Streptococcus merionis
Average proteome isoelectric point is 6.11
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 2137 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A239SLY0|A0A239SLY0_9STRE Uncharacterized protein OS=Streptococcus merionis OX=400065 GN=SAMEA4412692_00232 PE=4 SV=1
MM1 pKa = 7.62 ALAKK5 pKa = 9.78 IVYY8 pKa = 10.47 ASMTGNTEE16 pKa = 3.87 EE17 pKa = 4.51 IADD20 pKa = 3.59 IVSNKK25 pKa = 10.11 LEE27 pKa = 3.97 EE28 pKa = 4.66 LGVDD32 pKa = 3.7 VEE34 pKa = 4.72 TNEE37 pKa = 4.38 CTTVDD42 pKa = 4.19 ADD44 pKa = 4.69 DD45 pKa = 5.58 FLDD48 pKa = 4.26 ADD50 pKa = 4.23 LVIVATYY57 pKa = 9.31 TYY59 pKa = 11.24 GDD61 pKa = 3.83 GEE63 pKa = 4.56 LPDD66 pKa = 4.96 EE67 pKa = 4.49 IVDD70 pKa = 4.45 FYY72 pKa = 11.79 EE73 pKa = 6.45 DD74 pKa = 4.72 LLQLDD79 pKa = 4.97 LSGKK83 pKa = 9.94 IYY85 pKa = 10.01 GVCGSGDD92 pKa = 3.21 TFYY95 pKa = 11.45 DD96 pKa = 4.02 EE97 pKa = 4.52 FCKK100 pKa = 10.94 SVDD103 pKa = 3.45 EE104 pKa = 4.33 FDD106 pKa = 4.5 AAFTSTGATKK116 pKa = 10.23 GAEE119 pKa = 4.2 SVKK122 pKa = 10.33 VDD124 pKa = 4.94 LAAEE128 pKa = 4.52 DD129 pKa = 4.61 DD130 pKa = 4.8 DD131 pKa = 5.89 IINLEE136 pKa = 4.49 DD137 pKa = 3.56 FAQALAEE144 pKa = 4.15 HH145 pKa = 6.32 VLL147 pKa = 3.64
Molecular weight: 15.88 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.866
IPC2_protein 3.439
IPC_protein 3.452
Toseland 3.223
ProMoST 3.63
Dawson 3.452
Bjellqvist 3.605
Wikipedia 3.401
Rodwell 3.274
Grimsley 3.134
Solomon 3.427
Lehninger 3.389
Nozaki 3.567
DTASelect 3.795
Thurlkill 3.3
EMBOSS 3.401
Sillero 3.567
Patrickios 0.604
IPC_peptide 3.427
IPC2_peptide 3.541
IPC2.peptide.svr19 3.63
Protein with the highest isoelectric point:
>tr|A0A239SVE3|A0A239SVE3_9STRE LacI family transcriptional repressor OS=Streptococcus merionis OX=400065 GN=malR PE=4 SV=1
MM1 pKa = 7.45 KK2 pKa = 9.59 RR3 pKa = 11.84 TFQPSKK9 pKa = 9.26 IRR11 pKa = 11.84 RR12 pKa = 11.84 ARR14 pKa = 11.84 KK15 pKa = 8.35 HH16 pKa = 4.78 GFRR19 pKa = 11.84 NRR21 pKa = 11.84 MSTKK25 pKa = 9.22 NGRR28 pKa = 11.84 RR29 pKa = 11.84 VLAARR34 pKa = 11.84 RR35 pKa = 11.84 RR36 pKa = 11.84 KK37 pKa = 8.78 GRR39 pKa = 11.84 KK40 pKa = 8.62 VLAVSS45 pKa = 3.94
Molecular weight: 5.36 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.525
IPC2_protein 11.286
IPC_protein 12.881
Toseland 13.042
ProMoST 13.539
Dawson 13.056
Bjellqvist 13.042
Wikipedia 13.525
Rodwell 12.735
Grimsley 13.086
Solomon 13.539
Lehninger 13.451
Nozaki 13.042
DTASelect 13.042
Thurlkill 13.042
EMBOSS 13.554
Sillero 13.042
Patrickios 12.457
IPC_peptide 13.554
IPC2_peptide 12.53
IPC2.peptide.svr19 9.203
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
2137
0
2137
660612
29
2271
309.1
34.57
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
7.921 ± 0.063
0.577 ± 0.015
5.646 ± 0.049
6.796 ± 0.061
4.531 ± 0.043
6.584 ± 0.049
1.909 ± 0.023
7.224 ± 0.053
6.237 ± 0.06
10.328 ± 0.078
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.547 ± 0.027
4.221 ± 0.038
3.459 ± 0.029
4.211 ± 0.046
4.084 ± 0.039
6.12 ± 0.038
5.908 ± 0.054
7.035 ± 0.043
0.917 ± 0.019
3.741 ± 0.034
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here