Mycobacterium phage Rem711

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; Trigintaduovirus; unclassified Trigintaduovirus

Average proteome isoelectric point is 6.28

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 85 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A2K9VET1|A0A2K9VET1_9CAUD Uncharacterized protein OS=Mycobacterium phage Rem711 OX=2079285 GN=SEA_REM711_8 PE=4 SV=1
MM1 pKa = 7.35MPQTDD6 pKa = 4.17CVQCTVPATDD16 pKa = 4.25LDD18 pKa = 4.16GDD20 pKa = 4.23GLCRR24 pKa = 11.84FCRR27 pKa = 11.84DD28 pKa = 3.4YY29 pKa = 11.17TPPEE33 pKa = 3.71IVAQRR38 pKa = 11.84LDD40 pKa = 3.81VLEE43 pKa = 5.05LNCSTHH49 pKa = 6.89PDD51 pKa = 2.98QFLRR55 pKa = 11.84ANTICPKK62 pKa = 10.22CFCTPVPGQTGEE74 pKa = 4.12VGVVYY79 pKa = 10.33IDD81 pKa = 5.69DD82 pKa = 4.53GDD84 pKa = 3.99SDD86 pKa = 4.0ATEE89 pKa = 4.33ISCGDD94 pKa = 3.31TTVWLDD100 pKa = 3.32HH101 pKa = 6.22KK102 pKa = 9.71TDD104 pKa = 3.61TVSTNVGTTSAGIVGAEE121 pKa = 3.89ITPDD125 pKa = 4.63LIACLKK131 pKa = 10.17RR132 pKa = 11.84VIVEE136 pKa = 4.05RR137 pKa = 11.84AKK139 pKa = 11.06VGAA142 pKa = 4.17

Molecular weight: 15.18 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A2K9VEY2|A0A2K9VEY2_9CAUD Uncharacterized protein OS=Mycobacterium phage Rem711 OX=2079285 GN=SEA_REM711_43 PE=4 SV=1
MM1 pKa = 7.88RR2 pKa = 11.84EE3 pKa = 3.69AKK5 pKa = 9.43TRR7 pKa = 11.84IGAMLALVLIGLGASISAAFTRR29 pKa = 11.84PGGWLVLLAYY39 pKa = 10.18VAGAACAWLLVTALFAYY56 pKa = 9.75AQLRR60 pKa = 11.84QAVRR64 pKa = 11.84LRR66 pKa = 11.84EE67 pKa = 3.86ALNRR71 pKa = 11.84KK72 pKa = 8.62RR73 pKa = 11.84QTAEE77 pKa = 3.59LARR80 pKa = 11.84FLQQRR85 pKa = 11.84NAAGATPAVPPINEE99 pKa = 4.12SDD101 pKa = 3.62EE102 pKa = 4.27EE103 pKa = 4.24

Molecular weight: 11.08 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

85

0

85

16667

35

1359

196.1

21.17

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

12.66 ± 0.455

1.092 ± 0.149

7.074 ± 0.255

5.544 ± 0.273

2.784 ± 0.16

9.3 ± 0.607

1.728 ± 0.156

4.896 ± 0.203

2.988 ± 0.165

7.896 ± 0.29

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.848 ± 0.143

2.856 ± 0.174

6.756 ± 0.271

3.468 ± 0.222

7.452 ± 0.39

5.262 ± 0.214

5.808 ± 0.287

6.426 ± 0.234

1.938 ± 0.153

2.226 ± 0.134
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski