 IAVCKK593 pKa = 10.23 ALHH596 pKa = 6.13 CVEE599 pKa = 5.35 PLPQGLPFWLRR610 pKa = 11.84 RR611 pKa = 11.84 FADD614 pKa = 4.18 RR615 pKa = 11.84 VCGPSRR621 pKa = 11.84 DD622 pKa = 3.74 VYY624 pKa = 11.18 GAGSKK629 pKa = 10.32 PSLGPGHH636 pKa = 7.89 LFTGATRR643 pKa = 11.84 QPSGRR648 pKa = 11.84 NNRR651 pKa = 11.84 PRR653 pKa = 11.84 EE654 pKa = 3.97 EE655 pKa = 4.13 AGRR658 pKa = 11.84 PPARR662 pKa = 11.84 PLFRR666 pKa = 11.84 GVQNGVRR673 pKa = 11.84 CGGWLGQLFTVLRR686 pKa = 11.84 EE687 pKa = 4.26 CCVTLAGTCLGKK699 pKa = 10.47 GASNIRR705 pKa = 11.84 EE706 pKa = 4.49 LEE708 pKa = 4.17 RR709 pKa = 11.84 PAVPVDD715 pKa = 4.21 DD716 pKa = 4.58 RR717 pKa = 11.84 PQRR720 pKa = 11.84 PSEE723 pKa = 4.14 VLGEE727 pKa = 4.21 ATTPPARR734 pKa = 11.84 NGDD737 pKa = 4.09 RR738 pKa = 11.84 LHH740 pKa = 6.97 HH741 pKa = 6.29 PQAQDD746 pKa = 2.92 SRR748 pKa = 11.84 PALEE752 pKa = 4.85 GGEE755 pKa = 4.22 CVIRR759 pKa = 11.84 SRR761 pKa = 11.84 EE762 pKa = 4.23 GGCPNQPPRR771 pKa = 11.84 QPGDD775 pKa = 3.25 PPRR778 pKa = 11.84 RR779 pKa = 11.84 LSSGMFRR786 pKa = 11.84 EE787 pKa = 4.34 VKK789 pKa = 9.66 PAEE792 pKa = 4.01 LVGRR796 pKa = 11.84 EE797 pKa = 3.93 ASEE800 pKa = 4.29 HH801 pKa = 5.6 CTEE804 pKa = 5.81 CPLQPDD810 pKa = 4.69 AIGRR814 pKa = 11.84 AGQLQARR821 pKa = 11.84 EE822 pKa = 4.25 EE823 pKa = 4.05 VRR825 pKa = 11.84 EE826 pKa = 4.01 AFPLGIRR833 pKa = 11.84 GRR835 pKa = 11.84 LPGTRR840 pKa = 11.84 AGTRR844 pKa = 11.84 PLRR847 pKa = 11.84 SVRR850 pKa = 11.84 HH851 pKa = 5.78 RR852 pKa = 11.84 GFGEE856 pKa = 3.94 KK857 pKa = 9.38 TPEE860 pKa = 4.73 AIGPARR866 pKa = 11.84 PNRR869 pKa = 11.84 SCVLQGG875 pKa = 3.32
Molecular weight: 94.41 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 7.33
IPC2_protein 7.6
IPC_protein 7.395
Toseland 7.117
ProMoST 8.024
Dawson 8.083
Bjellqvist 8.639
Wikipedia 7.98
Rodwell 8.112
Grimsley 7.205
Solomon 8.112
Lehninger 8.141
Nozaki 9.004
DTASelect 8.273
Thurlkill 8.317
EMBOSS 8.317
Sillero 8.697
Patrickios 4.787
IPC_peptide 8.112
IPC2_peptide 8.024
IPC2.peptide.svr19 7.704
Protein with the highest isoelectric point:
>tr|A0A1L3KI99|A0A1L3KI99_9VIRU RNA-dependent RNA polymerase OS=Beihai narna-like virus 24 OX=1922452 PE=4 SV=1
MM1 pKa = 7.25 EE2 pKa = 5.46 AVPVTSRR9 pKa = 11.84 RR10 pKa = 11.84 RR11 pKa = 11.84 CGFTKK16 pKa = 10.33 YY17 pKa = 10.07 FRR19 pKa = 11.84 GSLRR23 pKa = 11.84 SIIDD27 pKa = 3.23 RR28 pKa = 11.84 DD29 pKa = 3.66 CRR31 pKa = 11.84 ALQFSYY37 pKa = 10.69 VGRR40 pKa = 11.84 ALPEE44 pKa = 4.14 ASPGQCDD51 pKa = 3.44 AALTKK56 pKa = 10.51 YY57 pKa = 10.2 RR58 pKa = 11.84 EE59 pKa = 4.31 EE60 pKa = 4.22 LSQPATTPDD69 pKa = 3.98 SILDD73 pKa = 3.78 SAEE76 pKa = 3.25 EE77 pKa = 3.9 WARR80 pKa = 11.84 RR81 pKa = 11.84 WASRR85 pKa = 11.84 FLSGPVVPPTWLSGGACKK103 pKa = 9.68 EE104 pKa = 3.93 VPRR107 pKa = 11.84 AKK109 pKa = 10.5 GGLGTRR115 pKa = 11.84 SVDD118 pKa = 3.26 VARR121 pKa = 11.84 RR122 pKa = 11.84 ATDD125 pKa = 4.86 AICEE129 pKa = 4.0 PSEE132 pKa = 4.27 PEE134 pKa = 3.74 WEE136 pKa = 4.34 SLRR139 pKa = 11.84 EE140 pKa = 3.74 RR141 pKa = 11.84 LNAVEE146 pKa = 5.68 GLTDD150 pKa = 3.89 CDD152 pKa = 3.15 VDD154 pKa = 3.78 IVCRR158 pKa = 11.84 EE159 pKa = 3.87 RR160 pKa = 11.84 LVSNQALVEE169 pKa = 4.01 LSEE172 pKa = 4.41 LPRR175 pKa = 11.84 PIAVDD180 pKa = 3.83 AMALPEE186 pKa = 4.28 RR187 pKa = 11.84 GGKK190 pKa = 8.85 VRR192 pKa = 11.84 IVTKK196 pKa = 10.47 CPWALVYY203 pKa = 10.5 LGHH206 pKa = 7.15 FLRR209 pKa = 11.84 VWLLEE214 pKa = 3.46 GLRR217 pKa = 11.84 RR218 pKa = 11.84 DD219 pKa = 3.61 EE220 pKa = 4.17 RR221 pKa = 11.84 TKK223 pKa = 10.94 SVLDD227 pKa = 3.8 GDD229 pKa = 4.34 HH230 pKa = 6.71 AGSVGGLVGGAGVRR244 pKa = 11.84 LLEE247 pKa = 4.23 HH248 pKa = 6.66 LAVSADD254 pKa = 3.49 LTAASDD260 pKa = 4.19 LLPHH264 pKa = 6.94 DD265 pKa = 4.75 LCQAIVRR272 pKa = 11.84 GVLRR276 pKa = 11.84 GSRR279 pKa = 11.84 TRR281 pKa = 11.84 PAPHH285 pKa = 7.05 LDD287 pKa = 3.88 EE288 pKa = 4.58 VWADD292 pKa = 4.12 LIGPLTIQMPDD303 pKa = 2.57 GSEE306 pKa = 4.0 FVNQRR311 pKa = 11.84 GIMMGLPTTWTILSLVHH328 pKa = 6.76 LYY330 pKa = 8.44 WAEE333 pKa = 3.93 WAWASTVPRR342 pKa = 11.84 HH343 pKa = 5.91 ISLSGLPSAAPRR355 pKa = 11.84 TAICGDD361 pKa = 3.81 DD362 pKa = 4.58 LAAVWPTSVVQRR374 pKa = 11.84 YY375 pKa = 7.17 EE376 pKa = 4.55 SIVKK380 pKa = 10.05 SCGGQFSAGKK390 pKa = 8.94 HH391 pKa = 4.3 YY392 pKa = 10.76 KK393 pKa = 10.03 SRR395 pKa = 11.84 TYY397 pKa = 11.63 LMFTEE402 pKa = 4.32 EE403 pKa = 4.03 AFRR406 pKa = 11.84 LRR408 pKa = 11.84 VEE410 pKa = 3.8 MRR412 pKa = 11.84 DD413 pKa = 3.17 YY414 pKa = 10.36 DD415 pKa = 3.82 TRR417 pKa = 11.84 RR418 pKa = 11.84 VDD420 pKa = 3.12 GRR422 pKa = 11.84 RR423 pKa = 11.84 PGEE426 pKa = 3.7 ATLADD431 pKa = 5.02 FLPSWMVEE439 pKa = 4.04 EE440 pKa = 4.89 PSRR443 pKa = 11.84 DD444 pKa = 3.7 SRR446 pKa = 11.84 WCTGVDD452 pKa = 3.56 HH453 pKa = 7.05 LPLVPLRR460 pKa = 11.84 GLVRR464 pKa = 11.84 PLHH467 pKa = 5.93 LPKK470 pKa = 10.42 DD471 pKa = 4.11 RR472 pKa = 11.84 QPLPVWVAVPHH483 pKa = 5.9 CVEE486 pKa = 4.35 AACTQSGDD494 pKa = 3.12 AGAVRR499 pKa = 11.84 RR500 pKa = 11.84 ILRR503 pKa = 11.84 VLHH506 pKa = 6.07 PGIHH510 pKa = 5.53 RR511 pKa = 11.84 WARR514 pKa = 11.84 SRR516 pKa = 11.84 GFAVNAPTSLGGYY529 pKa = 7.78 GLPPVRR535 pKa = 11.84 GDD537 pKa = 3.42 PKK539 pKa = 10.32 RR540 pKa = 11.84 ARR542 pKa = 11.84 LPKK545 pKa = 8.24 WFRR548 pKa = 11.84 WGISSLLRR556 pKa = 11.84 ATPWKK561 pKa = 10.68 DD562 pKa = 3.67 LINPARR568 pKa = 11.84 AWAPVMRR575 pKa = 11.84 DD576 pKa = 2.81 IPHH579 pKa = 7.05 RR580 pKa = 11.84 DD581 pKa = 3.03 MGADD585 pKa = 3.28 HH586 pKa = 7.26 ASEE589 pKa = 4.06 KK590 pKa = 10.81 LKK592 pKa = 11.07 VGAVAARR599 pKa = 11.84 KK600 pKa = 9.83 GRR602 pKa = 11.84 VPFMGRR608 pKa = 11.84 GADD611 pKa = 3.42 LGYY614 pKa = 10.63 DD615 pKa = 3.32 AEE617 pKa = 4.65 DD618 pKa = 3.71 RR619 pKa = 11.84 LTVRR623 pKa = 11.84 LSNEE627 pKa = 3.6 LLFILDD633 pKa = 4.01 RR634 pKa = 11.84 DD635 pKa = 4.17 TLPSSFFLQGPRR647 pKa = 11.84 KK648 pKa = 9.91 VSGAVRR654 pKa = 11.84 KK655 pKa = 8.26 MFKK658 pKa = 9.16 QHH660 pKa = 5.94 LRR662 pKa = 11.84 RR663 pKa = 11.84 FPIPEE668 pKa = 4.13 GSVVASEE675 pKa = 4.53 TPMASLRR682 pKa = 11.84 RR683 pKa = 11.84 RR684 pKa = 11.84 WEE686 pKa = 3.89 EE687 pKa = 3.49 VRR689 pKa = 11.84 TARR692 pKa = 11.84 SWWSSPRR699 pKa = 11.84 GAAEE703 pKa = 4.02 GFVLGISSSAKK714 pKa = 9.42 RR715 pKa = 11.84 AVASALGWASMEE727 pKa = 4.28 PAHH730 pKa = 6.87 SGGPVSDD737 pKa = 3.85
Molecular weight: 81.42 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 8.97
IPC2_protein 8.697
IPC_protein 8.712
Toseland 9.311
ProMoST 9.209
Dawson 9.633
Bjellqvist 9.516
Wikipedia 9.794
Rodwell 9.765
Grimsley 9.663
Solomon 9.692
Lehninger 9.648
Nozaki 9.721
DTASelect 9.399
Thurlkill 9.531
EMBOSS 9.794
Sillero 9.692
Patrickios 5.385
IPC_peptide 9.677
IPC2_peptide 8.58
IPC2.peptide.svr19 7.737
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
2
0
2
1612
737
875
806.0
87.91
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
8.375 ± 1.162
2.295 ± 0.276
5.211 ± 0.246
6.328 ± 0.628
1.737 ± 0.586
10.422 ± 1.496
3.474 ± 0.814
2.295 ± 0.576
2.481 ± 0.257
9.491 ± 0.478
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
1.365 ± 0.373
1.303 ± 0.341
10.112 ± 1.942
3.04 ± 0.89
10.856 ± 0.001
5.955 ± 1.335
4.404 ± 0.044
7.754 ± 0.27
1.923 ± 0.93
1.179 ± 0.219
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here